25 research outputs found

    Influence of different blood Glu cose control programs on prognosis and blood Glu cose in patients with intracerebral hemorrhage after operation together with stress hyperglycemia

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    目的 探讨不同血糖控制方案对脑出血术后应激性高血糖患者预后及血糖的影响。方法 采用回顾性研究方法将2015年6月~2016年5月入住我院重症医学科的52例脑出血术后应激性高血糖患者根据血糖控制方案的不同分为A组(n=25)和B组(n=27),A组采用静脉单次推注,B组采用持续静脉泵注。比较两组的28 d生存率、格拉斯哥预后评分、血糖达标率、达标时间、血糖监测次数、低血糖发生率、血糖水平和血糖变异度指标的差异。结果 两组的28 d生存率、血糖达标率、达标时间、低血糖发生率比较,差异无统计学意义(P〉0.05)。A组的格拉斯哥预后评分显著高于B组,差异有统计学意义(P〈0.05)。A组的血糖监测次数显著少于B组,差异有统计学意义(P〈0.05)。A组的血糖最大值(Glu_(max))和血糖差值(Glu_(dif))均显著小于B组,差异有统计学意义(P〈0.05)。结论静脉单次推注胰岛素能够改善脑出血术后应激性高血糖患者的神经功能预后,可以降低入ICU后血糖最大值和血糖差异。Objective To investigate influence of different blood glucose control programs on prognosis and blood glucose in patients with intracerebral hemorrhage after operation together with stress hyperglycemia.Methods A retrospective study was conducted,and 52 patients with stress hyperglycemia after cerebral hemorrhage in our hospital from June 2015 to May 2016 were divided into group A (n=25) and group B (n=27) according to different blood glucose control programs.Group A was treated with intravenous bolus injection,and group B was treated with continuous intravenous pump.The 28 d survival rate,the Glasgow outcome score,the blood glucose compliance rate,the standard time,the fre- quency of blood glucose monitoring,the incidence rate of hypoglycemia,blood glucose levels and blood glucose variabili- ty indexes were compared between the two groups.Results There was no significant difference in 28 d survival rate,the blood glucose compliance rate,the standard time,the incidence rate of hypoglycemia between the two groups (P〉0.05). The Glasgow outcome score in group A was significantly higher than that in group B,with significant difference (P〈 0.05).The number of blood glucose monitoring in group A was significantly less than that in group B,with significant dif- ference (P〈0.05).The Glu., and Glu~f in group A was significantly less than that in group B,with significant difference (P〈O.O5).Conclusion Intravenous single bolus insulin injection can improve the prognosis of nervous function in pa- tients with intracerebral hemorrhage after operation together with stress hyperglycemia,and can reduce the maximum blood glucose and blood glucose difference after ICU.广东省东莞市医疗卫生类科技计划一般项目(2015105101220

    Development and application of CK-MB specific monoclonal antibodies

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    本研究拟建立肌酸激酶同工酶MB(CK-MB)特异性单克隆抗体(mAb)的研制方法,对抗CK-MB单抗进行评价分类及性质鉴定,并初步建立CK-MB; 定量检测试剂。以CK-MB抗原免疫BALB/c小鼠,利用常规单抗制备技术,使用间接和捕获ELISA差异筛选法筛选单抗。利用肌酸激酶同工酶(CK-; MM/BB/MB)抗原对所制备单抗的抗原识别表位进行鉴定,另通过免疫印迹法及合成CK-MM、CK-BB差异性的线性表位肽鉴定对所制备的单抗进行评; 价分类。使用双抗体夹心ELISA方法筛选检测CK-MB抗原的配对mAb,并初步建立CK-MB定量检测试剂。使用74例临床标本初步评价该试剂与罗氏; 试剂的检测一致性。最终,我们成功筛选到22株稳定分泌抗CK-MB抗体的杂交瘤细胞株,这些单抗可以分为线性、偏构象的CK-MB和CK-MM或者CK; -BB交叉的单抗以及与CK-MB特异反应的偏构象型单抗,并使用偏构象型单抗研制出CK-MB定量检测试剂,该试剂与罗氏试剂相关系数r达到0.930; 9。综上所述,本研究建立了研制CK-MB偏构象型特异性单抗的筛选方法,通过对所筛选的单抗进行分析鉴定并建立了CK-MB定量检测试剂,与罗氏试剂检; 测结果符合率高。The aim of this study is to develop creatine kinase isoenzyme MB (CK-MB); specific monoclonal antibodies (mAb), and characterize the monoclonal; antibody and further development of quantitative detection assay for; CK-MB. The BALB/c mice were immunized with purchased CK-MB antigen, then; monoclonal antibodies were prepared according to conventional hybridoma; technique and screened by indirect and capture ELISA method. To identify; the epitopes and evaluate the classification, purchased creatine kinase; isoenzyme MB (CK-MM/BB/MB) antigen was used to identify the epitopes,; with immunoblotting and synthetic CK-MM and CK-BB in different linear; epitope. A double antibody sandwich ELISA was applied to screen the mAb; pairs for CK-MB detection, and the quantitative detection assay for; CK-MB was developed. We used 74 cases of clinical specimens for; comparison of our assay with Roche's CK-MB assay. We successfully; developed 22 strains of hybridoms against CK-MB, these mAbs can be; divided into linear, partial conformational CK-MB, CK-MM or CK-BB cross; monoclonal antibody and CK-MB specific reaction with partial; conformational monoclonal antibody, and CK-MB quantitative detection; assay was developed by using partial conformational monoclonal antibody.; The correlation coefficient factor r of our reagent and Roche's was; 0.930 9. This study established a screening method for CK-MB partial; conformational specific monoclonal antibody, and these monoclonal; antibodies were analyzed and an established quantitative detection assay; was developed. The new assay had a high concordance with Roche's.厦门市科技计

    A New Method and Device for Fast HBV Genotyping in Point-of-Care Diagnostics

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    介绍一种现场快速型HBV(乙型肝炎病毒)基因分型方法及装置,包括生物传感器与手持式荧光检测仪。为了提高检测灵敏度与准确性,采用荧光标记法。荧光检; 测仪采用简化方法来快速读取侧向流试纸上各测试线及质控线的信号幅值,基于预先建立的HBV基因分型模式匹配模型,实现快速分型。对48份HBV血清样本; 进行检测,实验结果表明,基于荧光检测仪及侧向流试纸的分型检测结果与传统核酸分析方法的分型检测结果一致,分型准确率达100%。与传统方法相比,现场; 快速型基因分型方法及装置可降低HBV分型检测的复杂度,缩短检测时间,降低检测成本,对乙肝个体化用药与个性化治疗具有重要意义。[无可用摘要]国家自然科学基金; 北京化工大学微系统与可穿戴医疗设备及生物传感技术创新研究团队资助项目; 福建省重点科技项

    低温胁迫对长苞铁杉幼苗的生理影响

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    经模拟低温(-12、-8、-4、0、4℃)胁迫处理后,长苞铁杉幼苗叶片和根系相关生理指标均发生明显变化.随着温度降低,叶片光合色素含量增加,但反映光合活性强弱的Chla/Chlb降低;叶片中渗透调节物质脯氨酸和可溶性糖含量增加,呈极显著正相关(r=0.804,p<0.01),根系中可溶性糖含量的减少说明根对低温胁迫的渗透调节更依赖于脯氨酸的累积.幼苗在-4℃及更低温度胁迫下受到严重的冻害使SOD活性持续下降,叶片中POD活性与SOD活性变化呈极显著负相关(r=-0.908,p<0.01),但根系中POD活性与SOD活性没有相关性,说明长苞铁杉幼苗对抗外界胁迫机理的复杂性.叶片MDA含量比根系低可能与叶片中Car能缓解1O2对细胞的伤害有关

    中国海及邻近区域碳库与通量综合分析

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    中国海总面积约470万平方公里,纵跨热带、亚热带、温带、北温带等多个气候带.其中,南海北依\"世界第三极\"青藏高原、南邻\"全球气候引擎\"西太平洋暖池,东海拥有全球最宽的陆架之一,跨陆架物质运输显著,黄海是冷暖流交汇区域,渤海则是受人类活动高度影响的内湾浅海.中国海内有长江、黄河、珠江等大河输入,外邻全球两大西边界流之一的黑潮.这些鲜明的特色赋予了中国海碳储库和通量研究的典型代表意义.文章从不同海区(渤海、黄海、东海、南海)、不同界面(陆-海、海-气、水柱-沉积物、边缘海-大洋等),以及不同生态系统(红树林、盐沼湿地、海草床、海藻养殖、珊瑚礁、水柱生态系统等)多层面对海洋碳库与通量进行了较系统地综合分析,初步估算了各个碳库的储量与不同碳库间的通量.就海气通量而言,渤海向大气中释放CO2约0.22Tg Ca-1,黄海吸收CO2约1.15Tg Ca-1,东海吸收CO2约6.92~23.30Tg Ca-1,南海释放CO2约13.86~33.60Tg Ca-1.如果仅考虑海-气界面的CO2交换,中国海总体上是大气CO2的\"源\",净释放量约6.01~9.33Tg Ca-1.这主要是由于河流输入以及邻近大洋输入所致.河流输入渤黄海、东海、南海的溶解无机碳(DIC)分别为5.04、14.60和40.14Tg Ca-1,而邻近大洋输入DIC更是高达144.81Tg Ca-1,远超中国海向大气释放的碳量.渤海、黄海、东海、南海的沉积有机碳通量分别为2.00、3.60、7.40、7.49Tg Ca-1.东海和南海向邻近大洋输送有机碳通量分别为15.25~36.70和43.39Tg Ca-1.就生态系统而言,中国沿海红树林、盐沼湿地、海草床有机碳埋藏通量为0.36Tg Ca-1,海草床溶解有机碳(DOC)输出通量为0.59Tg Ca-1;中国近海海藻养殖移出碳通量0.68Tg Ca-1,沉积和DOC释放通量分别为0.14和0.82Tg Ca-1.总计,中国海有机碳年输出通量为81.72~103.17Tg Ca-1.中国海的有机碳输出以DOC形式为主,东海向邻近大洋输出的DOC通量约15.00~35.00Tg Ca-1,南海输出约31.39Tg Ca-1.综上,尽管从海-气通量看中国海是大气CO2的\"源\",但考虑了河流、大洋输入、沉积输出以及微型生物碳泵(DOC转化输出)作用后,中国海是重要的储碳区.需要指出的是,文章数据是基于中国海各海区碳循环研究报道,鉴于不同研究方法上的差异,所得数据难免有一定的误差范围,亟待将来统一方法标准下的更多深入研究和分析.国家重点研发计划项目(编号:2016YFA0601400);;国家自然科学基金项目(批准号:91751207、91428308、41722603、41606153、41422603);;中央高校基础研究项目(编号:20720170107);;中海油项目(编号:CNOOC-KJ125FZDXM00TJ001-2014、CNOOCKJ125FZDXM00ZJ001-2014)资

    类泛素蛋白及其中文命名

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    泛素家族包括泛素及类泛素蛋白,约20种成员蛋白.近年来,泛素家族领域取得了迅猛发展,并已与生物学及医学研究的各个领域相互交叉.泛素家族介导的蛋白质降解和细胞自噬机制的发现分别于2004和2016年获得诺贝尔奖.但是,类泛素蛋白并没有统一规范的中文译名. 2018年4月9日在苏州召开的《泛素家族介导的蛋白质降解和细胞自噬》专著的编委会上,部分作者讨论了类泛素蛋白的中文命名问题,并在随后的\"泛素家族、自噬与疾病\"(Ubiquitinfamily,autophagy anddiseases)苏州会议上提出了类泛素蛋白中文翻译草案,此草案在参加该会议的国内学者及海外华人学者间取得了高度共识.冷泉港亚洲\"泛素家族、自噬与疾病\"苏州会议是由美国冷泉港实验室主办、两年一度、面向全球的英文会议.该会议在海内外华人学者中具有广泛影响,因此,参会华人学者的意见具有一定的代表性.本文介绍了10个类别的类泛素蛋白的中文命名,系统总结了它们的结构特点,并比较了参与各种类泛素化修饰的酶和它们的生物学功能.文章由45名从事该领域研究的专家合作撰写,其中包括中国工程院院士1名,相关学者4名,长江学者3名,国家杰出青年科学基金获得者18名和美国知名高校华人教授4名.他们绝大多数是参加编写即将由科学出版社出版的专著《泛素家族介导的蛋白质降解和细胞自噬》的专家

    一种深海潜水器的分层调压装置

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    本发明公开了一种深海潜水器的分层调压装置,潜水器外面的压强≤120MPa,潜水器里面的压强为0.1MPa,该深海潜水器包括n层球壳,n为大于1的整数,这些球壳具有同一个球心,相邻两层球壳之间的空间具有指定压强,相邻两层球壳之间的空间设置流体,并通过分层调压装置充入指定压强,所述分层调压装置是利用海水压强实现调压的装置。本发明相邻两层球壳之间的空间设置流体,并通过分层调压装置充入指定压强,所述分层调压装置是利用海水压强实现调压的装置,因此无需外加动力设备而不增加潜水器的重量,能够自我调节相邻两层球壳之间的压强,节省时间成本和人力成本,可靠性更高

    The deposition rate of sediments and biogenic elements in the restored wetland in the Yellow River Delta

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    Sedimentation rate plays an important role in wetland deposition because it indicates the storage capacity of wetland sediment and biogenic element. In this study, two sediment columns with a depth of 50 cm were collected in the restored wetland in the Yellow River Delta, and 10 soil physical and chemical properties were measured. The change point of the soil properties and the age since wetland restoration were used to determine the sedimentation rate. The results showed that the variables such as TOC, TN, delta~(13)C, and delta~(15)N, which were related to the accumulation of soil organic matter, were more suitable for calculating the deposition depth of the new layer of sediment in the restored wetland. As a result, the average sedimentation rate of the new layer in R2002 was calculated as 0.75 cm·a~(-1), and the deposition rates of C, N, and P were 208.65 g·m~(-2)·a~(-1), 20.74 g·m~(-2)·a~(-1), and 4.11 g·m~(-2)·a~(-1), respectively. In conclusion, the change point technique can be accurately used to determine the sedimentation rate of the new layer in restored wetland. In the future, the restored wetland will play more and more important role in sequester excess nutrient (such as N or P) due to human activities and global climate change

    用于升降机平层锁定的变杆长RSSR机构研究

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    在SS型施工升降机平层锁定机构中,开发了一种可变杆长的空间RSSR机构,通过串联平面正弦机构实现升降机平层锁定。该机构可依据升降机吊笼开门角度的大小,通过调整RSSR机构的杆长,保证吊笼门在不同的开门角度下,其正弦机构输出构件的位移量基本保持不变,实现平层机构的可靠工作。介绍了该装置的结构,建立了该组合机构的运动模型,给出了相关的设计计算过程,并结合实例进行了说明。研究工作为同类产品的开发提供可靠参考

    Dynamic responses and robustness performance to moving boundary of double-stepped cable during deep-sea mining

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    As the exploration and exploitation of deep-sea oil and gas, along with promising polymetallic nodule&sulfides mining, have been developing toward ultra-deep waters, some innovative concepts of marine cable configuration suitable for ultra-deepwater are proposed, such as stepped cable, hybrid cable and double-stepped cable. For deep-water cables with complex configurations, the structural responses become more complicated due to their non-uniform structural properties. Because the distributed buoyancy modules along cable length might introduce more significant local bending segments. Moreover, the impacts of moving boundary, caused by the motions of top vessel and bottom mining vehicle, should be considered. Through combing the finite element simulations with the hydrodynamic models, the dynamic response analysis approach of ultra-deepwater cables is established in this study. Then the double-stepped cable responses, including axial tension, displacement along with the change of overall configurations caused by moving top vessel and bottom mining vehicle, are calculated. Moreover, wave propagation behaviors during cable response are comprehensively examined, and the influences of non-uniform structural properties on cable response and wave propagation are analyzed using the wave propagation theory of structure with axially varying properties based on the Bessel function. The results show that the presented double-stepped cable can provide suitable configurations during the dynamic response, which has good compliance performance and can effectively buffer its response caused by moving boundary excitation. Finally, we found that the response spatial-temporal evolutions present some interesting phenomena, such as the axially non-uniform characteristics lead to non-monotonic changes in response amplitude and wavelength, with local peaks occurring in the low-tension region, owing to the distributed buoyancy modules, along with axiallyvarying and discontinuous structural properties. And, there exists significant mixed effect coming from both standing waves and traveling waves
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