The brand-building of the city-newspaper

论文摘要中国的都市类报纸在过去20多年的时间里发展十分迅速，特别是在1992年确立发展社会主义市场经济之后，作为国内市场化运作程度最高、对市民生活影响最大的报纸种类，都市类报纸经历了前期“跑马圈地”的扩张，和残酷的市场洗牌之后，开始进入以品牌建设为战略规划的阶段。本文试以商品的生命周期为线索，分别讨论处于生命周期不同阶段的都市类报纸如何进行品牌规划。论文主体分为五个部分。第一部分阐述都市类报纸诞生背景和现实发展状况。从报业经济的大环境入手，分析都市类报纸的发展脉络，得出21世纪，都市类报纸将以品牌来占领市场的主流发展空间；第二部分是界定报纸的品牌。介绍了国外大报在品牌建设方面的一些成功经验，和...AbstractDuring the last twenty years, the city newspaper media in china has been in rapid development. Especially after 1992 when socialist marketing economy has been regarded as a basic national policy, as a kind of newspaper which operates in high efficiency in marketing condition, and has an more impact on common people, the city newspaper media broke through nonstandard competition and elimina...学位：工商管理硕士院系专业：管理学院工商管理教育中心_工商管理硕士(MBA)学号：20031520

Endophytic and epiphytic Trichoderma of banana：diversity, population structure and biocontrol activities with banana wilt

通过对五种培养基的筛选获得了两种对木霉选择效率高，专一性好的木霉选择培养基（TSB和改良的TSM培养基）。利用这两种选择培养基，从香蕉（MusasapientumL.）根系中分离得到187株木霉，其中29株为内生木霉。香蕉木霉共分布在七个种内，表生木霉五个种，为棘孢木霉(Trichodermaasperellum)，深绿木霉(T.atroviride)，绿色木霉(T.virens)，哈茨木霉(H.lixii)，康氏木霉(T.koningii);内生四个种，为棘孢木霉（T.asperellum），短密木霉（T.brevicompactum），绿色木霉（T.virens），黄绿木霉（T.aureo...We chose two isolation media which can select Trichoderma spp from soil with high efficiency from five media. We isolated 187 Trichoderma spp from banana roots by the two select isolation media. There were 29 endophyte Trichoderma strains. Seven Trichoderma species were identified. There were T. asperellum, T. atroviride, T. virens, H. lixii, T. koningii, T. brevicompactum, T. aureoviride. Three d...学位：理学硕士院系专业：生命科学学院生物学系_微生物学学号：2172006115215

Recent Development on the Preparation of Monodisperse Nanoparticles

［中文文摘］单分散纳米微粒既可以在严格控制的条件下直接制备 ,也可以通过对多分散纳米微粒体系进行分级分离获得。本文在总结近年来国内外单分散纳米微粒的研究工作的基础上 ,介绍了直接制备和分级分离这两种获得单分散纳米微粒的方法。［英文文摘］Monodisperse nanoparticles can be obtained either by precisely controlled preparation,or through fractionating and separating of polydisperse nanoparticles.This paper provides information on monodisperse nanoparticles preparation by reviewing recent research works.福建省科技重点项目 ( 2 0 0 0 Ⅰ 0 15 );福建省青年科技人才创新项目 ( 2 0 0 1J0 5 6 )

Monoclonal antibodies against HPV11 virus-like particles: functional characteristics and application on quality assessment

目的分析和鉴定抗HPV11病毒样颗粒(virus-like particle,VLP)鼠源单克隆抗体的性质,筛选性质和生物学活性较优的抗体,并初步应用于抗原或疫苗的质量分析。方法分别利用间接ELISA法和Western blot对HPV11的22株单克隆抗体的亚类、与HPV11 VLP的结合能力和构象敏感性进行检测;采用血凝抑制实验对单克隆抗体的血凝抑制活性进行分析;运用基于假病毒的抗体中和实验对单克隆抗体的中和活性进行鉴定,选出中和活性高的单抗进行两两配对,采用双抗夹心ELISA法捕获单抗并筛选合适的配对双抗。结果对22株单抗的性质进行了详细和完整的鉴定,并根据构象敏感性进行排序,筛选出6株型别特异、结合活性强且中和活性高的单抗(2A2、4A1-3、16G7、14A6、9C12和19C7);成功建立了基于单抗的双抗夹心(14A6∶Ag∶9C12-HRP)ELISA定量分析方法。结论获得了较全面的HPV11 VLP单抗性质信息,建立了重组HPV11抗原质量分析的双抗夹心ELISA法,为HPV11抗原的生命周期管理或尖锐湿疣疫苗的研发、工艺优化、产品放行和稳定性研究等提供了技术支持。Objective To quantitatively analyze the characteristics of a panel of murine anti-human papillomavirus( HPV) 11 L1-derived virus-like particle( VLP) monoclonal antibodies( m Abs) and establish the m Ab-based methods for antigen quality analysis. Methods A panel of 22 murine anti-HPV11 m Abs were characterized in details with their isotype,and binding affinity,conformational sensitivity were examined quantitatively in the direct binding ELISA and Western blot. The hemagglutination inhibition activity of m Abs were identified using the hemagglutination inhibition assay and the pseudovirus( Ps V) neutralization efficiency were examined quantitatively using the Ps V-based neutralization assay. The type-specific, highly conformational sensitive and neutralizing m Abs were selected to be used in the sandwich ELISA assay. Results Based on the quantitative and semi-quantitative results,six type-specific,highly conformational sensitive and neutralizing m Abs( 2A2,4A1-3,16G7,14A6,9C1 and 19C7) were identified. These m Abs,along with 10D6 were screened as the capture m Ab or as the detection m Ab in the sandwich ELISA. Conclusion The binding affinity,conformational sensitivity and neutralization efficiency of anti-HPV11 m Abs were characterized in details. A m Ab-based sandwich ELISA assay( 14A6∶Ag∶9C12-HRP) were developed,which could be used in the in vitro potency analysis of HPV11 VLP-based vaccine.重大新药创制(2015ZX09101034

呼吸道合胞病毒L蛋白单克隆抗体的制备

目的:制备人呼吸道合胞病毒L蛋白单克隆抗体,并对其进行性质鉴定。方法:选取RSV-A2 L蛋白上具有亲水性可能的loop区段,将这些目的片段展示在HBc149上进行原核表达并纯化。重组蛋白免疫6~8周BALB/c雌性小鼠,根据免疫后血清的ELISA和WB分析结果选择L3935和L4099组小鼠进行脾脏免疫。融合后,用间接ELISA法筛选和多次克隆化,获得稳定分泌抗L蛋白单克隆抗体细胞株,并结合ELISA、免疫荧光和免疫印迹对所获得的单抗进行鉴定。结果:获得11株L蛋白特异性单克隆抗体,亚类鉴定结果 IgG1、IgG2a和IgG3单抗各3株,IgG2b单抗2株。结论:成功制备了L蛋白特异性单克隆抗体,也为RSV感染的免疫和发病机制研究奠定基础。国家自然科学基金(No.81401668)项

氧化镧表面反应性对甲烷和氧分子活化的影响

以氧化镧催化剂在甲烷氧化偶联(OCM)反应中的结构敏感性实验研究为基础,采用周期性密度泛函理论(DFT)计算研究氧化镧(001),(110)和(100) 3个晶面及OCM反应物分子甲烷和氧在其上的吸附、活化和解离...国家自然科学基金(批准号:21373169);; 教育部长江学者和创新团队发展计划项目(批准号:IRT1036)资助~

デジタルツールを用いたアニメーション制作効率の実践報告

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