Research on facial spectrum and color characteristics of five-zang disease status

目的:观察疾病状态人群面色光谱色度情况,为疾病状态五脏病评价提供可供依据。方法:采用日产柯尼卡美能达CM-2600d分光测色仪,观测健康组(183例)、疾病组(370例)额部、眉间部、鼻部、下颏、左右颧部、左右眼胞8处明亮度l、红光度A、黄光度b、饱和度C值及波长段(400~700nM)下的面色反射率值等指标,并据中医理论对疾病状态进行五脏病位分析。结果:疾病组l值显著低于健康组,b、C值均显著高于健康组;疾病组各波长段下反射率显著低于健康状态组(P<0.05)。疾病组除左右眼胞外的6个位点间的l、A、b、C值有显著性差异(P<0.05),不同点位有其特异性的色度变化特征。疾病组五脏病各组光谱色度特征比较有显著差异(P<0.05),结果与中医面部脏腑五色理论在一定程度上相符合。结论:运用光谱色度测定方法,可以作为诊断疾病状态五脏病的重要指标。Objective: To observe disease status facial spectrum and color,providing good quantitative basis for disease assessment.Methods: We gathered the facial color information in health(183) and disease groups(370) including L,a,b,C values and reflection of different wavelengths in 400-700nm with CM-2600D spectral photometric color measuring instrument on 8 points including frontal part,glabellas nose,mandible two cheeks and eye cells and analysized disease status five-zang positioning according to the principle of TCM syndrome differentiation.Results: L value of Disease group was lower than health status group and b,C values were higher than health status group,reflection of different wavelengths of disease group were lower than health group(P<0.05).6 points in disease group between loci L,a,b,C values with varying degrees of significant differences(P<0.05).Different point had its own specificity facial complexion variation characteristics.Facial spectrum and color of each five-zang disease group had significant difference through comparion of facial complexion.To a certain extent the result was consistent with TCM facial organs Five-color theory.Conclusion: There existed diagnostic value in distinguishing five-zang disease status in some degree by spectral photometric color measuring technique.国家科技支撑计划(No.2012BAI37B06);国家高技术研究发展计划(863计划)资助项目(No.2008AA02Z407);国家自然科学基金资助项目(No.30873463;No.81173200);国家自然基金青年项目(No.81102558);上海市重点学科(第三期)建设项目(No.S30302;No.S30303)---

EFFECT OF ALEXANDRIUM TAMARENSE ON POPULATION DYNAMICS OF BRACHIONUS PLICATILIS

采用单个体培养方法研究有毒赤潮藻塔玛亚历山大藻(Alexandriumtamarense)的细胞再悬浮液、细胞破碎液及其与亚心形扁藻(Platymonassubcordiformis)的混合藻液对褶皱臂尾轮虫(Brachionusplicatilis)实验种群动态的影响.结果表明,塔玛亚历山大藻及其细胞破碎液延缓了褶皱臂尾轮虫的生长发育,使轮虫的生殖前期延长,生殖期及寿命缩短,特定年龄出生率降低,产卵量减少,从而导致轮虫生殖力下降,种群增长受阻.其中以塔玛亚历山大藻细胞再悬浮液对轮虫的影响最为显著,rm仅为0.1832d-1.图1表2参18The effect of a toxic dinoflagellate, Alexandrium tamarense (strain ATHK) on the experimental population dynamics of the individual cultured rotifer, Brachionus plicatilis was studied. The experiment is divided into four treatments by culturing the rotifer in sand-filtered seawater with Platymonas subcordiformis as control;in sand-filtered seawater with A. tamarense cells as re-suspended treatment; in sand-filtered seawater with P. subcordiformis and broken A. tamarense as broken cells treatment; and in sand-filtered seawater with P. subcordiformis and A. tamarense as mixed cells treatment. The results showed that A. tamarense and its broken cells retarded the growth and development of B. plicatilis by prolonging its reproductive prophase, shortening its reproduction and life expectancy, reducing its natality in certain ages and decreasing the amount of spawned eggs. In all cases, the re-suspended A. tamarense cells produced the most significant adverse effect on the rotifer with the least r_ m (0.183 2 d -1 ). Fig 1, Tab 2, Ref 18教育部骨干教师基金项目;; 集美大学基金项目(C50209)资助~

A Medical Image Color Correction Method Based on Supervised Color Constancy

提出了一种室内自然光条件下的医学图像采集分析的“拓扑剖分-还原“(TrM)模型。该模型根据医学图像颜色特征,应用自制的彩色、灰度色标作为校正的监督色,在lAb(l*A*b*均匀色空间,简称lAb)色空间分别对一维l*空间和二维A*b*空间进行“拓扑剖分-影射还原“,并采用分段校正法进行l*值的校正,采用三角形拓扑剖分-重心逼近法进行A*b*值的校正。通过对不同条件下22幅图像的198个色块的校正结果显示,与白平衡校正比较,TrM模型校正后Δl*、ΔC*和ΔE明显降低(P<0.01),校正后图像色差更小,颜色与饱和度值更接近真实值。TrM模型校正方法能明显降低自然光条件下医学图像的色差,具有良好的颜色校正效果。This paper presents a medical image acquisition and analysis methodTRM(Topology Resolve-Map) Model-under natural light condition indoors.Firstly,in accordance to medical image color characteristics,a colorful and grayscale color control patch was made fou use as supervised color."Topology Resolve-Map-Restoration" was carried on in LAB color space of the one-dimensional L* space and the two-dimensional a*b* space.Then,L* value was regulated by subsection regulation and a*b* value was regulated by triangulation topological cutting close in on center of gravity method.After correction of the 198 color blocks in 22 pictures,the results showed that,by comparison with the standard value,the ΔL*,ΔC* and ΔE decreased significantly(P<0.01) after correction by TRM.After correction,the difference in image's color is reduced,the color saturation is improved and the value is closer to true value.TRM model can significantly reduce the color difference of the medical image under natural light condition;it has a good effect on color correction.国家高技术研究发展计划(863计划)资助(2008AA02Z407);国家自然科学基金资助项目(30873463;30300443);上海市重点学科资助项目(S30302

基于图像区域分割方法的舌质与舌苔识别

舌象的图像识别是舌象信息计算机诊断的主要内容之一。实现舌质、舌苔的区域识别是舌诊计算机识别过程中的重要步骤,也是后期舌体和舌苔颜色识别和纹理特征分析的重要前提。应用彩色图 像区域分割方法,根据舌象颜色的区域特征,采用分裂- 合并算法、色度阈值法对舌象进行了区域划分,建 立舌质、舌苔的区域分割方法,实现舌质与舌苔的分割。实验结果显示,分裂- 合并算法、色度阈值法具有 良好的分割效果。国家高技术研究发展计划(“863”计划)基金资 助项目( 2008AA02Z407 ) ; 国家自然科学基金资助项目(30873463, 30300443 ) ; 上海市重点学科建设资助项目( S30302

Progress in clinical application of tongue inspection objectivity based on digital image processing technique

舌诊作为中医望诊的重要组成部分,一直是临床辨证及疗效评价的重要依据之一,传统中医舌诊重于形象描述轻于客观量化的特点限制了中医诊断方法与技术的发展,然而随着近10年计算机技术的发展,数字图像处理技术在中医舌诊客观量化的研究中已取得了一定的成果,同时也被大量应用于临床研究。文章对近5年有关数字图像处理技术的舌诊客观化在疾病及相关指标、中医证候、疗效评价、健康与亚健康状态及体质的客观化辨识、中医舌诊检索技术方面的临床应用研究进行了概述,并且指出了其在临床应用研究中存在的问题与展望。As an essential component of inspection in traditional Chinese medicine(TCM), tongue inspection was always one of the important bases for clinical syndrome differentiation and efficacy evaluation.Traditional tongue inspection focused on image description and despised the objective quantitative, which limited the development of TCM diagnosis methods and technologies.With the development of computer technology in recent 10 years, it had achieved certain achievements in applying digital picture processing technique in objective researches of TCM tongue inspection, and the digital picture processing technique was also applied in clinical research widely.This article summarized the clinical application of digital picture processing technique in objective researches of TCM tongue inspection in related indicators of diseases, TCM syndromes, efficacy evaluation, objective identification of health and sub-health status and constitutions and TCM tongue inspection technologies, and pointed out the existing problems and prospects in its clinical application.国家自然科学基金项目(No.81173200;No.81102558;No.81373556); 国家科技支撑计划(No.2012BAI37B06); 上海曙光计划(No.12SG36)~

Application of Color Calibration Methods for the Tongue-color Images under Nature Indoor Light

目的建立一种室内非直射自然光条件下的医学图像颜色校正分析方法并初步应用。方法根据色彩还原算法结合自制彩色、灰度色标作为校正的监督色标建立TrM颜色校正方法,并对410幅舌色图像进行了校正。结果舌色A*、b*值除淡白舌组外,校正前后各组均有显著性差异(P<0.05),校正后A*、b*值离散度和变异系数较校正前减小。49例典型舌象校正后fISHEr'S分类方法与人工分类比较总体一致率75.5%,一致性检验结果kAPPA值为0.675(P<0.05)。结论不同舌象颜色经拓扑剖分-影射还原法校正后能较好的体现其l*A*b*颜色特征。拓扑剖分-影射还原模型校正方法能明显降低自然光条件下舌色图像的色差,具有良好的颜色校正效果。OBJECTIVE To establish a calibration analysis approach of medical image color under the circumstance of non-direct natural indoor light,and use it.METHODS TRM(Topology Resolve-Map) calibration method was established in accordance with the color reduction algorithm,with self-made color and grey patches being supervision color control patches;it was applied to correct 410 cases of clinical tongue image.RESULTS The before and after calibrated a *,b * values of each group's tongue color were significantly different(P<0.05) except for that of whitish group.Compared with pre-calibration,the dispersion and variation coefficient of post-calibration a*,b* values decreased.The overall consensus rate was 75.5% after using Fisher's classification method and artificial classification method to analyze 49 corrected typical tongue cases,with the Kappa value of consistency test result of 0.675(P<0.05).CONCLUSION The characteristics of L*a*b* in varied tongue colors can be better demonstrated after the calibration with TRM.TRM model approach can significantly reduce the color errors of tongue images under natural indoor light,which demonstrates its good color calibration effect.国家高技术研究发展计划(2008AA02Z407);国家自然科学基金(30873463;30300443

Literature Research on TCM Syndromes Distribution Characteristics of Menopausal Syndrome

目的研究围绝经综合征的中医证候分布特点,为规范本病的诊断和治疗提供依据。方法检索中国期刊全文数据库、中文科技期刊数据库、中国生物医学文献数据库、中国中医药数据库、万方数据资源系统1991-2011年发表的有关围绝经期综合征文献,进行文献整理分析。结果纳入分析文献191篇。围绝经期综合征常见中医证候有49个,其中肝肾阴虚、肾阳虚、肾阴虚、肾阴阳两虚、心肾不交、肝郁气滞、脾肾阳虚最多见;提取常见症状共65个,包括月经紊乱、头晕耳鸣、失眠、烘热汗出、腰膝酸软、心悸、易怒、纳呆等;病位以肾、心、肝、脾为主;病性以虚为主;脉象以细数、沉细、弦细为主,舌象以舌淡或舌红,苔薄或少苔为主。结论围绝经期综合征的中医证候分布比较集中,本研究为下一步临床科研工作奠定了基础。Objective To analyze TCM syndromes distribution characteristics of menopausal syndrome,provide evidence for further research.Methods The articles related to menopausal syndrome in CNKI Chinese Academic Journal database,Chinese Science and Technology Journal database,China Biological Medicine database,Chinese medicine database,Wanfang database during 1991-2011 were retrieved and analyzed.Results A total of 191 articles were included in the analysis,49 syndromes were found in menopausal syndrome,in which the common syndromes were liver and kidney yin deficiency,kidney yang deficiency,kidney yin deficiency,kidney yin and yang deficiency,heart and kidney deficiency,liver depression,spleen and kidney yang deficiency.We extracted a total of 65 common symptoms,including menstrual disorders,dizziness and tinnitus,insomnia,hot flashes sweeting,waist and knees,palpitations,irritability,poor appetite and so on.The common positions were kidney,heart,liver and spleen.The common disease characteristic was the deficiency-based.The common pulse were fine rapid,sunken fine,string-like fine.The common tongue were pale or red,the tongue fur were mainly moss thin or less moss.Conclusion The menopausal syndrome of TCM syndromes distribution characteristics were more concentrated,and this research laid a foundation for further clinical research work.国家高技术研究发展计划(2008AA02Z407);国家自然科学基金(30873463);上海市重点学科项目(S30302、S30303

Successive modification of polydentate complexes gives access to planar carbon-and nitrogen-based ligands

以碳和氮为键合原子的多齿螯合物是配合物家族中非常重要的一类。具有更高齿数的平面构型NC螯合物实例相对较少，代表性的例子为四齿金属碳卟啉类化合物，这类螯合物以其独特的结构和丰富的物理化学性质引起广泛关注。然而平面五齿、六齿的NC螯合物由于几何构型“拥挤”导致合成难度高，该工作从含三元环内金属卡宾结构的CCCC型碳龙配合物出发，利用经典有机反应（炔烃对金属卡宾的插入反应），成功地实现了CCCCN/NCCCN型平面五齿螯合物的合成。这一研究为高配位型螯合物的合成提供了新思路并为平面五齿螯合物家族添加新成员。特别是，这些高配位型螯合物在可见光和近红外区域均有较好的吸收，表现出良好的光声成像、光热转换及声动力学性能。 该研究工作在张弘教授指导下完成，第一作者为iChEM博士后周小茜。该工作充分体现了多学科协同研究优势：相关化合物的合成、表征及理论计算工作由周小茜博士完成；声动力学性能研究由厦门大学公共卫生学院庞鑫博士及刘刚教授完成；光声成像研究由厦门大学公共卫生学院聂立铭教授完成。iChEM fellow卓庆德博士、博士生卓凯玥、陈志昕参与了部分实验工作。夏海平教授、香港科技大学林振阳教授和南京大学朱从青教授对研究工作给予了大力支持。【Abstract】Polydentate complexes containing combinations of nitrogen and carbon (N and C) ligating atoms are among the most fundamental and ubiquitous molecules in coordination chemistry, yet the formation of such complexes with planar high-coordinate N/C sites remains challenging. Herein, we demonstrate an efficient route to access related complexes with tetradentate CCCN and pentadentate CCCCN and NCCCN cores by successive modification of the coordinating atoms in complexes with a CCCC core. Combined experimental and computational studies reveal that the rich reactivity of metal-carbon bonds and the inherent aromaticity of the metallacyclic skeletons play key roles in these transformations. This strategy addresses the paucity of synthetic approaches to mixed N/C planar pentadentate chelating species and provides valuable insights into the synthesis of carbon-based high-coordinate complexes. Furthermore, the resulting complexes are the examples of organometallic species with combined photoacoustic, photothermal, and sonodynamic properties, which makes them promising for application in related areas.This research was supported by the National Natural Science Foundation of China (Nos. 21572185, 21561162001, and 81571744), the Research Grants Council of Hong Kong (N_HKUST603/15), the Excellent Youth Foundation of Fujian Scientific Committee (2018J06024), and the Fundamental Research Funds for the Central Universities (20720170065).该工作得到国家自然科学基金委、香港研究资助局、福建省自然科学基金、厦门大学校长基金的资助