Design and Implementation of Bus Tickets Reservation System Based on.NET

随着我国经济持续的发展，信息化技术日益普及，铁路不断的改进服务方式，推出多种便民服务措施特别是高铁和城市轨道交通的快速发展，给客运行业带来了前所未有的竞争和压力。铁路和航空网络订票的乐章早已经在中国奏响，然而，汽车客运的票务工作却还处于纯手工化或微机化的运营模式，特别是乡镇和西部地区，汽车的运营变动性和随意性较大，乘客不仅无法预知汽车的运营状况，方便旅程的制定，购票和退票也需要在人工窗口进行，过程繁杂且低效，整个汽车订票服务用户体验很差，用户流失率较高。 在这样的背景下，研究我国基于.NET的汽车订票系统的应用，设计建设一个规模性的汽车订票系统，有着重大的意义。实现乘客网上订票、站内取票的快...With the continuous development of China's economy, increasing popularization of information technology, the railway improved service styles continuously, launched a variety of convenient service measures, especially the rapid development of high-speed railway and urban rail transit, bringing unprecedented competition and pressure to passenger transport industry .The movements of railway and airli...学位：工程硕士院系专业：软件学院_工程硕士(软件工程)学号：X201323200

Design and Implementation of Bank Intermediate Business Management System

随着利率市场化进程的加快，银行从中获得的收益将会越来越少，而银行如果将利率下调，客户的数量将会减少。所以在利率市场化的情况下，银行的利润空间势必会因为银行竞争力的提升而不断缩小。但近年来我国的地方商业银行逐渐崛起，这就让商业银行的竞争更加激烈，不少商业银行只能根据本地的经济情况来制定发展策略，并且以地方性的优势来求得发展。这样就导致很多商业银行的发展空间变小，业务的数量也就相应减少，导致利润空间缩小。中间业务的快速发展是商业银行生存的关键要素，开发一套中间业务管理系统显得尤为重要。 本论文的主要内容是设计并实现银行中间业务管理系统，结合目前我国商业银行中间业务管理的实际情况，首先对银行中间业...With the acceleration of the process of marketization of interest rates, banks will gain less and less, and if the bank will cut interest rates, the number of customers will be reduced. Therefore, in the case of interest rate market, the bank's profit margins will continue to shrink because of the competitiveness of banks. But in recent years, China's local commercial banks to gradually rise, whic...学位：工程硕士院系专业：软件学院_软件工程学号：X201323123

Advances in the methods of PCR-based mutagenesis with central overlap primers (COP)

基于PCR的体外诱变法发展至今，使用的引物从重组PCR法(RecombinationPCR)的至少4条引物到大引物PCR法(MegaprimerPCR)的3条引物，再到Quikchange快速突变法(QuikChangeMutagenesisPCR)的2条引物。从至少3轮PCR反应发展到只需2轮，到Quikchange法及反向PCR法（InversePCR）的仅使用一轮PCR反应，步骤越来越简单，操作越来越简便，为使用PCR技术进行各种基因克隆操作的科学工作者节省了大量的时间和精力。 近几年，有文献报道使用基于Quikchange原理的方法来制作DNA片段的插入、替代以及删除突变，但由于QC...With the development of PCR-based mutagenesis in vitro, the number of primer varied from at least 4 primers of Recombination PCR to 3 primers of Megaprimer PCR. When it comes to QuikChange Mutagenesis PCR, there are only 2 primers in use. It is improved from 3 rounds of PCR to only one round in Quikchange PCR and Inverse PCR. The great advances in PCR technology have saved a lot of time and energy...学位：理学硕士院系专业：生命科学学院生物医学科学系_细胞生物学学号：2172006115220

ヒドロキシシクロペンタジエニル9族金属錯体の合成と触媒機能

学位の種別:課程博士University of Tokyo(東京大学

An investigation on the detection of pigments using shell-isolated nanoparticle-enhanced Raman spectroscopy based on atomic layer deposition

以原子层沉积技术(ALD)制备出的壳层厚度为1; nm的Ag@Al_2O_3核壳纳米粒子为基底,采用壳层隔绝纳米粒子增强拉曼光谱(SHINERS)技术对色素检测进行探究。对Ag@Al_2O_3粒; 子的最佳粒径、色素检出限做了研究,并对实际样品中日落黄和柠檬黄的色素添加进行SHINERS检测。结果表明,对日落黄和柠檬黄具有最佳SHINERS; 效果的Ag@Al_2O_3粒子的最佳粒径为300 nm,方法对日落黄和柠檬黄标准溶液的检出限分别为10 mug/L和100; mug/L,对液体饮料中日落黄和柠檬黄标准品的SHINERS检出限分别为10 mg/L和50; mg/L,对所选实际样品中部分样品的日落黄和柠檬黄添加不符合国家标准。This study investigated the detection of pigment based on Ag@Al_2O_3; core-shell nanoparticles with shell-isolated nanoparticle-enhanced Raman; spectroscopy (SHINERS). The Ag@Al_2O_3 core-shell nanoparticles, which; shell thickness was 1 nm, was made by using atomic layer deposition; (ALD) technique. The study explored the best particle size of Ag@Al_2O_3; with best SHINERS effect and the SHINERS LOD of pigment. The method was; also applied to detect pigments in drink and food samples. The results; showed that the best size of Ag@Al_2O_3 nano particle with best SHINERS; effect of sunset yellow and lemon yellow was 300 nm, the SHINERS LOD of; standard solutions of sunset yellow and lemon yellow were 10 mug/L and; 100 mug/L,respectively,the SHINERS LODs of sunset yellow and lemon; yellow in drink were 10 mg/L and 50 mg/L,respectively, and sunset yellow; and lemon yellow in some of the selected real samples do not meet the; national standards.国家自然科学基金; 福建省自然科学基

从密斯到巴埃萨——探究极少主义理念的复杂性与多样性

20世纪初的极少主义思潮出现后,建筑的本质呈现与简洁明晰的空间体验成为众多建筑师所追寻的理想。代表人物密斯·凡·德·罗和继承其风格的坎波·巴埃萨,在极少主义思想的架构与表达上既一脉相承,又各有千秋。文章对两者空间生成中的共性与差异进行了总结归纳,并剖析了极少主义建筑理念的复杂性与多样性

Au Nanoparticles Based Colorimetric Detection of Conformational Changes in Cytochrome c

Corresponding author. Email: [email protected]; Tel: +86-592-2189663.[中文文摘]金纳米粒子(AuNPs)的颜色会随着细胞色素c(Cytc)构象变化而发生较大的变化,作者在改变原测定流程的基础上利用这种有规律的变化研究和测定了H+和L-半胱氨酸(L-Cys)对Cytc的构象变化.实验中分别加入pH=1-13的Cytc,可以使AuNPs显示青、蓝、紫、红等明显不同的颜色,从而可以利用AuNPs比色快速测定不同pH值的Cytc构象变化.在pH=7时,当附加L-Cys浓度从低浓度变化到高浓度时,AuNPs颜色逐渐从紫色变化到蓝色、青色,从而实现利用AuNPs比色测定由L-Cys所引起Cytc构象变化.圆二色(CD)光谱证实了pH=1-13和不同浓度L-Cys下的Cytc构象变化.借助紫外-可见吸收光谱和扫描电子显微镜(SEM)进一步明确了加入Cytc后AuNPs的不同聚集状态与其颜色变化的关系.[英文文摘]The colors of Au nanoparticles(Au NPs) change along with conformational changes in cytochrome c(Cyt c).We exploited this property for the colorimetric detection of Cyt c conformational changes induced by H + and L-cysteine(L-Cys).We improved the conventional procedure for this detection.After the addition of Cyt c within different pH values,the Au NPs are either cyan,blue,purple or red.This indicates that the Au NPs can be applied to the rapid colorimetric detection of pH-induced conformational changes in Cyt c conformational changes caused by interaction with L-Cys. The conformational changes of Cyt c were verified by circular dichroism (CD) spectroscopy. The relationship between the aggregation states and colors of the Au NPs after the addition of Cyt c was characterized by UV-Vis absorption spectroscopy and scanning electron microscopy (SEM).国家自然科学基金(20603027,20423002)资助项

Preparation and Photoeletrochemical Performance of CdS Quantum Dot Sensitized ZnO Nanorod Array Electrodes

Corresponding author. Email: [email protected]; Tel: +86-592-2189663[中文文摘]采用连续式离子层吸附与反应法制备了CdS量子点敏化的ZnO纳米棒电极.应用扫描电子显微镜(SEM)、X射线衍射(XRD)和透射电子显微镜(TEM)对CdS量子点/ZnO纳米棒电极的形貌、晶型和颗粒尺寸进行了分析和表征;采用光电流-电位曲线和光电流谱研究了不同CdS循环沉积次数及不同沉积浓度对复合电极的光电性能影响.结果表明,前驱体浓度都为0.1mol·L-1且沉积15次敏化后的ZnO纳米棒阵列电极光电性能最好.与单纯的ZnO纳米棒阵列电极和单纯的CdS量子点电极相比,其光电转换效率显著提高,单色光光子-电流转换效率(IPCE)在380nm处达到76%.这是因为CdS量子点可以拓宽光的吸收到可见光区,并且在所形成的界面上光生载流子更容易分离.荧光光谱实验进一步说明了光电增强的原因是,两者间形成的界面中表面态大大减少,有利于减少光生电子和空穴的复合. [英文文摘]We sensitized CdS quantum dots on a ZnO nanorod array electrode by the successive ionic layer adsorption and reaction method.Scanning electron microscopy(SEM),X-ray diffraction(XRD),and transmission electron microscopy(TEM) experiments were performed to characterize the morphology,crystalline phase,and grain size of the CdS quantum dot sensitized ZnO nanorod array electrodes.The effect of CdS deposition cycle number and the precursor concentration were studied by photocurrent-potential characteristics and photocurrent spectra. The results showed that the best photoelectrochemical performance was obtained at 0.1 mol·L-1 for both Cd2+ and S2- after 15 cycles. Meanwhile, the composite films exhibited a remarkably enhanced photoelectric conversion efficiency compared with the ZnO nanorods array films and with CdS quantum dot electrodes. The monochromatic incident photon-toelectron conversion efficiency (IPCE) was as high as 76% at 380 nm. This may be attributed to the broad light harvesting capability of CdS and the efficient separation of photogenerated carriers on its interface. The reason for this enhancement was further confirmed by a photoluminescent experiment. The results showed that sensitization with CdS quantum dots reduced the recombination of electron and hole pairs resulting in an enhancement in the photocurrent.国家自然科学基金(20433040