CoPt and NiPt Alloy Catalysts: Preparation, Self-Assembly, Electrocatalytic and Anomalous IR Properties

纳米材料具有不同于常规材料的特殊性质，在科学技术的发展中具有重要地位。纳米材料的合成，尤其是形状和结构控制合成、纳米材料的表征及其特殊性能的研究是纳米材料科学的前沿课题。CoPt和NiPt纳米材料由于其独特的磁学性能、高效的催化性能和特殊的光学性能而备受关注。本论文运用化学还原和电位置换法制备了不同结构的CoPt和NiPt纳米粒子，系统研究了它们的磁学、电催化和特殊红外光学性能。主要研究内容和结果如下：(1)一维链状CoPt纳米材料的合成及其性能研究。运用化学还原和电位置换法制备了两种不同结构的一维链状CoPt纳米材料，其一为实心结构(CoPt-a)，另一种为空心结构(CoPt-b)。磁性研究...Synthesis, especially the controlled synthesis, characterization and properties of nanomaterials are the most important tasks in nanoscience because they have unusual properties that could not possessed by corresponding bulk materials. CoPt and NiPt nanomaterials have attracted multidisciplinary attentions because of their special magnetic, catalytic and optical properties. In this paper, CoPt and...学位：理学博士院系专业：化学化工学院化学系_物理化学(含化学物理)学号：1912005140307

塔里木沙漠公路防护林土壤微生物生物量与土壤环境因子的关系

为探讨极端干旱区风沙土土壤微生物与土壤环境因子的作用规律,采用相关分析法研究了塔里木沙漠公路防护林地土壤微生物生物量与理化因子和酶活性的关系.结果表明:土壤容重和粒径减小(R0.85)时,防护林地中土壤微生物数量和生物量有增大趋势,由容重与微生物量的相关性主导;土壤养分含量与土壤微生物数量和生物量呈正相关,主要由速效养分和放线菌、微生物生物量C、P的相关性所致;土壤酶活性与土壤微生物数量和生物量的相关性差异较大,R在0.51～0.91,主要取决于蔗糖酶、磷酸酶与放线菌、微生物量C的相关;土壤盐分增加不利于土壤微生物生物量的积累(R0.63).实践中应为干旱区林地土壤微生物营造良好的土体,促进土壤物质循环

人口因素与未来20年中国高等教育规模变化的实证分析

20 0 2年教育部规划司重点委托项目;; 2 0 0 2年国务院人口普查办重点招标项目的资

Anomalous IR Optical Properties of Monodispersed PtNi Nanoparticles

采用电位置换反应以及化学还原法制备了单分散PTnI纳米粒子,循环伏安结果显示该纳米粒子在0.1MOl·l-1硫酸介质中对CO的氧化表现出比本体PT电极更好的电催化活性.以CO为探针分子,采用电化学原位红外光谱研究了PTnI纳米粒子上的特殊红外光学性能.结果表明,PTnI纳米粒子无论是在玻碳电极还是在金电极上,均表现出对称的双极谱峰,同时给出很强的增强效应.论文研究结果有助于进一步了解低维纳米材料特殊红外性能的本质.Monodispersed PtNi nanoparticles were synthesized by galvanic displacement reaction and chemical reduction.The monodispersed PtNi nanoparticles demonstrate, by cyclic voltammetry, enhanced electrocatalytic properties for CO oxidation in 0.1 mol·L-1 H2SO4 solution compared with bulk Pt electrode.In situ electrochemical Fourier transform infrared(FTIR) spectroscopy using CO as the probe molecule was studied.The CO adsorbed on either the PtNi/GC(glassy carbon) electrode or PtNi/Au electrode exhibits characteristics of a symmetric bipolar IR feature with a strong enhancement factor.The results of this paper contribute to the understanding of the special properties and origin of the anomalous IR properties of lowdimensional nanomaterials.supportedbytheNationalNaturalScienceFoundationofChina(21229301;21403126); ResearchFoundationofEducationBureauofHubeiProvince;China(D20131302)~

Establishment of MDCK cell lines which stably express visualable human neonatal Fc receptor

[目的]建立稳定表达融合EGFP的人新生儿Fc受体（h FcRn）的MDCK细胞株。[方法]构建重组慢病毒质粒p EGFP-h FcRn,采用四质粒包装系统共转染HEK 293T细胞生产重组慢病毒,感染MDCK细胞后对EGFP阳性细胞进行流式单细胞分选;通过Western Blot及EGFP-β2m荧光共定位验证h FcRn的完整性,并用流式细胞仪检测h FcRn与人Ig G的结合活性。[结果]测序结果表明成功构建p EGFP-FcRn慢病毒表达载体;感染后EGFP阳性MDCK细胞比例约为26.5%,流式单细胞分选后得到纯阳性细胞;荧光共定位及Western Blot均检测到h FcRn的完整表达;流式分析表明细胞株上的h FcRn与Ig G存在p H依赖性结合。[结论]成功获得稳定表达具有生物活性的可视化h FcRn的MDCK细胞株。[ Objective] To establish MDCK cell line stably expressing EGFP- human neonatal Fc receptor（hFcRn） fusion protein. [ Methods ] The lentiviral expression vector for EGFP - hFcRn fusion protein was constructed. Generating by co - transfection of four -plasmids into HEK 293T cells ,the lentivirus particles were used to infect MDCK cell line. EGFP positive single cell was obtained by FACS, and then FcRn expression was identified by fluorescence co -location with EGFP - β2m and confirmed by Western Blot. Flow cytometry was used to detect binding activity of hFcRn and human IgG. [ Results ] DNA se- quencing demonstrated that the lentivirus vector pEGFP - FcRn was constructed successfully. The percentage of EGFP - posi- tive ceils was about 26.5% after infection. Expression of the complete protein was detected through fluorescence co - location and Western Blot, respectively. Flow cytometry analysis showed that the cell lines could pH - dependently capture human IgG. [ Conclusion] MDCK cell line stably expressing functional visualable hFcRn was established.基金项目：国家自然科学基金项目（“结构生物学指导的HBV治疗性抗体人源化及其关键技术研究”,No.31600748;“抗呼吸道合胞病毒高中和活性抗体的保护机制研究”,No.81401668;“基于广谱中和单抗的通用型流感疫苗设计及其结构基础研究”,No.31670934

教材语言调查统计方法的新发展——基于基础教育新课标人教版、苏教版、北师大版、语文版的比较

对于任何一门科学而言,其成熟的主要标志是研究方法的科学化、系统化。在教材语言研究的过程中,开始我们多使用频次、文本数和分布率、累计频率等统计方法。由于教材语言具有基础性、有限性和有序性的特点,我们进一步提出教材语言统计的新方法,即复现调查法、使用度调查和频率差等教材语言统计方法。调查方法的发展和演进,有助于我们对教材语言的特点、性质和面貌有更进一步的认识

Studies on changes in the ratio of free to total PSA after endocrine treatment of prostate carcinoma

前立腺癌症例の内分泌療法に伴う血中PSAの変化をfree PSAとtotal PSAに分け検討した.治療開始後3～4ヵ月のPSAの減少率はfree PSAよりもtotal PSAで大きいことが示された.即ち内分泌療法に伴うPSAの減少はfree PSAよりもcomplex PSAで大きいことが示されたThe post-diagnostic changes in the free to total PSA ratio in the serum of patients with prostate carcinoma, after the initiation of endocrine treatment were examined. Two-week pretreatment with either chlormadinone acetate (100 mg/day) or flutamide (375 ng/day) was administered orally to 14 patients with newly diagnosed advanced prostate carcinoma (clinical stage was C in 2, Dl in 1 and D2 in 11). Then the LH-RH analogue was injected. Total and free PSA in the serum of these patients were measured every 4 weeks by the Ab bead PSA (Eiken) and the recently developed assay for free PSA by Eiken, respectively. The follow-up period ranged from 3 to 9 months with a median of 6 months. Levels of both total and free PSAs decreased significantly following the endocrine treatment, while free to total PSA ratio at 4 to 16 weeks after the start of LH-RH analogue was increased significantly compared to the pretreatment level (p < 0.05). These findings suggest that the rate of decrease of complex PSA during the first 4 months after the beginning of treatment may exceed that of free PSA in the serum of patients with advanced prostate carcinoma initially treated with endocrine therapy

Therapeutic effects comparison between platelet-rich plasma and sodium hyaluronate intra-articular injection in treatment of knee osteoarthritis

目的关节腔内注射自体富血小板血浆（PRP）与透明质酸钠（SH）,观察并比较其疗效。方法于2012年2月至12月,漳州市解放军第175医院骨科收治的81例Kellgren-Lawrence（KL）分级标准为Ⅱ、Ⅲ级膝关节骨关节炎的患者,年龄48～60岁,平均（52.6±3.6）岁;男性27例,女性53例;左膝38例,右膝43例。K-L分级标准,Ⅱ级47例,Ⅲ级34例,按就诊顺序序贯随机平行分为SH组（40例）和PRP组（41例）,分别采用SH和PRP关节腔内注射进行治疗。治疗前和治疗后3、6、9、12个月对患者进行随访并进行美国西部Ontario与Mc Master大学骨关节炎指数（WOMAC）评分评估,两组间各时间点WOMAC评分比较采用t检验,两组内各时间点WOMAC评分比较采用重复测量方差分析;分别于治疗前和治疗后12个月进行膝关节磁共振检查,评价关节腔内积液的吸收情况。结果治疗前及治疗后3个月PRP组WOMAC评分[（48.42±4.95）分,（25.38±5.12）分]和SH组[（47.17±5.12）分,（25.17±5.09）分]比较,t值分别为-0.625和0.413,差异无统计学意义（P均〉0.05）;治疗后6、9、12个月PRP组WOMAC评分[（21.44±4.73）分,（20.54±4.46）分,（16.53±7.35）分]和SH组[（25.87±5.16）分,（27.22±4.21）分,（29.63±8.21）分]比较,PRP组低于SH组,t值分别为-2.765、-3.447和-4.657,差异具有统计学意义（P均〈0.05）,治疗后12个月复查磁共振,可见PRP组的关节积液吸收例数高于SH组,差异有统计学意义（P〈0.05）。结论膝关节腔内注射自体PRP,临床效果优于透明质酸钠,中长期疗效好。Objective To compare the efficacy of platelet rich plasma （PRP） and sodium hyaluronate （SH） intra-articular injection in the patients with knee osteoarthritis of grade Ⅱand Ⅲ. Methods A total of 81 patients with knee osteoarthritis of grade Ⅱ and Ⅲ （ Kellgren-Lawrence standard） were randomly assigned to the PRP and SH group. All 81 patients were treated in the 175th hospital of PLA, Zhangzhon, with the average age of （52.6 ± 3.6） years. There were 27 males and 53 females. Forty cases in SH group and 41 cases in PRP group had got the complete follow-up. Both the two groups adopted 4 ml of 5% lidocaine for intra-articular injection. All the patients were prospectively evaluated before the treatment and at the 34 , 6th , 9th and 12th month after the treatment by the Western Ontario and McMaster Universities （WOMAC） osteoarthritis index. The swelling subsiding knee was observed. Between the two groups WOMAC scores were compared using t test at each time point, within the groups the scores were compared by repeated measures analysis of variance. The absorption of accumulated fluid in the joint cavity was observed and compared by MRI before and 12 months after the treatment. Results No serious adverse event was detected in the two groups. According to the follow-up evaluations, both the groups presented clinical improvements. The comparison between the two groups showed no statistically significant difference before therapy and three months after the treatment （ t =- 0. 625, 0. 413, P 〉 0. 05 ）. Six months, nine months and 12 months after the treatment, WOMAC score in the PRP group[ （21.44 ±4. 73）, （20. 54 ± 4. 46） ,（16. 53 ± 7. 35 ） pointsl were lower than the SH group[（25.87 ±5.16）, （27.22 ±4.21）, （29. 63 ± 8.21 ） points] ; the difference was statistically signifieant（ t = - 2. 765, - 3. 447, - 4. 657, P 〈 0. 05 ）. Conclusion PRP intra-artieular injection in the treatment of knee osteoarthritis of gradeⅡ and Ⅲ may be more suitable compared to南京军区医学科技创新重点课题项目（15ZD027

估计葡萄糖处置率与冠状动脉狭窄严重程度关系的横断面研究

目的探讨估计葡萄糖处置率（eGDR）与冠心病（CAD）严重程度的关联。方法采用以医院为基础的横断面研究设计，纳入因疑似冠心病而接受冠状动脉造影检查的患者共1 258人（平均年龄：62（53~68）岁；男性占53.9%）。按照eGDR公式计算胰岛素抵抗水平（IR）：eGDR =21.158 - ［0.09×腰围（WC， cm）］ - ［3.407×高血压（hypertension， 是/否）］ - ［0.551×糖化血红蛋白（HbA1c， %）］。根据eGDR三分位数对研究对象进行分组。冠心病的严重程度由狭窄血管的数量决定： 无明显CAD组（所有冠脉狭窄均&lt;50%，n=704），单支血管CAD组（只有一条受累的主要冠脉狭窄≥50%，n=205），多支血管CAD组 （两条或两条以上受累的主要冠脉存在狭窄≥50%，n=349）；以无明显CAD作参照，采用多因素logistic回归模型分析eGDR与CAD严重程度之间的关联。采用限制性立方样条分析eGDR和CAD在整个eGDR范围内的线性关联。采用亚组分析评估不同糖尿病状态下eGDR和CAD严重程度之间的关联。受试者工作特征（ROC）曲线分析eGDR对提高CAD筛查模型的价值。结果eGDR降低与CAD严重程度的风险增加显著相关。（OR：2.79；95%CI：1.72~4.55；P&lt;0.001）。多因素logistic回归模型中，eGDR最低分位（T1）的个体患多支血管CAD的风险是eGDR最高分位（T3）的2.79倍。（OR：2.79；95%CI：1.72~4.55；P&lt;0.001）。限制性立方样条分析显示，eGDR与CAD以及多支血管CAD之间存在负线性关联（P-linearity&lt;0.05）。在非糖尿病患者中，与参照组（T3）相比，T1组患CAD和多支血管CAD的风险显著增加，OR分别为1.42 （95% CI：1.00~2.01；P&lt;0.05）和1.86 （95%CI：1.21~2.86；P&lt;0.05）。而在糖尿病患者中未发现此关联有统计学意义（P&gt;0.05）。ROC曲线分析，eGDR加入到CAD传统筛查模型中时，AUC、IDI、NRI的结果显示，模型对CAD和多支血管CAD的筛查有显著改善。结论eGDR与CAD及CAD严重程度呈负相关。eGDR作为一种无创且易于获取的非胰岛素测量指标，具有筛查大规模人群中CAD严重程度的潜在价值