油酰乙醇胺对高脂血症大鼠降血脂及肝脏的保护作用

目的观察油酰乙醇胺(OEA)对高脂血症模型大鼠降血脂及肝脏保护作用。方法高脂饮食建立高脂血症大鼠模型,分别观察OEA(10,203,0 mg/kg)对高脂血症大鼠的血清胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、谷丙转氨酶(ALT)、肝重和肝脏系数、肝脏丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)的影响。制作冰冻切片观察大鼠肝脏脂质变性程度。结果与模型组相比,OEA(20,30mg/kg)具有降血脂作用,同时降低血清ALT、肝脏脂质、肝重和肝脏系数、肝脏MDA水平,升高肝脏GSH-Px活力。结论 OEA能降低高脂血症大鼠血脂、抑制肝脏脂肪沉积,并减轻脂质过氧化物对肝脏的损伤

左侧颈内静脉置入带涤纶套导管尖端误入右侧头臂静脉1例

患者:男性,60岁,因"血液透析9年,寒战伴发热6月余"于2017年1月31日收入院。患者9年前因"慢性肾炎,慢性肾脏病CKD 5期"于右侧颈内静脉行带涤纶套导管置入术建立通路,开始接受规律性血液透析治疗,平时一般情况尚可。2012年10月曾因导管感染给予更换带涤纶套导管一次,2013年1月又因该导管出现裂痕渗血,于原位置再次更换带涤纶套导管,使用至今。6个月前开始于透析过程中出现寒战、发热(体温38℃～40℃),考虑颈内静脉带涤纶套导管感染,给予头孢类抗生素+肝素混合溶液透析间期封管治疗后好转,但上述症状反复出现。1个月前于当地

Construction of a Recombinant Escherichia Coli DH5α for Dextranase Expression

以Plf3为模板,PCr扩增得到葡聚糖酶的启动子,并将启动子基因重新连接到切除启动子基因及葡聚糖酶基因的Plf3载体上,构建Plf3-PrO载体.采用PCr的方法扩增朱黄青霉总dnA中的α-1-6-葡聚糖酶基因,并插入到Plf3-PrO载体上,构建Plf3-PrO-dEX质粒,以大肠杆菌dH5α为宿主菌,酶切验证及PCr验证均表明成功构建了Plf3-PrO-dEX质粒,且重组质粒中的α-1-6-葡聚糖酶基因与朱黄青霉中的α-1-6-葡聚糖酶基因有93%的同源性.The promoter of dextranase was PCR-amplified from pLF3 vector and inserted into pLF3 plasmid,from which the genes encoding a promoter and β-glucanase had been removed,yielding PLF3-Pro.The DNA encoding the dextranase was PCR-amplified from the total DNA of Penicillium minioluteum MUCL 38929 and cloned into pLF3-Pro vector.The recombinant plasmid pLF3-Pro-Dex was constructed and checked through PCR,restriction enzyme digestion and DNA sequencing.Results shows that the recombinant plasmid pLF3-Pro-Dex is successfully constructed.科技部科研院所技术开发研究专项资金(NCSTE-2007-JKZX-023

Rethinking the Ideological Connection between Marx and Hess

黄其洪，西南大学马克思主义理论研究中心暨西方马克思主义研究所教授、博士生导师；卢丽娟，西南大学马克思主义理论研究中心博士研究生。【中文摘要】马克思与赫斯的思想关联存在着友好合作、决裂告别、隔空对话三个时期，以往学界只讨论了马克思与赫斯之间的友好合作和决裂告别两个时期,却完全忽略了二者之间的隔空对话期。在晚年时期，马克思和赫斯分别给予了对方的《物质动力学说》和《资本论》以崇高的礼赞。这说明他们之间的“决裂”并非简单意义上的一次性的彻底决裂,而是经历了 “友好一决裂一对话”的发展轨迹。二者早期的决裂究竟是在什么意义上发生的，在决裂的同时有没有保持一致性，在何种意义上具有一致性,这种一致性是不是导致二人在晚年再次相互欣赏的原因，学界对这些问题一直缺乏深入的讨论。事实上,赫斯曾经一度在费尔巴哈的类哲学那里停留而忽略了辩证法的革命意义，而马克思却并没有在费尔巴哈那里过多的停留，这是导致二者在1848年发生所谓“决裂”的原因。赫斯在晚年体会到了辩证法的革命性和科学性,从而挣脱了费尔巴哈的思想束缚,与马克思殊途同归，走向了一种类似于历史唯物主义的道路。这再次说明，历史辩证法是历史唯物主义的关键,脱离开历史辩证法去抽象地讨论历史唯物主义是一种严重的方法论错误，这一点对于我们反思当代的西方马克思主义具有重要的警示作用。 【Abstract】The relationship between Marx and Hess went through three periods: friendly cooperation，breakup and the “trans-time-and-space” dialogue. In the past，the academic circles only discussed the friendly cooperation and break-up between Marx and Hess，but completely ignore the “trans-time-and-space” dialogue period. In their later years，Marx and Hess sang high praise of each other's works - The Theory of Material Dynamics and Capital. This shows that the “breakup” between them was not a one - time complete breakup in the simple sense， but a development trajectory of “friendship-breakup-dialogue”.In what sense did the early breakup of the two happen?Whether there was a consistency in the breakup? In what sense did the consistency show up? Did this consistency make the two appreciate each other again in their later years? The academic circles have few in-depth discussions on these issues. In fact， Hess once lingered in Feuerbach's species-philosophy and ignored the revolutionary significance of dialectics，but Marx did not delve deeply in Feuerbach，leading to the breakup between the two in 1848.In his later years，Hess came to recognize the revolutionary and scientific nature of dialectics，thus breaking free of Feuerbach's ideological bondage and moving in the same direction as Marx to embark on a path similar to historical materialism. This again shows that historical dialectics is the key to historical materialism. It is a serious methodological error to abstractly discuss historical materialism while being detached from historical dialectics.This has an important warning effect on our reflection on contemporary Western Marxism.重庆市社科基金项目一般项目“马克思的实践观念及其在当代中国的发展研究”（2014YBZX009)；重庆市社科基金项目一般项目“马克思低阶正义理论研究”（2016YBZX012)西南大学中央业务费重大培育项目“中国特色社会主义的历史哲学定位及世界意义研究”（SWU1809010 )西南大学中央业务费重点项目“马克思社会主义社会正义观研究”（SWU1909313

Cloning of a Recombinant Dextranase and Its Expression in Pichia pastoris

通信作者: [email protected][中文文摘]根据朱黄青霉(Penicillium minioluteumHI-4)α-1,6-葡聚糖酶的基因序列,人工合成α-1,6-葡聚糖酶基因并将其插入毕赤酵母(Pichia pastoris)表达载体pPICZαA,PCR和双酶切验证结果均表明成功构建了重组质粒pPICZαA-Dex.重组质粒经SacⅠ线性化后整合到毕赤酵母X33基因组中进行表达.摇瓶发酵表明,重组毕赤酵母经甲醇诱导120 h产酶活力达到最高值29.2 U/mL,SDS-PAGE结果显示在67 ku附近有明显的条带,与α-1,6-葡聚糖酶理论分子质量相符合.[英文文摘]The α-1,6-dextranase gene was amplified from a synthetic α-1,6-dextranase gene derived from Penicillium minioluteum HI-4 and was inserted into plasmid pPICZαA.The recombinant plasmid named pPICZαA-Dex was identified by PCR and enzyme digestion,and was then electrotransformed into Pichia pastoris X33.The recombinant Pichia pastoris X33 was induced to express the dextranase using methanol.The enzyme activity reached 29.2 U/mL after 120 h of induction.The size of the recombinant α-1,6-dextranase is approximately 67 ku analyzed by SDSPAGE, which is consistent with the theoretical molecular weight.科技部科研院所技术开发研究专项资金(NCSTE-2007-JKEC-023

Design of wireless sensor data processing systemfor border management and control based on artificial intelligence

边境管控在国防安全和国民经济社会发展中具有重要作用。针对目前边境管控的严峻形势,提出了运用无线传感网络技术建立能获取跨境事件位置和相关信息的传感网络,利用人工神经网络技术,设计了基于人工智能的边境传感信息处理系统,并用Matlab进行了仿真实验。实验结果表明这一边境传感信息处理系统稳定可靠,具有较高的准确率,可以提升边境传感信息的价值含量,协助边境管理部门提高边境管控能力和效率。Border control plays an important role in national defense security and national economic and social development. Aimingat the grim situation of border control, a wireless sensor network is proposed to acquire the location and related information ofcross-border events. Using artificial neural network technology, a border sensor information processing system based on artificialintelligence is designed. Simulation experiments are carried out with Matlab. The experimental results show that the system is stableand reliable, and has higher accuracy. It can enhance the value of the border sensing information and assist the border managementdepartments in improving the ability and efficiency of border control.国家自然科学基金(61640016)； 广西高校智能计算重点实验室项目(15CI08Y)； 广西维稳研究项目(20161A005