改良分子信标实时PCR快速检测大肠杆菌O_(157):H_7

目的建立改良分子信标实时PCR快速检测大肠杆菌O157H7的快速方法。方法根据GenBank公布O157H7的rfbE保守序列,设计一对引物和改良分子信标探针,用FAM荧光剂标记探针的5’端,建立改良分子信标实时PCR检测O157H7的反应体系,应用于食物中毒快速诊断和食品微生物检测。结果共检测11种细菌,只有大肠杆菌O157H7有荧光信号,其余10种全无荧光信号,且与其他细菌无交叉反应,DNA灵敏度为64fg,菌液灵敏度为59cfu/ml或2cfu/PCR反应体系。应用改良分子信标实时PCR反应体系检测10株O157H7均出现特异的荧光信号,无干扰。对89份食品样品进行检测,5份O157H7实时PCR阳性,其余样品为阴性,检测仅需2h。5份阳性样本,经传统方法培养,有3份检出大肠杆菌O157H7。结论改良分子信标实时PCR检测体系快速、灵敏度高,特异性强,可用于大肠杆菌O157H7食物中毒的快速诊断和食品微生物检测,为食源性疾病的分子流行病学调查提供新的检测手段

改良分子信标-实时PCR快速检测志贺菌

目的:建立改良分子信标-实时PCR检测志贺菌的快速方法,应用于志贺菌食物中毒的快速诊断和门诊肠道致病菌的检测。方法:根据GenBank公布志贺菌ipaH基因的保守序列,设计引物和改良分子信标探针,建立实时PCR-改良分子信标检测体系,应用于对志贺菌食物中毒的快速诊断和门诊肠道致病菌的检测。结果:改良分子信标-实时PCR反应体系DNA灵敏度为93 fgμ/l,菌液灵敏度为64 cfu/m l或2 cfu/PCR反应体系,无交叉反应。此反应体系检测67株志贺菌,均出现特异的荧光信号。对细菌性食物中毒样本等共657份样品进行志贺菌检测,42份志贺菌实时PCR阳性,其中41份志贺菌细菌培养阳性。从样品处理到检测结果仅需2 h~1 d时间。结论:改良分子信标-实时PCR检测体系快速、灵敏度高,特异性强,可用于志贺菌食物中毒的快速诊断,为食源性疾病的分子流行病学调查提供新的检测手段,对于提高肠道门诊的工作效率有重要意义

research on geo satellite mobile communications rlc layer retransmission mechanism

为解决卫星链路上的大延迟环境对GEO移动通信协议RLC层应用现有选择重传机制(SR-ARQ)造成吞吐量低下的问题,提出了适用于GEO卫星移动通信协议RLC层的HARQ重传机制(G-HARQ),并在QualNet平台上对GEO卫星移动通信协议RLC层G-HARQ和SR-ARQ吞吐量进行仿真实验。仿真结果表明,G-HARQ重传机制可以减少RLC确认模式下为恢复错误数据包而传送的ACK/NACK消息数量,从而降低在GEO卫星移动通信协议RLC层端到端的总延迟,提高RLC层吞吐量。国家预研基金项目(6150103)To solve the low throughput problem in radio link control (RLC) layer caused by large delay in GEO mobile satellite communication when using existing retransmission mechanism (SR-ARQ), a HARQ schema applied to GEO mobile satellite communication (G-HARQ) in RLC layer is proposed. Then, the G-HARQ and SR-ARQ mechanism of RLC layer is simulated in GEO mobile commutation platform based on QualNet. Simulation result shows that the G-HARQ retransmission mechanism can reduce the number of ACK/NACK control message generated when recover error RLC-Block in RLC acknowledge mode, which can ameliorate the pear-to-pear delay in RLC layer of GEO mobile communication protocol, and improve the system throughput

design and performance analysis of geo telecommunication system llc protocol

针对GEO链路延时大、误码率高的特点,提出基于位图的RBM-ARQ可靠传输算法.对GMPRS逻辑链路控制(LLC)层的可靠传输算法进行改进,采用保持更新终端状态的方法,以加强信道损伤的恢复能力.仿真实验结果表明,合理设置重传时间和重传计数可以提高LLC层的吞吐量、减少延迟,与传统的GMPRS LLC层可靠传输算法相比,RBM-ARQ算法能获得更好的传输性能.国家共性软件预研基金资助项目(6150103)This paper enhances the reliability of the data communication mechanism in GMPRS Logical Link Control(LLC) protocol,to address the issues of long delay and high bit error rates over GEO link.It proposes RBM-ARQ algorithm based on bitmap for reliable data communication.RBM-ARQ enhances the recovery of channel impairment by keeping the terminals updating the buffer status.Simulation results show when retransmission time and count are set properly,LLC layer throughput gets better and delay becomes smaller,and compared with GMPRS LLC layer acknowledged data transfer,RBM-ARQ algorithm achieves better performance and the better recovery of data

基于低轨卫星网络的按需局部拓扑路由算法

提出了一种简单有效的路由算法——按需局部拓扑路由算法。该算法在保证建立的路由具有最小延时的同时,具有很好的收敛时间,能够有效地适应低轨卫星网络的动态拓扑特性。并给出局部拓扑路由算法的仿真结果及分析;最后提出了下一步研究的重点——基于Qos的路由算法

SaclTCP:基于跨层设计的卫星网络传输协议

卫星网络大时延、高误码率及链路不对称等特点,使得TCP/IP协议无法提供令人满意的服务。针对卫星网络的特点,设计了SaclTCP协议,采用跨层设计思想减少层次网络的冗余性,实时获取网络状态信息。协议根据链路层反馈的有效带宽信息准确地设定拥塞窗口门限阈值;在网络层对路由器缓冲队列进行管理,计算网络发生拥塞的概率并发送显示拥塞通知反馈给发送方传输层;还可区分丢包原因,避免由于传输错误造成的发送窗口减小。实验结果表明与传统协议相比,极大提高了传输性能。Satellite network has some special characteristics, such as the big propagation delay, high bit-error rate and asymmetric channels, and these characteristics make TCP/IP protocols incapable of providing satisfying service for satellite network. The idea of cross-layer design can reduce the redundancy of multi-layer, and capture network status information at any moment. The protocol can set a window congestion gate-limit threshold more effectively by getting the available bandwidth information from the link layer. And in the link layer a router buffer queue managing mechanism is set to compute the network congestion probability and then feedback it to the sender. It can also differentiate the packet losses between congestion and link error to avoid reducing the send window unnecessarily. The protocol will use this information to regulate the size of window dynamically. The experiment shows the protocol greatly improved the transport performance of satellite networks