一种检测蓝藻质粒的定量PCR试剂盒及应用

本发明公开了一种检测蓝藻质粒的定量PCR试剂盒及应用，普通PCR反应液I包含针对聚球藻anl50基因设计的一对特异性引物，普通PCR反应液II包含针对聚球藻CDS：ABB57481.1基因设计的一对特异性引物，定量PCR反应液I包含针对聚球藻anl50基因设计的一对特异性引物，定量PCR反应液II中包含针对聚球藻CDS：ABB57481.1基因设计的一对特异性引物，其中扩增anl50基因的普通PCR上游引物与该基因的结合位置在其定量PCR上游引物与该基因结合位置的上游，anl50基因的普通PCR下游引物与该基因的结合位置在其定量PCR下游引物与该基因结合位置的下游。该试剂盒快速检测蓝藻质粒的拷贝数，具有高灵敏度、特异性、稳定性和重现性。适用于蓝藻质粒DNA的定量检测，对于检测蓝藻细胞的DNA损伤具有实际的应用价值。</p

柱前衍生化高效液相色谱法测定2-去氧葡萄糖的含量

目的建立柱前衍生化高效液相色谱法测定2-去氧葡萄糖(2-DG)含量的方法。方法采用1-苯基-3-甲基-5-吡唑酮(PMP)为柱前衍生化试剂,将2-DG在碱性条件下衍生化后直接进样测定。分离柱为HypersilODS2色谱柱(4.6 mm×250 mm,5μm),流动相为100 mmol·L-1醋酸铵缓冲液(pH 5.5)-乙腈(78∶22),流速1.0 mL·min-1,波长249 nm。结果 2-DG在19.68~393.6μg·mL-1浓度范围内与峰面积具有良好的线性关系(r=0.9997);其定量限(S/N=10)和最低检出限(S/N=3)分别为7.8和3.1 ng;平均回收率为101.21%,RSD 0.63%。结论该方法简便实用、检测灵敏度高、测定结果准确,适用于2-DG的质量控制

一种中间格网可组合调换式水生植物共培养装置

本实用新型公开了一种中间格网可组合调换式水生植物共培养装置，玻璃凸条用玻璃胶固定在矩形共培养玻璃缸的第一长侧面、第二长侧面的中间位置，共培养框架两侧的凹槽尺寸与玻璃凸条大小相匹配，共培养格网可根据实验需要选择单面或双面覆于共培养格网框架的正反两个框面，四个角落均用铆钉插于共培养框架的四个铆钉孔中来固定格网，固定好共培养格网的共培养框架对准玻璃凸条插入。该共培养装置结构简单，操作方便，成本低廉，比传统共培养玻璃缸具有更高利用率和研究效果。实验结束后的格网能方便的从框架上取下，清洗、晾干、消毒后能重复利用，大幅度减小了实验误差，提高了实验效率以及实验的准确率、精确度。</p

一种利用荧光检测微囊藻细胞DNA损伤的方法

本发明公开一种利用荧光检测微囊藻细胞DNA损伤的方法，其步骤：（a）将待检测样品和对照样品均分别平均分成三小组；（b）去除细胞角质鞘：离心收集样品中藻细胞并重悬浮于SE缓冲液中洗涤；（c）细胞裂解：将细胞分别重悬浮于Lysis裂解液，加入蛋白酶K和十二烷基磺酸钠，使细胞裂解；（d）细胞DNA链解旋：改变pH，使T、P和B样品在不同条件下解旋；（e）染色：分别向以上T、P和B样品中加入Hoechest 33258染色；（f）荧光测定：离心后于荧光检测器中检测上清液的荧光强度；（g）结果计算：根据待测样品和对照样品中T、B和P样品的荧光来计算DNA链的断裂水平。本方法易于掌握，并且灵敏度高，DNA链上单个断裂位点即可检测到。</p

Lexical Frequency Rank Difference Distributions Between Texts

该文在已有关于"频级""频序"研究的基础上,结合两种不同类型的语料,采用词汇计量分析方法,考察词语的"序差"所具有的分布特点。该研究发现,对于两; 种文本的共有词集,词的序差呈对称分布,且集中分布于中位数附近,存在离群值序差。这一特点在序差图上表现为"中段平直,双尾翘曲"的"双尾分布"形态。; 根据词语序差的分布规律,可以将文本共有词划分为"中段""下尾""上尾"三个层次。"中段"词语反映两个文本的共性特征,"下尾"及"上尾"词语反映两; 个文本的差异性特征,这些特征具有反映文本的主题内容和文体风格的语言学意义。Based on previous studies on frequency and frequency rank of words,this; paper focuses on the analysis of the frequency rank difference(FRD)from; the perspective of lexical quantitative analysis.This paper reveals that; for the common words between texts,the FRDs are distributed; symmetrically and gathered around the median.This characteristic assumes; a"two-tailed distribution",which is flat in the middle and curving in; both ends.Three lexical levels,i.e.middle,downward end and upward; end,are summarized based on the FRD distributions.The middle lexicon; reflects the common characteristics of the two texts,while the lexicon; that belongs to both ends reflects their own distinctive features.These; features are of linguistic significance in reflecting the thematic; content and stylistic features of the texts.香港教育大学(Internal Research Grant

Liquid and solids charging : charge excess and surface properties effect

Orientador: Fernando GalembeckTese (doutorado) - Universidade Estadual de Campinas, Instituto de QuímicaResumo: Nesta tese, investigou-se a eletrização e os mecanismos de acúmulo de carga em gotas de água e filmes de polietileno, bem como, avaliou-se o efeito das cargas elétricas em algumas propriedades desses materiais. A eletrização de água foi realizada de duas formas: pelo contato com uma superfície metálica polarizada ou expondo as gotas de água a um potencial eletrostático criado através do ar por um eletrodo sem contato. Em ambos os casos, observou-se que a água adquire excesso de cargas e que o valor detectado excede o limite de Rayleigh em algumas dezenas. Verificou-se que a presença de cargas em gotas de água leva à diminuição de sua tensão superficial e ao aumento espontâneo de sua área, por outro lado, não foi observada mudança na sua densidade. O reconhecimento desses fatos deve contribuir para uma maior compreensão de vários fenômenos eletrostáticos que ocorrem na presença de água em estado líquido ou adsorvida. A superfície superior e inferior de filmes de polietileno expostos à descarga corona adquire potencias elevados e opostos formando um filme dipolar. No entanto, a molhabilidade, a composição química e a rugosidade das duas superfícies são bastante diferentes. Enquanto a superfície superior é modificada pelo tratamento corona, a superfície inferior, apesar de se tornar eletrizada, permanece inalterada após o tratamento. Estes resultados mostram que a eletrização da superfície voltada para o eletrodo corona ocorre por um processo diferente da superfície oposta. A eletrização da superfície inferior durante o processo corona abre possibilidades para aplicações onde seja desejável ter um filme eletrizado, porém sem modificar as características originais da superfícieAbstract: In this thesis, we investigated charging process and the mechanism of charge build-up in water droplets and polyethylene films as well. We evaluated the effect of electrical charges on some properties of these materials. Charging of water drops was done using two different methods by: contacting an electrified metal or exposing water to the electrostatic potential created through air by a noncontacting biased electrode. In both cases, water drops acquire net electric charge and its value exceeds the Rayleigh limit in a few tens. It was verified that the presence of charges in water droplets leads to reduced surface tension and increased spontaneous its area, but there was no change in density. Recognition of these facts should contribute to further understanding of various electrostatic phenomena taking place in the presence of liquid or adsorbed water. Top and bottom surfaces of polyethylene (PE) films exposed to corona discharge display large and opposite electrostatic potentials, forming an electric bilayer. However, water wetting, chemical composition and roughness of the two surfaces are quite different. While the top surface is modified by corona treatment, the bottom surface is charged but remains unchanged after treatment. These results show that poling the surface closer to the corona electrode triggers another but different charge build-up process at the opposite surface. Charging of the bottom surface during corona opens possibilities for applications where it is desirable to have a polymer film charged, but without modifying the original characteristics of the surfaceDoutoradoFísico-QuímicaDoutora em Ciência

不同氮、磷浓度对穗花狐尾藻生长及酚类物质含量的影响

为揭示解营养物质对穗花狐尾藻生长及其体内酚类物质的影响,研究了不同氮、磷浓度下穗花狐尾藻体内可溶性糖、可溶性蛋白和总酚含量的变化及与酚类代谢相关酶——苯丙氨酸裂解酶(Pheny lalanine ammonia-lyase,PAL)的活性.结果表明,不同氮、磷条件下,穗花狐尾藻顶端组织中可溶性蛋白含量没有明显变化,低氮、低磷水平下顶端组织中可溶性糖含量分别为50.91、38.25mg.g-1(以干重计),均高于对照组,表明穗花狐尾藻体内可溶性糖对氮、磷胁迫的响应较可溶性蛋白敏感.不同氮浓度(0.875、7.0、56.0mg.L-1)和磷浓度(0.194、1.55、12.4mg.L-1)下,穗花狐尾藻顶端组织中总酚含量分别为44.74、24.42、29.73mg.g-1和37.77、30.60、36.05mg.g-1(以干重计),对应PAL酶活性的变化趋势与酚类物质变化趋势一致,而生物量增长率与酚类物质变化趋势相反,即过高和过低的氮、磷胁迫均会导致穗花狐尾藻生物量增长率的降低及体内酚类物质含量的升高

基于化感物质释放特性的沉水植物抑藻作用模式研究进展

沉水植物对藻类的化感抑制作用,是沉水植物获取竞争优势和维持清水稳态的重要机制之一。化感物质是由植物产生并释放到水环境中的次生代谢产物,化感物质的有效释放和作用是实现沉水植物化感抑藻作用的关键环节。因此,在化感物质释放水平阐明沉水植物化感抑藻的作用模式、过程和机制具有重要意义。通过比较沉水植物化感物质释放到水环境中的种类、含量和常规急性毒性测试中化感物质的抑藻效果,发现沉水植物化感物质在释放水平上的作用模式不同于常规急性毒性试验中的单次作用。为了回答沉水植物化感物质在释放水平如何高效抑藻的问题,结合化感物质的释放特性,重点从化感物质的联合作用和持续作用等角度探讨沉水植物化感抑藻的作用模式,提出沉水植物可能通过多种化感物质低剂量持续释放的方式,实现对目标藻类的持续协同控制。今后有必要进一步结合沉水植物与目标藻类的共存系统与原位实验,借助分析化学、植物化学、细胞和分子生物学的技术手段,从生态学水平加强沉水植物化感抑藻作用机制研究

浮游植物中一氧化氮的生理作用研究进展

一氧化氮（NO）作为一种具有生物活性的气体自由基分子，它的功能代表了生物学系统中信号传递的新途径。大量证据表明， NO在浮游植物细胞中的功能和在高等动植物中类似，具有调节生长和参与抗逆性的作用， NO和ROS可能作为信号分子参与介导浮游植物程序性死亡（PCD）过程。文章较全面地介绍了NO在浮游植物中的产生途径、测定方法、生理功能和PCD的关系及作为信号分子的作用，并对该领域今后的研究进行了展望

丝状绿藻生长的环境影响因子及控制技术研究进展

丝状绿藻是水生态系统中一类重要的初级生产者,由于其与沉水植物、浮游微藻的生态位高度重叠,所以这三者之间存在着复杂的相互作用关系。近年来,在成功恢复沉水植物的清澈湖泊中出现了大量繁殖的丝状绿藻,过度生长的丝状绿藻会影响沉水植物的生长、扩繁和浮游微藻的群落动态变化。因此,如何控制丝状绿藻的过度增殖成为当前水生生态系统研究的关注焦点。本文从丝状绿藻的角度出发,综述了丝状绿藻生态学功能、生长影响因素; 过度增殖的控制方法及其与沉水植物、浮游微藻之间的生态学竞争优势的研究进展,并从丝状绿藻的生活史、竞争机制等方面展望了丝状绿藻的控制策略,以期为水生态恢复和湖泊生态管理提供理论依据