Screening, Identifying of Cellulose-Decomposing Strain L-06 and Its Enzyme-Producing Conditions

从用于堆肥的水稻秸秆中初筛出一株高效纤维素降解菌L-06,根据18SrRNA基因序列和菌株形态分析,初步鉴定该菌为斜卧青霉(Penicillium decumbens)。研究了液体发酵培养基中氮源、碳源、表面活性剂、培养温度、起始pH以及接种量对该菌株各纤维素酶活力的影响。在最适条件下,该菌的β-葡萄糖苷酶(BGL)、外切纤维素酶(CBH)于培养第3天酶活力分别达到1662u/mL和2770u/mL;内切纤维素酶(EG)、滤纸糖化力(FPase)于培养第4天酶活力分别达到18064u/mL和4035u/mL。在产酶优化实验中,该菌的EG和CBH在pH10的培养条件下分别保持了70%和43%的酶活性;在50oC培养条件下EG和CBH分别保持了68%和59%的酶活性。表现出了耐热,耐碱的特性。Cellulases are relatively costly enzymes that are sold in large volumes for use in different industrial applications, and a significant reduction in cost will be important for their commercial use in biorefineries. The production of cellulase is a major factor in the hydrolysis of cellulosic materials. Hence it is essential to make the process economically viable. A strain (L-06) with high cellulase activity was screened from rice straw compost and classified as Penicillium decumbens by the analysis of its morphology and 18S rRNA gene sequences. Different conditions of liquid fermentation medium including nitrogen source, carbon source, surfactant, temperature, initial pH, inoculation quantity for the production of cellulase had been studied. The maximal β-1, 4-glucosidase(BGL) activity was 1662 u/mL which is 1.49 times of the previous and the maximal exo-β-1, 4-glucanases(CBH) activity was 2770 u/mL which is 1.36 times of the previous, cultured in the optimal condition for three days. And the maximal endo-β-1, 4-glucanases (EG) activity was 18064 u/mL which is 1.87 times of the previous and the maximal filter paper enzyme(FPase) activity was 4035 u/mL which is 1.47 times of the previous , cultured in the optimal condition for four days. In the optimization experiments, the EG and CBH in the culture condition (pH10) maintained 70% and 43% activity. In the culture condition(50oC) EG and CBH maintained 59% and 68% activity, which showed heat and alkali resistant characteristics.国家高技术研究与发展项目(No.2006AA05Z111);; 广东省自然科学基金(No.06300998);; 福建省科技平台项目(No.2006H0091)资助~

活心丸(浓缩丸)治疗冠心病稳定性心绞痛的多中心、随机、双盲、安慰剂对照临床研究

目的观察活心丸(浓缩丸)治疗冠心病稳定性心绞痛的临床疗效和安全性。方法本研究采用多中心、随机、双盲、安慰剂对照研究方法,将480例气虚血瘀型冠心病稳定性心绞痛患者以3:1比例随机分为试验组(360例)和对照组(120例)。在西医常规治疗基础上,两组患者分别服用活心丸(浓缩丸)和安慰剂,1次2粒,每日3次;疗程8周。观察两组治疗前后主要指标(心绞痛症状积分)和次要指标(包括硝酸甘油减停率、中医证候积分及生活质量评价)及安全性。结果共454例患者完成试验(试验组336例,对照组118例)。与本组治疗前比较,试验组和对照组心绞痛症状积分和中医证候积分均降低,西雅图心绞痛量表评分改善(P<0.01);与对照组治疗后比较,试验组心绞痛症状积分、中医证候积分、西雅图心绞痛量表评分改善均优于对照组(P<0.01)。试验组心绞痛症状疗效总有效率为80.95%,硝酸甘油停减率为80.70%,中医证候疗效有效率80.65%,均高于对照组[36.44%、45.07%、38.99%(χ~2=58.21、40.94、66.55,P<0.01)]。试验组中既往有心梗史患者心绞痛症状总有效率为83.22%,中医证候有效率83.22%,西雅图心绞痛量表评分为(361.74±62.10)分,均高于无心梗史患者[60.92%、66.89%、(327.95±65.07)分(χ~2=13.89、13.26,P<0.01)]。治疗过程中未发生明显不良反应。结论活心丸(浓缩丸)治疗气虚血瘀型冠心病稳定性心绞痛疗效显著,尤其适用于既往有心梗病史的冠心病稳定性心绞痛患者,无明显不良反应。国家中医药管理局资助课题(No.国中医药科2016ZX04

Fractional Cable Equation by Finite Volume Method

CAblE方程是神经元动力学中最重要的基本方程之一,而用于描述神经纤维活动的分数阶CAblE方程能够更好地模拟神经元的动力学行为.文章采用有限体积法离散得到数值逼近格式,求解一维和二维的分数阶CAblE方程.并用提出的数值方法求解一维和二维情况的两个数值例子,从而说明数值方法的有效性.The cable equation is one of the most fundamental equations for modeling neuronal dynamics.The fractional cable equation has been used to describe nerve fiber.And it has obtained better result in simulating neuron's dynamics behavior.In this paper,we make the first attempt to apply the finite volume method in the solutions of the fractional cable equation in one-dimensional case and two-dimensional case.Firstly the numerical approximation scheme by finite volume method is presented.Then,two numerical examples including one-dimensional case and two-dimensional case are presented by using the numerical technique,which shows the efficiency of the numerical method.国家自然科学基金资助项目(11001090;11026094);福建省自然科学基金资助项目(2010J01011;2010J05009);华侨大学科研启动基金资助项目(08BS507