Characteristics of Polymer Waveguide Sensor Based on Local Surface Plasmon Resonance

以SU-8光刻胶作为波导芯层材料,设计了基于金纳米粒子的局域表面等离子体共振(LSPR)波导传感器。根据Mie理论,建立了金纳米粒子的消光模型,理论分析了纳米粒子半径、待测物折射率等因素对局域表面等离子体共振曲线的影响。分析表明:当待测液体折射率增大时,LSPR共振峰的位置发生红移。随着金纳米粒子半径的逐渐增大,传感器灵敏度增加。共振吸收峰逐渐由单峰变为双峰,其中一个峰位于520 nm波长附近,主要由表面等离子体吸收造成;另一个峰随金纳米粒子半径的增大而逐渐红移,主要由表面等离子体散射造成。A SU-8 polymer waveguide sensor based on local surface plasmonresonance( LSPR) was designed. The extinction model of Au nanoparticles was established. Influence of Au nanoparticle'radius and refractive index of analyte on LSPR curve was analysized. The theoretical analysis results show that the resonance wavelengths of LSPR sensor move to longwave direction when the refractive index of the analyte increases. With the increasing of Au nanoparticles' radius,the sensitivity increases and the resonance absorption peak gradually changes from one peak to two peaks. One of the peaks locates near 520 nm wavelength,mainly caused by surface plasmon absorption. Another peak which is caused by surface plasmon scattering,moves to longwave direction gradually with the increasing of Au nanoparticles' radius.厦门大学校长基金(20720150086);; 国家自然科学基金(61107023);; 教育部博士点专项科研基金(20110121120020)资助项

基于气候分析的城市通风系统构建方法初探——以厦门市为例

在城市气候问题持续恶化与精细化城市管理背景下,对城市通风系统构建方法的辨析与思考将为复杂条件下的城市通风廊道建设提供重要前提。本文基于地理信息汇总与气候分析开展补偿空间与可利用风资源识别,通过卫星遥感数据与气象观测数据耦合进行作用空间识别,运用Rhino、Grasshopper工具与GIS技术,采用最小成本路径分析法获取潜在的城市通风路径,综合考虑系统风、海陆风、逆温的影响,进行通风条件评估与廊道边界界定,进而推导城市通风廊道的形态,获取通风廊道各片段差异化、具体化的设计导则。实证表明,在问题解决与成本控制导向下,以气候分析为基础的城市通风系统构建方法有助于城市通风廊道建设思路优化与技术升级。国家自然科学基金资助项目（51408516）;;\n福建省自然科学基金计划资助项目（2019J01007）;;\n厦门市科技计划项目（XJK2017-1-11

The Basic Study on Silicon-Base Polymer Waveguide Surface Plasmon Sensor

表面等离子体共振（SPR）传感器是近年来光电子学传感器领域的研究热点之一。其具有高灵敏、免标记、快速响应等特点，在医学、生物与环境传感等领域有着广阔的应用前景。采用聚合物材料制备表面等离子体共振传感器，只须通过室温旋涂和光刻等工艺，成本低廉，制作的器件轻巧，在平面光子集成应用方面前景十分看好。 本论文对聚合物光波导表面等离子体共振传感器进行了基础研究，分别对基于连续金膜、基于金纳米颗粒的光波导表面等离子体共振传感器进行了理论分析与实验制备，具体工作如下： 1.利用Fresnel公式对基于连续金膜的传导型表面等离子体共振传感器进行了理论分析。研究了金属膜类型、金属膜厚度、芯层折射率、以及待测...Surface Plasmon Resonance Sensor is a hotspot research in the field of optoelectronics sensor. It has extremely prospect in the filed of medical sensing, bio-sensing and environmental sensing due to its distinguishing feature of high sensitivity, label-free and rapid response. Organic polymer material is a common material in daily life, it has the advantages of simple preparation and low cost. Due...学位：工学硕士院系专业：信息科学与技术学院_微电子学与固体电子学学号：2312013115310

竞争型全光增益控制放大器仿真研究

针对信道数变化等原因造成的剩余信道输出增益漂移现象,研究了一种竞争型全光增益控制放大器,可以实现对宽输入功率范围信号的增益控制。对光功率在-40～5 dBm范围内的输入光,将增益漂移量由22.0 dB降低至0.4 dB。在多信道情况下,将信道数变化和信道功率变化引起的增益漂移量分别降低至0.23和0.10 dB。该放大器的工作范围与控制幅度都要优于传统的全光增益方法,对于控制剩余信道输出增益稳定具有重要参考价值

Screening of marine bacterium producing carrageenase and its enzymatic properties

从病烂的海洋植物中分离到一株高产卡拉胶酶的海洋细菌YK-5,革兰阴性,初步鉴定为黄杆菌属(Flavobacterium)中的一个种。该菌株在温度28℃,培养基起始pH8.5条件下培养48h产生碱性卡拉胶酶活力高达149.8U/mL。酶学性质的初步研究显示,YK-5产生的卡拉胶酶最适反应pH值为9.0,在pH8.0~11.0范围内具有较高的酶活性和较好的稳定性;最适反应温度为30℃,且在40℃以下具有较高的温度稳定性。经传代培养证实该菌株遗传性能稳定。

The analysis of the genotyping of plasmid-mediated AmpCβ-lactamases produced by clinical strains of Escherichia coli and Klebsiella pneumoniae

目的针对该院临床分离大肠埃希菌和肺炎克雷伯菌质粒型AMPC酶基因型进行研究分析。方法收集2011年7月至2012年8月对头孢西丁不敏感无重复临床分离大肠埃希菌和肺炎克雷伯菌共176株,采用聚合酶链反应(PCr)法和扩增全基因序列分析大肠埃希菌和肺炎克雷伯菌产AMPC酶基因型。结果 PCr结果显示,AMPC酶基因(AMPC基因)阳性率为18.2%主要以dHA型为主,阳性率为59.4%,CIT型为37.5%,EbC为3.1%;其中,大肠埃希菌AMPC基因阳性率为11.4%,以CIT型为主,阳性率为77.8%,dHA型和EbC型阳性率均为11.1%;肺炎克雷伯菌AMPC基因阳性率为23.7%,以dHA型为主,阳性率为78.3%,CIT型为21.7%。基因序列结果显示,dHA型有18株为dHA-1基因型和1株摩根摩根菌AMPC基因型,一致率97.0%,CIT型有10株为CMy-2基因型,1株CMy-42基因型和1株CMy-4基因型;EbC型为阴沟肠杆菌AMPC基因型,一致率为99.0%。将32株基因序列提交gEnbAnk,均被接受,其登录号为kJ127248~kJ127279。结论该院临床分离大肠埃希菌AMPC基因主要以CMy-2型为主,而肺炎克雷伯菌主要以dHA-1型为主。Objective To investigate the genotype and epidemiology of plasmid-mediated AmpCβ-lactamases produced by the clinical strains of Escherichia coli and Klebsiella pneumoniae.Methods A total of 176 clinical nonrepetitive cefoxitin non-sensitivity isolates of Escherichia coli and Klebsiella pneumoniae was collected from July 2011 to August 2012.Polymerase chain reaction(PCR)for AmpC enzyme gene amplification and DNA sequencing were carried out for genotype of AmpC beta-lactamases.Results The results of PCR showed that the positive rate of ampCof the 176 strains of Escherichia coli and Klebsiella pneumoniae AmpC was 18.2%,mainly DHA type,counting for 59.4%,CIT counting for 37.5%,EBC counting for 3.1%.The positive rate of ampC of Escherichia coli was 11.4%,mainly CIT type,counting for 77.8%,the positive rates of DHA type and EBC type both were11.1%.The positive rate of ampCof Klebsiella pneumoniae were 23.7%,mainly DHA type,counting for 78.3%,CIT type counting for 21.7%.The results of DNA sequencing showed that there were 18 strains DHA-1type and 1strain ampCgene type of Morganella morganii in DHA type strains,the concordance rate was 97.0%,10 CIT type strains was CMY-2type,1strain was CMY-42,one strain was CMY-4type,EBC type was ampCgene type of Enterobacter cloacae,the concordance rate was 99.0%.A total of 32 strains of gene sequencing were registered as KJ127248-KJ127279 in GenBank.Conclusion The main genotypes of plasmid-mediated ampCenzyme produced by Escherichia coli and Klebsiella pneumoniae were CMY-2and DHA-1respectively.福建省自然科学基金项目(2013D002); 福建省卫生厅青年基金项目(2010-2-90

Clinical value of fluorescent PCR-probe melting analysis in detection of plasmid-mediated AmpC β-lactamase genes

目的探讨多重荧光PCR-探针熔解分析法应用于临床分离大肠埃希菌和肺炎克雷伯菌中ampC耐药基因的检测方法,并评价该方法的临床应用价值。方法收集医院2009年7月-2010年6月的临床分离菌株,首先采用头孢西丁纸片法进行耐药表型筛选,再同时应用传统PCR技术与荧光PCR-探针熔解曲线法对临床分离菌株进行检测,并对ampC耐药基因扩增产物进行DNA测序。结果经头孢西丁纸片法筛选出176株对头孢西丁不敏感临床分离菌株,其中97株为肺炎克雷伯菌、79株为大肠埃希菌;在176株临床分离株中,荧光PCR-探针熔解分析法检测出36株ampC耐药基因阳性菌株,包括18株DHA型、12株CIT型和5株EBC型,还有1株肺炎克雷伯菌同时含有DHA型和EBC型;而传统PCR技术检出32株ampC耐药基因阳性,两个检测结果的符合率为97.7%;DNA测序后经BLAST比对,荧光PCR-探针熔解分析法检测ampC基因型与所检测目的基因型一致。结论荧光PCR-探针熔解分析法能高效检测出质粒介导ampC耐药基因,其方法简便、敏感性高、特异性强,具有良好的临床应用价值。OBJECTIVE To explore the clinical value of multiplex real-time PCR-probe melting analysis in detection of ampC drug resistance gene in clinical isolates of Escherichia coli and Klebsiella pneumoniae.METHODS The clinical isolates were collected from Jul 2009 to Jun 2010;the drug resistance phenotypes were screened by using cefoxitin disk agar diffusion method,the clinical isolates were detected by means of the traditional PCR technique and fluorescent PCR-probe melting curve method,and the DNA sequencing was performed for the amplified products of the ampC drug resistance gene.RESULTS Totally 176 clinical isolates that were not sensitive to cefoxitin were screened out by using cefoxitin disk method,including 97 K.pneumoniaeisolates and 79 E.coli isolates.Of the 176 clinical isolates,36 were detected positive for the ampC drug resistance gene by using the fluorescent PCR-probe melting analysis method,including 18 strains positive for DHA,12 strains positive for CIT,5strains positive for EBC,and 1strain positive for both DHAand EBC;while 32 strains were detected positive for the ampC drug resistance gene by the traditional PCR technique,the coincidence rate of the two detection results was97.7%;the DNA sequencing analysis showed that by contrast to BLAST,the ampCdrug resistance gene detected by the fluorescent PCR-probe melting analysis method was consistent with the target genotype.CONCLUSIONThe fluorescent PCR-probe melting analysis method can effectively detect the plasmid-mediated ampCdrug resistance gene,it is simple,highly sensitive and specific and worthy to be promoted in hospitals.国家自然科学基金资助项目(81000762);; 福建省自然科学基金资助项目(2013D002);; 福建省卫生厅青年基金资助项目(2010-2-90);; 厦门科技局基金资助项目(3502z20089003

基于解耦并联机构的核主泵螺栓检测环形平台粗精两级快速调整

环形平台作为核主泵螺栓在线检测的移动和操作基准，其位姿快速、高精度调整是螺栓在线精确检测的关键。针对辐射环境环形平台位姿调整需求，提出了基于解耦并联机构的粗、精两级高精度快速调整方法，第一级粗调以6自由度解耦并联机构对环形平台进行初步调整，第二级精调以两独立方位特征子集对应的1T2R和2T1R解耦并联机构对平台按序进行水平度和同轴度调整；基于方位特征集理论，综合了粗、精两级调整解耦并联机构；提出了以海绵吸具在泵体弧形光滑外壁吸附形成固定支点，构造并联机构定平台，实现了各并联机构的快速安装、切换及拆卸。基于解耦并联机构的粗精两级快速调整，为实现核辐射环境环形平台的快速高精度调整提供了新思路

新型3T1R力触觉主端操作并联机构设计与分析

具有力触觉反馈主从操作的检测装置是提升核主泵螺栓在线检测准确率、避免核辐射健康损害的有效手段。针对核主泵中空螺栓检测超声探头三平移一转动的深孔运动特征，基于方位特征集并联机构拓扑综合方法，构造了一种新型4-HSOC{-Ri1//Ri2(-P(4R))//Ri3⊥Ri4-}力触觉主端并联操作机构；以矢量方程法求解了该机构正逆运动学，并进行了工作空间、运动特性分析和仿真；对应从端深孔检测作业特征进行了工作空间匹配分析和实验验证。该主端力触觉并联机构具有对称折叠性和良好的动平台转动能力，适用于竖向深孔扫查作业，契合从端核主泵螺栓检测装置检测需求，为核主泵螺栓检测装置对孔、扫查任务及主从端运动匹配提供了理论基础