银鲫两个蛋白合成相关基因全长cDNA的克隆及其特征分析

通过构建雌核发育银鲫心跳期SMARTcDNA质粒文库并从文库中随机挑选克隆测序 ,克隆得到银鲫翻译起始因子 3亚单位 2 (GTIF3 S2 )和翻译延伸因子 1亚单位α(GEF 1α)基因全长cDNA。银鲫翻译起始因子 3亚单位 2基因cDNA全长 12 80bp ,开放阅读框位于 117— 10 91bp之间 ,编码 32 5个氨基酸。其推断的氨基酸序列存在三个WD结构域。该基因在鱼类中为首次报道。银鲫翻译延伸因子 1亚单位alpha基因cDNA全长 1784bp ,开放阅读框位于82— 14 6

River eco-regionalization oriented by ecological restoration

经济社会的快速发展不可避免的对河流生态系统造成了损伤,如何使受损河流生态系统恢复到健康状态已经成为当前亟待解决的重要环境问题之一.河流修复生态功能分区是对河流进行适应性生态修复的必要前提和基础,可为制定生态修复目标提供科学依据.本研究在已有分区体系基础上,依据生态功能分区的理论,对河流修复生态功能分区的内涵进行了辨析,从理论上对分区方法进行了探讨,并以永定河(北京段)为例进行了实证研究,提出了永定河(北京段)河流修复生态功能分区方案,最终结果划分6个区域;并结合区域社会经济发展状况和相关发展规划等因素,提出各区段的生态修复调控指标,为永定河(北京段)生态修复提供科学指导.Rapid socio-economic development would inevitably result in the damage of the river ecosystem at present and how to restore damaged river ecosystems to health status has become one of the important environmental issues urgent to be solved.Function zoning is the prerequisite for adaptive restoration,which could provide the scientific basis for the ecological restoration goal.In this work,function zoning of river was firstly compared with the main ecological regionalization,for both advantages and shortcomings.Based on the theories of ecological function regionalization,the conception of river restoration and eco-regionalization was discussed and the approach of eco-regionalization was studied theoretically.The Yongding River(Beijing segment) was selected as a study case and ecological function zoning including six river reaches was developed.The regulation index for ecological restoration of each reach was also proposed,which focused on ecological restoration of Yongding river in Beijing.北京市科学技术委员会"永定河生态修复目标体系研究"项目(No.D090409004009003);国家自然科学基金(No.41001108);山东省自然科学基金(No.ZR2009DL011);北京市生态学重点学科项目---

Nutrient requirements and development of formulated diets of mud crab,Scylla sp.

【中文摘要】 青蟹属(Scylla)有锯缘青蟹(Scylla serrata),拟穴青蟹(S.paramamosain),紫螯青蟹(S.tranquebarica)和榄绿青蟹(S.olivacea)等4个种。我国以拟穴青蟹占绝对优势,它有个体大、生长快、适应性强、肉味鲜美、营养丰富等特点,已成为具有重要经济价值的海水养殖蟹类之一。随着青蟹人工育苗技术的突破、养殖技术的改进和多种养蟹形式的开发,养蟹业得到了蓬勃发展,其养殖规模不断扩大,产量不断提高,据统计,2004年全国青蟹养殖产量已达10.85万吨。养蟹产业的形成和持续发展必然要走健康养殖的道路,而健康养殖除了供应高质量苗种和保持良好的养殖环境外,必须供应量足质优的饲料,以满足青蟹的营养需求。然而,尽管我国青蟹养殖已有100多年的历史,但对其营养需求研究和配合饲料研发起步较晚,国外的相关研究也较少,故目前市场上尚未生产出能满足其正常生长发育所需营养需求的配合饲料,养蟹所用的饲料主要是天然低质鱼、虾、贝类等。鲜杂鱼等天然生物饵料资源有限,供应不稳定,使用不方便(需要冷冻保存),营养不全面,自身携带病原菌,且投喂后容易腐烂分解,败坏水质,易导致病害蔓延,难以满足日益扩大的养蟹... 【英文摘要】 The mud crab,Scylla sp.,is an important crustacean species farmed on a commercial scale in China.They are polycultured with fish or shrimp,but mud crabs are now monocultured in increasing density to supply the growing market.This trend has made diet development more important.Information on the nutrients required for growth of mud crabs is vital for the formulation of an effective feed.This paper reviews the current knowledge on Scylla sp.nutrition with special reference to developments during the last deca...国家“八六三”计划项目(2002AA603013); 厦门大学高层次人才引进科研启动项目; 厦门大学“九八五”计划科技创新项目“青蟹亲体脂肪酸营养与幼体发育关系研究

Photo-generated dinuclear {Eu(II)}2 active sites for selective CO2 reduction in a photosensitizing metal-organic framework

利用太阳光驱动CO2还原不仅可降低大气中CO2浓度，而且可以生成重要的化工原料，是缓解气候变暖和开发新能源的理想途径之一。然而如何制备高效光催化剂将CO2高选择性地转化为高附加值的化学产品极具挑战性。课题组巧妙地将具有催化活性的稀土簇合物与光敏配体组装在一起，合成了具有大孔结构的稀土Eu-Ru(phen)3-MOF催化剂，实现了可见光驱动的CO2到甲酸盐的高效选择性转化。研究表明，该材料光催化生成甲酸盐的反应速率可达到321.9 μmol h−1 mmolMOF−1，这是至今为止报道的光催化还原CO2转化为甲酸盐活性最高的MOFs催化剂。 该项研究工作由博士生闫志浩完成，博士生杜明浩参与部分表征工作；中国科学院大连化学物理研究所金盛烨研究员、刘俊学博士在瞬态光谱表征中给予了支持；我校化学化工学院汪骋教授在数据分析过程中给予大量帮助；理论计算由浙江工业大学庄桂林副教授完成。【Abstract】Photocatalytic reduction of CO2 is a promising approach to achieve solar-to-chemical energy conversion. However, traditional catalysts usually suffer from low efficiency, poor stability, and selectivity. Here we demonstrate that a large porous and stable metal-organic framework featuring dinuclear Eu(Ⅲ)2 clusters as connecting nodes and Ru(phen)3 -derived ligands as linkers is constructed to catalyze visible-light-driven CO2 reduction. Photo-excitation of the metalloligands initiates electron injection into the nodes to generate dinuclear {Eu(Ⅱ)} 2 active sites, which can selectively reduce CO2 to formate in a two-electron process with a remarkable rate of 321.9μmolh −1 mmol MOF −1 . The electron transfer from Ru metalloligands to Eu(Ⅲ)2 catalytic centers are studied via transient absorption and theoretical calculations, shedding light on the photocatalytic mechanism. This work highlights opportunities in photo- generation of highly active lanthanide clusters stabilized in MOFs, which not only enables efficient photocatalysis but also facilitates mechanistic investigation of photo-driven charge separation processes.We are grateful for the financial support from the 973 project (Grant 2014CB845601) of the Ministry of Science and Technology of China, the National Natural Science Foundation of China (Grants no. 21422106, 21673184, 21431005, 21721001, and 21390391) and the Fok Ying Tong Education Foundation (151013). We thank Dr. Wen-Ming Qin and the staffs from the BL17B beamline of National Center for Protein Sciences Shanghai at Shanghai Synchrotron Radiation Facility for assistance during data collection; we also thank Miss Ying-Zi Han for helpful measurement on ESI-MS. We also gratefully acknowledge Mr Xuefu Hu for helpful measurement on time-resolved PL and Miss Rong Chen for her assistance on CV measurement and analysis. 研究工作得到科技部（项目批准号：2014CB845601）、国家自然科学基金委（项目批准号：21422106、21673184、21431005、21721001、21390391）、教育部霍英东基金会青年教师基金（项目批准号：151013）等资助

Alternatives to slash & burn: a global strategy: special project for presentation to the global steering group (GSG): first phase January 1993-December 1997

It is estimated that shifting cultivation accounts for about 70 percent of the deforestation in Africa, 50 percent in Asia, and 30 percent in Latin America of the 14 million hectares of tropical moist forests currently destroyed every year. Tropical deforestation is responsible for 18 percent of current global warming, for most of the decimation of plant and animal genetic diversity, and for threatening the stability of many watersheds. Landless farmers from crowded areas migrate to the forested areas and attempt to make a living by slash and burn, which results in unsustainable agriculture and continuing rainforest destruction. Sustainable alternatives to slash and burn would enable millions of poor farmers to make an adequate living without destroying additional forests. Research conducted at several locations for many years shows hope that for every hectare put into promising alternatives, five to ten hectares of tropical rainforest can be spared from the shifting cultivator`s axe every year. Several international centers have decided to join efforts with national research systems (NARS) to formulate a research and development strategy that provides viable alternatives to slash and burn agriculture on a worldwide basis. The strategy focuses on two main targets: 1) reclamation of already deforested lands such as secondary forest fallows and abandoned grasslands and 2) prevention of damages by deforestation itself. The strategy consists of three main components: 1) developing and testing alternative slash-and-burn technologies for small-scale farms, adapted to specific ecoregions within the humid tropics, 2) linking environmentally-oriented strategies with socioeconomic policies that provide incentives for such technologies and disincentives to further deforestation and 3) designing effective economically sound and socially acceptable rainforest conservation methods

银鲫胎球蛋白b基因的克隆及其在胚胎发育过程中的表达图式研究

胎球蛋白是一个包括胎球蛋白-A和胎球蛋白-b的蛋白家族。虽然对于同一蛋白家族的胎球蛋白-a已有详尽报道;证实其是胚胎发育过程中的一个重要蛋白;而且在抑制胰岛素受体酪氨酸激酶活性;抑制蛋白酶抑制剂活性;发育过程中的钙代谢调节以及骨和中枢及外周

A Novel Numerical Analysis Method for Genenalized Ridge Transmission Devices

利用Compact 2-D频域有限差分(FDFD)法分析广义脊形传输器件特性,得到的本征方程只涉及4个横向场分量,与同时处理6个场分量的本征问题相比,大大降低了CPU时间,同时由给定的工作频率f求解相移常数β,易于实际应用。针对广义脊形传输器件的复杂结构,本文划分了不同的子区。几种代表性的脊形波导传输结构分析结果与其他方法一致,表明了本文分析思路的可行性。A compact two-dimensional(2-D) finite-difference frequency-domain method is used to analyze propagation characteristics of generalized ridge waveguides for the first time.Only four transverse field components are involved in the final resulting eigenvalue problem,so the required CPU time is reduced considerably as compared to the approaches by which six field components are comprised.Additionally,the feature of finding the phase constant for a given frequency is more pertinent in practical applications.To deal with the generalized ridge waveguides,we divide complex structure into several regular sub-regions,and apply the compact 2-D FDFD method respectively in every sub-region only by setting boundary conditions accordingly.Finally,the calculated numerical results in the current study show good agreement with the published ones in this field.国家自然科学基金资助项目(10504043

斑马鱼nexilin-like的克隆和表达特征分析

<正>nexilin是一种F-actin结合蛋白。为研究nexilin在胚胎发育过程中的功能;我们克隆了nexilin在斑马鱼中的同源基因nexilin-like。该基因的cDNA全长3360bp;含有一个2586bp的开放阅读框。其编码的氨基酸序列与人类nexilin的同源性有55%。原位杂交显示斑马