沉积物疏浚技术在富营养化湖泊修复中的应用

沉积物疏浚是削减富营养化湖泊内源负荷的最为直接和有效的手段。分析了沉积物疏浚的效果和可能引起的风险;从外源污染、作业区域和深度、沉水植物3个方面探讨了影响沉积物疏浚效果的关键因素。最后指出,富营养化湖泊沉积物疏浚的重要原则是截断外源污染、划分作业区域、合理控制挖掘深度、创造适宜生境、恢复沉水植物、重构生态系统等

Studies on Fatty Acid of White-Spotted Bamboo Shark, Chiloscyllium palgiosum (Bennett, 1830), from Southern Fujian Coast Waters

本论文主要研究闽南近海条纹斑竹鲨(Chiloscylliumpalgiosum)的生物学、肝体指数（LSI）和肝脏脂肪的理化常数、肝脏、软骨和肌肉的含水率、含油率及其脂肪酸组成以及季节变化和个体发育对水分和脂肪组成的影响，同时也比较了不同组织间的成分差异。通过本文的研究，将查清条纹斑竹鲨的软骨、肌肉、肝脏等组织中特定脂肪的含量及其变化，同时还对条纹斑竹鲨各组织内EPA（C20:5）和DHA（C22:6）含量进行分析，为海洋功能性食品和药物研究和开发提供重要参考，具有较高的理论意义；研究结果还将对条纹斑竹鲨资源的利用、保护与管理提供科学依据。主要研究结果如下：1.条纹斑竹鲨的体长（BL）与体重（...Southern Fujian Coast Waters Fishing Ground abundant in aquatic resource. In this thesis, total 100 fish specimens were identified and analyzed. Some data are detailed studies, including biology, the liver-somatic index (LSI), oil content and water content in liver, muscle and cartilage of white-spotted bamboo (C. palgiosum). Total fatty acid composition of liver lipid, muscle lipid and cartilage ...学位：理学博士院系专业：海洋与环境学院海洋学系_海洋生物学学号：B20022701

The Changes of lipid and fatty acid composition in white-spotted bamboo shark ( Chiloscyllium plagiosum) at different growth stages

本文研究了闽南-台湾浅滩渔场的条纹斑竹鲨肌肉和软骨内的脂肪含量和脂肪酸组成，比较了雌雄个体间组成差异、未成熟个体和成熟个体间的组成差异，以及成熟个体在不同时期的差异，由此讨论生殖活动对条纹斑竹鲨肌肉和软骨的脂肪含量和脂肪酸组成含量的影响。 本研究的主要结论有： （1）在条纹斑竹鲨的肝脏和肌肉中，不同时期的脂肪含量不同，未成熟个体内的脂肪含量均低于成熟个体的，不同个体间软骨内的脂肪含量则无显著性变化，含量在2%左右。 （2）在雌雄个体之间，条纹斑竹鲨肌肉和软骨内的脂肪含量均无显著性差异，而在生殖前雌鱼肝脏内的脂肪含量显著高于雄鱼。 （3）生殖活动对雌鱼肝脏内的脂肪含量有极其显著的影响，而...The lipid content and fatty acid composition of muscle and cartilage in white-spotted bamboo shark (Chiloscyllium plagiosum), caught from southern Fujian coastal waters of China, were determined. The concentration and composition differences between male and female, immature and mature, reproductive and after-reproductive stages in mature were studied in detailed to assess the influence of reprodu...学位：博士后院系专业：海洋与环境学院环境科学与工程系_环境科学学号：BH170021

Skin Detection Technology with Fusion Approach

皮肤检测技术发展迅速,并且能够应用于检测和跟踪人体部位、计算机视觉和可视化等多个领域。然而,皮肤检测的主要困难仍然是不同程度的皮肤色调、光照条件和颜色接近肤色的背景等等。本文研究了一种新奇的基于融合策略下的动态皮肤检测,它是由一个平滑动态二维直方图、高斯混合模型和基于脸部皮肤色调颜色计算的实时动态阈值这三种检测方法融合在一起的。本研究通过人脸检测来强化肤色模型,这是因为人脸是不同色调的皮肤颜色的一个突出特征,尤其是在包含不同种族的多个人脸图像中。定性和定量实验结果表明,该方法由于其较低的计算成本和较高的精确度,比目前先进检测技术更稳定有效。The skin detection technology develops rapidly and is capable for a wide range of applications in many fields, such as detect and track the components of human body, computer vision and visualization.However,the principal obstacles faced by skin detection are still the different degrees of skin tone color, illumination conditions and skin color-like backgrounds.This paper proposes a novel skin dynamic detection based on fusion strategy, which fuses a smoothed 2-D histogram, Gaussian model and an online dynamic threshold based on the calculation of face skin tone color.In this research, we adopt face detector to refine the skin model, because face is a prominent indicator of different characteristics of skin tone color, especially in images that include more than one face with different ethnicity.Qualitatively and quantitatively experimental results show that the proposed method is more robust and effective compared to state-of-the-art methods, owing to its low computational costs and high accuracy

The Legal Issues and the Risk Prevention of the Import Documentary Bill

文章结合当前进口押汇的有关理论研究和实务操作中的具体做法,阐述并分析了进口押汇的不同观点,并结合相关法律对银行和开证申请人之间订立的信托收据做进一步论述,最后指出银行和立法机关为防范风险的发生所应该采取的积极措施。This paper analyzes the different viewpoints on the import documentary bill according to the current research and the practical modes of operation.Then,it focuses the discussion on the trust receipt concluded between the banks and the applicants through relating to the relevant laws.At last,the paper indicates that the bank and the legislature must take active measure to prevent the various ventures from happening

少穗竹和四季竹竹笋的营养成分分析

对福建省华安竹种园2种少穗竹属笋用竹少穗竹和四季竹竹笋的基本营养成分进行测定分析。结果表明:四季竹竹笋的含水量、灰分、脂肪含量高于少穗竹,但蛋白质含量低于少穗竹;而少穗竹竹笋氨基酸、必需及半必需氨基酸、呈味氨基酸总含量高于四季竹。厦门市科学技术局科研项目(编号:3502Z20102003和3502Z20144072

Capacity Modulation Device with Changing Concentration of Mixture Refrigerant:A State-of-the-art Review

Air source heat pump is widely used, and a capacity modulation with concentration of mixture refrigerant is a new type modulation of environment protection and energy conservation. In this paper, the state of the art of existing variable concentration devices with mixture refrigerant is presented and their types are described according to their locations in the system. The analysis results indicate that the variable concentration device should be set at the outlet of liquid refrigerant in order to change concentration of mixture refrigerant in air source heat pump. Moreover, a rectification section is more effective to change concentration than a gasliquid separator or liquid reservoir, and the middle throttling apparatus should be an electronic regulation device other than a capillary tube. The improvement and application of variable concentration devices in air source heat pumps are also discussed

5种牡竹属笋用竹竹笋营养成分之比较

对福建省华安县竹种园5种牡竹属笋用竹云南甜龙竹、版纳甜龙竹、勃氏甜龙竹、马来甜龙竹和梁山慈竹竹笋营养进行成分比较。结果表明:5种笋用竹鲜竹笋含水量均接近或超过90%,口感较好;灰分含量勃氏甜龙竹竹笋的最高,达0.893%,版纳甜龙竹最低（0.340%）;蛋白质含量最高的是马来甜龙竹（1.717%）,最低的是版纳甜龙竹（1.260%）;勃氏甜龙竹（0.281%）和云南甜龙竹（0.292%）脂肪含量较低,马来甜龙竹（1.134%）和梁山慈竹（1.083%）脂肪含量较高;勃氏甜龙竹（0.655%）和云南甜龙竹（0.666%）粗纤维含量较低,而梁山慈竹、版纳甜龙竹和马来甜龙竹含量相近,达较高水平,分别达0.953%、0.894%和0.879%。5种竹笋均含有17种氨基酸,氨基酸总含量最高的是勃氏甜龙竹(227.64 mg·g-1),最低的是马来甜龙竹(120.43 mg·g-1);必需半必需氨基酸总含量最高的是勃氏甜龙竹（84.26%）,最低的是马来甜龙竹（50.67%）;呈味氨基酸总量最高的是勃氏甜龙竹,达212.79 mg·g-1,其次依次为梁山慈竹(164.71 mg·g-1)、版纳甜龙竹(156.35 mg·g-1)、云南甜龙竹(141.44 mg·g-1)和马来甜龙竹(115.83 mg·g-1)。厦门市科学技术局科研项目资助(3502Z20102003;3502Z20144072

MiR-127-3p靶向MAPK4对葡萄膜黑色素瘤细胞增殖、凋亡、迁移和侵袭的影响

【目的】探讨miR-127-3p对葡萄膜黑色素瘤细胞增殖、凋亡、迁移和侵袭的作用。【方法】通过RT-qPCR检测人葡萄膜黑色素瘤组织及细胞、正常组织及细胞中miR-127-3p与MAPK4mRNA的表达；通过Lipofectamine2000说明书将mimic-NC、miR-127-3pmimic、pc-MAPK4质粒分别或联合转染进入SP6.5或OM431细胞；通过双荧光素酶报告检测miR-127-3p与MAPK4复染靶向关系；通过CCK-8法检测细胞增殖，流式细胞术检测细胞凋亡，划痕实验检测细胞迁移能力，Transwell法检测细胞侵袭能力，蛋白印迹法检测AKT/mTOR通路蛋白相对表达水平。【结果】在葡萄膜黑色素瘤组织和细胞系中，miR-127-3p表达明显下调（P<0.01），而MAPK4表达明显上调（P<0.01）；miR-127-3p与MAPK43′UTR区存在结合位点，miR-127-3p高表达明显抑制了含有野生型MAPK4质粒的荧光素酶活性（P<0.01），但对突变型MAPK4质粒的荧光素酶活性无影响；与Control组相比，miR-127-3pmimic组SP6.5细胞和OM431细胞增殖均明显下降（P<0.01），凋亡率均明显增加（P<0.01），划痕闭合率均明显降低（P<0.01），每视野侵袭细胞数目均明显减少（P<0.01），p-AKT（T308）/AKT、p-mTORr（S473）/mTOR蛋白表达均明显下调（P<0.01），共转染pc-MAPK4逆转上述变化。【结论】MiR-127-3p通过靶向下调MAPK4来抑制葡萄膜黑色素瘤细胞增殖、迁移和侵袭，诱导细胞凋亡，这可能与抑制AKT/mTOR通路激活有关

Breeding high-yield oil-producing strain to use cheap carbon source by UV induced protoplast mutagenesis

【目的】研究并建立利用原生质体紫外诱变技术选育可利用廉价碳源发酵的高产油新菌株的方法。【方法】采用1.5%蜗牛酶和1.0%纤维素酶混合液水解去除细胞壁得到2A00015(近平滑假丝酵母,CAndIdA PArAPSIlOSIS)的原生质体,将其放于紫外灯下诱变及再生壁培养,筛选获得可利用廉价碳源发酵的高产油酵母,并采用气相色谱质谱联用法(gC-MS)测定其脂肪酸组成。【结果】突变效果最好的突变菌株2A00015/25用葡萄糖发酵培养7 d后,其生物量、油脂产率和产油量分别为17.77 g/l、58.12%和10.32 g/l,较原始菌株分别提高了12.45%、23.32%和38.68%;利用废糖蜜发酵培养,其生物量、油脂产率和产油量分别为18.54 g/l、49.44%和9.17 g/l,较原始菌株分别提高了9.09%、21.16%和32.18%。利用废糖蜜培养其产油效率虽低于利用葡萄糖培养,但从环境保护及原材料成本的角度考虑,用废糖蜜作为碳源发酵培养产生油脂更具优势。诱变菌株利用废糖蜜发酵后产生油脂经检测含有8种脂肪酸,其脂肪酸组成与植物油近似,其中不饱和脂肪酸含量占脂肪酸总量的82.4%。【结论】通过利用原生质体紫外诱变技术,成功选育出一株新的可利用廉价碳源的高产油海洋菌株,产油率达到49.4%,提高了21.2%。[Objective] We used UV induced protoplast mutagenesis to study breed a new high-yielding lipid-producing strain which could use cheap carbon source.[Methods] Get the 1.5% glusulase and 1.0% cellulose solution.Hydrolyze to remove the cell wall and obtain the protoplast of 2A00015(Candida parapsilosis).Put it under the ultraviolet lamp for mutagenesis and cultivate regenrated wall.Then screen to get the high oil generated yeast which could be fermented by low-cost carbon source.Determine its fat acid components by gas chromatography with mass spectrometric(GC-MS).[Results] Cultivated the mutant strain with best mutation 2A00015/25 in the glucose.We found that the biomass, oil yield rate and oil production are separately 17.77 g/L, 58.12% and 10.32 g/L, which are separately 12.45%, 23.32% and 38.68% higher than the original strain.We have also cultivated the mutant strain in the waste molasses and found the biomass, oil yield rate and oil production are separately 18.54 g/L, 49.44% and 9.17 g/L, which are separately 9.09%, 21.16% and 32.18% higher than the original strain.The oil yield rate is lower in the waste molasses cultivation than that in glucose cultivation.However, in consideration of environment protection and cost of raw materials, the waste molasses is of much more advantages.It is tested that the fat generated from the waste molasses fermentation consists of eight kinds of fat acid.Its fat acid components are similar to the vegetable oil, in which the content of unsaturated fatty acid comprised 82.4% of the total fatty acid.[Conclusion] Basing on UV induced protoplast mutagenesis, we have successfully bred a new high-yielding oil strain which can make use of the low-cost carbon source, with its oil production rate 49.4% which has increased by 21.2%.深海(微)生物资源勘探与资源潜力评价项目(No.DY125-15-R-01