Comparison of uncut Roux-en-Y anastomosis and traditional Roux-en-Y anastomosis in the totally laparoscopic distal gastrectomy for gastric cancer: An analysis of multiple centers' data

目的对比非离断式(Uncut)Roux-en-Y吻合和传统Roux-en-Y吻合应用于全腹腔镜远端胃癌根治术后消化道重建的安全性和短期疗效。方法回顾性分析国内10家单位共60例远端胃癌根治术后全腹腔镜消化道重建的胃癌病人临床资料,根据消化道重建方式不同分为Uncut Roux-en-Y组(Uncut组)34例,传统Roux-enY组(传统组)26例。结果 Uncut Roux-en-Y吻合在消化道重建时间方面用时更短[(51.5±13.3)min vs.(80.4±16.2)min,P=0.000],出血量更少[(60.0±35.6)m L vs.(132.9±65.1)m L,P=0.000],且在进食半流饮食时间方面具有优势[(4.6±1.5)d vs.(7.2±2.3)d,P=0.000]。两组病人在留置胃管时间、术后排气时间、进食流质时间和术后住院时间方面差异无统计学意义。两组病人均无围手术期死亡病例,并发症发生率差异无统计学意义(8.8%vs.7.7%,P=0.875)结论 Uncut Roux-en-Y吻合和传统Roux-en-Y吻合应用于全腹腔镜下消化道重建安全可行,Uncut Roux-en-Y吻合在重建时间和控制出血量方面具有优势。Objective To compare the security and short-term effect of the digestive tract reconstruction during the totally laparoscopic distal gastrectomy for gastric cancer between the Uncut Roux-en-Y anastomosis and the traditional Roux- en- Y anastomosis. Methods The clinical data of 60 gastric cancer patients with digestive reconstruction underwent totally laparoscopic distal gastrectomy between December 2012 and June 2015 in 10 domestic hospital were analyzed retrospectively. According to the difference of digestive reconstruction,it can be divided into the Uncut Rouxen-Y anastomosis group with 34 patients and the traditional Roux-en-Y anastomosis group with 26 patients. Results In uncut Roux-en-Y anastomosis group,the operative time in digestive reconstruction was shorter[(51.5±13.3)min vs.(80.4±16.2)min,P=0.000]; the intraoperative blood loss was less[(60.0±35.6)m L vs.(132.9±65.1)m L,P=0.000];semi-liquid diet time was earlier[(4.6±1.5)d vs.(7.2±2.3)d,P=0.000]. However,there was no difference between the two groups on nasogastric tubegastrointestinaltwo groups on nasogastric tube,gastrointestinal transit,fluid diet and the duration of postoperative hospital stay. Both groups had no death case during the perioperative period and there was no significant statistical difference in the postoperative complication rates(8.8% vs. 7.7%,P=0.875).Conclusion The Uncut Roux- en- Y anastomosis and the traditional Roux-en-Y anastomosis are both safe and feasible for the digestive reconstruction under the totally laparoscopic distal gastrectomy for gastric cancer. Besides,the Uncut Roux- en- Yanastomosis has other advantages such as less reconstruction time and less bleeding.广东省科技计划项目(No.2014A020212591

纳米颗粒作为细胞内药物载体的实验研究

利用微流控芯片制备了尺寸、形状、表面物化性质一致的纳米颗粒.此类颗粒结构以磷脂为壳,聚合物为核,中间则是水分子层.通过改变试剂的加入顺序以及调控试剂流速,他们成功地制备出含水量不同的纳米颗粒.通过理论分析以及分子模拟,揭示了刚度调节纳米颗粒进入细胞的力学机制.此项研究表明纳米颗粒的刚度对细胞的内吞起着显著影响,可以用来指导细胞内药物输运载体的设计

Synthesis of Thieno［2，3-b］thiophene-diketopyrrolopyrrole-based

设计并合成了一种交叉共轭的(cross-conjugated)缺电子型聚合物单体——二溴代噻吩[2,3-b]并噻吩-吡咯[3,4-c]并吡咯(DPPTTZ)二酮,并将其分别与噻吩(T)、硒吩(Se)和N-甲基吡咯(Py)的双锡试剂进行共聚反应,获得了一类新的供体-受体(D-A)型共轭聚合物光电材料.这类材料分子的最高占有轨道(HOMO)能级较低,因此其光电器件具有较高的开路电压(Voc),稳定性好.此外,它们在紫外-可见光区有较宽的吸收,最大吸收位于波长620 nm附近;能带隙(band gap)小,分别为1.86 e V(p DPPTTZ-T)、1.83 e V(p DPPTTZ-Se)和1.85 e V(p DPPTTZ-Py).器件初步测试结果表明,上述聚合物与PC71BM组成的本体异质结聚合物太阳能电池Voc在0.68~0.81 V之间,能量转化效率(PCE)最高达3.05%(p DPPTTZ-T).Abstract Thieno［2，3-b］thiophene-diketopyrrolopyrrole ( DPPTTZ ) ，a novel cross-conjugated electrondeficient unit，has been synthesized． This unit was brominated and then copolymerized with 2，5- bis( trimethylstannyl) thiophene，2，5-bis ( trimethylstannyl) selenophene and 2，5-bis ( trimethyl-stannyl) -Nmethylpyrrole respectively，via Stille polymerzation， to form three donor-acceptor ( D-A) type copolymers， poly{ 2，5 -bis ( 2-octyldodecyl) -3-［5-( thiophen-2，5-yl) thieno［2，3-b］thiophen-2-yl］- 6-( thieno［2，3-b］ thiophen-2，5 -yl) pyrrolo［3，4 -c］pyrrole-1，4 ( 2H，5 H) -dione} ( pDPPTTZ-T) ，poly{ 2，5 -bis( 2-octyldodecyl) - 3-［5-( selenophen-2，5 -yl) thieno［2，3-b］thiophen-2-yl］-6-( thieno［2，3-b］thiophen-2，5-yl) pyrrolo［3，4-c］ pyrrole-1，4 ( 2H，5 H) -dione} ( pDPPTTZ-Se) and poly{ 2，5-bis( 2-octyldodecyl) -3-［5-( N-methylpyrrole-2， 5-yl) thieno［2，3 -b］thiophen-2-yl］-6-( thieno［2，3 -b］thiophen-2，5 -yl) pyrrolo［3，4 -c］pyrrole-1，4 ( 2H，5 H) - dione} ( pDPPTTZ-Py) ． These polymers exhibit deep HOMO energy levels ( － 5． 26 ～ － 5． 37 eV) ， leading to high open-circuit voltage ( 0． 68 ～ 0． 81 V) in OPV devices，and good stability of the devices towards atmospheric oxygen． The UV-Vis spectra show broad features with long-wavelength absorption maximum around 620 nm and optical band gap of 1． 86 ，1． 83 and 1． 85 eV for pDPPTTZ-T，pDPPTTZ-Se and pDPPTTZ-Py respectively，which give polymers a good overlap of solar spectrum． Bulk heterojunction solar cells were fabricated by blending these polymers with ［6，6］-phenyl-C71-butiric acid methyl ester ( PC71MB) ，and the power conversion efficiency ( PCE) reaches 3． 05% for the as cast pDPPTTZ-T∶ PCBM-based device without optimization，under simulated AM 1． 5 G irradiation of 100 mW/cm2 ．国家自然科学基金(基金号21367004);广西高校人才小高地建设创新团队项目(项目号GJR201147-12)资

直接偶联法制备配基密度可控的丁基琼脂糖层析介质

以快流速琼脂糖凝胶为基质,采用直接偶联法制备丁基琼脂糖疏水层析介质.考察了影响反应的主要因素,通过四因素三水平正交实验和单因素实验,确定丁基琼脂糖疏水层析介质的优化反应条件为:反应温度45℃,反应时间45min,催化剂三氟化硼乙醚用量0.02mL/g.在此条件下严格控制琼脂糖凝胶的含水量,改变丁基缩水甘油醚用量,制备了不同丁基密度的丁基琼脂糖疏水层析介质.用不同密度的系列介质考察对牛血清白蛋白的吸附性能,初步研究了配基密度与吸附性能的关系;研究了疏水层析介质的机械稳定性和化学稳定性.结果表明,其各项性能良好,具有广阔的应用前景

直接偶联法制备配基密度可控的丁基琼脂糖层析介质

以快流速琼脂糖凝胶为基质,采用直接偶联法制备丁基琼脂糖疏水层析介质.考察了影响反应的主要因素,通过四因素三水平正交实验和单因素实验,确定丁基琼脂糖疏水层析介质的优化反应条件为:反应温度45℃,反应时间45min,催化剂三氟化硼乙醚用量0.02mL/g.在此条件下严格控制琼脂糖凝胶的含水量,改变丁基缩水甘油醚用量,制备了不同丁基密度的丁基琼脂糖疏水层析介质.用不同密度的系列介质考察对牛血清白蛋白的吸附性能,初步研究了配基密度与吸附性能的关系;研究了疏水层析介质的机械稳定性和化学稳定性.结果表明,其各项性能良好,具有广阔的应用前景

直接偶联法制备配基密度可控的丁基琼脂糖层析介质

以快流速琼脂糖凝胶为基质,采用直接偶联法制备丁基琼脂糖疏水层析介质.考察了影响反应的主要因素,通过四因素三水平正交实验和单因素实验,确定丁基琼脂糖疏水层析介质的优化反应条件为:反应温度45℃,反应时间45min,催化剂三氟化硼乙醚用量0.02mL/g.在此条件下严格控制琼脂糖凝胶的含水量,改变丁基缩水甘油醚用量,制备了不同丁基密度的丁基琼脂糖疏水层析介质.用不同密度的系列介质考察对牛血清白蛋白的吸附性能,初步研究了配基密度与吸附性能的关系;研究了疏水层析介质的机械稳定性和化学稳定性.结果表明,其各项性能良好,具有广阔的应用前景

Tunable rigidity of (polymeric core)-(lipid shell) nanoparticles for regulated cellular uptake

Core-shell nanoparticles (NPs) with lipid shells and varying water content and rigidity but with the same chemical composition, size, and surface properties are assembled using a microfluidic platform. Rigidity can dramatically alter the cellular uptake efficiency, with more-rigid NPs able to pass more easily through cell membranes. The mechanism accounting for this rigidity-dependent cellular uptake is revealed through atomistic-level simulations.</p

大连极紫外相干光源

先进光源的发展在前沿科学研究中发挥的作用越来越重要。近十年来,飞速发展的自由电子激光技术为科学家们提供了探索未知世界、发现新科学规律和实现技术变革的重要工具。建成的大连极紫外(EUV)相干光源的运行波段为50~150nm,单脉冲能量大于100μJ,且可提供10-12 s和10-13 s量级的超快激光脉冲,是我国第一台自由电子激光用户装置,并且是国际上唯一运行在极紫外波段的自由电子激光用户装置,在世界范围内为用户提供具有高峰值亮度和超短脉冲的极紫外激光。大连EUV相干光源是由国家自然科学基金委资助、由中国科学院大连化学物理研究所和上海应用物理研究所共同承担的重大科学仪器研制项目,目标是打造一个以先进极紫外光源为核心、主要用于能源基础科学研究的光子科学平台