Experiment study of the Impact of Raf /Mek/Erk Signal Pathway in the Repairing of Spinal Cord Injury

目的：既往研究发现Raf/Mek/Erk信号传导通路在脊髓损伤后出现高表达，该通路可能对脊髓损伤后神经功能恢复具有十分重要的作用。为研究Raf/Mek/Erk信号传导通路对脊髓损伤功能恢复的影响，本实验构建脊髓损伤大鼠模型,初步研究应用Raf抑制剂抑制该信号通路对脊髓损伤神经功能恢复的影响，进一步探寻脊髓损伤治疗的新靶点。 方法：选择雌性SD大鼠108只。将实验动物随机分为假手术组（仅掀开椎板，不损伤脊髓，I组，n=36）、脊髓损伤对照组（损伤脊髓，不应用raf抑制剂，II组，n=36）、脊髓损伤后PLX4720干预组（损伤脊髓，应用raf抑制剂，III组，n=36），各组又分为伤后1d，伤...Objective: Previous studies have been proved that Raf / Mek / Erk signaling pathways with high expression after spinal cord injury, this pathway may play a very important role of functional recovery after spinal cord injury. To study the role of Raf / Mek / Erk signaling pathways in the functional recovery after spinal cord injury, we build a experimental model of spinal cord injury in rats to hav...学位：医学硕士院系专业：医学院_外科学学号：2452012115319

航空叶片制造工艺及坐标检测方法分析

以当前航空发动机制造现状为研究对象,简要概述电解加工、数控加工、精密锻造及超塑性成形等叶片主流加工工艺及其制造关键性技术,并引出发动机叶片制造过程的测量手段。阐述三坐标测量过程中叶片坐标定位、测量轨迹规划及点云降噪的实现方法;简要分析了激光测头安装精度、激光投射角度、测量景深等对激光扫描法测量精度的影响。对航空叶片精密制造工艺及与之相适应的高效精密检测技术快速发展具有一定指导意义

Radioimmunoimaging of 131 I Labeled Monoclonal Antibody JHⅢFab′ in Nude Mice Bearing Human Hepatocellular Carcinoma

用蛋白酶切技术制备MCAbJHⅢfAb′片段，回收率约10%。片段活性相当于完整MCAb的1102稀释浓度。用氯胺T法标记MCAbJHⅢfAb′，对荷人肝细胞癌bEl-7402裸鼠体内的放射免疫显像进行了研究。腹腔注射131I-MCAbJHⅢfAb′后24~96H，肿瘤部位有放射性浓聚，以48~72H影像最为清晰；而注射131I-MCAbJHⅢ肿瘤部位也有积聚现象，但本底消除更慢。131I-MCAbJHⅢfAb′注射后72H，13种器官的肿瘤组织与非瘤组织的放射性比值均大于3，肿瘤的定位指数为2.56。这表明MCAbJHⅢfAb′在肝细胞癌的诊断和指导治疗方面有更好的应用前景McAb JHⅢFab′ Fragment was prepared with pepsin digestion It′s receiving percentage was about 10% Its titer was 1∶10 2 of McAb JHⅢ McAb JHⅢFab′ Fragment was radiolabeled with 131 I by Ch T method Its distribution and radioimmunoimaging in the nude mice bearing human hepatoma BEL 7402 were studied Between 24-96 h aFter intraperitoneal administration, the image of the concentrating radioacitivity in tumor was obtained by the γ camera and it was the clearest during 48-72 h; whereas the injection of 131 I McAb JHⅢ into the nude mice resulted in an equal radioactivity in tumor, but it′s background was more slowly cleared. At 72 h aFter the injection of 131 I McAb JHⅢFab′, the radioactivity ratioes of tumor/normal tissue(T/NT) For 13 kinds of organ/tissue were all over 3, and the localization index of tumor was 2 56 These results showed that McAb JHⅢFab′ may be useFul in the localization and targeting treatment of human hepatocellular carcinom

大数据多维度下的智慧交通

一、背景——城市交通现状如果想要出行便捷,汽车一定会是我们的首选工具,但是随着时间和科技的推移,如今的汽车却给人类带来了另一个大麻烦——耗费太多时间在堵车和等红绿灯上了。由于城镇化建设的不断推进和国民经济的快速发展,近年来大中型城市的机动车数量增幅巨大。城市拥堵、违规违章、事故频发、尾气排放日趋严重,这些看似无关痛痒的“城市病“,却成为文明城市进一步发展的阻碍。以北京

干扰素防治草鱼出血病的效果初探

对人工诱导的鲫鱼干扰素防治草鱼出血病的效果进行了初步探讨。结果表明 ,人工诱导的鲫鱼干扰素确能有效防治草鱼出血病 ,腹腔注射和药浴浸泡两种途径均有一定效果。在病毒感染前使用干扰素处理的效果好于在病毒感染后使用干扰素处理。多次使用干扰素处理的效果好于一次性处

抗肺癌单克隆抗体在裸鼠移植瘤中的放射免疫显像

抗肺癌单克隆抗体在裸鼠移植瘤中的放射免疫显像俞浩，瞿跃进，颜江华，盛荣宗，林月华俞丹，苏新辉，毛利娜，苏金华，杨善民我们用131I标记抗肺癌单克隆抗体（MCAb3d3）在移植人肺腺癌裸鼠中进行显像，并研究裸鼠体内生物学分布，现将结果报道如下.材料和方..

Down-regulation of TSPO expression doesn't affect the productions of TNF-α,IL-1β and IL-6 in LPS-stimulated BV-2 microglia

目的研究相对分子质量(Mr)18 000转运蛋白(TSPO)基因表达下调对脂多糖(lPS)诱导bV-2小胶质细胞分泌Tnf-α,Il-1β和Il-6的影响。方法以rnA干扰技术建立TSPO基因表达下调的细胞模型,实时荧光定量PCr(QrT-PCr)和WESTErn blOT法检测转染TSPO SIrnA bV-2细胞TSPO MrnA及蛋白水平表达的效果;用QrT-PCr法检测TSPO基因下调后小胶质细胞bV-2在lPS作用下分泌Tnf-α、Il-1β和Il-6的MrnA水平表达的情况;ElISA检测TSPO基因下调小胶质细胞bV-2在lPS作用下分泌Tnf-α、Il-1β和Il-6的蛋白水平表达的变化。结果成功建立了TSPO基因下调的细胞模型,稳定表达TSPO SIrnA细胞的TSPO MrnA和蛋白水平表达均明显下降,TSPO基因下调后bV-2细胞在lPS作用下分泌Tnf-α、Il-1β和Il-6的量无变化。结论下调TSPO的表达对lPS刺激引起的小胶质细胞Tnf-α、Il-1β和Il-6的分泌无明显影响。Objective To evaluate the effect of translocator protein( TSPO,Mr18 000) on the productions of TNF-α,IL-1βand IL-6 in lipopolysaccharide( LPS)-stimulated BV-2 cells.Methods RNA interference technique was used to decrease TSPO expression in BV-2 cells.Western blotting was performed to assess the level of TSPO protein in BV-2 cells transfected with TSPO siRNA.Then the mRNA and protein levels of TNF-α,IL-1β,and IL-6 were measured in LPS-stimulated BV-2 cells by real-time quantitative PCR( qRT-PCR) and ELISA,respectively.Results The level of TSPO protein obviously decreased in BV-2 cells transfected with TSPO siRNA.However,knockdown of TSPO had no effect on the productions of TNF-α,IL-1βand IL-6 in LPS-stimulated BV-2 cells.Conclusion Down-regulated TSPO is not directly involved in regulating TNF-α,IL-1βand IL-6 productions induced by LPS in microglia.国家自然科学基金(81071182); 福建省医学创新项目(2009-CXB-46); 厦门大学生命科学学院细胞生物和肿瘤工程教育部重点实验室开发基金(2009101); 福建医科大学非直属附属医院科研发展专项基金(2008031

铜绿微囊藻的某些生长特性及毒性的研究

<正> 藻类毒素的研究随着有毒藻类对人类的危害加剧,越来越受到各国的重视和深入研究。铜绿微囊藻(Microcystis aeruginosa)是世界许多地方报到最多的有毒藻类,对导致其毒素产生的因子及产毒机理的研究,几十年来在温度、pH值、生长年龄、光照强度、质粒、伪空胞与铜绿微囊藻毒性关系做了大量工作,但是对营养元