Satellite remote sensing-assisted comparative monitoring of dynamic characteristics of macroalgae aquaculture in Weihai City,Shandong Province,China

Monitoring the spatio-temporal dynamic changes in macroalgae aquaculture is crucial to its environmental management.However,few studies have been reported on the comparative monitoring of different macroalgae species.Based on images of the Sentinel-2 satellite and using the normalized difference vegetation index (NDVI) and the support vector machine (SVM),this study monitored the dynamic characteristics of both the Porphyra aquaculture area in the sea area of southern Wendeng District,Weihai City,Shandong Province and the kelp aquaculture area in the sea area of southern Rongcheng City,Weihai City.The results show that:① The Porphyra aquaculture in Wendeng District was first captured in the satellite images of 2016,which is the same as the first year of Porphyra aquaculture in this city;the extraction method used in this study performed well in extracting the information about both the Porphyra and the kelp aquaculture areas overall,with the overall accuracy of 84% and above;② During 2017-2021,the Porphyra aquaculture area monitored through remote sensing increased year by year and showed a trend far away from the shore;③ The Porphyra and kelp aquaculture areas monitored both showed seasonal variations (high in winter and low in summer) of cold-water macroalgae aquaculture,but the minimum and maximum values of the Porphyra aquaculture area appeared 1 ~2 months earlier than those of the kelp aquaculture area.Compared with statistical yearbooks,satellite remote sensing can provide more accurate spatio-temporal information on macroalgae aquaculture.This study can be used as a reference in terms of monitoring technology and data for the management of macroalgae aquaculture in coastal areas of northern China

不同因素对酶联免疫法测定破伤风免疫球蛋白活性的影响

目的考察不同因素对酶联免疫法（ELISA）测定破伤风免疫球蛋白生物活性的影响。方法将破伤风免疫球蛋白加入不同pH的缓冲液、不同浓度的氯化钠、硫酸铵以及聚乙二醇（PEG，相对分子质量为1000，6000和10000）溶液，采用ELISA法测定其活性。结果pH5～9的缓冲溶液对活性测定的影响不大，而pH≤4和≥10的缓冲溶液都会使测定值显著降低，当pH〈2或pH〉12时，活性下降到正常值的10％以下。随氯化钠浓度的增加测定值先下降到正常值的37．3％，后回升到41．2％，再下降；随硫酸铵浓度的增加测定值先下降到正常值的67％，后回升到99．4％，再下降。PEG对活性测定有较大影响，随PEG浓度的增加活性测定值降低。结论缓冲液pH、盐以及PEG对破伤风免疫球蛋白活性测定有影响

不同因素对酶联免疫法测定破伤风免疫球蛋白活性的影响

目的考察不同因素对酶联免疫法(ELISA)测定破伤风免疫球蛋白生物活性的影响。方法将破伤风免疫球蛋白加入不同pH的缓冲液、不同浓度的氯化钠、硫酸铵以及聚乙二醇(PEG,相对分子质量为1 000,6 000和10 000)溶液,采用ELISA法测定其活性。结果pH 5~9的缓冲溶液对活性测定的影响不大,而pH≤4和≥10的缓冲溶液都会使测定值显著降低,当pH<2或pH>12时,活性下降到正常值的10%以下。随氯化钠浓度的增加测定值先下降到正常值的37.3%,后回升到41.2%,再下降;随硫酸铵浓度的增加测定值先下降到正常值的67%,后回升到99.4%,再下降。PEG对活性测定有较大影响,随PEG浓度的增加活性测定值降低。结论缓冲液pH、盐以及PEG对破伤风免疫球蛋白活性测定有影响

中国黄土高原地帯におけるトロンの線量寄与

第46回大