Preparation,characterization,and application of monoclonal antibodies against human decoy receptor 3

目的制备抗DcR3单克隆抗体(mAb),鉴定其生物学特性,并应用于ELISA、Western blot、Flowcytometry(FCM)检测。方法以纯化的可溶性DcR3(sDcR3)免疫Balb/c小鼠,采用杂交瘤技术制备抗DcR3 mAb。用Ig亚类ELISA试剂盒鉴定抗DcR3mAb的亚类。用ELISA方法测定抗DcR3 mAb与sDcR3结合的特性,SDS-PAGE鉴定抗DcR3 mAb与SW480细胞上清中DcR3结合的特性,以鉴定mAb的特性。用间接ELISA法检测腹水mAb的效价、亲和常数并进行表位分析。Western blot检测mAb的特异性及应用,并用所获抗DcR3单克隆抗体(mAb)通过流式细胞仪检测肿瘤细胞表面DcR3的表达水平。结果获得4株可分泌DcR3 mAb的杂交瘤细胞系ZZ-393、ZZ-394、ZZ-151和ZZ-268。其中DcR3 mAb ZZ-268(下文简称为ZZ-268)的Ig亚类为IgG1(κ型);腹水效价为1×10-5;亲和常数为1.28×109水平;ZZ-268和ZZ-151可识别与其他2种抗体不同的抗原表位;Western blot证实,获得的ZZ-268... 【英文摘要】 Objective To prepare monoclonal antibodies mAb against DcR3,characterize their biological properties and apply them to ELISA,Flow cytometry,and Western blot detection.Methods Balb/c mice were immunized with soluble DcR3(sDcR3) for preparing mAb by using hybridoma technique.Ig subclass and specificity of mAbs were analyzed by ELISA and SDS-PAGE,respectively.The titres,relative affinity,and epitopes of mAbs in ascitic fluid were determined by indirect ELISA.The specificity and application of mAb were detected...厦门大学科研启动资金(Z03103);; 厦门市科技计划项目(3502Z20083008)资

东北北部冻土退化与寒区生态环境变化

我国东北地区位于中高纬度欧亚大陆东缘,大、小兴安岭多年冻土区是欧亚大陆高纬冻土区向南最突出的部位,属于高纬山地冻土.东北多年冻土区是我国,乃至全球范围内,受气候变暖和人为活动影响最显著的冻土区之一.过去40 a来该区冻土显著退化,主要表现在:1)冻土南界及不连续多年冻土各分区边界北移而导致总面积减小、空间分布破碎化;2)活动层加深,融区扩大,局地冻土岛消失;3)冻土温度升高、厚度减薄、热稳定性降低等.由于各种因素的共同影响,寒区生态环境也发生了一系列变化.这具体表现为以兴安落叶松占绝对优势的天然林带锐减,整个北方森林带北移,沼泽湿地面积减小等,寒区生态系统和环境已出现恶性循环.关注、研究、整治和管护寒区环境对区域社会、经济和生态可持续发展不可或缺

Electrocatalysis of NanoTin Dioxide in the Battery Reaction of Zinc-Nitrobenzene

以氯化锡为原料，四丙基溴化铵为表面活性剂水热法制备纳米二氧化锡（SnO2）催化剂，并以钛网为基材，制备催化电极. 应用SEM，XRD等手段对催化剂进行表征. 考察了反应物浓度、反应温度和反应时间对催化剂形貌的影响. 研究了纳米SnO2催化剂对锌还原硝基苯原电池反应的电催化性能. 结果表明，当 NaOH浓度为0. 5 mol•L-1、水热反应温度160 ℃、水热反应时间15 h时，得到的SnO2催化剂是由纳米片构成的刺球状颗粒，粒径最小，约17 nm. 与平板铂电极相比，制备的催化电极对硝基苯电还原具有更高的催化活性，硝基苯转化率为74%，最大放电功率为21.9 mW•cm-2，远大于平板铂电极. 硝基苯的主要还原产物为苯胺、对乙氧基苯胺和对氯苯胺.The tin dioxide (SnO2) nanoparticles were synthesized by using a simple hydrothermal route in the presence of tetrapropyl ammonium bromide (TPAB) as a surfactant. Accordingly, the titanium mesh based SnO2 catalyst electrode was prepared. The morphologies and structures of SnO2 nanostructures were characterized by scanning electron microscopy and X-ray diffraction spectrometry. The influences of reactant concentration, reaction temperature and time on the morphology of the products were investigated in detail. The electrocatalytic performance of SnO2 for the reduction of nitrobenzene with zinc was studied. Possible formation process and growth mechanism for such hierarchical SnO2 nanostructures have been proposed based on the experimental results. The results showed　that when the concentration of NaOH was 0.5 mol•L-1, the hydrothermal reaction temperature was 160 ℃, hydrothermal reaction time was 9 h, the as-prepared SnO2 catalyst appeared thorny spheric particles consisting of nanosheets with the particle size as small as 17 nm. Compared with Pt electrode, the catalyst electrode exhibited higher catalytic activity toward the electrochemical reduction of nitrobenzene. The conversion rate of nitrobenzene was up to 74% and the maximum discharge power density was 21.9 mW•cm-2, which are much better than those with platinum electrode. The main reduction products of nitrobenzene were aniline, p-phenetidine and p-chloroaniline.江苏省自然科学基金项目（BK20141261）、江苏省产学研前瞻性项目（BY2015057-35）、盐城市科技项目（YKA201219）资助作者联系地址：1. 江苏大学 化学化工，镇江 212013; 2. 盐城工学院，化学化工，*-盐城 224051；3. 宁波检验检疫科学技术研究院，宁波 315012；4. 江苏索普(集团)有限公司, 镇江Author's Address: 1.Department of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China; 2. Department of Chemistry and Chemical Engineering, Yancheng Institute of Technology,Yancheng 224051, Jiangsu, China; 3. Inspection and Quarantine Science and Technology Research Institute of Ningbo, Ningbo315012, Zhejiang, China; 4.Jiangsu Sopu Co.Ltd, Zhenjiang 212013,Jiangsu,China通讯作者E-mail：[email protected]

中国脑血管病临床管理指南（第2版）（节选）——第5章 脑出血临床管理 Chinese Stroke Association Guidelines for Clinical Management of Cerebrovascular Diseases (Second Edition) (Excerpt) ——Chapter Five Clinical Management of Intracerebral Hemorrhage

自发性脑出血是卒中的一种严重亚型，在中国卒中患者中占23.4%，以急性发病、病情迅速变化以及高致死和致残率为显著特点。鉴于管理脑出血所面临的紧迫和复杂挑战，本指南旨在为其临床处理提供系统性和全面性的推荐意见。本章节覆盖了从脑出血院前评估、医疗干预到二级预防和康复的各个关键环节。在具体推荐方面，本指南根据不同的证据等级，为早期诊断、影像学评估、急性期干预、内科和重症监护、外科干预以及二级预防策略提供了指导，旨在全面提升自发性脑出血诊疗的科学性和系统管理质量。 Abstract: Spontaneous intracerebral hemorrhage represents a severe subtype of stroke, accounting for approximately 23.4% of stroke cases in China. It is characterized by its rapid onset, swift disease progression, and high rates of mortality and disability. Given the urgency and complexity in managing intracerebral hemorrhage, this guideline aims to provide systematic and comprehensive recommendations for its clinical management. This chapter covers all key aspects ranging from pre-hospital evaluation and medical intervention to secondary prevention and rehabilitation. With respect to specific recommendations, this guideline offers exhaustive guidance based on varying levels of evidence for early diagnosis, imaging assessments, acute-phase interventions, medical and ICU management, surgical interventions, and secondary prevention strategies, with the ultimate aim of enhancing the scientific rigor and systematic quality of spontaneous intracerebral hemorrhage management