Preparation and selection of the monoclonal antibody used for kits to detect BNP32 which was applied to clinical research

目的:制备抗脑钠肽(bnP32)单克隆抗体(MAb),并利用双抗体夹心ElISA法建立bnP抗原检测技术,应用于临床心脏患者脑钠肽水平的检测。方法:以基因工程原核重组表达bnP抗原免疫bAlb/C小鼠,利用常规杂交瘤技术制备MAb,MAb经纯化和HrP标记后,利用双抗体夹心ElISA法筛选检测bnP32蛋白的最佳配对MAb,以其建立bnP32抗原检测技术,并与临床bnP检测的标准实验做平行比较。结果:成功筛选到16株稳定分泌抗bnP32MAb的杂交瘤细胞株,16株MAb的亚型分别为Igg1、Igg2A和IgM,并从中筛选出最佳MAb配对组合,该组合对bnP32蛋白的检测灵敏度为20ng/l。建立的双抗体夹心bnP检测ElISA法与临床bnP检测的标准实验平行比较具有很好的一致性(kAPPA值=0.828),两者没有统计学意义(P>0.05)。结论:成功地建立了bnP32抗原的双抗体夹心ElISA法检测技术,并能够很好地运用于临床心衰患者bnP指标的检测。AIM:To prepare the monoclonal antibody(mAb)used for kits to detect the BNP32 antigen by means of double-antibody sandwich ELISA assay.Comparasion differences on detection BNP between double-antibody sandwich ELISA and Roche ECL.METHODS:BALB/c mice were immunized with purified recombinant BNP32 protein and by routine hybridoma technique,Then comparasion differences on detection BNP between double-antibody sandwich ELISA and Roche ECL.RESULTS:16 hybridoma cell lines secreting potent mAb against BNP32 were obtained.The subtype of these 16 mAb were found to be IgG1,IgG2a and IgM,then their cross-blocking properties were analyzed,when they reacted with the BNP32 protein in direct ELISA in order to offer the valuable data for selecting feasible pair of mAb in the detection of the BNP32 antigen.All the mAbs were used for the detection of BNP32 by double-antibody sandwich ELISA.In addition,the mAbs were purified and HRP-labelled in advance.Finally,we had successfully screened a pair of mAbs,which exhibited a sensitivity of 20 ng/L for the detection of BNP32 antigen.The two detection methods have very good consistency(kappa=0.828>0.75)between double-antibody sandwich ELISA and Roche ECL.There was no statistics significance on differences between these two methods(P>0.05).CONCLUSION:It is evident that this pair of mAb shows excellent detection of BNP32.The double-antibody sandwich ELISA can be good apply to detect BNP level in clinical congestive heart failure patients

DNA-PKcs基因组不稳定性和辐射超敏感性

本研究旨在揭示DNA-PKcs(DNA损伤修复的关键酶之一)与基因组不稳定性、辐射超敏感性的关系。以人神经胶质瘤细胞系M059K(DNA-PKcs野生型)和M059J(DNA-PKcs缺陷型)为实验模型,用常规克隆形成法测定细胞存活率、cytochalasin-B(细胞松弛素B)阻断-微核法跟踪克隆形成过程中基因组不稳定性变化。实验结果表明,存在辐射超敏感性的DNA-PKcs野生型细胞M059K在0.2Gy辐照后随着培养时间的延长基因组不稳定性与0.6Gy辐照水平一致;不存在辐射超敏感性的DNA-PKcs缺失型细胞M059J经0.6Gy辐照后,基因组不稳定性水平明显高于0.2Gy辐照。这些结果提示,DNA-PKcs可能是导致辐射超敏感性的关键因素之一

基于立体定向脑电图的尴尬先兆脑网络分析

&lt;正&gt;病例报道:患者女性,28岁,11年前出现癫痫发作,表现为尴尬（秘密被其他人偷窥）先兆,随后出现脸红,意识受损,遮盖脸部,避免与他人接触。发作持续10-20秒,偶有演变成双侧强直性阵挛性发作,药物治疗无法控制发作,病史和体格检查正常。非侵入性评估结果:发作期脑电图提示左后颞区的短暂快活动,头颅核磁阴性,PET-CT示左额叶代谢减低,脑磁图示左颞部少量的偶极子。&nbsp;</p

Preliminary Study of ~(41)Ca-AMS Measurement in Rock Samples

41CA在核天体物理及地质年代学方面可能具有较高的应用价值。为测量天然岩石样品中41 CA的本底水平,探讨了41CA-AMS测量中岩石样品的CAf2制备,提出了二次氟化的制备方法。在此基础上,通过对导电介质与靶锥的改进,设计了一种提高CAf-3束流引出强度的方案。该方案可有效提高CAf-3束流引出强度。实验结果表明,天然岩石样品中41CA/40CA(41CA、40CA原子个数比)的本底水平低于8x10-14。Perhaps the value of 41Ca applications in nuclear astrophysics and geochronology is high.The CaF2preparation of rock samples was investigated to measure the background level of 41Ca in the natural rock samples by AMS.A preparation method of secondary fluorination was proposed by some physical and chemical processes.On this basis,aprogram to improve CaF-3beam intensity was designed by improving conductive medium and target cone.This sample preparation program can effectively improve the beam intensity of CaF-3.The experimental results show that with these settings the41Ca/40Ca background level of rock samples is less than 8×10-14.国家自然科学基金资助项目(11265005); 广西自然科学基金资助项目(2012GXNSFBA053001

Accelerator Mass Spectrometry Measurement of --(53)Mn and Its Applications in Earth Sciences

宇宙成因核素53Mn是一种非常理想的地貌演变研究工具。近年来,随着加速器质谱(AMS)技术的不断发展,人们已能够进行53Mn的高灵敏测量。介绍了53Mn在地球科学中的应用前景、国际研究现状及中国原子能科学研究院目前正在开展的53Mn高灵敏AMS测量方法的研究。The determination of cosmogenic 53Mn concentrations found in terrestrial formations has many interesting applications for Earth Sciences.With Accelerator Mass Spectrometry(AMS) it should be possible to detect minute amounts of 53Mn.The situation of international research of 53Mn AMS measurement and applications are presented.The AMS measurement method of 53Mn at China Institute of Atomic Energy and its applications prospect are to be explored.国家自然科学基金资助项目(11075221);国家青年基金资助项目(10705054)---