Grp78/Bip介导戊型肝炎病毒衣壳蛋白对宿主细胞的吸附

目的探讨Grp78/Bip在戊型肝炎病毒(HEV)衣壳蛋白进入宿主细胞过程中的作用。方法采用pull-down和免疫共沉淀技术进一步验证p239与Grp78/Bip的相互作用;采用激光共聚焦显微镜技术检测p239与细胞膜表面Grp78/Bip共定位情况;采用原核表达纯化的Grp78/Bip蛋白封闭p239的Grp78/Bip结合位点,检测其阻断p239吸附细胞的效果。结果p239与Grp78/Bip可以直接结合,而且这种结合是可逆的生理性结合;p239与Grp78/Bip在细胞膜上存在部分共定位;Grp78/Bip能部分阻断p239对肝细胞的吸附。结论Grp78/Bip参与并介导HEV衣壳蛋白对宿主细胞的吸附

法学家眼中的和谐社会

从现有权威性文献看,“和谐社会”是执政党要追求的一种社会状态,更是一种涉及面极其广泛的治国方略。在这个过程中,法律、法学和法学家的作用举足重轻。面对如此重大议题,法学家应当表达观点、提出诉求、发挥专业功能。基于这种考虑,本刊特邀部分中青年法学家进行了笔谈,希望他们的文章能引起讨论,促进法律界、法学界形成一些基本共识

重组戊型肝炎病毒衣壳蛋白p239特异性吸附、侵入HepG2细胞并阻断野病毒对肝细胞的感染

By using Western blot and immunofluorescence assays,the recombinant HEV capsid protein p239 was found specifically attached to the HepG2 cell surface and entered to the cytoplasm with the increase of incubation temperature.Pre-mixture of wild-type HEV with p239 blocked the infectivity of the virus on primary cultured human hepatocytes and HepG2 cells,indicating that p239 and HEV competed the same targeting site on these cells.These data provide evidence that p239 has a similar cell surface structure with wild-type HEV

A COMPUTATIONAL MODEL FOR SPINNING EFFECTS ON POST-COLLISION VELOCITIES OF BOUNCING DROPLETS

The present study proposed a computational model for the spinning effects on the post-collision characteristics (particularly velocities) of the bouncing droplets. The modeling work is based on the computational discoveries by using the validated volume-of-fluid method to simulate droplet collisions with various collisional parameters. The physical foundation of the model is based on the fact that the interchange between the orbital angular momentum and the spin angular momentum plays an important role in determining the post-collision velocities. First, the conversion of the orbital angular momentum into its spinning counterpart varies nonmonotonically with the impact parameter of two nonspinning droplets. Second, the conversion of the spin angular momentum into its orbital counterpart is significant for the collision between two spinning droplets. Furthermore, this spin-to-orbital angular momentum conversion varies monotonically with the azimuthal angle and is approximately independent of the translational Weber number, the spinning Weber number, and the Ohnesorge number. These computational discoveries have been analyzed for their physical meaning and taken into account in the proposed model

Analysis of spray characteristics of a jet film injection element based on Voronoi tessellation

The present paper presents a computational study based on a validated Volume of Fluid method on the effects of film thickness and film width on spray characteristics for the simplified jet film injection element with varying mass flow rate conditions. First, the spray characteristics of a typical jet film collision were analyzed in terms of the spatial distribution of Sauter Mean Diameter and Voronoi tessellation. Both the Voronoi diagram and the Sauter Mean Diameter contour are useful to evaluate the spray characteristics because they show two different aspects of the sprays. Second, the jet film spray characteristics were analyzed by decreasing either film thickness or film width to change the mass flow rate. The results show that decreasing film width results in approximately insensitive spray angle and improved uniformity of droplet distribution for different throttleable levels. The present computational results verified our design concept that adopting traditional jet film injection at a large mass flow rate and modified jet jet injection at a small mass flow rate to maintain good spray performance during the entire throttleable levels

Effect of Long-term P Fertilization on Wheat Yield of Loess Plateau

以黄土高原长期定位试验为基础,研究施一定量氮肥(90 kg/hm2)的前提下,长期施用磷肥对黄土高原旱作冬小麦的肥料效应。结果表明,长期施用磷肥的农田施磷仍能显著提高小麦的产量,增产量达1393.75~2121.00kg/hm2,增产率为48.41%~73.67%,本试验中施磷39.6 kg/hm2时小麦产量达最大值5000 kg/hm2,这与小麦成穗数最大时的施肥量结果一致;产量与施磷量关系用回归方程Y=-0.8667X2+82.641X+3008.4(R2=0.92)拟合效果良好;施磷主要是通过影响小麦成穗数来影响小麦产量,而对穗粒数和千粒重的促进作用不明显;施磷还可促进小麦对氮磷钾养分的吸收利用,提高肥料的肥效和利用率;施磷过少不能满足作物需求,小麦产量较低,施磷过多小麦产量不会随施肥量的增加继续提高,反而有一定幅度的下降