RESEARCH ON HIGH RESOLUTION TIME OF FLIGHT SPECTROMETRY WITH ELECTROSPRAY IONIZATION SOURCE

它分析方法相比，质谱分析法具有灵敏度高、分析速度快、定性分析精确和信息量丰富等特点。它经过近百年的不断发展，从最初的同位素分析，到现在已经逐渐成为化学、地质学、生物化学、药物学、医学、石油化工、能源、环保和食品加工等许多方面不可缺少的分析检测手段，是分析科学领域中最重要的工具之一。随着近代物理学、电子学、计算机、真空技术和机械加工技术的进一步发展，质谱仪的性能还在不断提高。 质谱仪主要有磁偏转式质谱、四级杆/离子阱式质谱（Q/IT-MS）、飞行时间质谱（TOF-MS）和富里叶变换离子回旋共振质谱（FTICR-MS）四种类型。而飞行时间质谱仪（TOF-MS），具有微秒级的快速检测速度、高离子传...Comparing with other analysis techniques, Mass spectrometry(MS) has many virtues, such as high sensitivity, high analysis speed, high accuracy of mass measurement and a great deal of information. After nearly a century development, its applications vary from isotope analysis preliminarily to Chemistry, Geology, Bio-chemistry, Pharmacology, Medicine, Petroleum chemistry, Energy, Environmental prote...学位：理学博士院系专业：化学化工学院化学系_分析化学学号：B19982500

The Possibility of Administrative Litigation Legislative Reference Civil Litigation Mediation System and Its Limit

《中华人民共和国行政诉讼法》(以下简称《行政诉讼法》)第50条规定：“人民法院审理行政案件不适用调解。”随着行政诉讼制度的发展，不断呈现出一系列新的阶段性特征，如许多法院积极运用协调或和解的方式解决行政争议，取得了较好地法律效果与社会效果。但这种实定法与法律实践的脱节处境非常尴尬。为此，笔者主要借助于民事诉讼调解的法理根据之解读，旨在行政诉讼立法上借鉴适用调解方式，进而析明行政诉讼法领域引进调解制度的可能性及限度，以期于行政诉讼的发展有所裨益。本文除前言和结语外，共分三个部分。第一部分，以民事诉讼制度为样本分析论证调解作为解决纠纷机制的正当性以及与审判制度相比较所具有的独特优势。第二部分，以行..."PRC Administrative Procedure Law" (hereinafter referred to as "the Administrative Procedure Law") Article 50: "People's Court administrative case does not apply mediation." With the administrative litigation system development, and constantly showing a series of new short-term characteristics, as many active use of the Court of coordination or reconciliation solution to the dispute, and achieved ...学位：法律硕士院系专业：法学院法律系_法律硕士(JM)学号：X20040807

电源线测试仪的研制

本文介绍的是一台自行研制成功的电源线测试仪，它能完成电源线电气性的质量检验工作。本测试仪已在工厂试用近1年，效果反应良好

Chronic hypoxia enhances aggressiveness of MCF-7 breast cancer cells through EMT

目的：探讨慢性缺氧应激对人乳腺癌MCF-7细胞恶性生物学行为的影响及可能机制。方法：将人乳腺癌MCF-7细胞分为缺氧组（1%O2、5%CO2和94%N2）和正常对照组（常氧）进行培养。利用MTT法、CCK-8实验、细胞直接计数法及细胞侵袭和迁移实验对MCF-7细胞活力、增殖及侵袭和迁移能力进行检测;用软琼脂集落形成实验及Matrigel 3D培养技术检测MCF-7细胞非锚定生长能力及极性改变情况;利用MCF-7细胞构建裸鼠皮下种植瘤模型,检测慢性缺氧应激对体内肿瘤生长及肺转移的影响;利用倒置显微镜观察MCF-7细胞形态改变;Western blot检测低氧诱导因子1（hypoxia-inducible factor-1,HIF-1）和磷酸化的糖原合成酶激酶3β（glycogen synthase kinase-3β,GSK-3β）在缺氧环境下表达水平的改变,以及E-钙黏连蛋白（E-cadherin）、N-钙黏连蛋白（Ncadherin）、波形蛋白（vimentin）、基质金属蛋白酶3（matrix metalloproteinase-3,MMP-3）、MMP-9等上皮-间充质转化（epithelial-mesenchymal transition,EMT）相关蛋白的表达水平。结果：与正常对照组相比较,慢性缺氧组MCF-7细胞活力、增殖能力及侵袭迁移能力增强,细胞非锚定生长能力提高且在3D培养系统更容易发生极性改变,呈现侵袭样生长,体内生长及转移能力增强;除了HIF-1被缺氧诱导表达升高外,GSK-3β呈现活化趋势,且上皮样标志物E-cadherin蛋白表达水平明显下降,而间充质样标志物N-cadherin、vimentin、MMP-3和MMP-9蛋白表达水平明显升高。结论：慢性缺氧应激促进了乳腺癌细胞恶性生物学行为,且其机制可能与EMT有关。AIM：To investigate the effects of chronic hypoxia on the aggressiveness of MCF-7, a human breast cancer cell line , and the underlying mechanisms .METHODS： MCF-7 cells were cultured under hypoxia （ 1% O2 , 5%CO2 and 94%N2 ） or control （95%O2 and 5%CO2 ） condition.The viability, proliferation, and invasion and migration abilities of the MCF-7 cells were determined by MTT assay , CCK-8 assay, cell counting, and cell invasion and migration assays.Anchorage-independent growth and the alteration of cellular polarization of the MCF-7 cells were tested by soft agar colony formation assay and Matrigel-3D culture assay, respectively.The effects of chronic hypoxia on the growth and metas-tasis of MCF-7 cells in vivo were investigated by xenograft in nude mice .The morphological changes of the MCF-7 cells were observed under an inverted microscope .Hypoxia-induced alterations in the levels of hypoxia inducible factor-1 （ HIF-1 ） and phosphorylated glycogen synthase kinase-3β（ p-GSK-3β） as well as epithelial-mesenchymal transition （ EMT） mol-ecules, such as E-cadherin, N-cadherin, vimentin, matrix metalloproteinase （ MMP）-3 and MMP-9, were determined by Western blot .RESULTS：Chronic hypoxia significantly increased the viability , proliferation , and invasion and migration abilities of MCF-7 cells in vitro, enhanced the anchorage-independent growth , facilitated cellular polarization alteration in Matrigel-3D culture, and promoted cancer metastasis in vivo.Hypoxia up-regulated HIF-1, activated GSK-3β, down-regu-lated E-cadherin and increased the protein levels of N-cadherin, vimentin, MMP-3 and MMP-9.CONCLUSION：Chronic hypoxia enhances the aggressiveness of breast cancer cells probably through EMT .中国博士后科学基金第59批面上资助项目（No.2016M592037）;安徽省自然科学基金资助项目（No.1608085MH187

一种三维纳米定位台控制系统设计

这篇文章描述了一种三维纳米定位台控制系统。本系统采用双处理器架构,将ARM在逻辑控制、人机交互的优势同FPGA硬件可编程、并行处理的优势互补,再搭载自行设计的高精度ADC电路及DAC电路,完成了对三维纳米定位台的高精度运动控制

Optical System for Asymmetrical Crossed Czerny-Turner Raman Spectrometer with High Luminous Flux

为克服分立式便携拉曼光谱仪光通量低的缺点,设计了一种集拉曼探头光路与分光系统于一体的光学系统。探头光路采用大数值孔径的非球面透镜实现样品的有效激发和信号的高效收集,通过胶合透镜组缩小会聚光路尺寸、消除轴向色差。分光系统基于交叉非对称Czerny-Turner结构,为获得期望的光谱分辨率和光谱范围,建立了分光系统光谱分辨率及光谱范围与交叉非对称Czerny-Turner结构参数的关系。由测得的汞灯谱图可知,分光系统的光谱分辨率优于6 cm~(-1)(0.37 nm),光谱范围为790~950 nm(200~2000 cm~(-1))。将设计的光学系统对CCL4进行测试,实验结果表明在相同积分时间内由这种整体式的光学系统检测到的CCL4光谱谱峰强度是用商业探头通过光纤连接分光系统检测到的近3倍,验证了光学系统设计的合理性。To overcome the low luminous flux in separated portable Raman spectrometer, an optical system embodies the probe of Raman spectrometer and monochromator is designed. An aspherical lens with high numerical aperture is used to obtain the effective excitation of sample and the efficient collection of signal in the optical system of the probe. A cemented lens group is used to minish the size of converging path and eliminate chromatic aberration.The monochromator is based on asymmetrical crossed Czerny-Turner structure. For the purpose of achieving desired spectrum resolution and spectrum range, the relationship between the spectrum resolution and spectrum range of the monochromator and the structural parameters of the asymmetrical crossed Czerny-Turner system is established. According to the tested spectrogram of mercury lamp, the spectrum resolution of the monochromator is better than 6 cm~(-1)(0.37 nm), and spectrum range is 790~950 nm(200~2000 cm~(-1)). A sample of CCL4 is introduced to examine the performance of the optical system, the experimented results demonstrate that the peak intensity of spectrogram of CCL4 measured by this incorporative optical system is almost three times as high as that using a commercial probe couples to the monochromator, which verifies the rationality of the design of the optical system.国家自然科学基金(21503171);; 中央高校基本科研业务费项目(20720150091、20720150094);; 福建省高端装备制造协同创新中心资

卷积神经网络模型在儿科疾病预测中的应用

目的:针对儿童看病需求量大导致的儿科诊疗服务效率和准确率偏低等问题,利用自然语言处理和深度学习技术,从儿科历史病历数据中自动\"学习\"专家医生诊断模式,形成智能辅助诊断模型,从而对新的儿科病历数据输出疾病诊断决策。结果:基于深度卷积神经网络的七分类疾病智能诊断模型的正确率为84.26%,F1-score为84.33%,基本达到可投入实际应用的级别。结论:智能诊断决策作为预诊信息提供给医生进行确诊参考,对提升医生诊断速度效果明显。国家自然科学基金面上项目(编号:71571056);;福建省自然科学基金面上项目(编号:2012J01274)~

Effects of mixed-culture densities of Spartina alterniflora on eco-physiological characteristics of Kandelia candel seedling

按照不同密度将互花米草与秋茄进行混种,对比研究胁迫条件下秋茄幼苗生长、光合特性及其渗透调节物质变化规律。结果表明,低密度互花米草促进秋茄的茎长以及各部分生物量,高密度则起抑制作用。随着互花米草密度的增大,秋茄幼苗叶片光合速率、气孔导度、蒸腾速率、水分利用率和蛋白含量均下降;相反,胞间CO2浓度、可溶性糖、淀粉、脯氨酸含量却上升,这些变化有利于对抗互花米草带来的不利影响。 【英文摘要】 Kandelia candel seedlings were mix-cultured with Spartina alterniflora of different densities for 120 days,and their growth,photosynthetic characteristics as well as osmotic adjustment law were studied.The results showed that the stem height and biomass of K.candel seedlings were enhanced with low density S.alterniflora and inhabited with high density ones.With the density of S.alterniflora going up,Pn、Gs、Tr、WUE and protein content in K.candel seedlings decreased respectively.On the contrary,the content of ...国家自然科学基金资助项目(30670317,30271065);; 福建省自然科学基金资助项目(D0210001

Development of TOF-MS Used as the Detector of HPLC

本文研制的质谱仪是具有电喷雾离子源和射频四极杆接口的高分辨飞行时间质谱仪(ESI-QQQ-TOF-MS)。该仪器的特点如下:采用可三维调节、带有加热雾化气的电喷雾源液质联用接口;采用由三组四极杆组成、可有效调制离子束的离子光学系统;采用正负双脉冲推斥和垂直引入方式;采用经优化设计的二级有网反射器。该仪器分辨本领优于11 000(Full Width at Half Maximum,FWHM),质量测定精度优于10×10-6,最低检测限低于3 fmol/μL。可作为高效液相色谱优良的质谱检测器。Time-of-flight mass spectrometry(TOF-MS) is widely used in protein chemistry and proteomics research due to its high sensitivity,high resolution and high accuracy.Recently two Q-TOF-MS analytical systems are developed.Both systems are equipped with electrospray ionization source which could be directly hyphenated with HPLC as an outstanding detector.The ESI-triple quadrupole-orthogonal acceleration-reflecting-TOF mass spectrometer has some promising features,such as a ESI source with heatable nebulizer settled on a smart triaxiality moving stage,an excellent ion-optics system composed with triple-quadruple,double-pulse acceleration,and a double stage reflector with homemade grids.The Gramicidin-S sample concentration below to 3 fmol/μL can be identified by these instruments under the optimized conditions.The mass range of these instruments is from 50 u to 6 000 u with a general ESI interface to the HPLC.The optimal mass resolution exceeds 11 000(Full Width at Half Maximum,FWHM) and the signal/noise ratio of reserpine(10 pg/μL) is greater than 100.科技部“十五”科学仪器攻关重大项目(2001BA210A01);; 973基金(01CB510202);; 863重大专项基金(02BAC11A11);; 上海市创新团队基金(03DZ14024);; 上海市重大基础研究(04DZ14005);; 国家自然科学基金(20405003);; 863人肝专项基金(2004BA711A18);; 973人肝新技术基金(2004CB520804)支

基于表面增强拉曼光谱的合成色素专利蓝V的快速检测

食品安全问题一直是社会和广大群众关注的焦点问题,食品安全现状较为严峻,因此实现食品中有害物质的快速检测具有重要的实际意义。合成色素是一种常见的食品添加剂,然而合成色素的超标添加和非法添加依旧是食品安全中的重要问题之一,极大地危害人民群众的身体健康和食品工业的健康发展。常见的合成色素检测方法,均存在耗时长、费用高等缺点,不适应于合成色素的实时监测和快速筛查。为克服传统方法的缺点,提出利用表面增强拉曼光谱检测技术对合成色素进行检测,该方法具有检测速度快、检测灵敏度高等优点,能够达到现场实时检测的目的。此外,由于拉曼检测方法往往依赖于复杂的样品前处理操作,而常见的固相萃取技术一般依赖于人工操作,过程复杂且耗时较长,严重影响食品快速检测效率。因此,开发了一种全自动固相萃取装置,通过设计嵌入式硬件电路系统及其软件,精确控制蠕动泵流速和多路阀门开关实现了活化、上样、淋洗、洗脱四个步骤的全自动操作和参数控制,从而达到食品样品的全自动快速固相萃取。在实验部分,配制不同专利蓝V浓度的果汁饮料,然后利用该装置对果汁中的专利蓝V进行前处理,对萃取柱填料和萃取中各个步骤的时间和试剂进行了合理的选择,利用表面增强拉曼光谱检测技术成功地检测了合成色素中的专利蓝V。实验结果表明,所研制的自动固相萃取装置对比传统手工萃取,每个样品节省了近一半的萃取时间（10 min降为5 min）且能够同时处理5个样品,萃取时间稳定不易受人为因素影响,从而极大地提高了萃取效率和稳定性。此外,通过自动萃取获得的样品,对比手工萃取操作,因其受外界干扰相对较小,能够得到更强的拉曼光谱信号（约增强50%）,获得了满意的萃取效果。对不同浓度的专利蓝V样品的结果显示,该方法能够实现检出质量浓度在0.5 mg·L-1水平,可有效满足现场监测需求。具有快速、方便、灵敏度高等特点。国家自然科学基金项目(21874113)资