Synthesis and identification of artificial complete antigen 25-hydroxyvitamin D_3

目的:合成25-羟基维生素d3人工完全抗原,并制备抗25-羟基维生素d3的特异性抗体。方法:将25-羟基维生素d3进行化学修饰加入羧基活性基团,合成具有半抗原结构特征的25-羟基维生素d3-半琥珀酸酯。采用碳二亚胺法,将25-羟基维生素d3-半琥珀酸酯,分别与牛血清白蛋白(bSA)和卵清蛋白(OVA)偶联,合成人工完全抗原25-羟基维生素d3-半琥珀酸酯-bSA和25-羟基维生素d3-半琥珀酸酯-OVA。通过紫外吸收光谱,SdS-PAgE和MAldI-TOf进行偶联鉴定。用25-羟基维生素d3-半琥珀酸酯-bSA免疫小鼠,获得抗25-羟基维生素d3抗体免疫血清。结果:25-羟基维生素d3-半琥珀酸酯与bSA的偶联比为(12±0.16)∶1,免疫小鼠后获得高效价(效价为6.25x10-4)的抗体,且标准品浓度在37.5~600 ng/Ml范围具有显著的竞争性线性关系,检测的灵敏度为37.5 ng/Ml。结论:成功合成了25-羟基维生素d3人工完全抗原,制备出25-羟基维生素d3的抗体且其线性关系显著,灵敏度较高,为进一步研制检测25-羟基维生素d3的试剂盒奠定了基础。AIM: To synthesize the 25-hydroxyvitamin D3 artificial complete antigen and to prepare the specific antibody against 25-hydroxyvitamin D3.METHODS: The active group carboxyl was introduced into 25-hydroxyvitamin D3 and formed 25-hydroxyvitamin D3-hemisuccinate which possessed the structure of the hapten by chemical modification.The EDC method was applied to conjugate 25-hydroxyvitamin D3-hemisuccinate to bovine serum albumin as an artificial immunogen.The coating antigen 25-hydroxyvitamin D3-hemisuccinate-OVA was obtained in the same way.Ultraviolet,SDS-PAGE and MALDI-Tof were used to identify 25-hydroxyvitamin D3-hemisuccinate-BSA.RESULTS: BALB/c mice were immunized with 25-hydroxyvitamin D3-hemisuccinate-BSA to generate the polyclonal antibody of the 25-hydroxyvitamin D3 worth high titer and the immunogen,25-hydroxyvitamin D3-hemisuccinate-BSA,was successfully prepared with coupling ratio(12±0.16)∶1(N=3) coupling.CONCLUSION: The high titer and good sensitivity of anti-25-hydroxyvitamin D3 antibody are produced in sera immunized BALB/c mice,which made it possible to develop a clinical diagnostics for illness

Si( 100) 表面Se 薄膜生长及其在Ti /Si 欧姆接触中的应用

National Basic Research Program of China [2007CB613404]; National Natural Science Foundation of China [61036003, 60837001]; Natural Science Foundation of Fujian Province of China [2008J0221]We have investigated the growth of thin selenium layer on Si (100) substrate by molecular beam epitaxy (MBE). By controlling the temperatures of the silicon substrate and the selenium source during growth, an ultrathin film of Se is successfully grown on the Si(100) substrate. As the Si(100) surface is passivated by the ultrathin film of Se, the electrical property of the Ti/n-Si(100) contact is shown to be ideally ohmic, with low resistance and relatively high thermal stability

超声波均质和GOD脱糖预处理鸡蛋清

考察了超声波均质及葡萄糖氧化酶(GOD)脱糖对鸡蛋清(CEW)粘度、蛋清中溶菌酶浓度和活力的影响.结果表明,超声波均质和GOD脱糖均能有效降低蛋清的粘度,从而得到低粘度的均匀料液.但随着超声波功率的增加和超声处理时间的延长,溶菌酶的浓度及活力呈下降趋势.而经GOD脱糖处理后的料液溶菌酶活力随GOD加入量的增大而降低,浓度则先升高后下降.将两种处理方法结合使用,料液粘度可以降至4.0mPa-s,此时溶菌酶损失19.48%,酶活降低19.13%

超声波均质和GOD脱糖预处理鸡蛋清

考察了超声波均质及葡萄糖氧化酶（GOD）脱糖对鸡蛋清（CEW）粘度、蛋清中溶菌酶浓度和活力的影响．结果表明，超声波均质和GOD脱糖均能有效降低蛋清的粘度，从而得到低粘度的均匀料液．但随着超声波功率的增加和超声处理时间的延长，溶菌酶的浓度及活力呈下降趋势．而经GOD脱糖处理后的料液溶菌酶活力随GOD加入主的增大而降低，浓度则先升高后下降．将两种处理方法结合使用，料液粘度可以降至4．0mPa．s，此时溶菌酶损失19．48％，酶活降低19．13％

润滑油与变速箱同步器相适性评定技术研究进展

润滑油对变速箱同步器的性能有重要影响,开展其相适性评定工作可为变速箱同步器的合理用油提供技术支持。综述了目前国内外有关相适性评定技术在试验台架、试验部件、试验结果判定标准等方面的研究现状,讨论了现有评定技术中所用试验部件和模拟工况与我国变速箱同步器的结构类型和实际工况存在的相似点与差别,介绍了国内手动变速箱同步器油品评定技术的研究进展,展望了我国在润滑油与变速箱同步器相适性评定技术方面需要进一步完善的地方

超声波均质和GOD脱糖预处理鸡蛋清

考察了超声波均质及葡萄糖氧化酶（GOD）脱糖对鸡蛋清（CEW）粘度、蛋清中溶菌酶浓度和活力的影响．结果表明，超声波均质和GOD脱糖均能有效降低蛋清的粘度，从而得到低粘度的均匀料液．但随着超声波功率的增加和超声处理时间的延长，溶菌酶的浓度及活力呈下降趋势．而经GOD脱糖处理后的料液溶菌酶活力随GOD加入主的增大而降低，浓度则先升高后下降．将两种处理方法结合使用，料液粘度可以降至4．0mPa．s，此时溶菌酶损失19．48％，酶活降低19．13％