Ecotoxicity of degradation product from dye Reactive Black 5 by electrochemical degradation products using MFC as a source of electricity

Vodeni rastvor boje (100 mL) RB5 od 25 mg/L i 1 mM H2O2 elektrohemijski je tretiran, koristeći Pt i Fe elektrodu. MFC ćelija se koristila kao izvor struje za degradaciju. Ekotoksikološka analiza pomoću bakterije Vibrio fisheri je pokazala da najveću toksičnost ima polazna boja RB5 (EC20 8,17%), dok nastali degradacioni proizvod nakon 6 h tretiranja strujom dobijenom preko MFC sistema ima najmanju toksičnost na spomenutu bakteriju (EC20 33,05%).Aqueous dye solution (100 mL) RB5 of 25 mg / L and 1 mM H2O2 was electrochemically treated, using Pt and Fe electrode. The MFC cell was used as a current source for degradation. Ecotoxicological analysis using Vibrio fisheri showed that the highest toxicity is the RB5 dye (EC20 8.17%), while the resulting degradation product after 6 h of treatment with electricity obtained through the MFC system has the lowest toxicity to this bacterium (EC20 33.05%)Poster: [https://cer.ihtm.bg.ac.rs/handle/123456789/5270

Biochemical and molecular characterization of Klebsiella sp. isolated from environment polluted with perfluoroalkyl substances

Poster presented at: 13th Symposium “Nоvel Technologies and economic development”, October 18-19, 2019, Leskovac, SerbiaRazvoj hemijske industrije u prošlom veku doprineo je povećanoj proizvodnji hrane, efikasnijoj kontroli bolesti i poboljšanju životnog standarda. Ipak, usled industrijskog razvoja došlo je do nagomilavanja velikih količina toksičnih supstanci u životnoj sredini. Dugotrajni organski zagađivači su hemikalije koje se zadržavaju, akumuliraju u čitavom lancu ishrane i imaju štetan uticaj na ljudsko zdravlje i životnu sredinu. Perfluorovana jedinjenja, kao što su perfluoroktan sulfonska kiselina, njene soli i perfluoroktan sulfonil fluorid, nalaze se na listi dugotrajnih organskih zagađivača. Nekoliko skorašnjih publikacija je pokazalo da mikroorganizmi izolovani iz životne sredine zagađene perfluorovanim jedinjenjima mogu smanjiti nivo istih. U ovom radu je bakterijski soj izolovan iz takve životne sredine fiziološki, biohemijski i molekularno okarakterisan. U preliminarnim laboratorijskim testovima soj je pokazao sposobnost smanjenja količine perfluorovanih jedinjenja. Mikroorganizam je Gram-negativan, nepokretan, oksidaza negativan i pozitivan na katalazu, proizvodi različite hidrolazne enzime. Mikroorganizmu je određen i masno-kiselinski profil. Molekularna karakterizacija je potvrdila da izolovani soj pripada rodu Klebsiella. Mikroorganizam je uspešno okarakterisan različitim metodama i ubuduće će biti korišćen u detaljnoj laboratorijskoj studiji analize mehanizama smanjenja koncentracije perfluorovanih jedinjenja.Conference paper: [https://cer.ihtm.bg.ac.rs/handle/123456789/4994

Structural characterization of EPS produced by Brachybacterium paraconglomeratum sp. CH-KOV3

The aim of this work was structural characterization of EPS produced by Brachybacterium paraconglomeratum sp. CH-KOV3. For the structural instrumental analysis of EPS, the following methods were applied: GC-MS (gas chromatography mass spectrometry) and correlated two-dimensional NMR (nuclear magnetic resonance) techniques - DEPT 135 (distortionless enhancement by polarization transfer), COSY (correlation spectroscopy), and HSQC (heteronuclear single quantum coherence). Methylation was performed by the method which described earlier. The permethylated EPS was subjected to reductive cleavage as described by Rolf and Gray. Cleaved monomer units were acetylated. Obtained acetylated, methylated products were analyzed by GC-MS. These analyses were performed on a GCxGC-MS (Shimadzu, Kyoto, Japan). NMR spectra of the isolated EPS were measured on a Bruker AVANCE III 500 spectrometer

Bacillus sp. Isolated from Japanese food Natto

Poster presented at Serbian Biochemical Society Ninth Conference with international participation.Abstract: [https://cer.ihtm.bg.ac.rs/handle/123456789/5015

Power generation using parallel connected microbial fuel cells systems

The use of microbial fuel cells (MFCs) for the purpose of obtaining energy from nature has grown in the second decade of the 21st century. Due to the lack of fossil fuels and less and less available renewable energy sources, MFCs have great potential for obtaining small amounts of electricity. Increasing attention is focused on the use of such types of energy. The aim of this paper is to analyse the current generation of two parallel-connected MFC cells and the power generation obtained in this way

Crude extracts of cinnamon and oregano spices as components of pullulan based edible films

Pullulan based films were prepared with crude extracts of cinnamon and oregano spices. Obtained films were characterized by FTIR-ATR spectroscopy and their antioxidant and antimicrobial properties were evaluated. These biopolymer based films have potential use in food applications as food ingredients and active packaging material.6th Workshop Specific Methods for Food Safety and Quality, 27.09.2018. Belgrade, Serbi

Bacillus sp. Isolated from Japanese food Natto

Nattō is a traditional Japanese dish made from fermented soybeans and is usually combined with soy sauce. It is very rich in vitamins, amino acids, proteins, sugars, fats, minerals and dietary fibres, and polypeptides consisting of 275 amino acid residues with anticoagulant, fibrinolytic, blood pressure lowering effects and antioxidant activity. Bacillus subtilis natto belongs to the Bacillus subtilis species and it is the basis for the production of traditional Japanese food.Poster: [https://cer.ihtm.bg.ac.rs/handle/123456789/5016

Ex situ bioremediation as an example of circular economy

У нашем раду смо испитивали процес биоремедијације земљишта загађеног мазутом који је потицао из топлане