Contribution to Globularia phylogeny based on nuclear ribosomal spacer and two chloroplast DNA regions

Background and Purpose: Molecular approach has a major impact on phylogenetic studies of plants, considering that it gives useful information about evolutionary events and relations on all taxonomic levels. The sequence data of the nuclear ITS and of two chloroplast regions, trnL-trnF spacer and rbcL gene, obtained from thirteen Globularia L. taxa, including five Anatolian endemics, representing six sections altogether, were analyzed in order to determine the relations between the European and the Anatolian species and get a better insight into the phylogeny of several closely related Globularia taxa.  Materials and Methods: Total cellular DNA was extracted from fresh or frozen leaf tissue of thirteen Globularia samples. The ITS regions of nuclear DNA and two chloroplast DNA regions were amplified and sequenced. Obtained nuclear and combined plastid data matrices were subjected to Maximum Parsimony analyses. Results and Conclusions: Molecular data that were obtained in this study indicate the existence of separate centers of diversification for the European and the Anatolian Globularia. The results provide support for relationships among the studied Anatolian endemic species and indications for a redefinition of affinities of some of the European species. The results presented herein are discussed along with available morphological, karyological, phytogeographical and molecular data. Keywords: Globularia, ITS, trnL-trnF, rbcL, Maximum Parsimony, Quaternary</p

ANXIETY, DEPRESSION AND PERSONALITY TYPES IN PATIENTS WITH INFLAMMATORY BOWEL DISEASE: COMPARISONS WITH PEPTIC ULCER AND THE GENERAL POPULATION

Background: To explore corellation of anxiety, depression ant type of personality in inflammatory bowel disease (IBD) and compare with peptic ulcer (PU). Subjects and methods: In this study, prevalence of anxiety, depression and type of personality was investigated in 362 cases divided into three groups: 112 of IBD patients, 122 of peptic ulcer patients and 128 of control group who didn\u27t have any gastrointestinal or psychic complaints. IBD and peptic ulcer diagnosis were established by standard diagnostic procedures (anamnesis, clinical manifestations, laboratory, endoscopy and biopsy in IBD and upper endoscopy in peptic ulcer). Anxiety and depression were established by Hamilton anxiety rating scale (HAM-A) and Hamilton rating test for depression (HAM-D). Type of A/B personality was established by Bortner scale and D type of personality by Denollet scale (DS14). Results: Anxiety was found in 47 (41.9%) and depression in 44 (38.3%) of a total of 112 IBD cases. In group with peptic ulcer anxiety was found in 40 (32.8%) and depression aalso in 40 (32.8%) of total 122 cases. In control group anxiety was diagnosed in 21 (16.4%) and depression in 20 (15.6%) of total 128 cases. Anxiety and depresson were significantly higher in both groups than in control group but anxiety and depression were significantly higher in IBD group than peptic ulcer group. D type of personality was statistically significant in peptic ulcer group. Conclusion: Anxiety and depression in IBD and peptic ulcer cases have a greater prevalence compared to the normal population and surprisingly are higher in IBD than peptic ulcer group. D type of personality is associated with peptic ulcer

The Cytotoxic Effect of Sodium Hypochlorite on Cell Cultures

Natrij-hipoklorit (NaOCl) najčešće je rabljena otopina za ispiranje i dezinfekciju tijekom obradbe korijenskoga kanala, unatoč činjenici da može iritirati periapeksno tkivo. Svrha ovoga rada bila je utvrditi citotoksičnost vodene otopine NaOCl na humanim (HeLa) stanicama karcinoma grlića maternice i mišjim (L929) fibroblastima. Ispitane su 0,5%, 1,0%, 2,5% i 5,25% koncentracije NaOCl. Ukupni broj stanica izbrojen je elektroničkim brojačem, a broj živih stanica izbrojen je pod svjetlosnim mikroskopom uz dodatak vitalne nigrosin boje. Najmanje promjene morfologije, broja i postotka živih stanica nađene su kod koncentracije 0,5%, a 5,25%-tna koncentracija uzrokovala je potpuni gubitak HeLa i L929 stanica. Utvrđena je statistički vjerodostojna razlika (p<0,05) između ispitivanih koncentracija NaOCl i kontrolne skupine. Između 1,0% i 2,5% NaOCl razlika u toksičnosti nije bila vjerodostojna za obje stanične linije (p<0.05).Sodium hypochlorite (NaOCl) is the most popular irrigation solution used in root canal treatment, in spite of the fact that it may irritate the vital tissues. This study was designed to evaluate the cytotoxic effect of a water solution of sodium hypochlorite on cultures of human cervical carcinoma HeLa cells and mouse L929 fibroblasts by determining the number of vital cells. The tested concentrations of 0.5%, 1.0%, 2.5%, 5.25% were prepared in Eagle\u27s growth medium. The number of cells was counted by electronic counter while the number of viable cells was determined, after the addition of nigrosin dye, by a light microscope. Concentration of 0.5% NaOCl caused the least changes in morphology, number and percentage of viable cells while 5.25% concentration caused total loss of HeLa and L929 cells. All tested concentrations were cytotoxic with significant difference (p<0.05) compared to the control group. However, the results showed that there was no significant difference between 1.0% and 2.5% concentration of NaOCl for both cell lines (p<0.05)

The Cytotoxic Effect of Sodium Hypochlorite on Cell Cultures

Natrij-hipoklorit (NaOCl) najčešće je rabljena otopina za ispiranje i dezinfekciju tijekom obradbe korijenskoga kanala, unatoč činjenici da može iritirati periapeksno tkivo. Svrha ovoga rada bila je utvrditi citotoksičnost vodene otopine NaOCl na humanim (HeLa) stanicama karcinoma grlića maternice i mišjim (L929) fibroblastima. Ispitane su 0,5%, 1,0%, 2,5% i 5,25% koncentracije NaOCl. Ukupni broj stanica izbrojen je elektroničkim brojačem, a broj živih stanica izbrojen je pod svjetlosnim mikroskopom uz dodatak vitalne nigrosin boje. Najmanje promjene morfologije, broja i postotka živih stanica nađene su kod koncentracije 0,5%, a 5,25%-tna koncentracija uzrokovala je potpuni gubitak HeLa i L929 stanica. Utvrđena je statistički vjerodostojna razlika (p<0,05) između ispitivanih koncentracija NaOCl i kontrolne skupine. Između 1,0% i 2,5% NaOCl razlika u toksičnosti nije bila vjerodostojna za obje stanične linije (p<0.05).Sodium hypochlorite (NaOCl) is the most popular irrigation solution used in root canal treatment, in spite of the fact that it may irritate the vital tissues. This study was designed to evaluate the cytotoxic effect of a water solution of sodium hypochlorite on cultures of human cervical carcinoma HeLa cells and mouse L929 fibroblasts by determining the number of vital cells. The tested concentrations of 0.5%, 1.0%, 2.5%, 5.25% were prepared in Eagle\u27s growth medium. The number of cells was counted by electronic counter while the number of viable cells was determined, after the addition of nigrosin dye, by a light microscope. Concentration of 0.5% NaOCl caused the least changes in morphology, number and percentage of viable cells while 5.25% concentration caused total loss of HeLa and L929 cells. All tested concentrations were cytotoxic with significant difference (p<0.05) compared to the control group. However, the results showed that there was no significant difference between 1.0% and 2.5% concentration of NaOCl for both cell lines (p<0.05)

Kroz prostor i vrijeme: Zbornik u čast Miri Menac-Mihalić

Zbornik Kroz prostor i vrijeme posvećen je prof. dr. sc. Miri Menac-Mihalić, dugogodišnjoj profesorici Filozofskog fakulteta Sveučilišta u Zagreba te članici suradnici Hrvatske akademije znanosti i umjetnosti. Prof. dr. sc. Mira Menac-Mihalić iznimno je plodna znanstvenica koja se, uz veliki broj radova iz dijalektologije, smatra i začetnicom hrvatske dijalektne frazeologije. U zborniku posvećenom njezinu radu i djelovanju objavljeni su prilozi koji su grupirani u dvije glavne tematske cjeline (dijalektologija/povijest jezika "O govorima kroz vrijeme" i frazeologija "Živost frazema"). Radovi predstavljaju izniman znanstveni doprinos hrvatskoj filologiji. Velikim su dijelom rezultat terenskih istraživanja kojima se prikupljala građa za obradu. Donose precizne rezultate o fonološkim/morfološkim/leksičkim značajkama hrvatskih mjesnih govora, a istražuju se i frazemi potvrđeni u hrvatskoj nacionalnoj ili dijalektnoj frazeologiji. Dio građe koji se opisuje ekscerpiran je iz postojeće literature te pomno i iscrpno analiziran

Kroz prostor i vrijeme: Zbornik u čast Miri Menac-Mihalić

Zbornik Kroz prostor i vrijeme posvećen je prof. dr. sc. Miri Menac-Mihalić, dugogodišnjoj profesorici Filozofskog fakulteta Sveučilišta u Zagreba te članici suradnici Hrvatske akademije znanosti i umjetnosti. Prof. dr. sc. Mira Menac-Mihalić iznimno je plodna znanstvenica koja se, uz veliki broj radova iz dijalektologije, smatra i začetnicom hrvatske dijalektne frazeologije. U zborniku posvećenom njezinu radu i djelovanju objavljeni su prilozi koji su grupirani u dvije glavne tematske cjeline (dijalektologija/povijest jezika "O govorima kroz vrijeme" i frazeologija "Živost frazema"). Radovi predstavljaju izniman znanstveni doprinos hrvatskoj filologiji. Velikim su dijelom rezultat terenskih istraživanja kojima se prikupljala građa za obradu. Donose precizne rezultate o fonološkim/morfološkim/leksičkim značajkama hrvatskih mjesnih govora, a istražuju se i frazemi potvrđeni u hrvatskoj nacionalnoj ili dijalektnoj frazeologiji. Dio građe koji se opisuje ekscerpiran je iz postojeće literature te pomno i iscrpno analiziran

Prevalence of amebiasis in inflammatory bowel disease in University Clinical Hospital Mostar.

AimTo explore the prevalence of amebiasis in inflammatory bowel disease (IBD), Crohn's disease and ulcerative colitis, in patients in Clinical hospital Mostar (Bosnia and Herzegovina, region of Herzegovina).MethodsIn this study, Entamoeba histolytica/dispar prevalence was investigated in fresh faeces by native microscopy and immunochromatographic rapid assay "RIDA(®)QUICK Entamoeba test", in 119 cases of new found IBD patients, 84 of ulcerative colitis and 35 of Crohn's disease and in control group who had also 119 patients who didn't have any gastrointestinal complaints. IBD diagnosis was established by standard diagnostic procedures (anamnesis, clinical manifestations, laboratory, endoscopy and biopsy).ResultsEntamoeba histolytica/dispar were found in 19 (16.0&nbsp;%) of a total of 119 cases, 12 (14.3&nbsp;%) of the 84 patients with ulcerative colitis and 7 (20.0&nbsp;%) of the 35 patients with Crohn's disease. As for the 119 patients in the control group who had not any gastrointestinal complaints, 2 (1.7&nbsp;%) patients were found to have E. histolytica/dispar in their faeces. Amoeba prevalence in the patient group was determined to be significantly higher in group with Crohn's disease, ulcerative colitis and IBD total than in the control group (p&nbsp;&lt;&nbsp;0.001).ConclusionAmeba infections in patients with Crohn's disease and ulcerative colitis, have a greater prevalence compared to the normal population

ANXIETY, DEPRESSION AND PERSONALITY TYPES IN PATIENTS WITH INFLAMMATORY BOWEL DISEASE: COMPARISONS WITH PEPTIC ULCER AND THE GENERAL POPULATION

Aim: To explore corellation of anxiety, depression ant type of personality in inflammatory bowel disease (IBD) and compare with peptic ulcer (PU). Subjects and methods: In this study, prevalence of anxiety, depression and type of personality was investigated in 362 cases divided into three groups: 112 of IBD patients, 122 of peptic ulcer patients and 128 of control group who didn't have any gastrointestinal or psychic complaints. IBD and peptic ulcer diagnosis were established by standard diagnostic procedures (anamnesis, clinical manifestations, laboratory, endoscopy and biopsy in IBD and upper endoscopy in peptic ulcer). Anxiety and depression were established by Hamilton anxiety rating scale (HAM-A) and Hamilton rating test for depression (HAM-D). Type of A/B personality was established by Bortner scale and D type of personality by Denollet scale (DS14). Results: Anxiety was found in 47 (41.9%) and depression in 44 (38.3%) of a total of 112 IBD cases. In group with peptic ulcer anxiety was found in 40 (32.8%) and depression aalso in 40 (32.8%) of total 122 cases. In control group anxiety was diagnosed in 21 (16.4%) and depression in 20 (15.6%) of total 128 cases. Anxiety and depresson were significantly higher in both groups than in control group but anxiety and depression were significantly higher in IBD group than peptic ulcer group. D type of personality was statistically significant in peptic ulcer group. Conclusion: Anxiety and depression in IBD and peptic ulcer cases have a greater prevalence compared to the normal population and surprisingly are higher in IBD than peptic ulcer group. D type of personality is associated with peptic ulcer