14 research outputs found
The effect of cadmium on ascorbic acid, vitamin E and coenzyme Q concentrations in rat kidneys: A possible protective role of coenzyme Q10
Projekat ministarstva br. 03E18 i 03E2
Effects of cisplatin on lipid peroxidation and the glutathione redox status in the liver of male rats: The protective role of selenium
The role of oxidative stress in cisplatin (CP) toxicity and its prevention by pretreatment with selenium (Se) was investigated. Male Wistar albino rats were injected with a single dose of cisplatin (7.5 mg CP/kg b.m., i.p.) and selenium (6 mg Se/kg b.m, as Na2SeO3, i.p.) alone or in combination. The results suggest that CP intoxication induces oxidative stress and alters the glutathione redox status: reduced glutathione (GSH), oxidized glutathione (GSSG) and the GSH/GSSG ratio (GSH RI), resulting in increased lipid peroxidation (LPO) in rat liver. The pretreatment with selenium prior to CP treatment showed a protective effect against the toxic influence of CP on peroxidation of the membrane lipids and an altering of the glutathione redox status in the liver of rats. From our results we conclude that selenium functions as a potent antioxidant and suggest that it can control CP-induced hepatotoxicity in rats.U naÅ”oj studiji ispitivana je uloga oksidacionog stresa u toksiÄnosti cisplatina (SR) i njegova prevencija pretretmanom selenom (Se). Mužjaci Wistar albino pacova su inicirani jednom dozom cisplatina (7.5 mg CP/kg t.m., i.p.) i selena (6 mg Se/kg t.m, kao Na2SeO3, i.p.) pojedinaÄno ili u kombinaciji. Rezultati pokazuju da intoksikacija SR uzrokuje oksidacioni stres i promenu glutation redoks statusa: redukovanog (GSH), oksidovanog (GSSG) i GSH/GSSG indeksa (GSH RI), kao i poveÄanje lipidne peroksidacije (LPO) u jetri pacova. Tretman Se koji je prethodio tretmanu SR pokazao je zaÅ”titne efekte protiv toksiÄnog delovanja SR na peroksidaciju lipida membrane i promenu glutation redoks statusa u jetri pacova. Na osnovu naÅ”ih rezultata zakljuÄujemo da Se, deluje kao snažan antioksidans i da može imati ulogu u kontroli SR indukovane hepatotoksiÄnosti kod pacova.Projekat ministarstva br. 143035
Time course of hematological parameters in bleeding-induced anemia
In order to investigate daily changes of hematological parameters in bleeding-induced anemia, we treated Wistar albino male rats by daily bleeding (1.5-2 mL of blood from the tail vein for eight days). Blood samples were taken before (on day zero) and on the first to eighth days of bleeding. The values of hematocrit, hemoglobin, and erythrocyte count decreased significantly after the second, sixth, and second days of bleeding, respectively. The number of leukoĀcytes and platelets, as well as Heinz body levels, increased significantly after the third and second days of treatment. The percentage of reticulocytes increased significantly from the second day and attained the maximum level (32.55 Ā± 0.96%) on the eighth day
Concentration of antioxidant compounds and lipid peroxidation in the liver and white muscle of hake (Merluccius merluccius L.) in the Adriatic sea
Specimens of a marine freshwater fish - hake (Merluccius merluccius L.) - were collected at the localities of Platamuni and Valdanos (Montenegro, South Adriatic) in the spring of 2003. Our results showed higher concentrations of LPO, Vit C, and Vit E in the liver in comparison with white muscle. The concentration of LPOin both tissues was higher, while that of Vit E was lower at Valdanos compared to Platamuni. These differences in parameters of oxidative stress are partly due to differences in temperature and the concentrations of nitrites, nitrates, and detergents in the waters of Valdanos compared to Platamuni.Tokom 2003. godine uzorkovane su jedinke morske ribe - osliÄa (Merluccius merluccius L.) iz voda sa lokaliteta Platamuni i Valdanos (Crna Gora, južni Jadran) u proleÄnom periodu. Dobijeni rezultati su pokazali veÄe koncentracije LP, Vit Ci Vit E u jetri osliÄa u odnosu na belo miÅ”iÄno tkivo. Koncentracija LP je bila veÄa, a Vit E manja u oba ispitivana tkiva osliÄa u vodama lokaliteta Valdanos u poreÄenju sa Platamuni. Ovakve promene parametara oksidacionog stresa posledica su promena temperature, kao i poveÄanih koncentracija nitrita, nitrata i deterdženata u vodama lokaliteta Valdanos u odnosu na Platamune.Projekat ministarstva br. 143035
Glutathione status in the blood of rats after reticulocytosis induced by phenylhydrazine and bleeding
In this experiment, we compared the in vivo effects of phenylhydrazine (PHZ) and bleeding treatment on the redox status and glutathione antioxidative mechanism parameters in the plasma and red blood cells (RBC) of rats. Results showed a lower level of reactive oxygen species (ROS), a higher level of lipid peroxidation and the effective antioxidative role of the glutathione system in the blood of bleeding rats. PHZ-treatment induced higher concentrations of ROS and an accumulation of oxidized glutathione in the plasma, while the glutathione system showed a satisfactory antioxidative capacity in the RBC of rats. When comparing the two anemic groups, the PHZ-treated rats showed marked oxidative stress in the plasma.Cilj ovog rada je bio da se isprate in vivo efekti fenilhidrazinom (PHZ) i krvarenjem indukovane retikulocitoze na parametre redoks i glutationskog antioksidativnog statusa u plazmi i crvenim krvnim Äelijama (RBC) pacova. Rezultati pokazuju niži nivo reaktivnih vrsta kiseonika (ROS), viÅ”i nivo lipidne peroksidacije i efikasnu antioksidacionu ulogu glutationskog sistema u krvi iskvavljenih pacova. Tretman PHZom prouzrokovao je viÅ”e koncentracije ROSa i akumulaciju oksidovanog glutationa u plazmi, dok je glutationski sistem pokazao efikasan antioksidativni kapacitet u RBCu pacova. Kada se uporede dve anemiÄne grupe, izraženiji oksidacioni stres se javlja u plazmi pacova tretiranih PHZom.Projekat ministarstva br. 143035
The effect of cadmium on ascorbic acid, vitamin E and coenzyme Q concentrations in rat kidneys: A possible protective role of coenzyme Q10
Projekat ministarstva br. 03E18 i 03E2
Effects of cisplatin on lipid peroxidation and the glutathione redox status in the liver of male rats: The protective role of selenium
The role of oxidative stress in cisplatin (CP) toxicity and its prevention by pretreatment with selenium (Se) was investigated. Male Wistar albino rats were injected with a single dose of cisplatin (7.5 mg CP/kg b.m., i.p.) and selenium (6 mg Se/kg b.m, as Na2SeO3, i.p.) alone or in combination. The results suggest that CP intoxication induces oxidative stress and alters the glutathione redox status: reduced glutathione (GSH), oxidized glutathione (GSSG) and the GSH/GSSG ratio (GSH RI), resulting in increased lipid peroxidation (LPO) in rat liver. The pretreatment with selenium prior to CP treatment showed a protective effect against the toxic influence of CP on peroxidation of the membrane lipids and an altering of the glutathione redox status in the liver of rats. From our results we conclude that selenium functions as a potent antioxidant and suggest that it can control CP-induced hepatotoxicity in rats
Concentration of antioxidant compounds and lipid peroxidation in the liver and white muscle of hake (Merluccius merluccius L.) in the Adriatic sea
Specimens of a marine freshwater fish - hake (Merluccius merluccius L.) - were collected at the localities of Platamuni and Valdanos (Montenegro, South Adriatic) in the spring of 2003. Our results showed higher concentrations of LPO, Vit C, and Vit E in the liver in comparison with white muscle. The concentration of LPOin both tissues was higher, while that of Vit E was lower at Valdanos compared to Platamuni. These differences in parameters of oxidative stress are partly due to differences in temperature and the concentrations of nitrites, nitrates, and detergents in the waters of Valdanos compared to Platamuni.Tokom 2003. godine uzorkovane su jedinke morske ribe - osliÄa (Merluccius merluccius L.) iz voda sa lokaliteta Platamuni i Valdanos (Crna Gora, južni Jadran) u proleÄnom periodu. Dobijeni rezultati su pokazali veÄe koncentracije LP, Vit Ci Vit E u jetri osliÄa u odnosu na belo miÅ”iÄno tkivo. Koncentracija LP je bila veÄa, a Vit E manja u oba ispitivana tkiva osliÄa u vodama lokaliteta Valdanos u poreÄenju sa Platamuni. Ovakve promene parametara oksidacionog stresa posledica su promena temperature, kao i poveÄanih koncentracija nitrita, nitrata i deterdženata u vodama lokaliteta Valdanos u odnosu na Platamune.Projekat ministarstva br. 143035
Time course of hematological parameters in bleeding-induced anemia
In order to investigate daily changes of hematological parameters in bleeding-induced anemia, we treated Wistar albino male rats by daily bleeding (1.5-2 mL of blood from the tail vein for eight days). Blood samples were taken before (on day zero) and on the first to eighth days of bleeding. The values of hematocrit, hemoglobin, and erythrocyte count decreased significantly after the second, sixth, and second days of bleeding, respectively. The number of leukoĀcytes and platelets, as well as Heinz body levels, increased significantly after the third and second days of treatment. The percentage of reticulocytes increased significantly from the second day and attained the maximum level (32.55 Ā± 0.96%) on the eighth day.U cilju ispitivanja dnevnih promena hematoloÅ”kih parametara u toku anemije indukovane dnevnim krvarenjem, tretirali smo Wistar albino pacove dnevnim krvarenjem (1.5-2 ml krvi iz repne vene u toku 8 dana). Uzorci krvi uzimani su pre (0 dan) i 1-8. dana krvarenja. Vrednosti za hematokrit, hemoglobin i broj eritrocita znaÄajno opadaju nakon drugog, Å”estog i drugog dana krvarenja. Broj leukocita i trombocita, kao i nivo formiranja Heinz-ovih telaÅ”aca raste znaÄajno nakon treÄeg i drugog dana tretmana. Procenat retikulocita znaÄajno raste od drugog dana i dostiže maksimalni nivo (32.55 Ā± 0.96 %) osmog dana tremana.Projekat ministarstva br. 143035
Glutathione status in the blood of rats after reticulocytosis induced by phenylhydrazine and bleeding
In this experiment, we compared the in vivo effects of phenylhydrazine (PHZ) and bleeding treatment on the redox status and glutathione antioxidative mechanism parameters in the plasma and red blood cells (RBC) of rats. Results showed a lower level of reactive oxygen species (ROS), a higher level of lipid peroxidation and the effective antioxidative role of the glutathione system in the blood of bleeding rats. PHZ-treatment induced higher concentrations of ROS and an accumulation of oxidized glutathione in the plasma, while the glutathione system showed a satisfactory antioxidative capacity in the RBC of rats. When comparing the two anemic groups, the PHZ-treated rats showed marked oxidative stress in the plasma.Cilj ovog rada je bio da se isprate in vivo efekti fenilhidrazinom (PHZ) i krvarenjem indukovane retikulocitoze na parametre redoks i glutationskog antioksidativnog statusa u plazmi i crvenim krvnim Äelijama (RBC) pacova. Rezultati pokazuju niži nivo reaktivnih vrsta kiseonika (ROS), viÅ”i nivo lipidne peroksidacije i efikasnu antioksidacionu ulogu glutationskog sistema u krvi iskvavljenih pacova. Tretman PHZom prouzrokovao je viÅ”e koncentracije ROSa i akumulaciju oksidovanog glutationa u plazmi, dok je glutationski sistem pokazao efikasan antioksidativni kapacitet u RBCu pacova. Kada se uporede dve anemiÄne grupe, izraženiji oksidacioni stres se javlja u plazmi pacova tretiranih PHZom.Projekat ministarstva br. 143035