Calicophoron daubneyi (Diginea: Paramphiszomidae): Účinnost anthelmintik u přirozeně infikovaného skotu

The effectiveness of anthelmintics in the treatment of paramphistomosis in farm animals in the Czech Republic has not been sufficiently investigated. This study was focused on selected breeds of beef cattle. Identification of Calicophoron daubneyi was performed by sedimentation and verified by PCR using the 5.8S rRNA gene. Animals were treated specifically for C. daubneyi infection only. Totally 400 positive animals were included in the study. The anthelmintic Closamectin (Closantel/Ivermectin) was evaluated as the most effective veterinary medicinal product with an efficiency of 80%, followed by Distocur (Oxyclozanid) with an efficiency of 78%, Aldifal (Albendazol) with an efficiency of 71% and Levatum Plus (Ivermectin/Clorsulon) with an efficiency of 62%.Účinnost anthelmintik při léčbě paramfistózy u hospodářských zvířat v České republice není dostatečně prozkoumána. Tato studie byla zaměřena na vybraná plemena masného skotu. Identifikace Calicophoron daubneyi byla provedena sedimentací a ověřena pomocí PCR s využitím genu 5,8S rRNA. Zvířata byla léčena specificky pouze na infekci C. daubneyi. Do studie bylo zařazeno celkem 400 pozitivních zvířat. Jako nejúčinnější veterinární léčivý přípravek bylo vyhodnoceno anthelmintikum Closamectin (Closantel/Ivermectin) s účinností 80 %, následované Distocurem (Oxyclozanid) s účinností 78 %, Aldifalem (Albendazol) s účinností 71 % a Levatum Plus (Ivermectin/Clorsulon) s účinností 62 %

Analysis of election programs of political parties in 2010 in the field of direct taxation

The purpose of my bachelor's degree work has been evaluation of chosen electioneering programs of political parties. From separate programs information were used, according to which income range and tax yield of individuals as well as corporations has been calculated to meet this objective. This analysis has showed that KSCM and ODS would bring the most as for taxation of individuals and corporations. There were the biggest differences evident among programs for high income tax payers on income tax from dependent work. In case of corporations, companies have earned the highest net profit according to the electioneering programs of ODS

Common consolidated corporate tax base in the EU

The aim of my thesis is through comparative analysis find the differences between the newly proposed CCCTB directive and corporate income tax systems of France, Germany and Great Britain. The work is mainly focused on the comparison method of depreciation, access to inventory, the amount of corporate tax and transfer of tax losses. Although each system has its tax certain specifics, one would assume that the authors of the proposed directive let themselves be inspired by the strong states of the European Union. However, the final chapter of my thesis shows, that the majority of monitored items was not affected by these states

Evaluation meat quality in depading on condition of transfer

Work deals with transport of animals for slaughter and its influence on the quality of meat. Summarizes the right ways of loading, unloading and manipulating with animals. Determinates the precise requirements for the means of transport and transport equipment. Includes the most used transportation by road and marginally used transport by rail and water. Mentions the most common causes of injuries, deaths and losses during transport. Points to the influence of manipulations with animals on the quality of the meat. The result of improper treatment of animals are meat defects DFD and PSE, witch are commonly caused by stress and exhaustion of energy reserves. Describes the basic defects of meat of slaughtered animals-bovine animals and pigs. Mentions the basic laboratory techniques for the detection of these abnormalities

Analysis and implementation of BI applications for a wholesale company

Hlavním cílem bakalářské práce je navrhnout a implementovat business intelligence pro vybranou velkoobchodní společnost. V první části práce je nejdříve charakterizován trh s plasty, na němž společnost působí. Následuje její analýza, zaměřující se na organizační strukturu, informační systém a důležité standardní a klíčové oblasti řízení, které mohou být potenciálně předmětem výsledného business intelligence řešení. Na analýzu společnosti navazuje sběr jejích požadavků a samotný hrubý návrh business intelligence, který využívá metody dimenzionálního a datového modelování. V poslední části práce dochází k dovršení hlavního cíle, tím je myšlen návrh finálního business intelligence řešení a jeho implementace v rámci společnosti. Výsledkem bakalářské práce jsou čtyři dashboardy konkrétních oblastí řízení společnosti v Power BI, ukazující důležité ukazatele, které vzešly z analýzy společnosti a jejich požadavků. Dashboardy mohou sloužit ke zlepšení výkonnosti a efektivnosti jednotlivých oblastí řízení, stejně tak jako k řízení celé společnosti. Na druhou stranu můžou pomoci k předcházení problémů spojenými s konkrétními oblastmi.The main objective of the Bachelor thesis is to design and implement business intelligence for a selected wholesale company. The plastics market, where operates the company, is characterized in the first part of the thesis. This is followed by company analysis, focusing on the organizational structure, information system and important standard and key management areas which can potentially be the subject of the resulting business intelligence solution. The analysis of the company is followed by the collection of its requirements and the actual rough design of the business intelligence, which uses dimensional and data modelling methods. In the last part of the thesis, the main objective is completed, meaning the design of the final business intelligence solution and its implementation within the company. Four dashboards of specific areas of the company’s management in Power BI are the result of the Bachelor thesis. They show important indicators that emerged from the analysis of the company and its requirements. The dashboards can be used to improve the performance and efficiency of individual management areas as well as to manage the entire company. On the other hand, they can help to prevent problems associated with specific areas

Diversity of Cryptosporidium spp. infecting rodents from genus Apodemus in the Czech Republic

We investigated the species and genotypes of Cryptosporidium infecting wild rodents from the genus Apodemus in ten areas in the year 2012 in the Czech Republic. A total of 207 faecal samples, 182 samples of Apodemus flavicollis and 25 of Apodemus sylvaticus, were screened for presence of Cryptosporidium spp. using both the aniline-carbol-methyl violet staining method and molecular tools. Microscopy examination revealed the presence of Cryptosporidium spp. oocyst in 24 samples of Apodemus flavicollis and 1 sample of Apodemus sylvaticus. Genomic DNA was isolated from both microscopy positive and negative samples. Using the nested PCR amplifying gene encoding the small ribosomal subunit (SSU rRNA) 25 samples were detected positive for the presence of Cryptosporidium-specific DNA. The same results were obtained also in the nested PCR amplifying gene encoding actin. All microscopy positive samples were also PCR positive. Only 19 samples were successfully sequenced, following phylogeny analyses showed presence of two new genotypes. First genotype is phylogenetically related to Cryptosporidium ubiquitum (1 sample) and the second genotype (consisting of several subgroups) related to C. canis (18 samples). The new genotypes seem to be host specific, however this hypothesis needs to be verified using experimental infection in the future. This is the first report of these Cryptosporidium genotypes in Apodemus spp. and for the first time ever

The Current Molecular Epidemiological Scenario of Cryptosporidium, Giardia and Blastocystis in Spain. Implication for Public Health

The enteric protozoan parasites Cryptosporidium spp. and Giardia duodenalis are major contributors to the burden of gastrointestinal diseases globally. Both pathogens primarily affect children living in resource-poor settings with limited or no access to clean water and sanitation facilities, but are also significant public health threats in developed countries. Additionally, Cryptosporidium spp. and G. duodenalis are common causes of waterborne and foodborne outbreaks of gastrointestinal disease globally. Besides, the Stramenopile Blastocystis sp. is the most common eukaryotic organism reported in the human gut. Although its pathogenicity is a topic of debate, there is increasing evidence demonstrating that this protist can be associated with gastrointestinal disorders (diarrhoea, irritable bowel syndrome) and extra-intestinal manifestations, including urticaria. Because Cryptosporidium spp., G. duodenalis and Blastocystis sp. share the same transmission (faecal-oral) route, are able to infect a wide range of animal species other than humans with variable host specificities, and their infective forms are environmentally resilient, the study of these pathogens should be ideally approached under the One Health umbrella. In this context, molecular-based methods including PCR and sequencing provide powerful tools to investigate the epidemiology and transmission of these parasites. In Spain, cryptosporidiosis and giardiosis, but not blastocystosis, are notifiable diseases. However, the true incidence of these infections remain largely unknown because underdiagnosing and underreporting. Symptomatic cryptosporidiosis and giardiosis disproportionally affect children under four years of age, but we know now that subclinical infections are also common in apparently healthy individuals of all age groups. However, molecular data regarding the frequency and diversity of these pathogens are limited and spatially and temporally discontinuous. This chapter aims to provide, from a public veterinary health perspective, an updated account on the epidemiology of Cryptosporidium, G. duodenalis and Blastocystis in Spain, with an emphasis on the description of the species/genotypes circulating in symptomatic and asymptomatic human populations. Current knowledge on the presence of these pathogens in production (livestock), companion (dogs and cats) and wildlife animal species is also discussed, including their potential role as natural reservoirs of human infections, and the available evidence of zoonotic (and anthroponotic) transmission events.Research summarized in this chapter and conducted at the Spanish National Centre for Microbiology was funded by the Health Institute Carlos III (ISCIII), Ministry of Economy and Competitiveness (Spain), under projects CP12/03081 and PI16CIII/00024. The funder had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.S