Effect of Fibroblast Growth Factor (FGF) on Some Serum Oxidative Parameters in Hyperglycemic Rats

Wound healing is a complex and dynamic process that includes multiple biological pathways and has some successive healing periods. Most growth factor is responsible for wound healing. Fibroblast growth factor has a positive effect on wound healing problems that can be caused by diabetes. In the present study, we aimed to investigate exogenous effect bFGF supplementation on serum TBARS, RSH and NOx levels in hyperglysemic rats. Experiments were performed on 30 male Wistar albino rats (weight range: 200-250 g ). Rats were hyperglycemic with STZ (ip, 60 mg/kg). The experimental groups were divided into untreated and bFGF-treated subgroups. bGF was applied locally to the dorsalateral wounds of rats (10 ng/ml). After these administrations, on the 3th and 7th days of wound healing, the animals were sacrificed. Serum TBARS, RSH and NOx levels were recorded spectrophotometrically. The results were expressed as mean ± Standard deviation and the mean differences were compared by Anova Variance Analysis (p lt;0,05). When compared with the treatment group, on the 7th day and the 3rd day, it was found that the serum TBARS levels increased statistically in hyperglycemic rats(p lt;0,05). Both in the 3rd day of the untreated and 3rd day of the rats treated with bFGF may significant decrease in the serum RSH levels. bFGF application was found both enhancing and reducing effects on oxidative stres. In subsequent studies, the effect of bFGF, which has positive effects on diabetic wound healing, on oxidative events can be investigated in detail using different doses and different treatment periods

Erratum to: Some Serum Oxidative Parameters in Normoglycemic Rats: Vascular Endothelial Growth Factor (VEGF) Application

CAUSE OF ERRATUM After publication of this work [E1], it has come to our attention that there is a typographical error in the section of Material and Methods. Immediately, we requested from the journal for the correction of the error as follows. MATERIAL AND METHODS ….. Local Ethics Committee for Animal Experiments (G.Ü. ET-10.118)

Supplementation of Thymoquinone and Carob Together in the Experimental Rat Asthma Model: Oxidative Effect on the Liver Tissue

Asthma, an important public health problem, is a common, potentially serious, medical condition in children, adults and pregnant women. The aim of this study is to investigate the effects of the combined use of thymoquinone and carob on liver tissue oxidative events, following the experimental asthma model. 18 male albino wistar rats were divided into 3 groups as: the control group, the experimental asthma group and treated group (A+TQ+C). In the asthmatic groups, ovalbumin and alum were given intraperitoneally on the 0 and 14th days, and sensitized by inhalation on the 21st, 22nd and 23rd days. In the next 5 days, thymoquinone and carob were given to the group to be treated by intragastric gavage method. In all experimental groups, glutathione (GSH), ascorbic acid (AA), malondialdehyde (MDA) and nitric oxide (NOx) levels were measured spectrophotometrically to evaluate the oxidant-antioxidant status in the liver tissue of rats. While liver tissue GSH and AA levels increased, NOx levels were found to decrease following thymoquinone and carob administration in the treated group (A+TQ+C) when compared other groups (Control and Asthma). However, MDA levels, which are the indicator of lipid peroxidation, were found to be statistically significantly increased in the treated group (A+TQ+C) (

Dynamic thiol/disulphide balance in patients undergoing hypotensive anesthesia in elective septoplasties

Objective We aimed to investigate the effects of hypotensive anaesthesia on oxidative stress with serum thiol/disulphide balance in patients undergoing elective septoplasty procedures under general anaesthesia. Methods Seventy-two patients between the ages of 18-60, with a physical condition I -II, according to the American Society of Anesthesiologists, were included in this prospective observational study. Septoplasty was chosen for standard surgical stress. According to the maintenance of anaesthesia, patients were divided into the groups as Hypotensive Anaesthesia (n = 40) and Normotensive Anaesthesia (n = 32). Serum thiol/disulphide levels were measured by the method developed by Erel & Neselioglu. Results The native thiol and total thiol values of both groups measured at the 60th min intraoperatively were significantly lower than the preoperative values (both P 40 years and female gender were found to have a significant effect on dynamic oxidative stress (P = .002 and .001, respectively). Conclusion This pilot study has found that hypotensive anaesthesia had no adverse effect on dynamic thiol/disulphide balance in elective surgeries

Potential of morin and hesperidin in the prevention of cisplatin-induced nephrotoxicity

COSKUN CEVHER, SULE/0000-0001-6204-2845WOS: 000383902000020PubMed: 27425870Oxidative stress is one of the important mechanisms of cisplatin-induced nephrotoxicity. Therefore, this study was designed to explore the potential protective effects of morin and/or hesperidin on oxidative stress in cisplatin-induced nephrotoxicity. This study was performed on 42 Wistar rats. Rats were divided into seven groups: control, morin, hesperidin, cisplatin, cisplatin+morin, cisplatin+hesperidin, and cisplatin+morin+hesperidin. Morin and/or hesperidin were given for 10 consecutive days by oral gavage and on the 4th day a single dose of cisplatin (7mg/kg) was injected intraperitoneally. After administrations, on the 11th day of the experiment the animals were killed, and malondialdehyde (MDA), nitric oxide (NOx), glutathione (GSH) levels and myeloperoxidase (MPO), catalase (CAT), superoxide dismutase (SOD) activity were measured. Cisplatin-treated rats showed increased levels of MDA, and decreased levels of NOx also activity of CAT. Morin and/or hesperidin pretreatment prevent oxidative stress in kidney tissue, while they increase the NOx level, CAT activity, and decrease MPO activity. In conclusion, morin+hesperidin pretreatment may have a significant potential for protection of cisplatin-induced nephrotoxicity

Isatis Glauca Subsp. Sivasica Extract Contributes to Diabetic Wound Healing Process via Increased Collagen and Nitric Oxide Content

Isatis L. genus highly endemic and that is traditionally suggested for wound management in Turkey. In this study, we have reported the effect of I. glauca subsp. sivasica extract on diabetic wound healing process. A diabetic model was generated in Wistar rats using streptozotocin injection. Rats were divided into two main groups: control group and Isatis group. Full thickness excisional skin wounds were created by using a biopsy punch. The Isatis group was treated with single daily dose I. glauca subsp. sivasica extract (50 mg/kg). Rats were sacrificed day 3 or 7 after wounding. The dominant phenolic compounds identified with RP-HPLC-DAD from Isatis extract were the benzoic acid and vanillic acid. Isatis extract significantly accelerated wound healing process considering the wound closure rates (WCR). Moreover, the levels of collagen and nitric oxide were elevated on days 3 and 7 by Isatis administration in the diabetic wound tissue. These data suggest that, for the first time, I. glauca subsp. sivasica extract may have the potential to promote the impaired wound healing in patients with diabetes

Comparison of NOx and TBARs Levels during Wound Healing in Normal and Hyperglycemia Rats

WOS: 000360810100133