Effects of Different Applications on Postoperative Seroma Formation and Wound Healing Following Mastectomy and Axillary Dissection in Rats

The most frequent postoperative complication after breast surgery is seroma formation. Seroma occurs due to lymphatic and vascular fluid leakage into the dead space created by surgical dissection. The objective of the research was to evaluate the effects of local fibrin glue, tetracycline, talc applications, and flap fixation technique on reducing seroma formation after mastectomy and axillary dissection. In addition, we aimed to determine the level of efficacy for these applications, as well as to identify the most appropriate method to be used in operations with high risk of seroma formation. Materials and Methods. This experimental study was conducted using a total of 60 female Wistar albino rats. They were allocated into six groups and each comprised ten rats. Unilateral mastectomy and axillary dissection were performed on all the rats. Local applications of fibrin glue, tetracycline, talc, and alcoholic iodine were performed in four separate groups. Flap fixation technique was applied in one group and those rats that did not receive any intervention constituted the control group. On the 10th postoperative day, seroma was aspirated under anesthesia, and the amount of seroma fluid was recorded. Seroma fluid was analyzed for interleukin 1-β, vascular endothelial growth factor, and C-reactive protein levels. Tissue samples were obtained from the skin overlaying the dissection area, the axilla, and the thoracic wall. Wound healing was evaluated with histopathological examination. Results. Seroma volume was lower and the wound healing scores were the highest in the flap fixation group and the tetracycline group as compared to the control group. However, the alcoholic iodine group and the talc group had a greater amount of seroma (p < 0.05). There was no difference between the fibrin glue group and the control group. Conclusions. In our mastectomy model, local application of alcoholic iodine and talc substances caused more wound site problems and postoperative seroma formation. While fibrin glue did not cause wound site problems, it did increase seroma formation. These three substances were determined to be inefficacious in postoperative seroma formation. Local tetracycline application and flap fixation technique were found to reduce postoperative seroma and benefit wound healing

Voltammetric Determination of Epinephrine in Pharmaceutical Sample with a Tyrosinase Nanobiosensor

A novel carbon paste electrode modified with a multiwalled carbon nanotube (MWCNT), tyrosinase, and Nafion membrane (CP/MWCNT/Tyr/Nafion) was developed for voltammetric determination of epinephrine (EP). The CP/MWCNT/Tyr/Nafion biosensor exhibited linear dynamic range from 5.0×10-6 M to 5.0×10-4 M EP concentration with a good correlation coefficient (R2=0.9985). The detection limit of the biosensor was calculated as 3.0×10-7 M EP from the signal-to-noise ratio (S/N = 3). Reproducibility of the biosensor was also calculated from relative standard deviation as 3.8% (n=5). Ascorbic acid (AA) and uric acid (UA) did not interfere in the quantification of epinephrine. The developed biosensor was also successfully applied for the determination of epinephrine in pharmaceutical sample. The CP/MWCNT/Tyr/Nafion biosensor has good sensitivity, selectivity, stability, easy preparation procedure, and short analysis time and can be used for the determination of EP in pharmacological samples

Seroprevalence of Cytomegalovirus Among Pregnant Women of Rize Region

Amaç: Sitomegalovirus (CMV) Herpesviridae ailesinin Beta herpes virinae alt ailesinden bir DNA vi- rüsüdür. CMV enfeksiyonu yaygın bir enfeksiyondur ve gebelikte geçirilmesi gelişmekte olan fetüs için ciddi zararlar meydana getirebilir. Bu çalışmada hastanemiz gebe polikliniğine başvuran gebe kadınlarda CMV’nin IgM ve IgG tipi antikorlarının seroprevalanslarının tespit edilmesi amaçlanmış- tır. Literatürde, Rize yöresinde bu çalışmayla ilişkili veri bulunmamaktadır Materyal ve Metod: Bu çalışmada hastanemiz gebe polikliniğine 2009-2014 tarihleri arasında başvuran 874 gebenin CMV IgM ve 210 gebenin CMV IgG antikor değerleri retrospektif olarak incelendi. Bulgular: CMV IgG ölçümüne göre olguların %0,5’inin (n=1) CMV IgG sonucu negatif, %99,5’inin (n=209) CMV IgG sonucu pozitif olarak saptanmıştır. CMV IgM ölçümüne göre olguların %96,9’unun (n=847) CMV IgM sonucu negatif, %0,5’inin (n=4) ara değer ve %2,6’sının (n=23) CMV IgM sonucu pozitif olarak saptanmıştır. Sonuç: Bu çalışma ile Rize ilinde 5 yıllık periyotta gebe polikliniğine başvuran gebe kadınlardaki CMV seroprevalansı belirlenmiştir.Aim: Cytomegalovirus (CMV) is a DNA virus of the Beta herpes virinae Subfamily And Herpes viridae Family. CMV infection is a common viral infection in pregnancy and may have severe consequences for the developing fetus. In the present study we aimed to investigate seroprevalance of CMV Ig M and Ig G among pregnant women who were admitted to our obstetric outpatient clinic. To the best of our knowledge there has been no data in the literature of this subject in Rize region. Materials and Methods: In this retrospective study, 874 pregnant women were investigated for CMV IgM antibodies and 210 pregnant women were investigated for CMV IgG antibodies, who were admitted to our obstetric outpatient clinic between 2009-2014 Results: CCMV IgG results of the patients were: 0,5% (n=1) negative, 99,5% (n=209) positive. CMV IgM results of the patients were: 96,9% (n=847) negative, 0,5% (n=4) gray zone and 2,6% (n=23) positive. Conclusion: In the present study seroprevalance of CMV was detected among pregnant women who were admitted to our hospital for a 5-year period in Rize regio

Seroprevalence of Toxoplasma gondii among pregnant women admitted to an university hospital

Amaç: Toksoplazmoz, insanları da içeren sıcakkanlı hayvanları enfekte eden zorunlu hücre içi paraziti olan Toxoplasma gondii’nin neden olduğu zoonotik karakterli bir hastalıktır. Bu çalışmada Rize’de üniversite hastanesine başvuran gebe kadınlarda T. gondii seroprevalansının tespit edilmesi amaçlanmıştır. Gereç ve Yöntem: Bu retrospektif çalışmada, hastanemiz gebe polikliniğine 31.07.2009 ile 01.08.2014 tarihleri arasında başvuran 1046 gebenin anti-T. gondii IgM ve 325 gebenin anti-T. gondii IgG antikor değerleri incelendi. Bulgular: Anti-T. gondii IgG ölçümüne göre olguların %55,5’inin sonucu negatif, %3.4’ünün ara değer ve %41.1’inin sonucu pozitif olarak saptanmıştır. Anti-T. gondii IgM ölçümüne göre olguların %94.6’sının negatif, %1.1’inin ara değer ve %4.3’ünün sonucu pozitif olarak saptanmıştır. Sonuç: Gebe kadınlarda T. gondii seropozitiflik oranları ilimizde diğer illerin ortalamalarının hafif üzerindedir. Özellikle gebe ve doğurganlık çağındaki kadınlara bu enfeksiyondan korunma yolları hakkında eğitim verilmesinin önemli ve gerekli olduğunu düşünmekteyiz.Aim: Toxoplasmosis is a zoonosis caused by the obligate intracellular parasite Toxoplasma gondii, which infects warm-blooded animals, including human beings. In this study, we aimed to determine the seroprevalence of T. gondii among pregnant women admitted to an university hospital in Rize. Materials and Methods: In this retrospective study, 1046 pregnant women admitted to our obstetric outpatient clinic between 31.07.2009 and 01.08.2014, were investigated for anti-T. gondii IgM antibody and 325 pregnant women were investigated for anti-T. gondii IgG antibody. Results: Anti-T. gondii IgG results of the patients were found as negative in 55,5%, gray zone in 3,4% and positive in 41,1%. Anti-T. gondii IgM results of the patients were 94,6% negative, 1,1% gray zone and 4,3% positive. Conclusion: The seropositivity of T. gondii is slightly higher among pregnant women in our city than other cities. We think that it is important and necessary to give education to pregnant and fertile women about protection ways of this infectio

Seroprevalence of Rubella Virus among Pregnant Women in Eastern Black Sea Region

Amaç: Rubella virus Togaviridae familyasının Rubivirus genusunun tek üyesidir. Zarflıdır ve tek sarmallı ribo nükleik asit genomuna sahiptir. Bu viruse ilk trimesterde maruz kalan annelerin doğmamış çocukları konjenital rubella sendromu açısından yüksek risk altındadırlar. Bu çalışmada hastanemiz gebe polikliniklerine başvuran gebelerde rubella antikorlarının sıklığının tespit edilmesi amaçlandı. Gereç ve Yöntem: Bu çalışmada hastanemiz gebe polikliniğine 31.07.2009 ile 01.08.2014 tarihleri arasında başvuran 1037 gebenin rubella IgM ve 424 gebenin rubella IgG antikor değerleri retrospektif olarak incelendi. Bulgular: Rubella IgG ölçümüne göre olguların %1,9'unun (n=8) rubella IgG sonucu negatif, %4,2'sinin (n=18) ara değer ve %93,9'unun (n=398) rubella IgG sonucu pozitif olarak saptandı. Rubella IgM ölçümüne göre olguların %99,2'sinin (n=1029) rubella IgM sonucu negatif, %0,5'inin (n=5) ara değer ve %0,3'ünün (n=3) rubella IgM sonucu pozitif olarak saptandı. Sonuç: Bu çalışma ile yöremizdeki gebe kadınlarda 5 yıllık dönemde rubella virüs seroprevalansı tespit edildi. Bölgemizde rubella IgG seroprevalansı oldukça yüksektir ve bu sebeple gebelerde rutin olarak taranmasının gerekli olmadığını düşünüyoruz. Rubella IgM seropozitivitesi ise oldukça düşüktür ve bu sebeple de bu bölgede rutin rubella IgM taraması yapmadan önce obstetrisyenlerin maliyet etkinlik açısından değerlendirme yapmaları gerekirObjective: Rubella virus, a member of the Togaviridae family is the sole member of the genus Rubivirus. It is enveloped and has a single stranded ribonucleicacid genome. Infection acquired in the first trimester of pregnancy poses high risk of congenital rubella syndrome to the unborn child. In the present study we aimed to investigate seroprevalance of rubella antibodies among pregnant women who were admitted to our obstetrics department. Materials and Methods: In this retrospective study, 1037 pregnant women were investigated for rubella IgM antibody and 424 for rubella IgG antibody, who were admitted to our obstetric clinic between 31st July 2009 and 1st August 2014. Results: Rubella IgG results of the patients were; 1,9% (n=8) negative, 4,2% (n=18) grayzone and 93,9% (n=398) positive. Rubella IgM results of the patiences were; 99,2% (n=1029) negative, 0,5% (n=5) grayzone and 0,3% (n=3) positive. Conclusion: As a result, in this study we identified the last 5 years seroprevalance of rubella virus in our region. Seropositivity of rubella virus is high in our region and we think routine screening for rubella IgG is not necessary. Seropositivity rubella IgM is very low in our region. Therefore, obstetricians should reconsider the cost-effectiveness of routine screening for rubella Ig

Primary Tumor Surgery in Patients with De Novo Bone-only Stage IVA/B Breast Cancer: MetS: Protocol MF22-03, Combination Data Analysis of Prospective Studies

24th Annual Meeting of The American Society of Breast Surgeons (ASBrS) -- APR 26-30, 2023 -- MS --[No abstract available

Track density imaging using diffusion tensor imaging data from 1.5 T MRI scanner

Superresolution track density imaging (TDI) has recently been developed for achieving high resolution track density maps from low-resolution diffusion images acquired at 3 T. But, the utility of the approach is still unclear when applied to diffusion tensor imaging (DTI) data acquired at lower 1.5 T magnetic field strength and thus its advantages or disadvantages awaits for exploration. We implemented an algorithm to generate track density maps of human white matter using streamline tracking and tested its performance with data acquired from two healthy volunteers at 1.5 Tesla. The effects of number of diffusion directions and seed selections on the quality of the reconstructed TDI maps were investigated under a variety of settings. The results were visually evaluated by an anatomist and a radiologist, and statistically characterized using gray level cooccurance Matrices (GLCM). Producing high-quality maps with improved resolution required increasing the number of seeds per voxel. Statistical implications were consistent with visual inspection. Low signal-to-noise ratio in DTI data intrinsically yielded low SNR in the final TD map. Accurately defining the diffusion and thus fiber orientation within a voxel necessitated increasing the number of diffusion encoding directions. Our data suggests that TDI image with DTI data acquired at 1.5 T is possible using right trade-offs in data acquisition and processing and has the capability of delineating the substructures of the brain