Flutter calculation based on gvt-results and theoretical mass model

Ground vibration tests (GVT) are a typical source of data for flutter prediction. In this paper, a simple, lowcost method to calculate flutter is presented. In this method, measured frequencies, mode shapes of an airplane are used and, additionally, the theoretical mass model of it. If the theoretical mass model is used, it is possible to calculate generalized masses of modes and cross mass couplings between them. The mass couplings of normal modes should be zero. Orthogonalization is correction of the mode shapes to lead the couplings to zero. The possible orthogonalization methods are presented in chapter 2. Based on eigenmodes of airplane configuration during GVT, it is possible to determine the eigenmodes of the same free airplane after a relatively small mass change, i.e. for another mass distribution that was not investigated by GVT. In the procedure presented in chapter 3, it is assumed that geometric and stiffness properties do not change. The methodology was used in the own flutter calculation software that is useful for flutter prediction of light airplanes and sailplanes. Santrauka Dažnuminiai bandymai žemėje yra tipinis informacijos šaltinis flaterio skaičiavimui. Šiame straipsnyje pateikiamas paprastas ir pigus flaterio skaičiavimo metodas. Šiame metode naudojamos lėktuvo išmatuotų dažnuminių modų formos ir teorinis lėktuvo masių modelis. Naudojant teorinį masių modelį galima apskaičiuoti apibendrintas modų mases ir masių ryšius tarp jų. Normalinių modų masių ryšys turi būti lygus nuliui. Ortogonalizavimu koreguojamos modų formos, siekiant ryšius sumažinti iki nulio. Galimi ortogonalizavimo metodai pateikti antrame skyriuje. Remiantis lėktuvo laisvųjų svyravimo modomis, gautomis dažnuminių bandymų žemėje metu, galima nustatyti kitokio masių pasiskirstymo įtaką laisvųjų svyravimų modoms. Procedūroje, pateiktoje trečiame skyriuje, manoma, kad geometrinės ir standumo savybės nesikeičia. Ši metodologija buvo panaudota savoje programinėje įrangoje flateriui skaičiuoti, kurią galima naudoti lengvų lėktuvų ir sklandytuvų flaterio skaičiavimui. First Publish Online: 14 Oct 2010 Reikšminiai žodžiai: aerodinaminis flateris, dažnuminiai bandymai žemėje, normalinės modos, masių modelis, ortogonalizavimas, masių išsidėstymo pokytis

The ovary of Tubifex tubifex (Clitellata, Naididae, Tubificinae) Is composed of one, huge germ-line cyst that is enriched with cytoskeletal components

Recent studies on the ovary organization and oogenesis in Tubificinae have revealed that their ovaries are small polarized structures that are composed of germ cells in subsequent stages of oogenesis that are associated with somatic cells. In syncytial cysts, as a rule, each germ cell is connected to the central cytoplasmic mass, the cytophore, via only one stable intercellular bridge (ring canal). In this paper we present detailed data about the composition of germ-line cysts in Tubifex tubifex with special emphasis on the occurrence and distribution of the cytoskeletal elements. Using fixed material and live cell imaging techniques, we found that the entire ovary of T. tubifex is composed of only one, huge multicellular germ-line cyst, which may contain up to 2,600 cells. Its architecture is broadly similar to the cysts that are found in other clitellate annelids, i.e. a common, anuclear cytoplasmic mass in the center of the cyst and germ cells that are connected to it via intercellular bridges. The cytophore in the T. tubifex cyst extends along the long axis of the ovary in the form of elongated and branched cytoplasmic strands. Rhodamine-coupled phalloidin staining revealed that the prominent strands of actin filaments occur inside the cytophore. Similar to the cytophore, F-actin strands are branched and they are especially well developed in the middle and outermost parts of the ovary. Microfilaments are also present in the ring canals that connect the germ cells with the cytophore in the narrow end of the ovary. Using Tubulin-Tracker, we found that the microtubules form a prominent network of loosely and evenly distributed tubules inside the cytophore as well as in every germ cell. The well-developed cytoskeletal elements in T. tubifex ovary seem to ensure the integrity of such a huge germline cyst of complex (germ cells - ring canals - cytophore) organization. A comparison between the cysts that are described here and other well-known female germ-line cysts is also made

Fine structure of the midgut epithelium of nicoletia phytophila gervais, 1844 (Zygentoma: Nicoletiidae: Nicoletiinae) with special emphasis on its degeneration

The midgut epithelium of Nicoletia phytophila is composed of columnar digestive cells and regenerative cells that form regenerative nests. The cytoplasm of midgut epithelial cells shows typical regionalization in organelle distribution. Two types of regenerative cells havebeen distinguished: cells which are able to divide intensively and cells which differentiate. Spot desmosomes have been observed between neighboring regenerative cells. The occurrence of intercellular junctions is discussed. Themidgut epitheliumdegenerates both in an apoptotic and necrotic way. Necrosis proceeds during each molting period (cyclic manner), while apoptosis occurs between each molting, when the midgut epithelium is responsible for e.g. digestion. These processes of epithelium degeneration are described at the ultrastructural level. Our studies not only add new information about fine structure of the midgut epithelium of N. phytophila, but contribute to resolving the relationships within the Zygentoma. There are no doubts about the very close sister position of Nicoletiidae and Ateluridae. The midgut epithelium characters confirm their close relationship. However we do not recommend classifying the atelurid genera only within Nicoletiidae: Nicoletiinae

Organ and tissue-specific localisation of selected cell wall epitopes in the zygotic embryo of Brachypodium distachyon

The plant cell wall shows a great diversity regarding its chemical composition, which may vary significantly even during different developmental stages. In this study, we analysed the distribution of several cell wall epitopes in embryos of Brachypodium distachyon (Brachypodium). We also described the variations in the nucleus shape and the number of nucleoli that occurred in some embryo cells. The use of transmission electron microscopy, and histological and immunolocalisation techniques permitted the distribution of selected arabinogalactan proteins, extensins, pectins, and hemicelluloses on the embryo surface, internal cell compartments, and in the context of the cell wall ultrastructure to be demonstrated. We revealed that the majority of arabinogalactan proteins and extensins were distributed on the cell surface and that pectins were the main component of the seed coat and other parts, such as the mesocotyl cell walls and the radicula. Hemicelluloses were localised in the cell wall and outside of the radicula protodermis, respectively. The specific arrangement of those components may indicate their significance during embryo development and seed germination, thus suggesting the importance of their protective functions. Despite the differences in the cell wall composition, we found that some of the antibodies can be used as markers to identify specific cells and the parts of the developing Brachypodium embryo

Neutron diffraction and magnetocaloric effect studies of MnFe 1-x Co x P series of solid solutions

International audienceMnFe 1-x Co x P intermetallic series of solid solutions (0.4<x<0.6) have been studied by means of powder neutron diffraction in 10−320 K temperature range. Rietveld analysis pointed out that Co 2 P-type orthorhombic crystal structure (SG: Pnma) presents for all series. Helicoidal incommensurate antiferromagnetic structure with propagation vector q = [0, 0, q] were evidenced for all compounds at low temperature range. The q value decreases with cobalt content and the second order polynomial q(x) it was evidenced, that is found well correlated with this dependence. Magnetic moments values of µ Mn =3.34 µ B and µ (Fe,Co) =0.06 µ B were determined from neutron diffraction refinements for x=0.4 at 10 K. In addition, magnetic interactions in relations with electronic band structure calculations of MnFe 1-x Co x P were presented and discussed reference to previous published data. Finally, magnetocaloric properties for selected compounds of the MnFe 1-x Co x P and MnFe 0.45 Co 0.45 P 0.9 Ge 0.1 series of compounds are presented

5-Azacitidine induces cell death in a tissue culture of Brachypodium distachyon

Morphological and histological observations revealed that, at a concentration of 50 µM, 5-azacitidine (5-azaC) totally inhibited the induction of embryogenic masses (EM), while the cultivation of explants (zygotic embryos; ZEs) in the presence of 5 µM of 5-azaC led to the formation of a callus with EM in 10% of the cases. Transmission electron microscopy (TEM) analyzes revealed the presence of the morphological and ultrastructural features that are typical for the vacuolar type of cell death in the callus cells that were treated. A TUNEL assay confirmed the presence of DNA double-strand breaks for the callus cells that had been treated with both 5 and 50 µM 5-azaC concentrations. Analysis of the gene expression of selected cell death markers demonstrated a reduced expression of metacaspase, protein executer 1 (EX1), and thioredoxin (TRX) in the callus cells that had been treated compared to the control culture. The strongest increase in the gene activity was characteristic for glutathione S-transferase (GST). Our studies also included an analysis of the distribution of some arabinogalactan proteins (AGPs) and extensin epitopes, which can be used as markers of cells that are undergoing death in a Brachypodium distachyon tissue culture

Can ceylon leadwort (Plumbago zeylanica L.) acclimate to lead toxicity? - studies of photosynthetic apparatus efficiency

Ceylon leadwort (Plumbago zeylanica) is ornamental plant known for its pharmacological properties arising from the abundant production of various secondary metabolites. It often grows in lead polluted areas. The aim of presented study was to evaluate the survival strategy of P. zeylanica to lead toxicity via photosynthetic apparatus acclimatization. Shoots of P. zeylanica were cultivated on media with different Pb concentrations (0.0, 0.05, and 0.1 g Pb∙l−1). After a four-week culture, the efficiency of the photosynthetic apparatus of plants was evaluated by Chl a fluorescence measurement, photosynthetic pigment, and Lhcb1, PsbA, PsbO, and RuBisCo protein accumulation, antioxidant enzymes activity, and chloroplast ultrastructure observation. Plants from lower Pb concentration revealed no changes in photosynthetic pigments content and light-harvesting complex (LHCII) size, as well as no limitation on the donor side of Photosystem II Reaction Centre (PSII RC). However, the activity and content of antioxidant enzymes indicated a high risk of limitation on the acceptor side of Photosystem I. In turn, plants from 0.1 g Pb∙l−1 showed a significant decrease in pigments content, LHCII size, the amount of active PSII RC, oxygen-evolving complex activity, and significant remodeling of chloroplast ultrastructure indicated limitation of PSII RC donor side. Obtained results indicate that P. zeylanica plants acclimate to lead toxicity by Pb accumulation in roots and, depending on Pb concentration, by adjusting their photosynthetic apparatus via the activation of alternative (cyclic and pseudocyclic) electron transport pathways

Influence of soil contaminated with cadmium on cell death in the digestive epithelium of soil centipede Lithobius forficatus (Myriapoda, Chilopoda)

Cadmium is a heavy metal that is treated as an environmental pollutant (air, water, soil). In order to understand the potential effects of cadmium in soil and soil invertebrates, it is important to describe all alterations which appear at different levels in organisms. The main aim of this study was to investigate, analyze and describe the alterations caused by cadmium short- and long-term intoxication at different levels in the organisms: from tissues to cells and organelles. In addition, the activation of cell deathmechanisms that take part in homeostasismaintenance according to cadmium has been studied. Therefore, as the species for this project, a terrestrial and well-known widespread European species – the centipede Lithobius forficatus (Myriapoda, Chilopoda, Lithobiomorpha) – was chosen. This omnivorous species lives under upper layers of soil, under stones, litter, rocks, and leaves, and it is also commonly found in human habitats. The animals were divided into three groups: C – the control group, animals cultured in a horticultural soil; Cd1 – animals cultured in a horticultural soil supplemented with 80 mg/kg (dry weight) of CdCl2, 12 days – short-term exposure; Cd2 – animals cultured in a horticultural soil supplemented with 80 mg/kg (dry weight) of CdCl2, 45 days – long-term exposure. The midgut was isolated from each specimen and it was prepared for analysis using some histological, histochemical and immunohistochemical methods. Our studies showed that short-term intoxication causes intensification of autophagy and digestion of reserve material, while long-term exposure to this heavy metal causes activation of cell death processes together with inhibition of autophagy connected with the lack of reserve material. Additionally, we can infer that autophagy and cell death are nutrient deprivation-induced processes. Finally, we can conclude that short- and long-term exposure of soil centipede to cadmium affects different mechanisms and processes of cell death