59 research outputs found
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Wavelength dependent characterization of a multimode fibre endoscope
Multimode fibres have recently shown promise as miniature endoscopic probes. When used for non-linear microscopy, the bandwidth of the imaging system limits the ability to focus light from broadband pulsed lasers as well as the possibility of wavelength tuning during the imaging. We demonstrate that the bandwidth is limited by the dispersion of the off-axis hologram displayed on the SLM, which can be corrected for, and by the limited bandwidth of the fibre itself. The selection of the fibre is therefore crucial for these experiments. In addition, we show that a standard prism pulse compressor is sufficient for material dispersion compensation for multi-photon imaging with a fibre endoscope
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Time-averaged image projection through a multimode fiber
Many disciplines, ranging from lithography to opto-genetics, require high-fidelity image projection. However, not all optical systems can display all types of images with equal ease. Therefore, the image projection quality is dependent on the type of image. In some circumstances, this can lead to a catastrophic loss of intensity or image quality. For complex optical systems, it may not be known in advance which types of images pose a problem. Here we show a new method called Time-Averaged image Projection (TAP), allowing us to mitigate these limitations by taking the entire image projection system into account despite its complexity and building the desired intensity distribution up from multiple illumination patterns. Using a complex optical setup, consisting of a wavefront shaper and a multimode optical fiber illuminated by coherent light, we succeeded to suppress any speckle-related background. Further, we can display independent images at multiple distances simultaneously, and alter the effective sharpness depth through the algorithm. Our results demonstrate that TAP can significantly enhance the image projection quality in multiple ways. We anticipate that our results will greatly complement any application in which the response to light irradiation is relatively slow (one microsecond with current technology) and where high-fidelity spatial distribution of optical power is required
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Label-free CARS microscopy through a multimode fibre endoscope
Multimode fibres have recently been employed as high-resolution ultra-thin endoscopes, capable of imaging biological structures deep inside tissue in vivo. Here, we extend this technique to label-free non-linear microscopy with chemical contrast using coherent anti-Stokes Raman scattering (CARS) through a multimode fibre endoscope, which opens up new avenues for instant and in-situ diagnosis of potentially malignant tissue. We use a commercial 125 µm diameter, 0.29 NA GRIN fibre, and wavefront shaping on an SLM is used to create foci that are scanned behind the fibre facet across the sample. The chemical selectivity is demonstrated by imaging 2 µm polystyrene and 2.5 µm PMMA beads with per pixel integration time as low as 1 ms for epi-detection.Multimode fibres have recently been employed as high-resolution ultra-thin endoscopes, capable of imaging biological structures deep inside tissue in vivo. Here, we extend this technique to label-free non-linear microscopy with chemical contrast using coherent anti-Stokes Raman scattering (CARS) through a multimode fibre endoscope, which opens up new avenues for instant and in-situ diagnosis of potentially malignant tissue. We use a commercial 125 µm diameter, 0.29 NA GRIN fibre, and wavefront shaping on an SLM is used to create foci that are scanned behind the fibre facet across the sample. The chemical selectivity is demonstrated by imaging 2 µm polystyrene and 2.5 µm PMMA beads with per pixel integration time as low as 1 ms for epi-detection
Robustness of Light-Transport Processes to Bending Deformations in Graded-Index Multimode Waveguides
Light transport through a multimode optical waveguide undergoes changes when subjected to bending deformations. We show that optical waveguides with a perfectly parabolic refractive index profile are almost immune to bending, conserving the structure of propagation-invariant modes. Moreover, we show that changes to the transmission matrix of parabolic-index fibers due to bending can be expressed with only two free parameters, regardless of how complex a particular deformation is. We provide detailed analysis of experimentally measured transmission matrices of a commercially available graded-index fiber as well as a gradient-index rod lens featuring a very faithful parabolic refractive index profile. Although parabolic-index fibers with a sufficiently precise refractive index profile are not within our reach, we show that imaging performance with standard commercially available graded-index fibers is significantly less influenced by bending deformations than step-index types under the same conditions. Our work thus predicts that the availability of ultraprecise parabolic-index fibers will make endoscopic applications with flexible probes feasible and free from extremely elaborate computational challenges
Observing distant objects with a multimode fibre-based holographic endoscope
Holographic wavefront manipulation enables converting hair-thin multimode
optical fibres into minimally invasive lensless imaging instruments conveying
much higher information densities than conventional endoscopes. Their most
prominent applications focus on accessing delicate environments, including deep
brain compartments, and recording micrometre-scale resolution images of
structures in close proximity to the distal end of the instrument. Here, we
introduce an alternative 'farfield' endoscope, capable of imaging macroscopic
objects across a large depth of field. The endoscope shaft with dimensions of
0.20.4 mm consists of two parallel optical fibres, one for
illumination and the second for signal collection. The system is optimized for
speed, power efficiency and signal quality, taking into account specific
features of light transport through step-index multimode fibres. The
characteristics of imaging quality are studied at distances between 20 and 400
mm. As a proof-of-concept, we provide imaging inside the cavities of a sweet
pepper commonly used as a phantom for biomedically relevant conditions.
Further, we test the performance on a functioning mechanical clock, thus
verifying its applicability in dynamically changing environments. With
performance reaching the standard definition of video endoscopes, this work
paves the way towards the exploitation of minimally-invasive holographic
micro-endoscopes in clinical and diagnostics applications.Comment: 9+6 pages, 4+5 figure
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Observing distant objects with a multimode fiber-based holographic endoscope
Holographic wavefront manipulation enables converting hair-thin multimode optical fibers into minimally invasive lensless imaging instruments conveying much higher information densities than conventional endoscopes. Their most prominent applications focus on accessing delicate environments, including deep brain compartments, and recording micrometer-scale resolution images of structures in close proximity to the distal end of the instrument. Here, we introduce an alternative "far-field"endoscope capable of imaging macroscopic objects across a large depth of field. The endoscope shaft with dimensions of 0.2 × 0.4 mm2 consists of two parallel optical fibers: one for illumination and the other for signal collection. The system is optimized for speed, power efficiency, and signal quality, taking into account specific features of light transport through step-index multimode fibers. The characteristics of imaging quality are studied at distances between 20 mm and 400 mm. As a proof-of-concept, we provide imaging inside the cavities of a sweet pepper commonly used as a phantom for biomedically relevant conditions. Furthermore, we test the performance on a functioning mechanical clock, thus verifying its applicability in dynamically changing environments. With the performance reaching the standard definition of video endoscopes, this work paves the way toward the exploitation of minimally invasive holographic micro-endoscopes in clinical and diagnostics applications. © 2021 Author(s)
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Robustness of Light-Transport Processes to Bending Deformations in Graded-Index Multimode Waveguides
Light transport through a multimode optical waveguide undergoes changes when subjected to bending deformations. We show that optical waveguides with a perfectly parabolic refractive index profile are almost immune to bending, conserving the structure of propagation-invariant modes. Moreover, we show that changes to the transmission matrix of parabolic-index fibers due to bending can be expressed with only two free parameters, regardless of how complex a particular deformation is. We provide detailed analysis of experimentally measured transmission matrices of a commercially available graded-index fiber as well as a gradient-index rod lens featuring a very faithful parabolic refractive index profile. Although parabolic-index fibers with a sufficiently precise refractive index profile are not within our reach, we show that imaging performance with standard commercially available graded-index fibers is significantly less influenced by bending deformations than step-index types under the same conditions. Our work thus predicts that the availability of ultraprecise parabolic-index fibers will make endoscopic applications with flexible probes feasible and free from extremely elaborate computational challenges
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Polarization-resolved second-harmonic generation imaging through a multimode fiber
Multimode fiber-based endoscopes have recently emerged as a tool for minimally invasive endoscopy in tissue, at depths well beyond the reach of multiphoton imaging. Here, we demonstrate label-free second-harmonic generation (SHG) microscopy through such a fiber endoscope. We simultaneously fully control the excitation polarization state and the spatial distribution of the light at the fiber tip, and we use this to implement polarization-resolved SHG imaging, which allows imaging and identification of structural proteins such as collagen and myosin. We image mouse tail tendon and heart tissue, employing the endoscope at depths up to 1 mm, demonstrating that we can differentiate these structural proteins. This method has the potential for enabling instant and in situ diagnosis of tumors and fibrotic conditions in sensitive tissue with minimal damage
Optical sorting and detection of sub-micron objects in a motional standing wave
An extended interference pattern close to surface may result in both a
transmissive or evanescent surface fields for large area manipulation of
trapped particles. The affinity of differing particle sizes to a moving
standing wave light pattern allows us to hold and deliver them in a
bi-directional manner and importantly demonstrate experimentally particle
sorting in the sub-micron region. This is performed without the need of fluid
flow (static sorting). Theoretical calculations experimentally confirm that
certain sizes of colloidal particles thermally hop more easily between
neighboring traps. A new generic method is also presented for particle position
detection in an extended periodic light pattern and applied to characterization
of optical traps and particle behaviorComment: 5 pages, 6 figures, Optical Trapping pape
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Comparison of nematic liquid-crystal and DMD based spatial light modulation in complex photonics
Digital micro-mirror devices (DMDs) have recently emerged as practical spatial light modulators (SLMs) for applications in photonics, primarily due to their modulation rates, which exceed by several orders of magnitude those of the already well-established nematic liquid crystal (LC)-based SLMs. This, however, comes at the expense of limited modulation depth and diffraction efficiency. Here we compare the beam-shaping fidelity of both technologies when applied to light control in complex environments, including an aberrated optical system, a highly scattering layer and a multimode optical fibre. We show that, despite their binary amplitude-only modulation, DMDs are capable of higher beam-shaping fidelity compared to LC-SLMs in all considered regimes
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