Multiplication of lavender (L. angustifolia) and lavandin (Lavandula x intermedia) in explant culture

Lavender plants are preferably propagated vegetatively. The aim of the work was to propose the composition of nutrient media for in vitro multiplication of plants of Lavandula angustifolia (var. Krajová and Beta) and Lavandula x intermedia (var. Budrovka and Grosso). Explant cultures were initiated from apical or nodal segments. Apical segments were more suitable and regenerated the highest number of shoots in MS medium supplemented with 1 mg/L BAP with or without 0.1 mg/L IAA in all used varieties. Nodal segments are suitable for var. Budrovka, where the number of shoots was not statistically different between the apical or nodal segments and among the media tested. Shoot multiplication of L. angustifolia varieties was the most effective in the MS media with 0.5 or 1 mg/L BAP. For variety Grosso, the optimal medium was MS with 1 mg/L BAP, while var. Budrovka regenerated a similar number of shoots in all media supplemented with 0.5 or 1 mg/L BAP with or without 0.1 mg/L IAA. All genotypes rooted with the highest frequency on growth regulator-free medium with a half dose of MS salts. After transplanting into the soil and ex vitro acclimatization, plantlets survivability after 10 weeks was 78.9 – 87.7%, except for var. Beta with a lower survivability of 50%. The proposed procedures enable a rapid plant multiplication of varieties Budrovka, Grosso and Krajová. With the Beta variety, it is advisable to continue working on the optimization of nutrient media to verify whether the procedures can be further optimized

Razlike pri nastajanju polifenolov v delih rastlin in fazah rasti pri izbranih kultivarjih ajde

Dynamics of polyphenolics formation in different plant parts and different growth phases of selected buckwheat cultivar

PREPARATION OF DECADIENAL AND DECATRIENAL THROUGH BIOTRANSFORMATION

Many aroma active compounds can be produced by means of plant enzyme-catalysed reactions. The ability of the eggplant fruit enzymes to catalyse biotransformation of distilled sunflower and linseed oils to two important flavour compounds: (2E,4E)-decadienal, and (2E,4E,7Z)-decatrienal was investigated under various conditions. The results show that maximum yields were reached after 20 minutes of reaction for both compounds (2E,4E)-decadienal (1.24 g/kg of egg fruit) and (2E,4E,7Z)-decatrienal (1.4 g/kg of egg fruit), respectively. The extraction efficiency of ethyl acetate was higher compared to the use of isoamyl alcohol for both compounds

Concentrations of phenolic acids are differently genetically determined in leaves, flowers, and grain of common buckwheat (Fagopyrum esculentum Moench)

Common buckwheat (Fagopyrum esculentum Moench) is a valuable source of proteins, B vitamins, manganese, tryptophan, phytochemicals with an antioxidant effect, and the natural flavonoid rutin. Due to its composition, buckwheat supports the human immune system, regulates blood cholesterol, and is suitable for patients with diabetes or celiac disease. The study aimed to compare the allocation of selected phenolic acids (neochlorogenic acid, chlorogenic acid, transcaffeic acid, trans-p-coumaric acid, trans-sinapic acid, trans-ferulic acid) and flavonoids (rutin, vitexin, quercetin, kaempferol) in the leaves, flowers, and grain of buckwheat cultivars of different origin. The content of individual phenolics was determined by the HPLC-DAD method. The results confirmed the determining role of cultivar on the relative content of chlorogenic acid, trans-caffeic acid, transsinapic acid, vitexin, and kaempferol in buckwheat plants. A significantly negative correlation among concentrations of phenolic acids in different common buckwheat plant parts shows that there are different mechanisms of genetic influences on the concentration of phenolic substances in common buckwheat flowers, leaves, and grain. These differences should be taken into account when breeding buckwheat for a high concentration of selected phenolic substances

Antimicrobial and Antioxidant Properties of Four Lycopus Taxa and an Interaction Study of Their Major Compounds

The compositions of leaf infusions of three genotypes of Lycopus europaeus L. with origins in central Europe, namely L. europaeus A (LeuA), L. europaeus B (LeuB), and L. europaeus C (LeuC), and one genotype of L. exaltatus (Lex), were examined by LC-MS-DAD (Liquid Chromatography Mass Spectrometry and Diode Array Detection) analysis. This revealed the presence of thirteen compounds belonging to the groups of phenolic acids and flavonoids, with a predominance of rosmarinic acid (RA) and luteolin-7-O-glucuronide (LGlr). The antimicrobial activity of leaf infusions was tested on the collection strains of Gram-positive and Gram-negative bacteria, and on the clinical Staphylococcus aureus strains. We detected higher activity against Gram-positive bacteria, of which the most susceptible strains were those of Staphylococcus aureus, including methicillin-resistant and poly-resistant strains. Furthermore, we examined the antioxidant activity of leaf infusions using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) methods, and on NIH/3T3 cell lines using dichlorodihydrofluorescein diacetate (DCFH-DA). We also studied the mutual interactions between selected infusions, namely RA and/or LGlr. In the mixtures of leaf infusion and RA or LGlr, we observed slight synergism and a high dose reduction index in most cases. This leads to the beneficial dose reduction at a given antioxidant effect level in mixtures compared to the doses of the parts used alone. Therefore, our study draws attention to further applications of the Lycopus leaves as a valuable alternative source of natural antioxidants and as a promising topical antibacterial agent for medicinal use

Structural changes in agriculture since EU accession in Slovakia, the Czech Republic and Hungary

The collaboration that has led to the publication of this book can be traced back to April 2013, when the Research Institute of Agricultural and Food Economics (VÚEPP) in Bratislava, Slovak Republic, approached the Research Institute of Agricultural Economics (AKI) in Budapest, Hungary, with a view to establishing a programme of bilateral cooperation. The approach was very positively received by AKI, and the idea rapidly developed into a plan for a series of trilateral cooperation activities that also included the Institute of Agricultural Economics and Information (IAEI) in Praha, Czech Republic. Representatives of the three institutes met in Budapest at the end of May, 2013 and agreed to work together to enhance mutual research collaboration in the field of agricultural economics, share information and discuss issues related to agricultural economics in the three countries, and establish a coordination group composed by members of the three research institutes. The cooperation was formalised through the signing, in December 2013, of a trilateral Agreement covering the period 2014-2016 covering the following topics: (a) publication of individual or common papers in the institutes’ journals or other journals, and exchange of journals between institutes; (b) exchange of experience via trilateral meetings of specialists; (c) cooperation with other scientific entities and support for affiliation to international networks or construction of a specific network in the institutes’ common field of research interest; (d) applications and participation in common international projects; and (e) participation at international meetings with common research/papers and cooperation in organisation of different international meetings. The three institutes agreed that this would be an excellent way to better disseminate, nationally and internationally, the results of their research work and to open new perspectives to future mutual cooperation. The centrepiece of the programme of cooperation was a trilateral research project entitled “The CAP Impact on the Effectiveness of Use of Agricultural Production Factors and the Economic Efficiency of Agricultural Production and Product Sectors in the Slovak Republic, the Czech Republic and Hungary”. The general objective of this project has been: research on rural areas and the agrifood sector by sectoral analysis, country comparisons, identification of positive and negative influences on the rural and agricultural economy, dissemination of research results, and proposals for future policies in the field. Initially intended to cover four topics (implementation of the new Common Agricultural Policy (CAP), agricultural land ownership and related issues, competitiveness and profitability of crop and animal production, and the efficiency of food industry production), further discussion led to eight topics of joint research being agreed