Ümmünolojik gebelik testi üretimi.

TEZ227Tez (Yüksek Lisans) -- Çukurova Üniversitesi, Adana, 1985.Kaynakça (s. 38-40) var.40 s. : res. ; 28 cm.

Cytotoxic effect of n-acetyl cysteine in DU145 human prostate cancer cells

Prostate cancer is one of the most common types of cancer in men all over the world. Chemotherapy, radiotherapy and surgery are conventional cancer treatment modalities. However, these modalities have many complications and side effects. Therefore, it is very important to support the treatment with compounds that will reduce the side effects and increase the effectiveness of the treatment. The aim of this study is to investigate the cytotoxic effects of N-acetyl cysteine (NAC), an antioxidant, on human prostate cancer cells. This study was performed on DU145 human prostate cancer cells. The cells were divided into 7 groups (control and 0.2, 0.5, 1, 2, 5 and 10 mM NAC groups). Cell index was determined using real time monitoring of electrical impedance in all groups. Apoptotic activity was determined using flow cytometry. Apoptotic activity was measured only 5 and 10 mM NAC groups. The cell index increased significantly at lower doses of NAC (0.2, 0.5, 1, 2 mM) but decreased at higher doses (5 and 10 mM). NAC significantly increased percentage of early and late apoptotic cells at 10 mM concentration. Percentage of necrotic cell significantly increased at 5 and 10 mM concentrations. Our data showed that NAC could significantly inhibit the proliferation of DU145 human prostate cancer cells in high doses. In addition, in this study for the first time, we report that NAC enhances apoptotic activity of human prostate DU145 cancer cells. These findings denote the antitumor properties of NAC. © 2019 Marmara University Press.This study was supported by TUBITAK (Turkish Scientific and Technical Research Institute) with 1919B011603685 grant number

Cochlear electrophysiology and histopathology of rats exposed to intratympanic silica nanoparticles

WOS:000497661000015Toxic effects of silica nanoparticle of rats exposed intratympanically for 7 days were investigated using brainstem auditory evoked potential and distortion product otoacoustic emission techniques. The histopathological changes were evaluated with light microscopy and field emission-scanning electron microscopy were demonstrated in cochleas using based energy-dispersive X-ray spectroscopy. The reduction in the amplitude and prolongation at the peak latency and interpeak latencies of brainstem auditory evoked potential were observed. Silica nanoparticles significantly reduced distortion product otoacoustic emission amplitude in the 2, 3 and 4 kHz frequencies. In the light microscopic evaluation, the samples obtained from the experimental group showed a significant loss of hair cells. Field emission scanning electron microscope analysis of experimental group showed considerable hair cell damage compared to control group. The findings of this study show that intratympanic administration of silica nanoparticles may lead to hearing impairment by causing structural changes in cochlear hair cells.Research Fund of Mersin University in TurkeyMersin University [BAP- 2017-1-TP2-2143]This study was supported by the Research Fund of Mersin University in Turkey with Project Number: BAP- 2017-1-TP2-2143

Protective effect of Funalia trogii crude extract on deltamethrin-induced oxidative stress in rats

WOS: 000284818400031In this study the protective effects of cold buffer extract of Funalia trogii ATCC 200800 (FtE) and vitamin E (VitE) on oxidative stress induced with deltamethrin using oral administration in rats were investigated. Deltamethrin treatment caused an increase in liver enzyme activities of aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) (p < 0.05); however, it caused a decrease in activities of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione reductase (GRd) when compared to control group (p < 0.05). Activities of AST, ALT, ALP enzymes and level of thiobarbituric acid reactive substances (TBARS) decreased significantly after VitE administration (p < 0.05). Both enzyme activities and TBARS levels were found similar in VitE and FtE treated rats shortly after pesticide administration (p < 0.05). In conclusion, it appears that FtE prepared in cold buffer has capability to prevent the liver damage like VitE against the toxic effect of deltamethrin. (C) 2010 Elsevier Ltd. All rights reserved

The effect of 4.5 G (LTE Advanced-Pro network) mobile phone radiation on the optic nerve

WOS:000670115600001 PubMed ID:33653184Purpose Rapid development in mobile phone technologies increase the average mobile phone usage duration. This increase also triggers exposure to radiofrequency radiation (RF), which is a risk factor for the health. In this study, it was aimed to investigate the effect of mobile phone working with LTE-Advanced Pro (4.5 G) mobile network on the optic nerve, which is responsible for the transmission of visual information. Material and methods Thirty-two rats divided into two groups as control (no RF, sham exposure) and experimental (RF exposure using a mobile phone with LTE-Advanced Pro network; 2 hours/day, 6 weeks). The visual evoked potential (VEP) was recorded and determined amplitudes and latencies of VEP waves. Optic nerve malondialdehyde level, catalase and superoxide dismutase activities were determined. Furthermore, ultrastructural and morphometric changes of optic nerve were evaluated. Results In VEP recordings, the mean VEP amplitudes of experimental group were significantly lower than control group. In ultrastructural evaluation, myelinated nerve fibres and glial cells were observed in normal histologic appearance both in sham and experimental group. However, by performing morphometric analysis, in the experimental group, axonal diameter and myelin thickness were shown to be lower and the G-ratio was higher than in the sham group. In the experimental group, malondialdehyde level was significantly higher and superoxide dismutase and catalase activities were significantly lower than sham group. There was a high correlation between VEP wave amplitudes and oxidative stress markers. Conclusion Findings obtained in this study support optic nerve damage. These results point out an important risk that may decrease the quality of life