A geodiversidade como elemento dos rios patrimônio: o exemplo do São Francisco, Rio da Unidade Nacional – da nascente até a cachoeira Casca d’Anta

Os rios são partes integrantes da geodiversidade e têm proximidade especial com a sociedade. O patrimônio fluvial é caracterizado por rios incomuns com aspectos singulares de geodiversidade, valores culturais e históricos e elementos ecológicos e paisagísticos de destacada qualidade cênica. Nesse contexto, o presente estudo visou identificar o conjunto de elementos ou lugares de interesse que poderiam ser utilizados para reconhecer o patrimônio fluvial baseado em valores de geodiversidade ao longo de um trecho de 17 km entre a cabeceira do Rio São Francisco e a Cachoeira da Casca d’Anta no Parque Nacional da Serra da Canastra (estado de Minas Gerais, Brasil). Aqui, sete elementos do patrimônio fluvial foram reconhecidos: i) Conjunto paisagístico da Serra da Canastra; ii) nascente histórica do rio São Francisco; iii) Curral de Pedras; iv) Cachoeira da Casca d’Anta; v) área de nidificação do Pato Mergulhão Mergus octosetaceus; vi) extração de diamantes; vii) o Queijo Canastra. Os elementos do patrimônio fluvial identificados ressaltam a importância ecológica, cultural, histórica e turística/econômica do trecho fluvial analisado. Além disso, tais elementos demonstram a necessidade de melhorias nas legislações existentes e instrumentos mais efetivos para a conservação e gestão dos rios e sua conservação.Rivers are key parts of geodiversity and have special proximity with society. Hence, river heritage is characterized by uncommon rivers with singular aspects of geodiversity, historical and cultural values, ecological and landscape elements of high scenic quality. In such context, the present study aimed to identify the set of elements or places of interest which could be used to recognize river heritage based on geodiversity values along a 17 km stretch between São Francisco river headwaters and the Casca d’Anta waterfall within Canastra Range National Park (Minas Gerais State - Brazil). Here, seven elements of river heritage were recognized: i) landscape set of the Canastra Range; ii) São Francisco historic headwater; iii) Curral de Pedras; Casca d’Anta waterfall; v) Nesting area of the Mergus octosetaceus; vi) diamond mining; vii) the Canastra cheese. The identified elements of river heritage highlight the scientific, ecological, cultural, historic and touristic/economic importance of the analyzed. stretch Moreover, such elements illustrate need for improvement of the existing legislations and more effective instruments of river management and river conservation.The authors would like to thank FAPEMIG for the doctoral scholarship granted to Carmélia Oliveira and CNPQ for the Level 2 Research Productivity Grant for Úrsula Ruchkys and Paulo de Tarso Amorim Castro

The use of body growth and kinship data from 16 generations for predicting Thoroughbred performance

Thoroughbred horses have been intensely raised for their athletic potential that is correlated with morphological parameters (i.e., body weight - BW, and withers height - WH). Optimum and consistent body development is aimed, but excessive growth rates may lead to the development of orthopedic diseases. This study aimed to generate growth rate curves and prediction models of Thoroughbred horses by analyzing BW and WH data collected monthly over 16 years of 378 animals (23.6 animals/year). The animals were checked from birth to 18 months (160 colts and 181 fillies) on a farm in the south of Brazil. A prediction performance ARIMA model was developed based on the BW and WH of the foals using a maximum and minimum range of 7320 observations. BW and WH were 54,1 kg and 102,5 cm at birth and 397,8 kg and 150,6 cm at 18 months of age, respectively. No differences were found between sex at any age. Moreover, we have established a nonlinear function for the growth curve and on average, foals were expected to get 7.4 times heavier and 1.5 times taller when animals were fully grown. Males showed greater BW uniformity than females, as females had a lower minimum BW than males. Significant statistical differences (P < 0,05%) were reported for BW and WH of foals between pairs of sires highlighting the kinship (paternal) effect on the animals’ development. Seventy-two pairs of stallions showed statistical relevance for BW and 91 for WH. The ARIMA model produced a linear trend of BW and WH for the forecasted years. In conclusion, we recommend that careful sire selection and adequate health (i.e., parasite control, vaccination), and nutrition strategies must be adopted to achieve superior body growth as estimated by the predicting model (positive scenario). The present protocol shall be used in studs worldwide to monitor horse development. The spreadsheet is available on request to the corresponding authors

Bottom-Up Proteomic Analysis of Polypeptide Venom Components of the Giant Ant Dinoponera Quadriceps

Ant species have specialized venom systems developed to sting and inoculate a biological cocktail of organic compounds, including peptide and polypeptide toxins, for the purpose of predation and defense. The genus Dinoponera comprises predatory giant ants that inoculate venom capable of causing long-lasting local pain, involuntary shaking, lymphadenopathy, and cardiac arrhythmias, among other symptoms. To deepen our knowledge about venom composition with regard to protein toxins and their roles in the chemical&ndash;ecological relationship and human health, we performed a bottom-up proteomics analysis of the crude venom of the giant ant D. quadriceps, popularly known as the &ldquo;false&rdquo; tocandiras. For this purpose, we used two different analytical approaches: (i) gel-based proteomics approach, wherein the crude venom was resolved by denaturing sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and all protein bands were excised for analysis; (ii) solution-based proteomics approach, wherein the crude venom protein components were directly fragmented into tryptic peptides in solution for analysis. The proteomic data that resulted from these two methodologies were compared against a previously annotated transcriptomic database of D. quadriceps, and subsequently, a homology search was performed for all identified transcript products. The gel-based proteomics approach unequivocally identified nine toxins of high molecular mass in the venom, as for example, enzymes [hyaluronidase, phospholipase A1, dipeptidyl peptidase and glucose dehydrogenase/flavin adenine dinucleotide (FAD) quinone] and diverse venom allergens (homologous of the red fire ant Selenopsis invicta) and venom-related proteins (major royal jelly-like). Moreover, the solution-based proteomics revealed and confirmed the presence of several hydrolases, oxidoreductases, proteases, Kunitz-like polypeptides, and the less abundant inhibitor cysteine knot (ICK)-like (knottin) neurotoxins and insect defensin. Our results showed that the major components of the D. quadriceps venom are toxins that are highly likely to damage cell membranes and tissue, to cause neurotoxicity, and to induce allergic reactions, thus, expanding the knowledge about D. quadriceps venom composition and its potential biological effects on prey and victims

Insights into the Hypertensive Effects of Tityus serrulatus Scorpion Venom: Purification of an Angiotensin-Converting Enzyme-Like Peptidase

The number of cases of envenomation by scorpions has grown significantly in Brazil since 2007, with the most severe cases being caused by the Tityus serrulatus scorpion. Although envenomed patients mostly suffer neurotoxic manifestations, other symptoms, such as hypertension, cannot be exclusively attributed to neurotoxins. Omics analyses have detected plentiful amounts of metalloproteases in T. serrulatus venom. However, the roles played by these enzymes in envenomation are still unclear. Endeavoring to investigate the functions of scorpion venom proteases, we describe here for the first time an Angiotensin I-Converting Enzyme-like peptidase (ACE-like) purified from T. serrulatus venom. The crude venom cleaved natural and fluorescent substrates and these activities were inhibited by captopril. Regarding the serum neutralization, the scorpion antivenom was more effective at blocking the ACE-like activity than arachnid antivenom, although neither completely inhibited the venom cleavage action, even at higher doses. ACE-like was purified from the venom after three chromatographic steps and its identity was confirmed by mass spectrometric and transcriptomic analyses. Bioinformatics analysis showed homology between the ACE-like transcript sequences from Tityus spp. and human testis ACE. These findings advance our understanding of T. serrulatus venom components and may improve treatment of envenomation victims, as ACE-like may contribute to envenomation symptoms, especially the resulting hypertension

An integrated analysis of mRNA and sRNA transcriptional profiles in Coffea arabica L. roots: insights on nitrogen starvation responses

International audienceCoffea arabica L. is an important agricultural commodity, accounting for 60% of traded coffee worldwide. Nitrogen (N) is a macronutrient that is usually limiting to plant yield; however, molecular mechanisms of plant acclimation to N limitation remain largely unknown in tropical woody crops. In this study, we investigated the transcriptome of coffee roots under N starvation, analyzing poly-A+ libraries and small RNAs. We also evaluated the concentration of selected amino acids and N-source preferences in roots. Ammonium was preferentially taken up over nitrate, and asparagine and glutamate were the most abundant amino acids observed in coffee roots. We obtained 34,654 assembled contigs by mRNA sequencing, and validated the transcriptional profile of 12 genes by RT-qPCR. Illumina small RNA sequencing yielded 8,524,332 non-redundant reads, resulting in the identification of 86 microRNA families targeting 253 genes. The transcriptional pattern of eight miRNA families was also validated. To our knowledge, this is the first catalog of differentially regulated amino acids, N sources, mRNAs, and sRNAs in Arabica coffee roots