Rapid Determination Of Orotic Acid Level in Urine By Capillary Electrophoresis Coupled with Contactless Conductivity Detection

İdrarda orotik asidin aşırı atılımı üre döngüsü bozukluğu ve pirimidin sentez bozukluğu gibi doğumsal metabolik hastalıklarda görülür. Bu nedenle orotik asidin hızlı bir şekilde tayini hayati öneme sahiptir. Bu çalışmada idrar örneklerinde orotik asidin kantitatif düzeylerinin belirlenmesi için yeni, basit, ucuz ve hızlı bir kapiler elektroforetik analiz yöntemi geliştirildi. Orotik asidin temassız iletkenlik dedektörü (C4D) kullanılarak kapiler elektroforez yöntemi ile ayrımı için pH’ı 6,5 olan 20 mM 4-Morfolinetansülfonik asit (MES), 10 mM Histidin ve 0,1 mM setil trimetilamonyum bromür (CTAB) içeren bir ça-lışma tamponu ile geliştirildi. Orotik asidin ayrılması yaklaşık 3,5 dakika gibi kısa bir sürede tamamlandı. Göç zamanlarının ve düzeltilmiş pik alanlarının tekrarlanabilirliği sırasıyla %RSD olarak 1,25 ve 4,31 olarak bulundu. En düşük dedeksiyon limiti (LOD) 0,733 mg / L ve en düşük tayin limiti de (LOQ) 2,443 mg / L olarak bulundu. Metodun idrarda orotik asit düzeylerinin tayin edilmesinde kolaylıkla ve hassasiyetle kullanılabileceği gösterildi.Excessive excretion of orotic acid in urine arises as a result of some inborn metabolic diseases such as urea cycle disorders and pyrimidine synthesis disorders. Therefore, rapid determination of orotic acid in the neonatal period is vital. Existing methods for measuring orotic acid levels in urine are both time consuming and expensive. Consequently, rapid definition of the orotic acid level in human urine is important. In this current study a novel, simple, cost effective and rapid (fast) capillary electrophoretic analysis method was developed for quantitative measurement of orotic acid content in urine samples. Determination of orotic acid by employing capillary electrophoresis coupled with contactless conductivity detection was achieved by using, 20 mM 4-Morpholineethanesulfonic acid (MES), 10 mM Histidine, 0.1 mM Cetyltrimethylammonium Bromide (CTAB) at pH of 6.5 as optimum electrolyte solution. Orotic acid was well separated from the sample constituents within a short analysis time less than 3.5 minutes. The precisions for the migration times and corrected peak areas were calculated as 1.25 and 4.31 RSD% respectively. Limit of detection and limit of quantification of the proposed method were also calculated as 0.733 mg / L and 2.443 mg / L respectively. The developed sensitive and facile capillary electrophoresis method was validated and applicability of the method was successfully demonstrated by assessment of orotic acid content in urine

Application of preventive maintenance planning in a parquet enterprise

Sevim Korkut, Derya/0000-0002-0963-016X; Sevim Korkut, Derya/0000-0001-7903-1843WOS: 000266174000042In this study, the process of a parquet enterprise's transition to preventive maintenance which has been implementing maintenance technique in case of breakdown was investigated. During that transition process, follow up forms for maintenance-repair, breakdown and spare parts were prepared and the implementation processes were followed. At the end of the implementation, it was identified that breakdowns have declined, anxiety and turmoil brought about by breakdowns have been prevented, the life span of the machines soared, labour-borne accidents declined and production quality increased

The effects of different degrees of head-of-bed elevation on the respiratory pattern and drainage following thyroidectomy: A randomized controlled trial

Background: The patient's position is important for ensuring patient comfort and preventing complications after thyroidectomy.Objectives: This study was carried out to determine the effects of different degrees of head-of-bed elevation (HOBE) on the respiratory pattern and drainage following thyroidectomy and to provide suggestions for evidence-based clinical practice.Methods: The sample of this prospective, parallel arm, randomized controlled trial included 114 patients undergoing thyroidectomy in a university hospital in Turkey. The patients were randomly assigned (1:1:1) to supine 0 degrees (baseline), 30 degrees and 45 degrees HOBE groups. Respiratory pattern including respiratory rate (RR), peripheral oxygen saturation (SpO(2)) and dyspnea, and drainage including amount of drainage and hematoma formation were evaluated at the 1st, 2nd, 3rd and 4th hours following thyroidectomy.Results: The majority of the patients (83.3%) were female and 84.2% had undergone total thyroidectomy. The mean RR (18.47, 95% CI=17.85-19.09) of the patients in the supine 30 degrees HOBE group at the 1st hour was significantly higher than that of the patients in the supine 0 degrees group (17.32, 95% CI=16.88-17.76; p0.05). The amount of drainage was significantly higher in the supine 0 degrees group at the 2nd hour than that of the patients in the supine 45 degrees HOBE group (5.92 +/- 5.18; 3.34 +/- 5.56 respectively; p <0.05). None of the patients in the groups had hematoma formation. While no patient in the supine 30 degrees HOBE group had dyspnea, dyspnea occurred in 9 patients in the supine 0 degrees group and in 3 patients in the supine 45 degrees HOBE group.Conclusion: This study showed that different HOBE positions resulted in clinically insignificant changes on the RR and amount of drainage during the first 4 hours following thyroidectomy but did not affect SpO(2) value

Automatic diagnosis of cardiovascular disorders by sub images of the ECG signal using multi-feature extraction methods and randomized neural network

Electrocardiography has been employed successfully in medicine for many years to provide vital knowledge about the cardiovascular system. Although processing and evaluation of electrocardiogram (ECG) signals provide helpful information in the detection of anomalies in the vessel, diagnosis of heart defect, and treatment of diseases, multi-channel ECG signals have been started to be employed in order to achieve higher success. Utilizing a multi-channel ECG signal instead of a one-channel ECG signal yields more adequate achievements but require higher complexity in analysis and higher computational cost. To achieve faster and accurate results in multichannel ECG signals, an artificial intelligence-based automatic diagnosis system employing the texture features of two-dimensional images, which are constructed by projecting the ECG signal vector as a row of the image, is proposed. The hypothesis proposed in this study conjectures that these texture features in the images contain determinative indicators of various diseases (cardiovascular abnormalities/disturbance) even for the short-time intervals. Accordingly, the main contribution of this study is to expose that detection of cardiovascular defects can be done with the classical image texture methods by utilizing multi-channel biomedical signals in a sufficiently short-time-interval. The methodology has been implemented in different time intervals of a large dataset constructed from a diverse population that is labeled as one normal sinus rhythm type and eight abnormal types of ECG signals. The accuracy of this hypothesis has been proven by achieving high detection rates of identifying cardiac abnormalities and reduced the computational load of the processing system without any sacrificing accuracy

Capillary electrophoresis with capacitively coupled contactless conductivity detection for the determination of urinary ethylmalonic acid for the diagnosis of ethylmalonic aciduria

Ethylmalonic acid is a metabolic organic acid, and its accumulation in urine is diagnostic of ethylmalonic aciduria. In this study, a simple and fast method employing capillary electrophoresis equipped with capacitively coupled contactless conductivity detection was developed for the detection of ethylmalonic acid in urine samples. The optimized electrophoretic separation was performed in 50 mmol/L 2-(N-morpholino)ethanesulfonic acid solution, buffered at a pH of 6.5, and contained 0.13 mmol/L cetyltrimethylammonium bromide as an electroosmotic modifier. Electrophoresis was run at 28 kV in reversed polarity. The linear range of ethylmalonic acid concentration was between 1 and 100 mg/L with a regression coefficient of 0.9998. This method had good intra- and interday precision with <5% relative standard deviations. The detection limit (signal-to-noise ratio = 3) and the quantification limit (signal-to-noise ratio = 10) values were 0.139 and 0.466 mg/L, respectively. Using our optimized conditions, the method was successfully employed for the detection of ethylmalonic acid in urine sample of ethylmalonic aciduria patient.Research Foundation of Istanbul Technical UniversityIstanbul University [TDK-2018-41370]Research Foundation of Istanbul Technical University, Grant/Award Number: TDK-2018-4137

Capillary electrophoresis with capacitively coupled contactless conductivity detection for the determination of urinary ethylmalonic acid for the diagnosis of ethylmalonic aciduria

Ethylmalonic acid is a metabolic organic acid, and its accumulation in urine is diagnostic of ethylmalonic aciduria. In this study, a simple and fast method employing capillary electrophoresis equipped with capacitively coupled contactless conductivity detection was developed for the detection of ethylmalonic acid in urine samples. The optimized electrophoretic separation was performed in 50 mmol/L 2-(N-morpholino)ethanesulfonic acid solution, buffered at a pH of 6.5, and contained 0.13 mmol/L cetyltrimethylammonium bromide as an electroosmotic modifier. Electrophoresis was run at 28 kV in reversed polarity. The linear range of ethylmalonic acid concentration was between 1 and 100 mg/L with a regression coefficient of 0.9998. This method had good intra- and interday precision with <5% relative standard deviations. The detection limit (signal-to-noise ratio = 3) and the quantification limit (signal-to-noise ratio = 10) values were 0.139 and 0.466 mg/L, respectively. Using our optimized conditions, the method was successfully employed for the detection of ethylmalonic acid in urine sample of ethylmalonic aciduria patient.Research Foundation of Istanbul Technical UniversityIstanbul University [TDK-2018-41370]Research Foundation of Istanbul Technical University, Grant/Award Number: TDK-2018-4137

Capillary electrophoresis with capacitively coupled contactless conductivity detection for the determination of urinary ethylmalonic acid for the diagnosis of ethylmalonic aciduria

Ethylmalonic acid is a metabolic organic acid, and its accumulation in urine is diagnostic of ethylmalonic aciduria. In this study, a simple and fast method employing capillary electrophoresis equipped with capacitively coupled contactless conductivity detection was developed for the detection of ethylmalonic acid in urine samples. The optimized electrophoretic separation was performed in 50 mmol/L 2-(N-morpholino)ethanesulfonic acid solution, buffered at a pH of 6.5, and contained 0.13 mmol/L cetyltrimethylammonium bromide as an electroosmotic modifier. Electrophoresis was run at 28 kV in reversed polarity. The linear range of ethylmalonic acid concentration was between 1 and 100 mg/L with a regression coefficient of 0.9998. This method had good intra- and interday precision with <5% relative standard deviations. The detection limit (signal-to-noise ratio = 3) and the quantification limit (signal-to-noise ratio = 10) values were 0.139 and 0.466 mg/L, respectively. Using our optimized conditions, the method was successfully employed for the detection of ethylmalonic acid in urine sample of ethylmalonic aciduria patient

The effect of bed head slope on patients’ outcomes in early postoperative period after thyroidectomy : A randomized controlled trial.

THE EFFECT OF BED HEAD SLOPE ON PATIENTS&rsquo; OUTCOMES IN EARLY POSTOPERATIVE PERIOD AFTER THYROIDECTOMY: A RANDOMIZED CONTROLLED TRIAL Serpil Y&uuml;ksel* , Seher Deniz &Ouml;ztekin, Zeynep Temiz, G&uuml;lay Altun Uğraş, Emel Şeng&uuml;l, Serkan Teks&ouml;z, Nihal Sunal, İlhan &Ouml;ztekin, Ertuğrul G&ouml;ksoy Background: The appropriate position that will be given to patient is important to prevent hematoma related pulmonary complications and provide comfort in early postoperative period of thyroidectomy patients (1,2). Aim: This study was carried out to determine the effects of bed head slope on patients&rsquo; outcomes in early postoperative period after thyroidectomy. Methodology: The sample of this randomized controlled trial included 114 thyroidectomy patients in a university hospital from May 2013 to September 2013. By randomization, the patients were divided into three groups including 38 patients in each. Their bed head slopes were provided in three levels (0&deg; flat, 30&deg; low-fowler and 45&deg; high fowler positions). In early postoperative period of thyroidectomy, outcomes of patients were evaluated at 1st, 2nd, 3rd, and 4th hours. Data were analyzed using number, percentage, mean, standard deviation, Pearson&rsquo;s chi-square, Kruskal Wallis, Friedman and advanced analysis tests. Results: The mean age of patients was 46.61&plusmn;15.28. The majority of the patients were female (83.3%) and 84.2% had underwent total thyroidectomy. There was statistical significance among the three groups for patients&rsquo; amount of drainage at 1st, 3rd, and 4th hours after thyroidectomy. However, amount of drainage of patients in flat group was significantly higher compared to high fowler group at 2nd hour. No hematoma was seen in any of the patients. Dyspnea did not occur in any of the patients in low fowler group. The majority of patients with breathing failure had bed head levels of &ldquo;0&rdquo; degree in 2nd and 3rd hours after thyroidectomy. Conclusion: The findings of this study showed that affected breathing and amount of drainage of thyroidectomy patients of different positions during early postoperative period. Implications of perioperative nursing: Flat, low fowler and high fowler positions were safe and, low fowler position was more comfortable for the thyroidectomy patients in early postoperative period. Key Words: Bed head slope, Nursing care, Postoperative period, Position, Thyroidectomy Reference 1 Terris DJ, Snyder S, Carneiro-Pla D, Inabnet WB 3rd, Kandil E, Orloff L, Shindo M, Tufano RP, Tuttle RM, Urken M, Yeh MW, American Thyroid Association Surgical Affairs Committee Writing Task Force. American Thyroid Association Statement on outpatient thyroidectomy. Thyroid, 2013; 23: 1193-202. 2 Rosato L, De Crea C, Bellantone R, Brandi ML, De Toma G, Filetti S, Miccoli P, Pacini F, Pelizzo MR, Pontecorvi A, Avenia N, De Pasquale L, Chiofalo MG, Gurrado A, Innaro N, La Valle G, Lombardi CP, Marini PL, Mondini G, Mullineris B, Pezzullo L, Raffaelli M, Testini M, De Palma M. Diagnostic, therapeutic and health-care management protocol in thyroid surgery: a position statement of theItalian Association of Endocrine Surgery Units (U.E.C. CLUB). J Endocrinol Invest, 2016, Apr 8. *Presenter author: Assistant Professor Serpil Y&uuml;ksel, Ph.D, R.N Konya Necmettin Erbakan University, Faculty of Health Science, Division of Nursing, Department of Surgical Nursing, Konya, Turkey. *Contact Author: Serpil Y&uuml;ksel Affiliation: Konya Necmettin Erbakan University, Faculty of Health Science, Division of Nursing, Department of Surgical Nursing, Konya, Turkey. Postal address: Necmettin Erbakan &Uuml;niversitesi, Sağlık Bilimleri Fak&uuml;ltesi, Cerrahi Hastalıkları Hemşireliği Anabilim Dalı, İhsaniye Mah. Kazım Karabekir Cad. 42040. Sel&ccedil;uklu/Konya, Turkey. Office phone: +90-(0)332 3204049 (2029 ext.), Office fax: +90-(0)332-3204059 E-mail: [email protected]

Validity and reliability of the Turkish version of the DSM-5

The goal of this study was to assess the validity and reliability of the Turkish version of the DSM-5 (Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition) Dissociative Symptoms Severity Scale-Child Form. The scale was prepared by translating and then back-translating the DSM-5 Dissociative Symptoms Severity Scale. The study groups included one group of 30 patients diagnosed with posttraumatic stress disorder who were treated in a child and adolescent psychiatry unit and another group of 83 healthy volunteers from middle and high schools in the community. For assessment, the Adolescent Dissociative Experiences Scale (ADES) was used in addition to the DSM-5 Dissociative Symptoms Severity Scale. Regarding the reliability of the DSM-5 Dissociative Symptoms Severity Scale, Cronbach's alpha was .824 and item-total score correlation coefficients were between .464 and .648. The test-retest correlation coefficient was calculated to be r=.784. In terms of construct validity, one factor accounted for 45.2% of the variance. Furthermore, in terms of concurrent validity, the scale showed a high correlation with the ADES. In conclusion, the Turkish version of the DSM-5 Dissociative Symptoms Severity Scale-Child Form is a valid and reliable tool for both clinical practice and research