Traumatic abdominal wall hernias: disruptions of the abdominal wall muscles associated to pelvic bone fractures illustrated by two case reports.

Blunt abdominal traumas are often associated with intra-abdominal injuries and pelvic fractures. Traumatic abdominal wall hernias due to disruption of the abdominal wall muscles may be overlooked. Delayed diagnosis can lead to hernia related complications. We present two cases of high kinetic trauma with pelvic fractures and acute traumatic abdominal wall herniation. Both of these cases suffered from a delayed diagnosis and needed surgery to treat the symptomatic herniation. Clinical reassessment and appropriate medical imaging are mandatory in patients with high kinetic abdominal blunt traumas and associated pelvic fracture, in order to prevent delayed diagnosis and possible complications

Analysis of genetic variation among accessions of critically endangered Rhaponticoides iconiensis and Rhaponticoides mykalea based on RAPD and SDSPAGE markers

Rhaponticoides iconiensis (Hub.-Mor.) M.V.Agab and Greuter is a rare and endangered endemic species of the Konya region of Turkey. One related taxon, Rhaponticoides mykalea (Hub.-Mor.) M.V.Agab andGreuter, is morphologically similar but occurs in different geographical locations. This study has been conducted on the biology of this threatened plant in order to understand better the factors that shouldbe included in the development of conservation practices. The genetic variations were studied using RAPD markers and SDS-PAGE profiles of total seed proteins for three R. iconiensis populations and two R. mykalea populations. The analyzed R. iconiensis populations belonged to different soil types (calcareous and volcanic). The analyzed R. iconiensis and R. mykalea populations belonged to different bioclimatic zones. A genetic diversity within populations was detected both by SDS-PAGE and RAPD for R. iconiensis populations. The level of variation did not differ with respect to soil type for the species studied. Populations collected from the same soil types carried more polymorphisms than those grown in different zones. The genetic diversity was revealed more clearly for all populations byRAPD than through analyzing proteins. Differentiation between ecological groups was higher than that revealed within groups. Conservation programs should take into account the level of genetic diversity within population revealed by these markers according to soil types

Effect of micronized zeolite addition to lamb concentrate feeds on growth performance and some blood chemistry and metabolites

This study was conducted to determine the effects of the addition of micronized zeolite (MZ) on the fattening performance, blood parameters, faecal ash and nitrogen levels of lambs fed concentrate feeds intensively. For two months 25 four-month-old Merino x Ile de France crossbred male lambs (21.1 ± 1.32 kg live weight) were fed 100 g alfalfa hay and a mixed concentrate diet containing 0%, 1%, 2% or 3% additional MZ. At the end of the study, bodyweight gain and feed consumption were not affected by the treatments. Similarly, the addition of up to 2% MZ to the diet did not affect slaughter weight, hot carcass or cold carcass weights, but they decreased at 3% MZ inclusion. No differences were observed between the groups in terms of blood urea nitrogen, plasma glucose, serum creatinine, triglyceride, sodium, potassium and chlorine concentration. However, serum total protein, calcium and phosphorus concentrations were affected by MZ supplementation. The addition of MZ to the ration did not affect the faecal dry matter content and total nitrogen level, yet it increased the ash content of the faeces. Consequently, it was demonstrated that the addition of up to 2% MZ to lamb grower feed does not have a negative impact on performance and carcass yield of the animals, but affects serum total protein, calcium and phosphorus concentrations

Brieskorn manifolds as contact branched covers of spheres

We show that Brieskorn manifolds with their standard contact structures are contact branched coverings of spheres. This covering maps a contact open book decomposition of the Brieskorn manifold onto a Milnor open book of the sphere.Comment: 8 pages, 1 figur

Rare hemoglobin variant Hb Yaizu observed in Turkey

Objective: To determine the characteristic features of the rare hemoglobin (Hb) variant Hb Yaizu to enable laboratory diagnosis of the hemoglobin variants during screening programs. Materials and Methods: Genomic DNA was obtained from the 4 members of a family living in Denizli province, an Aegean region of Turkey. Blood cell counts, hemoglobin composition, hemoglobin electrophoresis (both alkaline and acid), HPLC analysis, DNA sequencing and beta globin gene cluster haplotypes were done. Results: Hb Yaizu carriers were apparently healthy individuals. Hb Yaizu was slightly faster than Hb S at alkaline pH, but slower than Hb S at acidic pH in hemoglobin electrophoresis. An abnormal hemoglobin peak was observed with a retention time of 4.77 min in HPLC analysis attributed to Hb Yaizu. Two members of the family were heterozygous Hb Yaizu [beta 79(EF3) Asp>Asn] confirmed by DNA sequencing. The mutation was found to be linked with the Mediterranean haplotype I [+ - - ++]. Conclusion: We have presented the details of Hb Yaizu, a rare hemoglobin variant that may be important to hemoglobinopathy screening programs, although its clinical significance is unclear. Copyright © 2008 S. Karger AG

L-MYC gene polymorphism and risk of thyroid cancer

L-myc gene polymorphism is a representative genetic trait responsible for an individual’s susceptibility to several cancers. However, there have been no reports concerning the association between thyroid cancer and L-myc gene polymorphism. Aim: To analyze the distribution of L-myc gene polymorphism in Turkish patients with thyroid disorders and thyroid cancers. Methods: We used a molecular genotyping method, polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP). We studied 138 patients of whom 47 had multinodular goiter, 13 had follicular cancer and 69 had papillar cancer, in comparison with control group of 109 healthy individuals. Results: No significant difference in the distribution of genotypes was observed between thyroid patients and controls. Carrying SS or LS genotype revealed a 1.96-fold (95% CI 0.573–6.706) risk for the occurrence of follicular cancer when compared with controls, and 3.11-fold (95% CI 0.952–10.216), when compared with multinodular goiter patients (p = 0.04). Conclusion: We suggest that L-myc genotype profiling together with other susceptibility factors, may be useful in the screening for thyroid nodular malignancy.Для ряда опухолей человека показана корреляция между риском развития опухоли и определенным вариантом гена L-MYC. Данные о наличии такой связи при раке щитовидной железы к настоящему времени отсутствуют. Цель: проанализировать распределение полиморфных типов гена L-MYC в популяции больных с доброкачественными и злокачественными поражениями щитовидной железы, включая рак щитовидной железы, в Турции. Методы: для анализа полиморфизма гена L-MYC использован метод молекулярного генотипирования, в частности, метод определения полиморфизма длины рестрикционных фрагментов, основанный на полимеразной цепной реакции (PCR-RFLP). Определение проводили в лейкоцитах 138 больных, в том числе 48 больных с узловым зобом, 13 больных фолликулярным раком щитовидной железы и 69 больных папиллярным раком. Контрольную группу составляли 109 здоровых лиц. Результаты: статистически достоверных различий в распределении исследуемых генотипов у больных с патологией щитовидной железы и здоровых лиц не выявили. Показано, что относительный риск фолликулярного рака щитовидной железы у больных-носителей генотипа SS или LS составляет 1,96 по сравнению со здоровыми лицами (при 95% доверительном интервале от 0,573 до 6,706) и 3,11 по сравнению с больными с узловым зобом (при 95% доверительном интервале от 0,952 до 10,216) (р = 0,04). Выводы: по нашему предположению, определение профиля полиморфизма гена L-MYC с учетом других факторов, определяющих предрасположенность к развитию опухолей, может быть полезным при скрининге озлокачествления узелковых образований щитовидной железы

Biofortification and Localization of Zinc in Wheat Grain

Zinc (Zn) deficiency associated with low dietary intake is a well-documented public health problem, resulting in serious health and socioeconomic problems. Field experiments were conducted with wheat to test the role of both soil and foliar application of ZnSO4 in Zn concentration of whole grain and grain fractions (e.g., bran, embryo and endosperm) in 3 locations. Foliar application of ZnSO4 was realized at different growth stages (e.g., stem elongation, boot, milk, dough stages) to study the effect of timing of foliar Zn application on grain Zn concentration. The rate of foliar Zn application at each growth stage was 4 kg of ZnSO4 3 7H2O ha-1. Laser ablation (LA)-ICP-MS was used to follow the localization of Zn within grain. Soil Zn application at a rate of 50 kg of ZnSO4 3 7H2O ha-1 was effective in increasing grain Zn concentration in the Zn-deficient location, but not in the locations without soil Zn deficiency. In all locations, foliar application of Zn significantly increased Zn concentration in whole grain and in each grain fraction, particularly in the case of high soil N fertilization. In Zn-deficient location, grain Zn concentration increased from 11 mg kg-1 to 22 mg kg-1 with foliar Zn application and to 27 mg kg-1 with a combined application of ZnSO4 to soil and foliar. In locations without soil Zn deficiency, combination of high N application with two times foliar Zn application (e.g., at the booting and milk stages) increased grain Zn concentration, on average, from 28 mg kg-1 to 58 mg kg-1. Both ICP-OES and LA-ICP-MS data showed that the increase in Zn concentration of whole grain and grain fractions was pronounced when Zn was sprayed at the late growth stage (e.g., milk and dough). LA-ICP-MS data also indicated that Zn was transported into endosperm through the crease phloem. To our knowledge, this is the first study to show that the timing of foliar Zn application is of great importance in increasing grain Zn in wheat, especially in the endosperm part that is the predominant grain fraction consumed in many countries. Providing a large pool of Zn in vegetative tissues during the grain filling (e.g., via foliar Zn spray) is an important practice to increase grain Zn and contribute to human nutritio

Subcutaneous hydatid cysts occurring in the palm and the thigh: two case reports

<p>Abstract</p> <p>Introduction</p> <p>Hydatid cyst disease is common in some regions of the world and is usually located in the liver and lungs. This report presents two cases of primary hydatid cysts located subcutaneously: one in the medial thigh and one in the left palm between the index and middle fingers.</p> <p>Case presentations</p> <p>A 64-year-old male farmer visited our hospital because a swelling on the right medial thigh had grown during the last year. Superficial ultrasound and computed tomography revealed a lesion resembling a hydatid cyst. A germinative membrane was encountered during surgical excision. Pathological examination was compatible with a hydatid cyst. The second case involved a 67-year-old male farmer who complained of a swelling that had grown in his left palm in the last year. The preliminary diagnosis was a lipoma. However, a hydatid cyst was diagnosed during surgical excision and after the pathological examination. The patient did not have a history of hydatid cyst disease and hydatid cysts were not detected in other organs. There has been no disease recurrence after following both patients for 3 years.</p> <p>Conclusion</p> <p>A hydatid cyst should be considered in the differential diagnosis of subcutaneous cystic lesions in regions where hydatid cysts are endemic, and should be excised totally, with an intact wall, to avoid recurrence.</p

Formation of different magnetic phases and high Curie temperature ferromagnetism in Fe57-implanted ZnO film

© 2014 Elsevier B.V. All rights reserved. We investigated magnetic properties of ZnO thin film implanted with Fe57 ions to the fluence of 1.00×1017 ions/cm2. Both vibrating sample magnetometry and magneto-optical Kerr effect measurements revealed strong room temperature ferromagnetism with similar hysteresis loops. Temperature dependent measurements showed a very high Curie temperature around 850 K. Conversion electron Mössbauer spectroscopy experiments proved the existence of paramagnetic Fe+3 and Fe+2 ions and also the presence of substitutional Fe atoms in the hexagonal ZnO crystal resulting in intrinsic ferromagnetic order