Anticholinesterases

Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) are known serine hydrolase enzymes responsible for the hydrolysis of acetylcholine (ACh). Although the role of AChE in cholinergic transmission is well known, the role of BChE has not been elucidated sufficiently. The hydrolysis of acetylcholine in the synaptic healthy brain cells is mainly carried out by AChE; it is accepted that the contribution to the hydrolysis of BChE is very low, but both AChE and BChE are known to play an active role in neuronal development and cholinergic transmission. Myasthenia gravis (MG) is a muscle disease characterized by weakness in skeletal muscles and rapid fatigue. Anticholinesterases, which are not only related to the immune origin of the disease but also have only symptomatic benefit, have an indispensable role in the treatment of MG. Pyridostigmine, distigmine, neostigmine, and ambenonium are the standard anticholinesterase drugs used in the symptomatic treatment of MG. All of these compounds may increase the response of the myasthenic muscle to recurrent nerve impulses, primarily by protecting the endogenous ACh

Synthesis and cytotoxicity studies on new pyrazolecontaining oxime ester derivatives

Purpose: To synthesize a series of new 1-(2-naphthyl)-2-(1H-pyrazol-1-yl)ethanone oxime ester derivatives (5-12) with potential anticancer properties, and to determine their cytotoxic effects in mouse fibroblast and human neuroblastoma cell lines. Methods: The title compounds were obtained through sodium salt reaction of 1-(naphthalene-2-yl)-2- (1H-pyrazol-1-yl)etanone oxime (4) with various acyl chlorides. The cytotoxic effects were evaluated by MTS colorimetric assay, while physicochemical descriptors were calculated using QikProp software. Results: Most of the compounds showed approximately 50 – 60 % inhibition against SH-SY5Y neuroblastoma cells at 100 μM. Of these, compound 7a was the most active combination with an IC50 value of 85.94 µM. The toxic effect of the compounds on mouse fibroblast cell line was insignificant (p < 0.05) even when the dose was increased. The calculated physicochemical properties of the compounds were within drug-like chemical space. Conclusion: The synthesized oxime ester derivatives with pyrazole ring exhibit selective toxicity to neuroblastoma cells without affecting healthy mouse fibroblast cells. The compounds proved to be druglike while their pharmacokinetic features were also encouraging, and were in line with in silico predictions

Model-based testing in practice: An experience report from the web applications domain

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Comparison of Satisfaction of Body Parts and the Success Levels of the Teams That Participated in Universities Handball 2nd League Competitions

The study was conducted to determine and compare the satisfaction levels of the students participating in the Universities 2nd Handball League competitions on body parts and body features and the success of their teams. The study group consisted of n=213 (111 female, 102 male) students between 18-24 years of age who participated in 2016-2017 Universities 2nd Handball League competitions (8 Female Teams, 8 Male Teams). The “Body Parts and Body Features Satisfaction Scale” was used as the measurement tool. There are 26 items for women and 27 items for men in this scale. The One-Way Variance Analysis and Tukey Test were used in the comparison of the satisfaction scores of the male handball players from their body parts with the success levels in competitions, and the Kruskal Wallis Test and Mann Whitney U-Test were used in female participants.According to the satisfaction from body parts and features average scores, the differences between the male and female teams were found to be statistically significant (p<0,05). These differences were detected in İbrahim Çeçen, Erzincan and Doğu Akdeniz University teams in men; and in Atatürk, Sütçü İmam and Erciyes University teams in women.As a result, although there was a statistically significant difference between the average satisfaction scores of the teams, which were the 1st and the last in the tournament, in terms of body parts and features, it was also determined that this situation was not influential on the success rating of the teams

Synthesis, crystal structure and spectroscopic studies of a new silver complex derived from [1-(3-pyridinyl) ethanone]

In this work, a new silver (I) complex, [Ag(3-pye)2(H2O)](NO3) where 3-pye=1-(3-pyridinyl) ethanone, has been synthesized and characterized by elemental analyses, FT-IR, fluorescence spectroscopy, and single X-ray crystallography. The X-ray diffraction analysis revealed that the Ag(I) complex crystallized in the monoclinic system with the C2/c space group. Computational studies were performed using DFT approache on the present complex to get insight into the structural parameters, spectral characteristics and electronic properties. The characterization results were found to be consistent with the proposed structure of the complex, and the DFT approach supported the experimental results. Also, Hirshfeld surface analysis was used to identify the non-covalent interactions within the crystal structure as well as to visualize the conformity of the crystal structure

Evaluating the home isolation of COVID-19 patients in primary care

Background: This study aims to evaluate the effectiveness of home isolation and medical follow-up by analyzing data collected over the phone from isolated individuals. Methods: A cross-sectional phone-based survey designed to evaluate the home isolated COVID-19 suspected patients at the Şişli Hamidiye Etfal Family Health Center in Istanbul city between 16th&nbsp; March&nbsp; 5th May 2020.&nbsp; A semi-structured questionnaire and the universal sampling technique were recruited to collect data about the socio-demographic and the COVID-19 related laboratory and clinical findings. The SPSS for Windows program was used to perform a univariate and bivariate statistical analysis. The Statistical alpha significance level was accepted at less than 0.05. Results: A total of 463 confirmed, probable, or suspected cases of COVID-19 took part in this study with a mean age of 35.38 ∓17.1 (range: 0-86 years). Tow-third 310 (67.0%) underwent the PCR tests, and 67 (21.6%) confirmed positive results. Moreover, one-third (159, 34.3%) exposed to CT scans; however, 51(32.3%) were compatible with COVID-19. The median age of individuals with PCR positive was 38 years. More than half (40, 59.7%) were males, compared to 27 (40.3%) were females. There was no significant relationship between PCR positivity and pandemic period, age, or gender (P = 0.149; P = 0.545; P = 0.285), respectively. Although older individuals had a higher rate of CT scan compatible with COVID-19, the relation between increased age and COVID-19 compatible CT was found not to be statistically significant (P = 0.053). Moreover, there was&nbsp; significant relationship between CT scan positivity and coughing, the tobacco smoking and diabetes (P = 0.003; P = 0.032; P = 0.016),&nbsp; respectively. Conclusion: Combining PCR, symptoms, and CT together doubles the likelihood of a correct diagnosis. Quarantined patients must be regularly monitored

MRI of lower extremity impingement and friction syndromes in children

Although generally more common in adults, lower extremity impingement and friction syndromes are also observed in the pediatric age group. Encompassing femoroacetabular impingement, iliopsoas impingement, subspine impingement, and ischiofemoral impingement around the hip; patellar tendon–lateral femoral condyle friction syndrome; iliotibial band friction syndrome; and medial synovial plica syndrome in the knee as well as talocalcaneal impingement on the hindfoot, these syndromes frequently cause pain and may mimic other, and occasionally more ominous, conditions in children. Magnetic resonance imaging (MRI) plays a key role in the diagnosis of musculoskeletal impingement and friction syndromes. Iliopsoas, subspine, and ischiofemoral impingements have been recently described, while some features of femoroacetabular and talocalcaneal impingements have recently gained increased relevance in the pediatric population. Fellowship-trained pediatric radiologists and radiologists with imaging workloads of exclusively or overwhelmingly pediatric patients (particularly those without a structured musculoskeletal imaging program as part of their imaging training) specifically need to be aware of these rare syndromes that mostly have quite characteristic imaging findings. This review highlights MRI features of lower extremity impingement and friction syndromes in children and provides updated pertinent pathophysiologic and clinical data