Hematoma cervical epidural com fratura de clivus: relato de caso

Clivus fractures are rare and severe entities, usually associated with vascular or cranial nerve lesions and frequently diagnosed postmortem. Cervical epidural haematomas can be traumatic or spontaneous, manifested in acute or chronic form, and are treated surgically in the majority of cases, although the conservative treatment also can be indicated to patients with incomplete and non-progressive deficits. The authors report the case of a female patient, 8 years old, victim of trampling in public way by a high velocity motorized vehicle, admitted in Glasgow 7, anisocoric pupils (left pupil midriatic), whose radiological investigation showed a transverse fracture of the clivus, cervical epidural haematoma and diffuse axonal injury. The patient was submitted to intracranial pressure monitorization, sedation and conservative treatment with dexamethasone, with good outcome. The authors also present a literature review.As fraturas de clivus são entidades raras e graves, usualmente associadas a lesões vasculares ou de nervos cranianos, sendo freqüentemente diagnosticadas postmortem. Hematomas epidurais cervicais podem ser traumáticos ou espontâneos, manifestos de forma aguda ou crônica, requerendo tratamento cirúrgico na maior parte das vezes, embora o tratamento conservador possa ser indicado a pacientes com déficits incompletos ou não progressivos. Os autores relatam o caso de uma paciente do sexo feminino, 8 anos, vítima de atropelamento em via pública por veículo automotor em alta velocidade, que foi admitida em glasgow 7, com anisocoria (pupila esquerda midriática). A investigação radiológica evidenciou fratura transversa de clivus, hematoma epidural cervical e lesão axonal difusa. A paciente foi submetida a monitorização da pressão intracraniana, sedação e tratamento conservador com corticoesteróides, com boa evolução. Os autores apresentam também uma revisão da literatura pertinente.Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Neurosurgery DepartmentUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de MedicinaUNIFESP, EPM, Neurosurgery DepartmentUNIFESP, EPMSciEL

Characteristics and Analyses of Strategic Factors in Reverse Channels of Açaí Pulp Production Waste in the Municipality of Castanhal/PA/ Caracterização e análise de fatores estratégicos nos canais reversos do resíduo da produção de polpa de açaí no município de Castanhal-PA

When disposed of inappropriately, waste from the production of açaí pulp, originating from the açaí production chain, is the cause of many environmental, economic, and social problems. In an attempt to minimize impact, Reverse Logistics is an instrument of economic, environmental, and social development characterized by actions aimed at the treatment and reuse of waste, which is managed and moved through Reverse Channels. Consulted bibliography defines Strategic Factors capable of promoting the structuring and organization of waste for environmentally appropriate destination by different production sectors. This research analyzed economic, environmental/ecological, technological, logistical, and legislative strategic factors of the reverse channels for açaí production waste in the city of Castanhal/PA, as this municipality’s açaí pulp production is nationally relevant. For this purpose, it was necessary first to characterize local reverse channels, since bibliographic and documentary research did not yield relevant information on the subject. Field research—consisting of in loco observation, structured interviews, and application of online questionnaires to agents directly and indirectly involved—was fundamental to characterize the object of this study. As a result, we achieved the characterization of reverse channels, as well as their logistical resources and the stakeholders involved, starting from the generation of waste through to transportation and final destination, ending our analysis with the main forms of waste revaluation. Our research also allowed the identification and analysis of strategic factors capable of influencing the structuring and maintenance of activities in this reverse chain

Retenção de corpos estranhos: revisão integrativa de literatura / Foreign body retention: an integrative literature review

Objetivo: Analisar artigos científicos publicados em periódicos on-line no cenário nacional e internacional acerca da temática retenção de instrumentos cirúrgicos/corpos estranhos, seus riscos e prevenção. Métodos: Revisão integrativa de literatura, com coleta de dados no mês de agosto de 2018, na biblioteca MEDLINE/PubMed. Resultados: Foram identificadas 14 publicações cujas análises textuais permitiram a produção das seguintes abordagens temáticas: I) Causas e consequências da retenção de corpos estranhos em procedimento cirúrgico II) Métodos de prevenção para com a retenção de corpos estranhos em procedimentos cirúrgicos. Conclusão: Evidências mostram a importância do cuidado e da prevenção do esquecimento de instrumentos cirúrgicos/ corpos estranhos em cirurgias e a necessidade de os profissionais estarem atentos na redução da ocorrência desses casos, para evitar complicações para os pacientes.

Osmoprotectants play a major role in the Portulaca oleracea resistance to high levels of salinity stress—insights from a metabolomics and proteomics integrated approach

IntroductionPurslane (Portulaca oleracea L.) is a non-conventional food plant used extensively in folk medicine and classified as a multipurpose plant species, serving as a source of features of direct importance to the agricultural and agri-industrial sectors. This species is considered a suitable model to study the mechanisms behind resistance to several abiotic stresses including salinity. The recently achieved technological developments in high-throughput biology opened a new window of opportunity to gain additional insights on purslane resistance to salinity stress—a complex, multigenic, and still not well-understood trait. Only a few reports on single-omics analysis (SOA) of purslane are available, and only one multi-omics integration (MOI) analysis exists so far integrating distinct omics platforms (transcriptomics and metabolomics) to characterize the response of purslane plants to salinity stress.MethodsThe present study is a second step in building a robust database on the morpho-physiological and molecular responses purslane to salinity stress and its subsequent use in attempting to decode the genetics behind its resistance to this abiotic stress. Here, the characterization of the morpho-physiological responses of adult purslane plants to salinity stress and a metabolomics and proteomics integrative approach to study the changes at the molecular level in their leaves and roots is presented.Results and discussionAdult plants of the B1 purslane accession lost approximately 50% of the fresh and dry weight (from shoots and roots) whensubmitted to very high salinity stress (2.0 g of NaCl/100 g of the substrate). The resistance to very high levels of salinity stress increases as the purslane plant matures, and most of the absorbed sodium remains in the roots, with only a part (~12%) reaching the shoots. Crystal-like structures, constituted mainly by Na+, Cl−, and K+, were found in the leaf veins and intercellular space near the stoma, indicating that this species has a mechanism of salt exclusion operating on the leaves, which has its role in salt tolerance. The MOI approach showed that 41 metabolites were statistically significant on the leaves and 65 metabolites on the roots of adult purslane plants. The combination of the mummichog algorithm and metabolomics database comparison revealed that the glycine, serine, and threonine, amino sugar and nucleotide sugar, and glycolysis/gluconeogenesis pathways were the most significantly enriched pathways when considering the total number of occurrences in the leaves (with 14, 13, and 13, respectively) and roots (all with eight) of adult plants; and that purslane plants employ the adaptive mechanism of osmoprotection to mitigate the negative effect of very high levels of salinity stress; and that this mechanism is prevalent in the leaves. The multi-omics database built by our group underwent a screen for salt-responsive genes, which are now under further characterization for their potential to promote resistance to salinity stress when heterologously overexpressed in salt-sensitive plants

Imposex in endemic volutid from northeast Brazil (Mollusca: Gastropoda)

Imposex is characterized by the development of masculine sexual organs in neogastropod females. Almost 120 mollusk species are known to present imposex when exposed to organic tin compounds as tributyltin (TBT) and triphenyltin (TPT). These compounds are used as biocide agents in antifouling paints to prevent the incrustations on boats. Five gastropod species are known to present imposex in Brazil: Stramonita haemastoma, Stramonita rustica, Leucozonia nassa, Cymathium parthenopeum and Olivancillaria vesica. This paper reports the first record of imposex observed in the endemic gastropod Voluta ebraea from Pacheco Beach, Northeast Brazil. Animals presenting imposex had regular female reproductive organs (capsule gland, oviduct and sperm-ingesting gland) and an abnormal penis. As imposex occurs in mollusks exposed to organotin compounds typically found at harbors, marinas, shipyards and areas with high shipping activities, probably contamination of Pacheco Beach is a consequence of a shipyard activity located in the nearest areas.O imposex caracteriza-se pelo surgimento de estruturas sexuais masculinas, em fêmeas de gastrópodes. Cerca de 120 espécies de moluscos que exibem o fenômeno quando expostas a contaminação por compostos orgânicos de estanho tais como o Tributilestanho (TBT) e o Trifenilestanho (TPT). Esses compostos são utilizados, sobretudo em embarcações, no intuito de evitar a bioincrustração que danifica as embarcações e eleva os custos das viagens marítimas. No Brasil se conhecem 5 espécies de moluscos gastrópodes que manifestam imposex, são elas: Stramonita haemastoma, Stramonita rustica, Leucozonia nassa, Cymathium parthenopeum e Olivancillaria vesica. No Nordeste, monitoramentos da contaminação por organoestânicos foram realizados utilizando o imposex em gastrópodes como biomarcador. O presente estudo tem por objetivo notificar a primeira ocorrência de imposex na espécie endêmica do Nordeste brasileiro, Voluta ebraea. De um total de 11 animais observados, duas fêmeas apresentaram imposex, provenientes da Praia do Pacheco no litoral do Ceará. Observou-se nesses indivíduos a presença de glândula de cápsulas, ovidutos e receptáculo seminal concomitantemente ao pênis o que caracteriza o imposex. Como o imposex só se manifesta em moluscos expostos a compostos organoestânicos tipicamente encontrados em portos, marinas, estaleiros e locais com grande fluxo de embarcações atribui-se a origem dessa contaminação provavelmente a um estaleiro localizado nas proximidades da área de coleta

The Increasing Incidence of Imposex in Stramonita Haemastoma(Mollusca: Gastropoda: Muricidae) After the Establishment of the Pecém Harbor, Ceará State, Northeast Brazil

Imposex is characterized by the development of masculine sexual organs in neogastropod females,caused by organotin contamination of the seawater. These compounds,as tributyltin (TBT), are used world-wide as biocidal agents in antifouling paints, commonly used to covering boats and off shore metallic structures. Five sampling sites were determined along the coast of Ceará State, Northeast Brazil in March 2001. In each site were collected 50 adults of neogastropod Stramonita haemastoma and indexes that quantify imposex (% imposex, RPLI,RPSI and VDSI) were observed. Other two collecting times were conducted (April 2003 and March 2004) at the same sites and imposex indexes were observed again. The indexes observed in the three collecting times showed an increase in the incidence of imposex at the sampling sites, probably caused by the establishment of Pecém Harbor and its increasing shipping activities

Imposex in Three Prosobranch Species from Ilha do Japonês, Rio de Janeiro, Southeast Brazil

Imposex in gastropods is used worldwide as a bioindicator of organotin compounds (OTs). Samples of three prosobranch mollusc species were collected(Stramonita haemastoma, Leucozonia nassa and Cymatium parthenopeum) in the Ilha do Japonês tidal flat, Rio de Janeiro, Southeast Brazil. The individuals were led to the laboratory and were examined for occurrence of imposex. Imposex was observed and described for each species: S. haemastoma showed the higher indexes (% imposex, RPSI and RPLI) followed by L. nassa and C. parthenopeum. The results suggest that S. haemastoma is the most indicate species to organotin pollution monitoring at Ilha do Japonês, Brazil