Potential of the essential oil from Pimenta pseudocaryophyllus as antimicrobial agent

This study evaluated the effectiveness of the essential oil of Pimenta pseudocaryophyllus in inhibiting the growth of the main bacteria responsible for bad perspiration odor (Staphylococcus epidermidis, Proteus hauseri, Micrococcus yunnanensis and Corynebacterium xerosis). The chemical profile of the essential oil was evaluated by high-resolution gas chromatography (HR-GC) and four constituents were identified, eugenol being the major component (88.6 %). The antimicrobial activity was evaluated by means of the turbidimetric method, using the microdilution assay. The minimum inhibitory concentration (MIC) values of the essential oil ranged from 500 to 1.000 μg mL–1. Scanning electron microscope (SEM) observations confirmed the physical damage and morphological alteration of the test bacteria treated with the essential oil, reference drugs and eugenol. The findings of the study demonstrated that this essential oil can be used in the formulation of personal care products

Óleo essencial de Origanum vulgare Linnaeus: uma alternativa contra as principais bactérias causadoras de mau odor

The use of personal care products became a daily concern in the life of ordinary people. However, due to the toxicological effects of some synthetic compounds, consumers are increasingly interested in the use of natural actives. Thus, this study aimed to investigate the antimicrobial potential of essential oil of Origanum vulgare L. against the main bacterial strains responsible for bad perspiration odor (Staphylococcus epidermidis ATCC 12228, Proteus vulgaris ATCC 13315, Micrococcus luteus ATCC 7468 and Corynebacterium xerosis IAL 105) and to develop an antiperspirant deodorant containing only the essential oil of O. vulgare as an antimicrobial agent. The chemical profile of the essential oil was evaluated by high-resolution gas chromatography and the major component was identified γ-terpinene. Bactericidal activity was observed against P. vulgaris ATCC 13315, M. luteus ATCC 7468 and C. xerosis IAL 105, with minimum inhibitory concentration (MIC) values of 1400, 2800 and 1400 μg/mL, respectively and bacteriostatic activity against all of them (MIC=700 μg/mL) and S. epidermidis ATCC 12228 (MIC=2800 μg/mL). The antiperspirant deodorant containing 2% essential oil presented bactericidal activity against all tested bacteria. Electron microscopy images confirmed the physical damage and morphological alteration in the structure of bacteria exposed to the treatments. The development of antiperspirant deodorant formulation containing the essential oil of O. vulgare can be a natural antimicrobial alternative to the use of triclosan.O uso de produtos de higiene pessoal tornou-se preocupação diária na vida das pessoas. No entanto, devido aos comprovados efeitos toxicológicos de alguns compostos sintéticos, os consumidores estão cada vez mais interessados na utilização de ativos naturais. O objetivo desse estudo foi investigar o potencial antimicrobiano do óleo essencial de Origanum vulgare Linnaeus frente às principais bactérias responsáveis pelo mau odor axilar (Staphylococcus epidermidis ATCC 12228, Proteus vulgaris ATCC 13315, Micrococcus luteus ATCC 7468 e Corynebacterium xerosis IAL 105) e desenvolver um desodorante antitranspirante que contenha o óleo essencial de O. vulgare como agente antimicrobiano. Foi avaliado o perfil químico do óleo essencial por cromatografia gasosa de alta resolução (CG-AR) e o componente majoritário identificado foi o γ-terpineno (30,5%). O óleo essencial apresentou atividade bactericida frente ao P. vulgaris ATCC 13315, M. luteus ATCC 7468 e C. xerosis IAL 105 com valores de concentração inibitória mínima (CIM)=1400; 2800 e 1400 μg/mL, respectivamente. Apresentou também atividade bacteriostática com valores de CIM=2800 μg/mL para S. epidermidis ATCC 12228 e de CIM=700 μg/mL para as demais. O desodorante antitranspirante contendo 2% de óleo de O. vulgare demonstrou atividade bactericida frente a todas bactérias testadas. As eletromicrografias das linhagens bacterianas expostas aos tratamentos propostos demostraram alterações na estrutura das bactérias. O desenvolvimento do desodorante antitranspirante contendo o óleo essencial de O. vulgare como ativo natural antimicrobiano, confere uma alternativa ao uso do triclosan.CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superio

Stachytarpheta gesnerioides Cham.: composición química de la fracción hexánica y aceite esencial, actividades antioxidante y antimicrobiana

Submitted by Nuzia Santos ([email protected]) on 2014-07-14T18:35:52Z No. of bitstreams: 1 Stachytarpheta gesnerioides cham.pdf: 207770 bytes, checksum: f804b5249d41da948ce67d7e5c3b61e1 (MD5)Made available in DSpace on 2014-07-14T18:35:52Z (GMT). No. of bitstreams: 1 Stachytarpheta gesnerioides cham.pdf: 207770 bytes, checksum: f804b5249d41da948ce67d7e5c3b61e1 (MD5) Previous issue date: 2012Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Biologia. Laboratório de Genética. Juiz de Fora, MG, BrazilUniversidade Federal de Juiz de Fora. Faculdade de Farmácia e Bioquímica. Núcleo de Identificação e Quantificação Analítica. Juiz de Fora, MG, BrazilUniversidade Federal de Juiz de Fora. Faculdade de Farmácia e Bioquímica. Núcleo de Identificação e Quantificação Analítica. Juiz de Fora, MG, BrazilUniversidade Federal de Juiz de Fora. Faculdade de Farmácia e Bioquímica. Núcleo de Identificação e Quantificação Analítica. Juiz de Fora, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Química de Produtos Naturais. Belo Horizonte, MG, BrazilUniversidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Biologia. Laboratório de Genética. Juiz de Fora, MG, BrazilSe han investigado la composición química de la fracción hexánica (FH) y aceite esencial (AE) de Stachytarpheta gesnerioides (Verbenaceae) por GC-MS, el contenido de fenoles totales y flavonoides. La capacidad antioxidante y actividad antimicrobiana fueron investigadas en cinco extractos de hojas de S gesnerioides. Extractos acuosos y etanolico fueron preparados por la maceración dinámica y extracción continua en Soxhlet con hexano, acetato de etilo y metanol. Las fracciones AE y FH están compuestas principalmente por guaiol. La fracción FH es también rica en α-pineno. El contenido de fenoles totales varió desde 0,85 hasta 22,74 mg de ácido gálico/100 mg de extracto seco (Folin-Ciocalteu). La concentración total de flavonoides varió desde 0,68 hasta 13,65 mg en equivalentes de rutina/100 mg de extracto seco, que se detectó mediante reacción con cloruro de aluminio al 8%. El extracto de acetato de etilo (CI50=9,41g/ml) enseño la más grande actividad antioxidante. Los extractos se encontraron eficazes para inhibir los microorganismos ensayados.In the present study we investigated the chemical composition of hexane fraction and essential oil of Stachytarpheta gesnerioides (Verbenaceae) by GC-MS, total phenol and flavonoid contents. The antioxidant capacity and antimicrobial activity were investigated in five extr acts of leaves of S. gesnerioides. Aqueous and 100% ethanol extracts were prepared by dynamic maceration. Hexane, ethyl acetate and methanol extracts were prepared by Soxhlet extraction. The essential oil (EO) and hexane fraction (HF) are mainly composed by guaiol. Moreover, the HF is also rich in the monoterpene α-pinene. The total phenol content ranged from 0.85 to 22.74 mg gallic acid equivalent /100mg dry extract at Folin–Ciocalteu’s reagent method. The total flavonoid concentration ranged from 0.68 to 13.65 mg rutin equivalent /100mg dry extract, detected using 8% aluminium chloride. The ethyl acetate extract (IC50=9.41 μg/ml) showed the highest antioxidant activity. The extracts were found to be effective to inhibit the microorganisms tested. Keywords: Verbenaceae; Stachytarpheta gesnerioides; chromatography-mass spectrometry; flavonoids; total phenol

In vitro inhibition of adhesion of Escherichia coli strains by Xylitol

The present study aimed to evaluate xylitol's antimicrobial and anti-adherence activities on Escherichia coli (ATCC 8739) and on another clinical strain enteropathogenic E. coli (EPEC). In vitro minimum inhibitory concentration (MIC) test and adhesion assays were performed using 0.5, 2.5 and 5.0% xylitol. It was found that xylitol did not have antimicrobial properties on these strains. The scanning electron microscopy (SEM) demonstrated that the slides treated with xylitol had a significant reduction in the number of bacilli and the inhibition of microbial adhesion was probably the xylitol's mechanism of action. Xylitol could be a possible alternative on the control of E. coli infections

Potential of the Essential Oil from Pimenta Pseudocaryophyllus as an Antimicrobial Agent

This study evaluated the effectiveness of the essential oil of Pimenta pseudocaryophyllus in inhibiting the growth of the main bacteria responsible for bad perspiration odor (Staphylococcus epidermidis, Proteus hauseri, Micrococcus yunnanensis and Corynebacterium xerosis). The chemical profile of the essential oil was evaluated by high-resolution gas chromatography (HR-GC) and four constituents were identified, eugenol being the major component (88.6 %). The antimicrobial activity was evaluated by means of the turbidimetric method, using the microdilution assay. The minimum inhibitory concentration (MIC) values of the essential oil ranged from 500 to 1,000 μg mL-1. Scanning electron microscope (SEM) observations confirmed the physical damage and morphological alteration of the test bacteria treated with the essential oil, reference drugs and eugenol. The findings of the study demonstrated that this essential oil can be used in the formulation of personal care products