The in vitro and in vivo investigation of biological activities and phenolic analysis of Helichrysum plicatum subsp. plicatum

In Turkey, Helichrysum genus is represented by 26 taxa belonging to 20 species in Turkish flora of which 14 ones are endemic to Turkey. The aerial parts of Helichrysum plicatum subsp. plicatum are used kidney stones, kidney and stomach ailments. The extraction procedures and solvents are important step in processing of bioactive constituents from the plant materials. Therefore, the aim of this study was to evaluate in vitro antioxidant, antimicrobial, anti-urease, anticholinesterase and in vivo antiinflammatory activities of Helichrysum plicatum subsp. plicatum different extracts. In addition, the phenolic characterization of the Soxhlet and maceration methanol extracts which showed significant antioxidant, anti-urease, antimicrobial, anti-inflammatory and anticholinesterase activities were performed by HPLC-DAD and LC-MS/MS. In the present study, the Soxhlet methanol extract exhibited strong antioxidant, antimicrobial and anticholinesterase activities than other extracts. The maceration methanol extract showed the strongest anti-urease activity. The Soxhlet methanol and maceration methanol extracts showed in vivo anti-inflammatory activities very close to each other. As a result of this study, chlorogenic acid, dicaffeoylquinic acid, luteolin, luteolin-7-O-glucoside, naringenin-O-hexoside and isoquercitrin compounds were analysed in plant. Therefore, it is thought that methanol extracts can be used as a natural source in the future for food and pharmaceutical industries

Probiyotikler: Biyolojik Terapi

Probiyotikler, yeterli miktarda al›nd›klar›nda kona¤› hastal›klardan korumada ve tedavide yararl›, patojen olmayan canl› mikroorganizmalar olarak tan›mlanmaktad›r. Seçilmifl kökenlerinin kullan›m›na ba¤l› olarak, probiyotiklerin mide ba¤›rsak kanal› enfeksiyonlar›nda iyileflme, laktoz metabolizmas›n›n artt›r›lmas›, serum kolesterolünün düflürülmesi, ba¤›fl›kl›k sisteminin uyar›lmas›, enflamatuvar ba¤›rsak hastal›¤› semptomlar›n›n azalt›lmas› ve Helicobacter pylori enfeksiyonlar›n›n bask›lanmas›nda yarar sa¤lad›klar› birçok çal›flma ile ortaya koyulmufltur. Ayr›ca bilimsel çal›flmalar bunlar›n anti-mutajenik, anti-kanserojen ve ishal önleyici özelliklerini de ortaya koymaktad›r. Probiyotiklerin, konak homeostaz›n› artt›rmak ve insan hastal›klar›n›n tedavisinde sahip olduklar› potansiyelleri ortaya koymak konusunda son zamanlarda yap›lan ciddi araflt›rma bulgular› bu yaz›da derlenmifltir

In vitro effectiveness of colistin, tigecycline and levofloxacin alone and combined with clarithromycin and/or heparin as lock solutions against embedded Acinetobacter baumannii strains

Objectives: To determine the in vitro stability and efficacy of colistin, tigecycline and levofloxacin alone or in combination with clarithromycin and/or heparin as lock solutions against biofilm-embedded Acinetobacter baumannii strains