70 research outputs found
Estudio de los factores que determinan la respuesta de adaptación ácida y de protección cruzada frente al calor de Salmonella Typhimurium y Salmonella Senftenberg: mecanismos implicados = Factors influencing the acid tolerance and cross-protection responses of "Salmonella" Typhimurium and "Salmonella" Senftenberg: mechanisms involved
230 p.Este trabajo de investigación se ha centrado en determinar la respuesta frente al estrés ácido de dos serovariedades del género Salmonella, de gran interés sanitario y tecnológico, S. Typhimurium y S. Senftenberg, en presencia de diversos ácidos orgánicos de uso habitual en la industria alimentaria.
Los resultados obtenidos han permitido demostrar que la adaptación de ambas serovariedades en ambientes ácidos moderados origina respuestas adaptativas de tolerancia ácida, que se manifiestan por un incremento de su subsecuente resistencia a condiciones ácidas letales, y de protección cruzada frente a tratamientos térmicos, tanto en medios de laboratorio como en zumos de naranja y manzana. Asimismo hemos comprobado que diversos parámetros ambientales, como la temperatura de crecimiento, la composición del medio de cultivo y tratamiento, o el tipo de ácido utilizado, condicionan la expresión y la magnitud de dichas respuestas adaptativa
Whole Resistome Analysis in Campylobacter jejuni and C. coli Genomes Available in Public Repositories
-Campylobacter spp. are the most frequent agent of human gastroenteritis worldwide,
and the spread of multidrug-resistant strains makes the clinical treatment difficult. The
current study presents the resistome analysis of 39,798 Campylobacter jejuni and
11,920 Campylobacter coli genomes available in public repositories. Determinants of
resistance to β-lactams (Be) and tetracyclines (Te) were the most frequent for both
species, with resistance to quinolones (Qu) as the third most important on C. jejuni
and to aminoglycosides (Am) on C. coli. Moreover, resistance to Te, Qu, and Am
was frequently found in co-occurrence with resistance to other antibiotic families.
Geographical differences on clonal complexes distribution were found for C. jejuni and
on resistome genotypes for both C. jejuni and C. coli species. Attending to the resistome
patterns by isolation source, three main clusters of genomes were found on C. jejuni
genomes at antimicrobial resistance gene level. The first cluster was formed by genomes
from human, food production animals (e.g., sheep, cow, and chicken), and food (e.g.,
dairy products) isolates. The higher incidence of tet(O), associated with tetracycline
resistance, and the gyrA (T86I) single-nucleotide polymorphism (SNP), associated with
quinolone resistance, among genomes from this cluster could be due to the intense use
of these antibiotics in veterinary and human clinical settings. Similarly, a high incidence
of tet(O) genes of C. coli genomes from pig, cow, and turkey was found. Moreover, the
cluster based on resistome patterns formed by C. jejuni and C. coli genomes of human,
turkey, and chicken origin is in agreement with previous observations reporting chicken
or poultry-related environments as the main source of human campylobacteriosis
infections. Most clonal complexes (CCs) associated with chicken host specialization
(e.g., ST-354, ST-573, ST-464, and ST-446) were the CCs with the highest prevalence
of determinants of resistance to Be, Qu, and Te. Finally, a clear trend toward an increase
in the occurrence of Te and Qu resistance determinants on C. jejuni, linked to the spread
of the co-occurrence of the bla OXA−61 and tet(O)-tet(O/W/O) genes and the gyrA (T86I)
SNP, was found from 2001 to date in Europe.S
Application of lactic acid bacteria for the biopreservation of meat products: A systematic review
Systematic ReviewThe increasing concern of consumers about food quality and safety and their rejection of chemical additives has
promoted the breakthrough of the biopreservation field and the development of studies on the use of beneficial
bacteria and their metabolites as potential natural antimicrobials for shelf life extension and enhanced food
safety. Control of foodborne pathogens in meat and meat products represents a serious challenge for the food
industry which can be addressed through the intelligent use of bio-compounds or biopreservatives. This article
aims to systematically review the available knowledge about biological strategies based on the use of lactic acid
bacteria to control the proliferation of undesirable microorganisms in different meat products. The outcome of
the literature search evidenced the potential of several strains of lactic acid bacteria and their purified or semi-
purified antimicrobial metabolites as biopreservatives in meat products for achieving longer shelf life or
inhibiting spoilage and pathogenic bacteria, especially when combined with other technologies to achieve a
synergistic effect.S
Application of lactic acid bacteria for the biopreservation of meat products: A systematic review
.The increasing concern of consumers about food quality and safety and their rejection of chemical additives has promoted the breakthrough of the biopreservation field and the development of studies on the use of beneficial bacteria and their metabolites as potential natural antimicrobials for shelf life extension and enhanced food safety. Control of foodborne pathogens in meat and meat products represents a serious challenge for the food industry which can be addressed through the intelligent use of bio-compounds or biopreservatives. This article aims to systematically review the available knowledge about biological strategies based on the use of lactic acid bacteria to control the proliferation of undesirable microorganisms in different meat products. The outcome of the literature search evidenced the potential of several strains of lactic acid bacteria and their purified or semi-purified antimicrobial metabolites as biopreservatives in meat products for achieving longer shelf life or inhibiting spoilage and pathogenic bacteria, especially when combined with other technologies to achieve a synergistic effect.S
Microbiological Safety and Shelf-Life of Low-Salt Meat Products—A Review
[EN] Salt is widely employed in different foods, especially in meat products, due to its very diverse and extended functionality. However, the high intake of sodium chloride in human diet has been under consideration for the last years, because it is related to serious health problems. The meat-processing industry and research institutions are evaluating different strategies to overcome the elevated salt concentrations in products without a quality reduction. Several properties could be directly or indirectly affected by a sodium chloride decrease. Among them, microbial stability could be shifted towards pathogen growth, posing a serious public health threat. Nonetheless, the majority of the literature available focuses attention on the sensorial and technological challenges that salt reduction implies. Thereafter, the need to discuss the consequences for shelf-life and microbial safety should be considered. Hence, this review aims to merge all the available knowledge regarding salt reduction in meat products, providing an assessment on how to obtain low salt products that are sensorily accepted by the consumer, technologically feasible from the perspective of the industry, and, in particular, safe with respect to microbial stability.S
A European questionnaire-based study on population awareness and risk perception of antimicrobial resistance
Versión post-printTo tackle antimicrobial resistance (AMR) is of outmost importance for the general population to understand the severity and the relevance of different routes of transmission. Respondents of different age groups, educational and occupational backgrounds, area of living, diet and household composition participated in an online survey with questions concerning socio-demographics, personal use of antibiotics, awareness, general knowledge, sources of information, behavior and attitude toward antibiotics, and risk perception on antibiotics and AMR. Descriptive and logistic regression analyses were carried out. A total of 1252 respondents, mainly from EU, participated in the survey. About 57.7% declared they consumed antibiotics in the last year and some misguided behaviors were identified, especially for those not having a food- or health-related background, who more frequently failed in giving the right answer to uncontroversial true/false questions (ANOVA, P < 0.05). The youngest respondents were less confident on the information received from traditional media (OR = 0.425), the national government (OR = 0.462), and consumer organizations (OR = 0.497), while they frequently obtained information from social networks and online media, which could therefore be exploited as a channel for educational campaigns targeting this population group. New measures, strategies and policy agenda at a European level aimed at improving awareness on AMR among targeted community groups must be taken into consideration.S
Plasma-activated water: A cutting-edge technology driving innovation in the food industry
.Innovation regarding food production and processing is required to meet the emerging challenges of ensuring worldwide food security and meeting consumer demands for high-quality, safe and nutritious food products. This review provides insights into the current state-of-the-art of the emerging applications of Plasma Activated Water (PAW) in the food industry. PAW antimicrobial properties, inactivation mechanisms and the critical factors determining the lethal effect, as well as the bases for other technological applications are discussed. Overall, this review article describes the degree of success achieved by PAW technology in different applications and illustrates its feasibility and applicability in the food-processing industryS
Evidence of virulence and antibiotic resistance genes from the microbiome mapping in minimally processed vegetables producing facilities
Daily consumption of fresh vegetables is highly recommended by international health organizations, because of their high content of nutrients. However, fresh vegetables might harbour several pathogenic microorganisms or contribute to spread antibiotic resistance, thus representing a hazard for consumers. In addition, little is known about the transmission routes of the residential microbiome from the food handling environment to vegetables. Therefore, we collected environmental and food samples from three manufactures producing fresh vegetables to estimate the relevance of the built environment microbiome on that of the finished products. Our results show that food contact surfaces sampled after routine cleaning and disinfection procedures host a highly diverse microbiome, including pathogens such as the enterotoxigenic Bacillus cereus sensu stricto. In addition, we provide evidence of the presence of a wide range of antibiotic resistance and virulence genes on food contact surfaces associated with multiple taxa, thus supporting the hypothesis that selection of resistant and pathogenic taxa might occur on sanitized surfaces. This study also highlights the potential of microbiome mapping routinely applied in food industries monitoring programs to ensure food safety
High pressure processing at the early stages of ripening enhances the safety and quality of dry fermented sausages elaborated with or without starter culture
[EN] To study the quality of chorizo de León dry fermented sausages (DFS), high pressure processing (HPP) applied at the early stages of ripening and the use of a functional starter culture were evaluated as additional safety measures. Furthermore, the ability to control the populations of artificially inoculated Listeria monocytogenes and Salmonella Typhimurium was investigated and the evolution of microbial communities was assessed by amplicon 16S rRNA metataxonomics. The use of HPP and the starter culture, independently or combined, induced a reduction of Listeria monocytogenes of 1.5, 4.3 and > 4.8 log CFU/g respectively, as compared to control. Salmonella Typhimurium counts were under the detection limit (<1 log) in all treated end-product samples. Both additional measures reduced the activity of undesirable microbiota, such as Serratia and Brochothrix, during the production of DFS. Moreover, the starter culture highly influenced the taxonomic profile of samples. No adverse sensory effects were observed, and panelists showed preference for HPP treated DFS. In conclusion, this new approach of applying HPP at the early stages of ripening of DFS in combination with the use of a defined starter culture improved the safety and quality of the meat productS
Microbiota and Cyanotoxin Content of Retail Spirulina Supplements and Spirulina Supplemented Foods
[EN]Cyanobacterial biomass such as spirulina (Arthrospira spp.) is widely available as a food supplement and can also be added to foods as a nutritionally beneficial ingredient. Spirulina is often produced in open ponds, which are vulnerable to contamination by various microorganisms, including some toxin-producing cyanobacteria. This study examined the microbial population of commercially available spirulina products including for the presence of cyanobacterial toxins. Five products (two supplements, three foods) were examined. The microbial populations were determined by culture methods, followed by identification of isolates using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF), and by 16S rRNA amplicon sequencing of the products themselves and of the total growth on the enumeration plates. Toxin analysis was carried out by enzyme-linked immunosorbent assay (ELISA). Several potentially pathogenic bacteria were detected in the products, including Bacillus cereus and Klebsiella pneumoniae. Microcystin toxins were detected in all the products at levels that could lead to consumers exceeding their recommended daily limits. Substantial differences were observed in the identifications obtained using amplicon sequencing and MALDI-TOF, particularly between closely related Bacillus spp. The study showed that there are microbiological safety issues associated with commercial spirulina products that should be addressed, and these are most likely associated with the normal means of production in open ponds.S
- …