615 research outputs found
Comparison of individual and combined effects of salinity and deficit irrigation on physiological, nutritional and ornamental aspects of tolerance in Callistemon laevis plants
The effect of water deficit, salinity and both applied simultaneously on several physiological and morphological parameters in the ornamental plant Callistemon laevis was studied to identify the tolerance mechanisms developed by this species to these sources of stress and to evaluate their adaptability to such conditions. C. laevis plants were grown in pots outdoors and subjected to four irrigation treatments lasting ten months: control (0.8dSm-1, 100% water holding capacity), water deficit (0.8dSm-1, 50% of the amount of water supplied in control), saline (4.0dSm-1, same amount of water supplied as control) and saline water deficit (4.0dSm-1, 50% of the water supplied in the control). Water and saline stress, when applied individually, led to a reduction of 12% and 39% of total biomass, respectively, while overall plant quality (leaf color and flowering) was unaffected. However, saline water deficit affected leaf color and flowering and induced an excessive decrease of growth (68%) due to leaf tissue dehydration and a high leaf Cl and Na concentration. Biomass partitioning depended not only on the amount of water applied, but also on the electrical conductivity of the water. Water stress induced active osmotic adjustment and decreased leaf tissue elasticity. Although both Na and Cl concentrations in the plant tissues increased with salinity, Cl entry through the roots was more restricted. In plants submitted to salinity individually, Na tended to remain in the roots and stems, and little reached the leaves. However, plants simultaneously submitted to water and saline stress were not able to retain this ion in the woody parts. The decrease in stomatal conductance and photosynthesis was more marked in the plants submitted to both stresses, the effect of which decreased photosynthesis, and this together with membrane damage delayed plant recovery. The results show that the combination of deficit irrigation and salinity in C. laevis is not recommended since it magnifies the adverse effects of either when applied individually. © 2015 Elsevier GmbH.This work was supported by the Spanish Ministry of Economy and Competitiveness (project CICYT AGL 2011-30022-C02-01-02) and Fundación Séneca-Agencia de Ciencia y Tecnología de la Región de Murcia (15356/PI/10).Peer Reviewe
Changes in growth rate, root morphology and water use efficiency of potted Callistemon citrinus plants in response to different levels of water deficit
Callistemon is widely used as a flowering shrub in gardening and landscaping in the Mediterranean area. However, prolonged or severe water stress may alter its physiological and morphological behaviour. Callistemon citrinus plants were grown in nursery conditions and subjected to three irrigation treatments: a control (watered to container capacity) and two water deficit treatments of 50 and 25% of the amount of water supplied in the control treatment (moderate and severe deficit irrigation, respectively). After 53 weeks, the moderate deficit irrigation plants showed a lower relative growth rate but increased root/shoot ratio, improved the root system and increased water use efficiency, while flowering and leaf colour were unaffected. However, severe deficit irrigation reduced flowering and affected leaf colour. Both deficit irrigation treatments reduced stomatal conductance, suggesting an efficient and adaptive stomatal control in this species. These reductions were marked after longer periods in plants submitted to severe deficit irrigation, which decreased photosynthesis and could delay plant recovery and cause permanent damage. Differences between stem and leaf water potential values have seen to be a good indicator of instantaneous shoot transpiration. Water consumption was influenced by the active periods of growth and inflorescence formation. It is concluded that moderate deficit irrigation can be used successfully in C. citrinus plant production to reduce water consumption while maintaining good overall quality. © 2013 Elsevier B.V.This work was supported by the Spanish Ministry of Science and Innovation (AGL 2008-05258-C02-1-2, AGL 2011-30022-C02-01) and Fundación Séneca (15356/PI/10).Peer Reviewe
Long-term effect of salinity on plant quality, water relations, photosynthetic parameters and ion distribution in Callistemon citrinus
The effect of saline stress on physiological and morphological parameters in Callistemon citrinus plants was studied to evaluate their adaptability to irrigation with saline water. C. citrinus plants, grown under greenhouse conditions, were subjected to two irrigation treatments lasting 56 weeks: control (0.8 dS·m-1) and saline (4 dS·m-1). The use of saline water in C. citrinus plants decreased aerial growth, increased the root/shoot ratio and improved the root system (increased root diameter and root density), but flowering and leaf colour were not affected. Salinity caused a decrease in stomatal conductance and evapotranspiration, which may prevent toxic levels being reached in the shoot. Net photosynthesis was reduced in plants subjected to salinity, although this response was evident much later than the decrease in stomatal conductance. Stem water potential was a good indicator of salt stress in C. citrinus. The relative salt tolerance of Callistemon was related to storage of higher levels of Na+ and Cl- in the roots compared with the leaves, especially in the case of Na+, which could have helped to maintain the quality of plants. The results show that saline water (around 4 dS·m-1) could be used for growing C. citrinus commercially. However, the cumulative effect of irrigating with saline water for 11 months was a decrease in photosynthesis and intrinsic water use efficiency, meaning that the interaction of the salinity level and the time of exposure to the salt stress should be considered important in this species. © 2014 German Botanical Society and Royal Botanical Society of the Netherlands.This work was supported by the Spanish Ministry of Science and Innovation (AGL 2011-30022-C02-01-02) and Fundación Séneca (15356/PI/10).Peer Reviewe
Regulated deficit irrigation in different phenological stages of potted geranium plants: Water consumption, water relations and ornamental quality
The irrigation water requirements and sensitivity to water deficits of ornamental plants is of great interest to horticultural producers for planning irrigation strategies. The effect of different deficit irrigation strategies on physiological and morphological parameters in geranium plants was studied in different growth phases to evaluate how such strategies can be safely used and to ascertain whether the flowering phase is sensitive to deficit irrigation. Pelargonium × hortorum L. H. Bailey plants, grown in a controlled growth chamber, were subjected to four irrigation treatments: control (100 % water field capacity throughout the experiment), sustainable deficit irrigation (75 % water field capacity throughout the experiment), and two regulated deficit irrigation treatments that included water stress during the vegetative growth phase or during the flowering development phase. Although the total amount of irrigation water was similar in the three deficit irrigation treatments (around 80 % of the control value), the lowest values for both height and flowering were found when deficit irrigation was applied during flowering. This indicates that plant quality does not only depend on the amount of water applied but also on the time when the reduction is applied, and that flowering is the most sensitive phase to water stress. Evapotranspiration was related to the formation of inflorescences and to increased plant height. When the irrigation strategy was changed, plants increased or decreased their water consumption and stomatal conductance to adjust to the new conditions by regulating stomatal opening, although, in general, the values of both parameters remained below those observed in the control plants. © 2012 Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków.This work was supported by the Spanish Ministry of Science and Innovation (AGL 2008-05258-C02-1-2, AGL 2011-30022-C02-01) and Fundación Séneca (15356/PI/10).Peer Reviewe
Regulated deficit irrigation in potted Dianthus plants: Effects of severe and moderate water stress on growth and physiological responses
The purpose of this study was to analyze the physiological and morphological response of carnation plants to different levels of irrigation and to evaluate regulated deficit irrigation as a possible technique for saving water through the application of controlled drought stress. Carnations, Dianthus caryophyllus L. cultivar, were pot-grown in an unheated greenhouse and submitted to two experiments. In the first experiment, the plants were exposed to three irrigation treatments: (control); 70% of the control (moderate deficit irrigation, MDI) and 35% of the control (severe deficit irrigation, SDI). In the second experiment, the plants were submitted to a control treatment, deficit irrigation (DI, 50% of the control) and regulated deficit irrigation (RDI). After 15 weeks, MDI plants showed a slightly reduced total dry weight, plant height and leaf area, while SDI had clearly reduced all the plant size parameters. RDI plants had similar leaf area and total dry weight to the control treatment during the blooming phase. MDI did not affect the number of flowers and no great differences in the colour parameters were observed. RDI plants had higher flower dry weight, while plant quality was affected by the SDI (lower number of shoots and flowers, lower relative chlorophyll content). Leaf osmotic potential decreased with deficit irrigation, but more markedly in SDI, which induced higher values of leaf pressure. Stomatal conductance (gs) decreased in drought conditions more than the photosynthetic rate (Pn). Osmotic adjustment of 0.3 MPa accompanied by decreases in elasticity in response to drought resulted in turgor less at lower leaf water potentials and prevented turgor loss during drought periods. © 2009 Elsevier B.V. All rights reserved.This work was supported by CICYT projects AGL 2005-05588-C02-1 and AGL 2005-05588-C02-2 and by the Consejería de Agricultura y Agua de la Región de Murcia, programme (UPCT-CEBAS-IMIDA.2005).Peer Reviewe
Transpiration, photosynthetic responses, tissue water relations and dry mass partitioning in Callistemon plants during drought conditions
Callistemon is an Australian species used as ornamental plant in Mediterranean regions. The objective of this research was to analyse the ability of Callistemon to overcome water deficit in terms of adjusting its physiology and morphology. Potted Callistemon laevis Anon plants were grown in controlled environment and subjected to drought stress by reducing irrigation water by 40% compared to the control (irrigated to container capacity). The drought stress produced the smallest plants throughout the experiment. After three months of drought, the leaf area, number of leaves and root volume decreased, while root/shoot ratio and root density increased. The higher root hydraulic resistance in stressed plants caused decreases
in leaf and stem water potentials resulting in lower stomatal conductance and indicating that water flow through the roots is a factor that strongly influences shoot water relations. The water stress affected transpiration (63% reduction compared with the control). The consistent decrease in gs suggested an adaptative efficient stomatal control of transpiration by this species, resulting in a higher intrinsic water use efficiency (Pn/gs) in drought conditions, increasing as the experimental time progressed. This was accompanied by an improvement in water use efficiency of production to maintain the leaf water status. In addition, water stress induced an active osmotic adjustment and led to decreases in leaf tissue elasticity in order to maintain turgor. Therefore, the water deficit produced changes in plant water relations, gas exchange and growth in an adaptation process which could promote the faster establishment of this species in gardens or landscaping projects in Mediterranean conditions.This work was supported by the projects: CICYT (AGL 2008-05258-C02-1-2), CDTI (IDI-20070868) and Fundación Seneca (05660/PI/07).Peer Reviewe
Teaching technology: from knowing to feeling enhancing emotional and content acquisition performance through Gardner’s multiple intelligences Theory in technology and design lessons
Gardner’s Multiple Intelligences Theory (MIT) can be a cognitive and emotional improvement if is taken into account in the standard development of the Technology lessons. This work presents a preliminary evaluation of the performance enhancement in two concomitant aspects: contents acquisition and emotional yield. The study was made on up to 150 students with ages of 12-13 years old. The control group was submitted to traditional transmission-reception lessons, whereas the experimental one was submitted to novel educational techniques that included specific activities which took into account the different intelligences styles (IS) inside the classroom. The results clearly depicted that both studied variables underwent a statistical significant enhancement through the application of the MIT-based educational methodPeer Reviewe
Molecular genetics of naringenin biosynthesis, a typical plant secondary metabolite produced by Streptomyces clavuligerus
Background: Some types of flavonoid intermediates seemed to be restricted to plants. Naringenin is a typical plant metabolite, that has never been reported to be produced in prokariotes. Naringenin is formed by the action of a chal cone synthase using as starter 4-coumaroyl-CoA, which in dicotyledonous plants derives from phenylalanine by the action of a phenylalanine ammonia lyase.
Results: A compound produced by Streptomyces clavuligerus has been identified by LC–MS and NMR as naringenin and coelutes in HPLC with a naringenin standard. Genome mining of S. clavuligerus revealed the presence of a gene for a chalcone synthase (ncs), side by side to a gene encoding a P450 cytochrome (ncyP) and separated from a gene encoding a Pal/Tal ammonia lyase (tal). Deletion of any of these genes results in naringenin non producer mutants. Complementation with the deleted gene restores naringenin production in the transformants. Furthermore, narin genin production increases in cultures supplemented with phenylalanine or tyrosine.
Conclusion: This is the first time that naringenin is reported to be produced naturally in a prokariote. Interestingly three non-clustered genes are involved in naringenin production, which is unusual for secondary metabolites. A ten tative pathway for naringenin biosynthesis has been proposed
Growth, water relations and ion accumulation in Phlomis purpurea plants under water deficit and salinity
The effect of different levels of water deficit and salinity on physiological and morphological alterations in Phlomis purpurea plants was studied to evaluate their adaptability to such conditions. P. purpurea plants growing under greenhouse conditions were subjected to four irrigation treatments between November and May 2008: control (C, 1 dS m-1), moderate water deficit (MWD, 1dS m-1, 60% of the control), severe water deficit (SWD, 1 dS m-1, 40% of the control) and saline (S, 4 dS m-1). Aerial dry weight decreased with salinity, response that was more marked in the water deficit treatments, especially SWD. Stem diameter and leaf number were similarly reduced in both water deficit treatments, while leaf area also decreased in saline treated plants. Throughout the experiment, plant height was similar in both control and saline treated plants and was inhibited 10 weeks after application of the deficit irrigation onwards. Only at the end of the experiment were there significant differences in plant height between all treatments. Plants irrigated with saline water had higher Na+ concentrations in their leaves than in their roots and shoots, while Cl- concentrations were similar in leaves and roots, suggesting some resistance to the movement of the latter ions from root to shoots. The accumulation of salt in the leaves was associated with osmotic adjustment, which maintained midday leaf turgor in saline treated plants. However, no osmotic adjustment was observed in plants submitted to water stress. The results indicate that the effect of osmotic stress due to water deficit was more severe than the toxic effect of salt.This work was supported by the projects: CICYT (AGL 2008-05258-C02-1-2),
CDTI (IDI-20070868) and Convenio de la Consejería de Agricultura y Agua de la Región
de Murcia-UPCT-CEBAS, 2008.Peer Reviewe
Differences and similarities between oral and written competence in Spanish pre-university students. A correlation study.
The Foreign Language (FL) section of Spanish University Entrance Examination (EFL-PAU) has had few revisions over the last twenty years. The Spanish government has substituted the old EFL-PAU University Entrance Examination by a high stakes Baccalaureate Final Evaluation. However, further changes are expected in the coming years. Among the most important ones for the Foreign Language Section is a deep and necessary revision with the inclusion of new types of tasks. To try and inform the decisions made for the new Baccalaureate Final Evaluation, this article reports on the quantitative and qualitative analyses conducted thanks to a pilot oral test carried out with 772 recorded candidate performances. Three main goals were established for this research: 1) to find out the most important variables which characterize foreign language learning in secondary school in Spain; 2) to find out if there is any correlation between the oral competence of students at the end of their non-compulsory secondary education (as obtained from the pilot study) and the marks students obtain in the University Entrance Examination (which does not include the oral skill); and 3) to find out any aspects related to EFL teaching and learning which may potentially enhance oral performance. The results of this paper provide rich information on the students? foreign language learning context, the strong correlation between the written and oral competences and the need to pay attention to three variables which foster the development of oral foreign language in secondary school classroomsMinisterio de Ciencia e Innovació
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