鳗鲡的营养需求研究与配合饲料质量评价

鳗鲡(AnguIllASP)是鳗鲡属鱼类的统称,在分类学上隶属于硬骨鱼纲(OSTEICHTHyES)、鳗鲡目(AnguIllI-fOrMES)、鳗鲡科(AnguIllIdAE)、鳗鲡属(AnguIllA),为肉食性鱼类,广泛分布于全球热带、亚热带和温带地区,其公益性行业(农业)科研专项(nyhyzx07-043)部分资

不同镇痛方法对膝关节置换术后疼痛和早期康复的影响

[目的]对比不同镇痛方法对膝关节置换术后疼痛和早期康复的影响。[方法]2016年9月~2017年9月,择期行单侧全膝关节置换术(TKA)的患者75例列入本研究,依据术后镇痛方式,随机均分为3组:连续收肌管阻滞联合关节周围浸润组(CA+P组)、连续股神经阻滞联合关节周围浸润组(CF+P组)、连续硬膜外镇痛组(E组),所有患者均采用腰硬联合麻醉。记录术前、术后静息及活动状态下的疼痛视觉模拟评分(VAS)和股四头肌肌力,记录术后膝关节最大主动屈曲角度(°)和不良反应。[结果]术后24、48、72 h活动状态下,E组患者VAS评分明显高于CA+P组和CF+P组,差异有统计学意义(P<0.05)。CF+P组和E组患者术后6、12、24 h股四头肌肌力下降,差异有统计学意义(P<0.05)。CF+P组和E组术后1、2、3 d膝关节主动屈曲角度明显降低,差异有统计学意义(P<0.05)。E组加用镇痛药物用量及术后并发症的发生率明显高于其他两组,差异有统计学意义(P<0.05)。[结论]连续收肌管阻滞联合关节周围浸润可有效缓解全膝关节置换术患者疼痛,且对股四头肌肌力影响更小,不良反应发生率更低。国家自然科学基金青年项目(项目编号:81501207

Electrocatalytic Oxidation of Ethanol on Pt/nanoTiO_2-CNT Complex Catalysts

通过前驱体Ti(OEt)4直接水解和电化学扫描电沉积法制备在Ti基体上的纳米TiO2-碳纳米管复合膜载Pt(Pt/nanoTiO2-CNT)复合催化剂.透射电镜(TEM)和X射线衍射(XRD)结果表明,锐钛矿型纳米TiO2粒子和Pt纳米粒子(粒径均为5～10nm)均匀地分散在碳纳米管表面.通过循环伏安和计时电流法研究表明,Pt/nanoTiO2-CNT复合催化剂(Pt载量为0.32mg·cm-2)具有高达51.8m2·g-1的电化学活性比表面积,常温常压下对乙醇的电化学氧化具有高催化活性和稳定性,乙醇氧化峰电位分别为0.59、0.96和0.24V,氧化峰电流密度分别达到-115、-113和-75mA·cm-2.复合催化剂对乙醇电氧化的高催化活性可归因于nanoTiO2、CNT和Pt纳米粒子的协同催化作用.Pt/nanoTiO2-CNT complex catalysts were prepared by direct hydrolysis of Ti(OEt)4 and electrochemical scan electrodepositing method. The results of XRD and SEM showed that the nanoTiO2 (anatase) and Pt nanoparticles (size about 5～10 nm) were dispersed uniformly on CNT film surface. The electrocatalytic activity of Pt/nanoTiO2-CNT electrode was investigated by cyclic voltammetry and chronopotentiogram. The results indicated that Pt/nanoTiO2-CNT electrode (Pt loading was 0.32 mg·cm-2) exhibited high electrochemical activity surface area (51.8 m2·g-1) and very high electrocatalytic activity and stability for the electro-oxidation of ethanol at room temperature in atmosphere pressure. The oxidation peak potentials of ethanol were 0.59, 0.96, and 0.24 V, and the corresponding oxidation peak current sdensities were -115, -113, and -75 mA·cm-2. The high electrocatalytic activity and good stability can be attributed to the synergistic catalytic effect among nanocomposite.国家自然科学基金(20476001);; 安徽省自然科学基金(00045317)资助项

Effects of Heavy Metal and Pollutants on the Non-special Immunity of the Shrimp and Crab

综述了近10a国内外有关重金属、氨态氮、亚硝酸盐、有机污染物等几种环境因子对虾蟹类非特异性免疫影响的研究成果,以期为通过改善养殖环境条件提高虾蟹类自身免疫抗病力提供理论依据。同时,为进一步深入开展该领域的研究工作积累资料。This paper attempted to review and evaluate existing information about the effects of heavy metal,ammonia-N,nitrite and the organic pollutants on the non-special immune response of the shrimp and crab,which providing theory for improving the self-immunity of shrimp and crab by meliorating cultural environment. In addition,it could provide information for further study on this field

Comparison of methods for determination of immunogenicity of recombinant human papillomavirus vaccine

目的评价重组人乳头瘤病毒(HuMAn PAPIllOMAVIruS,HPV)疫苗免疫原性2种检测方法的相关性。方法应用间接酶联免疫吸附(ElISA)法和假病毒中和(PSEudOVIrIOn-bASEd nEuTrAlIzATIOn ASSAy,PbnS)法对26份阴性血清样本、34份自然感染HPV16或HPV18的血清样本及30份接种HPV16/18型双价疫苗后7个月的血清样本进行抗体滴度检测,并采用PEArMAn进行相关性分析。结果 3组样本的抗HPV16型和HPV18型抗体滴度的2次检测结果比值显示,试验室内重复性较好;2种方法检测血清样本抗HPV16型抗体滴度的总体相关系数为0.826,抗HPV18型为0.921;自然感染HPV的血清样本抗HPV16型抗体滴度的相关系数为0.519,抗HPV18型为0.613;接种HPV16/18型双价疫苗后的血清样本抗HPV16型抗体滴度的相关系数为0.671,抗HPV18型为0.879。结论间接ElISA法和PbnS法在检测血清样本中抗HPV16/18型抗体滴度时均具有较好的重复性和相关性,其中以接种疫苗后的血清样本中抗体滴度相关性最佳,为HPV疫苗免疫原性的检测及剂量探索试验提供了参考依据。Objective To assess the correlation between two methods for determination of immunogenicity of recombinant human papillomavirus(HPV) vaccine.Methods Enzyme-linked immunosorbent assay(ELISA) and pseudovirion-based neutralization assay(PBNS) were used to measure the anti-HPV 16 or anti-HPV 18 antibody levels in 26 negative serum samples,34 serum samples naturally infected with HPV16 or HPV18 and 30 serum samples 7 months after immunization with HPV16 / 18 bivalent vaccine,between which the correlation was analyzed by Pearman method.Results The ratio of results of two tests for HPV16 and HPV18 antibody titers in three groups of samples showed high intra-reproducibility.The total correlation coefficient of test results of HPV16 antibody titers by two methods was 0.826,while that of HPV18 antibody was 0.921.The correlation coefficient for HPV 16 antibody titer in naturally infected samples was 0.519,while that for HPV18 antibody titer was 0.613.However,the correlation coefficient for HPV 16 antibody titer in serum samples after immunization with HPV16 / 18 bivalent vaccine was 0.671,while that for HPV 18 antibody titer was 0.879.Conclusion Both ELISA and PBNS showed high reproducibility and correlation for determination of HPV16 / 18 antibody titers in serum samples,especially for those in samples after vaccination.It provided a reference for determination of immunogenicity and optimization of dosage of HPV vaccine.国家高技术研究发展计划(2012AA02A402); Themajorpro-jectofInternationalScienceandTechnologyCollaborativeProgram(2010DFB30100

Electrocatalytic oxidation of ethanol on Pt/nanoTiO(2)-CNT complex catalysts

Pt/nanoTiO(2)-CNT complex catalysts were prepared by direct hydrolysis of Ti(OEt)(4) and electrochemical scan electrodepositing method. The results of XRD and SEM showed that the nanoTiO(2) (anatase) and Pt nanoparticles (size about 5 similar to 10 nm) were dispersed uniformly on CNT film surface. The electrocatalytic activity of Pt/nanoTiO(2)-CNT electrode was investigated by cyclic voltammetry and chronopotentiogram. The results indicated that Pt/nanoTiO(2)- CNT electrode (Pt loading was 0.32 mg . cm(-2)) exhibited high electrochemical activity surface area (51.8 m2. g(-1)) and very high electrocatalytic activity and stability for the electro-oxidation of ethanol at room temperature in atmosphere pressure. The oxidation peak potentials of ethanol were 0.59, 0.96, and 0.24 V, and the corresponding oxidation peak current sdensities were - 115, -113, and -75 mA . cm(-2). The high electrocatalytic activity and good stability can be attributed to the synergistic catalytic effect among nanocomposite