Kualitas dan Kuantitas Sperma Kambing Bligon Jantan yang Diberi Pakan Rumput Gajah dengan Suplementasi Tepung Darah (Quality and Quantity of Semen of Buck Bligon Fed Elephant Grass Supplemented with Blood Meal).

The experiment was conducted to determine the quality and quantity of semen of buck Bligon fed elephant grass supplemented with blood meal. The experiment was carried out in Laboratory of Animal Physiology and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta. Nine bucks Bligon age 8 to 12 months were used in the experiment. The experimental animals were devided into three groups of feed treatment, i.e group 1 (R0); elephant grass (60%) + corn meal (15%) + rice bran (15%) + soybean cake (10%), group 2 (R1); elephant grass (60%) + corn meal (15%) + rice bran (15%) + soybean cake (5%) + blood meal (5%), group 3 (R2); elephant grass (60%) + corn meal (15%) + rice bran (15%) + blood meal (10%). The experiment was carried out for 3 months. Semen collection using artificial vagina was done two times a week for four weeks. The variables measured were semen volume, semen pH, sperm motility, sperm consentration, sperm viability, and sperm abnormality. The experiment data were statisticaly analyzed using completely randomized design using statistical analysis and then continued with Duncan test to analyze the differences between means. The results showed that the suplementation of blood meal significantly increased (P≤0.01) semen volume. Semen volume for R0 was 0.42 ml lower than R1 0.82 ml and R2 0.69 ml whereas R1 and R2 differed significantly (P≤0.05). The treatments have significant effect (P≤0.01) on sperm motility. The highest sperm motility value was at treatment R1 which was 84.17% followed by R2 81.67% and the lowest was R0 65.00%. The effect of treatment on sperm concentration also significant (P≤0.01). Sperm concentration of R1 was 5,537.67 million cell/ml semen and R2 was 4,415.33 million cell/ml semen which were higher than R0 (3,081.00 million cell/ml semen). Viability was significantly affected (P≤0.01) by the treatments. Viability value at treatment R0 was 69.88% and for treatment R1 and R2 were 91.25% and 87.63% respectively, the values differed significantly (P≤0.05). The effect of treatment on pH semen and sperm abnormality was not significant. Feed containing blood meal, corn meal, rice bran, and soybean could increase the quality and quantity buck Bligon semen. The improvement of local goat livestock productivity could be done by giving blood meal as an alternative of protein source for feed supplement, particularly at areas having low quality feed. (Key words : Goat, Semen quality, Blood meal

MODIFIKASI PENETASAN TELUR BURUNG MALEO GUNUNG (Aepypodius arfakianus) UNTUK MENINGKATKAN DAYA TETAS

his research aims to determine the physical characteristics and quality of eggs of mountain Maleo bird (Aepypodius arfakianus) in Papua and to determine hatching rate by using some modified hatching method to improvethe egg’s hatchability. This research used observation method to analyze variable egg hatching, temperature and humidity. There were three experiments of hatching method, which were semi-natural hatching outside of the habitat(ex-situ), hatching by using soil and serasah as the media, and hatching by using electric shelves as the media. Observation data of natural hatching showed that the temperature inside of the nest was about 30-35oC with humidityof 70-90%. The variables measured were embryo durability or fertility, hatchability, period of incubation, hatch weight, and embryo mortality. The data were analyzed using descriptive statistics and correlations for some data required and was presented as mean values and standard deviation (SD). Hatching using electric shelves as the media showed the best result. Length of incubation, egg fertility, hatchability, embryo mortality and hatch weight were 40.14±0.69 days,90.00%, 77.78%, 22.22%, 112.94±7.84 g, respectively; those of ex-situ modification were 47.50±0.71 days, 80%, 25%, 75%, 109.05±2.33 g, respectively; and those of soil and serasah media were 41.33±0.58 days, 90%, 33.33%, 66.67%, 113.33±5.05 g, respectively. Hatching modifications using electric shelves improves the quality of hatching, shorten theperiod of incubation, and reduced the mortality.(Keywords: Mountain Maleo (Aepypodius arfakianus), Hatching modification, Hatchability

Sikap Mengutamakan Keselamatan Kerja: Tinjauan Faktor Pelatihan Dan Ukuran Pekerja Industri

The study seeks to find out whether safety-training has the same effect in small-scale, middle-scale and large-scale industries. The population is low level education workers in East Java industries. The research found that safety-training affects significantly in increasing workers attitude only in large scale industries. Workers in small and middle industries got safety knowledge from safety-training but their safety attitudes do not increase because they found threat to apply their knowledge in their industries

Kualitas dan Kuantitas Sperma Kambing Bligon Jantan yang Diberi Pakan Rumput Gajah dengan Suplementasi Tepung Darah (Quality and Quantity of Semen of Buck Bligon Fed Elephant Grass Supplemented with Blood Meal).

The experiment was conducted to determine the quality and quantity of semen of buck Bligon fed elephant grass supplemented with blood meal. The experiment was carried out in Laboratory of Animal Physiology and Reproduction,Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta. Nine bucks Bligon age 8 to 12 months were used in the experiment. The experimental animals were devided into three groups of feed treatment, i.e group 1 (R0); elephant grass (60%) + corn meal (15%) + rice bran (15%) + soybean cake (10%), group 2 (R1); elephant grass (60%) + corn meal (15%) + rice bran (15%) + soybean cake (5%) + blood meal (5%), group 3 (R2); elephant grass (60%) + corn meal (15%) + rice bran (15%) + blood meal (10%). The experiment was carried out for 3 months. Semen collection using artificial vagina was done two times a week for four weeks. The variables measured were semen volume, semen pH, sperm motility, sperm consentration, sperm viability, and sperm abnormality. The experiment data were statisticaly analyzed using completely randomized design using statistical analysis and then continued with Duncan test to analyze the differences between means. The results showed that the suplementation of blood meal significantly increased (P≤0.01) semen volume. Semen volume for R0 was 0.42 ml lower than R1 0.82 ml and R2 0.69 ml whereas R1 and R2 differed significantly (P≤0.05). The treatments have significant effect (P≤0.01) on sperm motility. The highest spermmotility value was at treatment R1 which was 84.17% followed by R2 81.67% and the lowest was R0 65.00%. The effect of treatment on sperm concentration also significant (P≤0.01). Sperm concentration of R1 was 5,537.67 million cell/mlsemen and R2 was 4,415.33 million cell/ml semen which were higher than R0 (3,081.00 million cell/ml semen). Viability was significantly affected (P≤0.01) by the treatments. Viability value at treatment R0 was 69.88% and for treatment R1 and R2 were 91.25% and 87.63% respectively, the values differed significantly (P≤0.05). The effect of treatment on pH semen and sperm abnormality was not significant. Feed containing blood meal, corn meal, rice bran, and soybeancould increase the quality and quantity buck Bligon semen. The improvement of local goat livestock productivity could be done by giving blood meal as an alternative of protein source for feed supplement, particularly at areas having lowquality feed.(Key words : Goat, Semen quality, Blood meal

Suplementasi Hormon Gonadotropin Pada Medium Maturasi In Vitro Untuk Meningkatkan Perkembangan Embrio Stadium 4 Sel Kambing Bligon

The study was carried out to investigate the effect of gonadotropin hormone supplementation into in vitro maturation medium on maturation, fertilization and embryo development of Bligon goats. This research steps consist of oocyte collection, in vitro maturation, in vitro fertilization, and in vitro embryo development. At the maturation stage the oocyte that had been collected and divided into two groups based on the maturation medium, that was tissue culture medium (TCM) with supplementation of GnRH 0 IU/mL and GnRH 25 IU/mL. Oocyte and embryo morphology data were analyzed descriptively. Maturation rate and embryo development data were analyzed by using independent sample t-test. Fertilization data was analyzed descriptively. The result showed the percentages of mature oocytes from gonadotropin supplementation of 0 IU/mL and 25 IU/mL were 54.10±25.97 and 54.89±26.44%, respectively. Expansion cumulus cells surrounding the oocytes might indicated the mature oocytes. Cleavage rate of the 2 cells stage were 13,02±11,09 and 27,01±16,65%; respectively, and for the 4 cells stage were 10,16±10,01% and 16,67±14.91%. Embryos obtained from the treatment, indicated uniform of blastomeres in the size, tight, compact, intact, and round-spherical shape. It could be concluded that supplementation of gonadotropin hormone into in vitro maturation medium could not increase the rate of oocyte maturation and 4 cell embryo development, but it could increase 2 cell embryo development of Bligon goats. Hormone supplementation could improved the maturation and embryo quality

Pengaruh Ovariektomi terhadap Abortus dan Populasi Sel Trophoblast Serta Sel Mikrofag pada Mencit Bunting

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In-Sacco Degradability of Various Legume-Tree Leaves from Limestone and Non-Limestone Areas in Malang

ABSTRACT: This research alined to determine the dry matter (DM), organic matter (OM) and crude protein (CP) degradation in sacco of various legume tree leaves from limestone and non - limestone areas in Malang. Five samples of legume tree leaves namely leucacna, calliandra, giiriciciia, sesbania and erytheina were incubated at 0,4,8.12,16,24, and 48 hours in the rumen of three fistulated fat-tail sheep (t28,4 kg live weight, the pH of the rumen fluid 6,8 and N113 3,43 niM), Those fistulatcd animals were fed the ammoniated rice straw freely (CP 11,7%, DM intake 633,1 g) and susu PAP concentrate (CP 18,3% , DM intake 26)1,0 g). The formula that has been used to calculate the DM, OM and CI\u27 degradability at the timc "t" is expressed asap = a + b (I - cc() for t_0, the similar modified formula has been used this experiment expressed that(a\u27 + b\u27) = the potential degradability of the feed, a\u27 constant = the feeds that loss during washingb\u27 constant (a+b) a\u27c\u27 constant the degradation rate. The result of this experiment showed that the locational factor gave a bigly significant (

THE EFFECT OF SEMEN WASHING AND SOYBEAN LECITHIN LEVEL ON MOTILITY AND VIABILITY OF RAM SPERMATOZOA STORED AT 5oC

The objectives of this reserarch were to study the effect of semen washing and soybean lecithin level on the motility and viability of ram spermatozoa stored at 5oC. Semen was collected from three mature rams by artificial vagina. Semen was evaluated by macroscopic and microscopic. Semen was divided into ten tubes, the semen of first five tubes was unwashed (W0) and diluted with extender 0%, 1%, 2%, 3% and 4% soybean lecithin (L0, L1, L2, L3 and L4). Semen of another five tubes were washed (W1) then each diluted with similar extender levels. The diluted semen samples were stored at 5oC and the sperm motility and viability were evaluated each day. The interaction of the semen washing and soybean lecithin levels were no significant differences (P>0.05) but the single factor of soybean lecithin level was significant differeNces (P<0.05) and the single factor of semen washing were no significant differences (P>0.05) on progressive motility and viability of ram sperm.The best extender was 3% soybean lecithin (L3) with the percentage of ram sperm progressive motility was 63.18 ± 3.61% and viability was 71.76±2.32%