299 research outputs found
Highly Scalable Algorithms for Robust String Barcoding
String barcoding is a recently introduced technique for genomic-based
identification of microorganisms. In this paper we describe the engineering of
highly scalable algorithms for robust string barcoding. Our methods enable
distinguisher selection based on whole genomic sequences of hundreds of
microorganisms of up to bacterial size on a well-equipped workstation, and can
be easily parallelized to further extend the applicability range to thousands
of bacterial size genomes. Experimental results on both randomly generated and
NCBI genomic data show that whole-genome based selection results in a number of
distinguishers nearly matching the information theoretic lower bounds for the
problem
The approximability of the String Barcoding problem
The String Barcoding (SBC) problem, introduced by Rash and Gusfield (RECOMB, 2002), consists in finding a minimum set of substrings that can be used to distinguish between all members of a set of given strings. In a computational biology context, the given strings represent a set of known viruses, while the substrings can be used as probes for an hybridization experiment via microarray. Eventually, one aims at the classification of new strings (unknown viruses) through the result of the hybridization experiment. In this paper we show that SBC is as hard to approximate as Set Cover. Furthermore, we show that the constrained version of SBC (with probes of bounded length) is also hard to approximate. These negative results are tight
High-Throughput SNP Genotyping by SBE/SBH
Despite much progress over the past decade, current Single Nucleotide
Polymorphism (SNP) genotyping technologies still offer an insufficient degree
of multiplexing when required to handle user-selected sets of SNPs. In this
paper we propose a new genotyping assay architecture combining multiplexed
solution-phase single-base extension (SBE) reactions with sequencing by
hybridization (SBH) using universal DNA arrays such as all -mer arrays. In
addition to PCR amplification of genomic DNA, SNP genotyping using SBE/SBH
assays involves the following steps: (1) Synthesizing primers complementing the
genomic sequence immediately preceding SNPs of interest; (2) Hybridizing these
primers with the genomic DNA; (3) Extending each primer by a single base using
polymerase enzyme and dideoxynucleotides labeled with 4 different fluorescent
dyes; and finally (4) Hybridizing extended primers to a universal DNA array and
determining the identity of the bases that extend each primer by hybridization
pattern analysis. Our contributions include a study of multiplexing algorithms
for SBE/SBH genotyping assays and preliminary experimental results showing the
achievable tradeoffs between the number of array probes and primer length on
one hand and the number of SNPs that can be assayed simultaneously on the
other. Simulation results on datasets both randomly generated and extracted
from the NCBI dbSNP database suggest that the SBE/SBH architecture provides a
flexible and cost-effective alternative to genotyping assays currently used in
the industry, enabling genotyping of up to hundreds of thousands of
user-specified SNPs per assay.Comment: 19 page
The string barcoding problem
In this paper we consider an approach to solve the string barcoding problem. this approach is based on an explicit reduction from the problem to the satisfiability problem
The shortest common superstring problem
We consider the problem of the shortest common superstring. We describe an approach to solve the problem. This approach is based on an explicit reduction from the problem to the satisfiability problem. © 2013 Anna Gorbenko and Vladimir Popov
Restricted common superstrings
In this paper we consider an approach to solve the restricted common superstring problem. This approach is based on an explicit reduction from the problem to the satisfiability problem. © 2013 Anna Gorbenko and Vladimir Popov
The minimum test collection problem
In this paper we consider an approach to solve the minimum test collection problem. This approach is based on an explicit reduction from the problem to the satisfiability problem
The shortest common parameterized supersequence problem
In this paper, we consider the problem of the shortest common parameterized supersequence. In particular, we consider an explicit reduction from the problem to the satisfiability problem. © 2013 Anna Gorbenko and Vladimir Popov
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