Altered mitochondrial function and energy metabolism is associated with a radioresistant phenotype in oesophageal adenocarcinoma

Neoadjuvant chemoradiation therapy (CRT) is increasingly the standard of care for locally advanced oesophageal cancer. A complete pathological response to CRT is associated with a favourable outcome. Radiation therapy is important for local tumour control, however, radioresistance remains a substantial clinical problem. We hypothesise that alterations in mitochondrial function and energy metabolism are involved in the radioresistance of oesophageal adenocarcinoma (OAC). To investigate this, we used an established isogenic cell line model of radioresistant OAC. Radioresistant cells (OE33 R) demonstrated significantly increased levels of random mitochondrial mutations, which were coupled with alterations in mitochondrial function, size, morphology and gene expression, supporting a role for mitochondrial dysfunction in the radioresistance of this model. OE33 R cells also demonstrated altered bioenergetics, demonstrating significantly increased intracellular ATP levels, which was attributed to enhanced mitochondrial respiration. Radioresistant cells also demonstrated metabolic plasticity, efficiently switching between the glycolysis and oxidative phosphorylation energy metabolism pathways, which were accompanied by enhanced clonogenic survival. This data was supported in vivo, in pre-treatment OAC tumour tissue. Tumour ATP5B expression, a marker of oxidative phosphorylation, was significantly increased in patients who subsequently had a poor pathological response to neoadjuvant CRT. This suggests for the first time, a role for specific mitochondrial alterations and metabolic remodelling in the radioresistance of OAC

Impact of services liberalization on productivity of manufacturing firms: evidence from Ukrainian firm-level data

This paper brings new evidence on the impact of services liberalization on performance of manufacturing firms. Using a unique database of Ukrainian firms in 2001-2007, we utilize an external push for liberalization in services sector as a source of exogenous variation to identify the impact of services liberalization on total factor productivity (TFP) of manufacturing firms. Results indicate that a standard deviation increase in services liberalization is associated with a 9 percent increase in TFP. Allowing services liberalization to dynamically influence TFP through the investment channel leads to even higher effect. The effect is robust to different estimation methods and to different sub-samples of the data. In particular, it is more pronounced for domestic and small firms.services liberalization, productivity, trade

Services liberalization and productivity of manufacturing firms : evidence from Ukraine

This paper brings new evidence on the impact of services liberalization on the performance of manufacturing firms. Using a unique database of Ukrainian firms in 2001-2007, the authors utilize an external push for liberalization in the services sector as a source of exogenous variation to identify the impact of services liberalization on total factor productivity (TFP) of manufacturing firms. The results indicate that a standard deviation increase in services liberalization is associated with a 9 percent increase in TFP. Allowing services liberalization to dynamically influence TFP through the investment channel leads to an even larger effect. The effect is robust to different estimation methods and to different sub-samples of the data. In particular, it is more pronounced for domestic and small firms.Economic Theory&Research,E-Business,Emerging Markets,ICT Policy and Strategies,Public Sector Corruption&Anticorruption Measures

Implication of Additive Manufacturing on United States Air Force Expeditionary Civil Engineer Squadron Supply Chain

Additive manufacturing is mandated as a technology for the Department of Defense to consider to implement. Previous efforts have shown positive potential for additive manufacturing (AM) for United States Air Force Civil Engineering but do not explore the economic impact. This research examines implementation by investigating a specific Explosive Ordnance Disposal repair part supply chain in the current combat theater of operations. A framework to capture the basic financial savings AM could realize was developed to aid AM decision making. This research established a Scenario Planning and Monte Carlo simulation based framework to produce an estimated annual cost for a system with various configurations and machine capabilities under varied machine life lengths. The model informs the baseline value of AM replacement and what this represents for an associated machine cost. Further, the research presents potential roadblocks and additional cost areas that would impact an AM decision. The overall results take the next step to understand AMs implementation for the United States Air Force and Civil Engineer Squadrons

Spartan Daily, November 16, 1959

Volume 47, Issue 38https://scholarworks.sjsu.edu/spartandaily/3954/thumbnail.jp

Playing it safe? a study of the regulation of outdoor play for children and young people in residential care

The aim of the research was to describe and explain the barriers to, and opportunities for, outdoor play and recreation that exist in residential child care services. The research focussed on six residential facilities which represented a cross-section of the sector. Interviews were conducted with two young people, one manager and two basic grade staff members from each of the facilities. The findings were analysed using data display and reduction and the following key findings emerged