Instantaneous baseline damage localisation using sensor mapping

In this paper an instantaneously recorded baseline method is proposed using piezoelectric transducers for damage localisation under varying temperature. This method eliminates need for baselines required when operating at different temper- atures by mapping a baseline area onto the interrogation area. Instantaneously recorded baselines and current interrogation signals are calibrated based on the sensor mapping. This allows extraction of damage scatter signal which is used to localise damage. The proposed method is used to localise actual impact damage on a composite plate under varying temperatures. The method is also applied to a stiffened fuselage panel to accurately localise impact damage

Grating-Coupled Surface Plasmon Resonance (GC-SPR) Optimization for Phase-Interrogation Biosensing in a Microfluidic Chamber.

Surface Plasmon Resonance (SPR)-based sensors have the advantage of being label-free, enzyme-free and real-time. However, their spreading in multidisciplinary research is still mostly limited to prism-coupled devices. Plasmonic gratings, combined with a simple and cost-effective instrumentation, have been poorly developed compared to prism-coupled system mainly due to their lower sensitivity. Here we describe the optimization and signal enhancement of a sensing platform based on phase-interrogation method, which entails the exploitation of a nanostructured sensor. This technique is particularly suitable for integration of the plasmonic sensor in a lab-on-a-chip platform and can be used in a microfluidic chamber to ease the sensing procedures and limit the injected volume. The careful optimization of most suitable experimental parameters by numerical simulations leads to a 30–50% enhancement of SPR response, opening new possibilities for applications in the biomedical research field while maintaining the ease and versatility of the configuration

Fast reliable interrogation of procedurally defined implicit surfaces using extended revised affine arithmetic.

Techniques based on interval and previous termaffine arithmetic next term and their modifications are shown to provide previous term reliable next term function range evaluation for the purposes of previous termsurface interrogation.next term In this paper we present a technique for the previous termreliable interrogation of implicit surfacesnext term using a modification of previous termaffine arithmeticnext term called previous term revised affine arithmetic.next term We extend the range of functions presented in previous termrevised affine arithmeticnext term by introducing previous termaffinenext term operations for arbitrary functions such as set-theoretic operations with R-functions, blending and conditional operators. The obtained previous termaffinenext term forms of arbitrary functions provide previous termfasternext term and tighter function range evaluation. Several case studies for operations using previous termaffinenext term forms are presented. The proposed techniques for previous termsurface interrogationnext term are tested using ray-previous termsurfacenext term intersection for ray-tracing and spatial cell enumeration for polygonisation. These applications with our extensions provide previous termfast and reliablenext term rendering of a wide range of arbitrary previous termprocedurally defined implicit surfacesnext term (including polynomial previous termsurfaces,next term constructive solids, pseudo-random objects, previous termprocedurally definednext term microstructures, and others). We compare the function range evaluation technique based on previous termextended revised affine arithmeticnext term with other previous termreliablenext term techniques based on interval and previous termaffine arithmeticnext term to show that our technique provides the previous termfastestnext term and tightest function range evaluation for previous termfast and reliable interrogation of procedurally defined implicit surfaces.next term Research Highlights The main contributions of this paper are as follows. ► The widening of the scope of previous termreliablenext term ray-tracing and spatial enumeration algorithms for previous termsurfacesnext term ranging from algebraic previous termsurfaces (definednext term by polynomials) to general previous termimplicit surfaces (definednext term by function evaluation procedures involving both previous termaffinenext term and non-previous termaffinenext term operations based on previous termrevised affine arithmetic)next term. ► The introduction of a technique for representing procedural models using special previous termaffinenext term forms (illustrated by case studies of previous termaffinenext term forms for set-theoretic operations in the form of R-functions, blending operations and conditional operations). ► The detailed derivation of special previous termaffinenext term forms for arbitrary operators

Labeless and reversible immunosensor assay based upon an electrochemical current-transient protocol

A novel labeless and reversible immunoassay based upon an electrochemical current-transient protocol is reported which offers many advantages in comparison to classical immuno-biochemical analyses in terms of simplicity, speed of response, reusability and possibility of multiple determinations. Conducting polypyrrole films containing antibodies against 1) Bovine Serum Albumin (BSA) and 2) Digoxin were deposited on the surface of platinum electrodes to produce conductive affinity matrices having clearly defined binding characteristics. The deposition process has been investigated using 125I labelled anti-digoxin to determine optimal fabrication protocols. Antibody integrity and activity, together with non-specific binding of antigen on the conducting matrix have also been investigated using tritiated digoxin to probe polypyrrole/anti-digoxin films. Amperometric responses to digoxin were recorded in flow conditions using these films, but the technique was limited in use mainly due to baseline instability. Anti-BSA - polypyrrole matrices were investigated in more detail in both flow and quiescent conditions. No observable response was found in flow conditions, however under quiescent conditions (in non-stirred batch cell), anti-BSA – polypyrrole films have been demonstrated to function as novel quantitative chronoamperometric immuno-biosensors when interrogated using a pulsed potential waveform. The behaviour of the electrodes showed that the antibody/antigen binding and/or interaction process underlying the response observed was reversible in nature, indicating that the electrodes could be used for multiple sensing protocols. Calibration profiles for BSA demonstrated linearity for a concentration range of 0-50 ppm but tended towards a plateau at higher concentrations. Factors relating to replicate sensor production, sample measurement and reproducibility are discuss