Holographic opto-fluidic microscopy.

Over the last decade microfluidics has created a versatile platform that has significantly advanced the ways in which micro-scale organisms and objects are controlled, processed and investigated, by improving the cost, compactness and throughput aspects of analysis. Microfluidics has also expanded into optics to create reconfigurable and flexible optical devices such as reconfigurable lenses, lasers, waveguides, switches, and on-chip microscopes. Here we present a new opto-fluidic microscopy modality, i.e., Holographic Opto-fluidic Microscopy (HOM), based on lensless holographic imaging. This imaging modality complements the miniaturization provided by microfluidics and would allow the integration of microscopy into existing on-chip microfluidic devices with various functionalities. Our imaging modality utilizes partially coherent in-line holography and pixel super-resolution to create high-resolution amplitude and phase images of the objects flowing within micro-fluidic channels, which we demonstrate by imaging C. elegans, Giardia lamblia, and Mulberry pollen. HOM does not involve complicated fabrication processes or precise alignment, nor does it require a highly uniform flow of objects within microfluidic channels

Lensfree on-chip microscopy over a wide field-of-view using pixel super-resolution.

We demonstrate lensfree holographic microscopy on a chip to achieve approximately 0.6 microm spatial resolution corresponding to a numerical aperture of approximately 0.5 over a large field-of-view of approximately 24 mm2. By using partially coherent illumination from a large aperture (approximately 50 microm), we acquire lower resolution lensfree in-line holograms of the objects with unit fringe magnification. For each lensfree hologram, the pixel size at the sensor chip limits the spatial resolution of the reconstructed image. To circumvent this limitation, we implement a sub-pixel shifting based super-resolution algorithm to effectively recover much higher resolution digital holograms of the objects, permitting sub-micron spatial resolution to be achieved across the entire sensor chip active area, which is also equivalent to the imaging field-of-view (24 mm2) due to unit magnification. We demonstrate the success of this pixel super-resolution approach by imaging patterned transparent substrates, blood smear samples, as well as Caenoharbditis Elegans

Lensfree super-resolution holographic microscopy using wetting films on a chip.

We investigate the use of wetting films to significantly improve the imaging performance of lensfree pixel super-resolution on-chip microscopy, achieving < 1 µm spatial resolution over a large imaging area of ~24 mm(2). Formation of an ultra-thin wetting film over the specimen effectively creates a micro-lens effect over each object, which significantly improves the signal-to-noise-ratio and therefore the resolution of our lensfree images. We validate the performance of this approach through lensfree on-chip imaging of various objects having fine morphological features (with dimensions of e.g., ≤0.5 µm) such as Escherichia coli (E. coli), human sperm, Giardia lamblia trophozoites, polystyrene micro beads as well as red blood cells. These results are especially important for the development of highly sensitive field-portable microscopic analysis tools for resource limited settings

Lensfree computational microscopy tools for cell and tissue imaging at the point-of-care and in low-resource settings.

The recent revolution in digital technologies and information processing methods present important opportunities to transform the way optical imaging is performed, particularly toward improving the throughput of microscopes while at the same time reducing their relative cost and complexity. Lensfree computational microscopy is rapidly emerging toward this end, and by discarding lenses and other bulky optical components of conventional imaging systems, and relying on digital computation instead, it can achieve both reflection and transmission mode microscopy over a large field-of-view within compact, cost-effective and mechanically robust architectures. Such high throughput and miniaturized imaging devices can provide a complementary toolset for telemedicine applications and point-of-care diagnostics by facilitating complex and critical tasks such as cytometry and microscopic analysis of e.g., blood smears, Pap tests and tissue samples. In this article, the basics of these lensfree microscopy modalities will be reviewed, and their clinically relevant applications will be discussed

Superresolution imaging: A survey of current techniques

Cristóbal, G., Gil, E., Šroubek, F., Flusser, J., Miravet, C., Rodríguez, F. B., “Superresolution imaging: A survey of current techniques”, Proceedings of SPIE - The International Society for Optical Engineering, 7074, 2008. Copyright 2008. Society of Photo Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.Imaging plays a key role in many diverse areas of application, such as astronomy, remote sensing, microscopy, and tomography. Owing to imperfections of measuring devices (e.g., optical degradations, limited size of sensors) and instability of the observed scene (e.g., object motion, media turbulence), acquired images can be indistinct, noisy, and may exhibit insufficient spatial and temporal resolution. In particular, several external effects blur images. Techniques for recovering the original image include blind deconvolution (to remove blur) and superresolution (SR). The stability of these methods depends on having more than one image of the same frame. Differences between images are necessary to provide new information, but they can be almost unperceivable. State-of-the-art SR techniques achieve remarkable results in resolution enhancement by estimating the subpixel shifts between images, but they lack any apparatus for calculating the blurs. In this paper, after introducing a review of current SR techniques we describe two recently developed SR methods by the authors. First, we introduce a variational method that minimizes a regularized energy function with respect to the high resolution image and blurs. In this way we establish a unifying way to simultaneously estimate the blurs and the high resolution image. By estimating blurs we automatically estimate shifts with subpixel accuracy, which is inherent for good SR performance. Second, an innovative learning-based algorithm using a neural architecture for SR is described. Comparative experiments on real data illustrate the robustness and utilization of both methods.This research has been partially supported by the following grants: TEC2007-67025/TCM, TEC2006-28009-E, BFI-2003-07276, TIN-2004-04363-C03-03 by the Spanish Ministry of Science and Innovation, and by PROFIT projects FIT-070000-2003-475 and FIT-330100-2004-91. Also, this work has been partially supported by the Czech Ministry of Education under the project No. 1M0572 (Research Center DAR) and by the Czech Science Foundation under the project No. GACR 102/08/1593 and the CSIC-CAS bilateral project 2006CZ002

Bayesian Fusion of Multi-Band Images -Complementary results and supporting materials

Abstract In this paper, a Bayesian fusion technique for remotely sensed multi-band images is presented. The observed images are related to the high spectral and high spatial resolution image to be recovered through physical degradations, e.g., spatial and spectral blurring and/or subsampling defined by the sensor characteristics. The fusion problem is formulated within a Bayesian estimation framework. An appropriate prior distribution exploiting geometrical consideration is introduced. To compute the Bayesian estimator of the scene of interest from its posterior distribution, a Markov chain Monte Carlo algorithm is designed to generate samples asymptotically distributed according to the target distribution. To efficiently sample from this high-dimension distribution, a Hamiltonian Monte Carlo step is introduced in the Gibbs sampling strategy. The efficiency of the proposed fusion method is evaluated with respect to several state-of-the-art fusion techniques. In particular, low spatial resolution hyperspectral and multispectral images are fused to produce a high spatial resolution hyperspectral image. Index Terms Part of this work has been supported by the Hypanema ANR Project

Region-Based Approach for Single Image Super-Resolution

Single image super-resolution (SR) is a technique that generates a high- resolution image from a single low-resolution image [1,2,10,11]. Single image super- resolution can be generally classified into two groups: example-based and self-similarity based SR algorithms. The performance of the example-based SR algorithm depends on the similarity between testing data and the database. Usually, a large database is needed for better performance in general. This would result in heavy computational cost. The self-similarity based SR algorithm can generate a high-resolution (HR) image with sharper edges and fewer ringing artifacts if there is sufficient recurrence within or across scales of the same image [10, 11], but it is hard to generate HR details for an image region with fine texture. Based on the limitation of each type of SR algorithm, we propose to combine these two types of algorithms. We segment each image into regions based on image content, and choose the appropriate SR algorithm to recover the HR image for each region based on the texture feature. Our experimental results show that our proposed method takes advantage of each SR algorithm and can produce natural looking results with sharp edges, while suppressing ringing artifacts. We compute PSNR to qualitatively evaluate the SR results, and our proposed method outperforms the self-similarity based or example-based SR algorithm with higher PSNR (+0.1dB)

Stochastic methods for joint registration, restoration, and interpolation of multiple undersampled images

Abstract—Using a stochastic framework, we propose two algorithms for the problem of obtaining a single high-resolution image from multiple noisy, blurred, and undersampled images. The first is based on a Bayesian formulation that is implemented via the expectation maximization algorithm. The second is based on a maximum a posteriori formulation. In both of our formulations, the registration, noise, and image statistics are treated as unknown parameters. These unknown parameters and the high-resolution image are estimated jointly based on the available observations. We present an efficient implementation of these algorithms in the frequency domain that allows their application to large images. Simulations are presented that test and compare the proposed algorithms