1,018 research outputs found
Large Deformation Diffeomorphic Metric Mapping Registration of Reconstructed 3D Histological Section Images and in vivo MR Images
Our current understanding of neuroanatomical abnormalities in neuropsychiatric diseases is based largely on magnetic resonance imaging (MRI) and post mortem histological analyses of the brain. Further advances in elucidating altered brain structure in these human conditions might emerge from combining MRI and histological methods. We propose a multistage method for registering 3D volumes reconstructed from histological sections to corresponding in vivo MRI volumes from the same subjects: (1) manual segmentation of white matter (WM), gray matter (GM) and cerebrospinal fluid (CSF) compartments in histological sections, (2) alignment of consecutive histological sections using 2D rigid transformation to construct a 3D histological image volume from the aligned sections, (3) registration of reconstructed 3D histological volumes to the corresponding 3D MRI volumes using 3D affine transformation, (4) intensity normalization of images via histogram matching, and (5) registration of the volumes via intensity based large deformation diffeomorphic metric (LDDMM) image matching algorithm. Here we demonstrate the utility of our method in the transfer of cytoarchitectonic information from histological sections to identify regions of interest in MRI scans of nine adult macaque brains for morphometric analyses. LDDMM improved the accuracy of the registration via decreased distances between GM/CSF surfaces after LDDMM (0.39 ± 0.13 mm) compared to distances after affine registration (0.76 ± 0.41 mm). Similarly, WM/GM distances decreased to 0.28 ± 0.16 mm after LDDMM compared to 0.54 ± 0.39 mm after affine registration. The multistage registration method may find broad application for mapping histologically based information, for example, receptor distributions, gene expression, onto MRI volumes
Registration of in-vivo to ex-vivo MRI of surgically resected specimens: A pipeline for histology to in-vivo registration.
BACKGROUND: Advances in MRI have the potential to improve surgical treatment of epilepsy through improved identification and delineation of lesions. However, validation is currently needed to investigate histopathological correlates of these new imaging techniques. The purpose of this work is to develop and evaluate a protocol for deformable image registration of in-vivo to ex-vivo resected brain specimen MRI. This protocol, in conjunction with our previous work on ex-vivo to histology registration, completes a registration pipeline for histology to in-vivo MRI, enabling voxel-based validation of novel and existing MRI techniques with histopathology.
NEW METHOD: A combination of image-based and landmark-based 3D registration was used to register in-vivo MRI and the ex-vivo MRI from patients (N=10) undergoing epilepsy surgery. Target registration error (TRE) was used to assess accuracy and the added benefit of deformable registration.
RESULTS: A mean TRE of 1.35±0.11 and 1.41±0.33mm was found for neocortical and hippocampal specimens respectively. Statistical analysis confirmed that the deformable registration significantly improved the registration accuracy for both specimens.
COMPARISON WITH EXISTING METHODS: Image registration of surgically resected brain specimens is a unique application which presents numerous technical challenges and that have not been fully addressed in previous literature. Our computed TRE are comparable to previous attempts tackling similar applications, as registering in-vivo MRI to whole brain or serial histology.
CONCLUSION: The presented registration pipeline finds dense and accurate spatial correspondence between in-vivo MRI and histology and allows for the spatially local and quantitative assessment of pathological correlates in MRI
Geometry Processing of Conventionally Produced Mouse Brain Slice Images
Brain mapping research in most neuroanatomical laboratories relies on
conventional processing techniques, which often introduce histological
artifacts such as tissue tears and tissue loss. In this paper we present
techniques and algorithms for automatic registration and 3D reconstruction of
conventionally produced mouse brain slices in a standardized atlas space. This
is achieved first by constructing a virtual 3D mouse brain model from annotated
slices of Allen Reference Atlas (ARA). Virtual re-slicing of the reconstructed
model generates ARA-based slice images corresponding to the microscopic images
of histological brain sections. These image pairs are aligned using a geometric
approach through contour images. Histological artifacts in the microscopic
images are detected and removed using Constrained Delaunay Triangulation before
performing global alignment. Finally, non-linear registration is performed by
solving Laplace's equation with Dirichlet boundary conditions. Our methods
provide significant improvements over previously reported registration
techniques for the tested slices in 3D space, especially on slices with
significant histological artifacts. Further, as an application we count the
number of neurons in various anatomical regions using a dataset of 51
microscopic slices from a single mouse brain. This work represents a
significant contribution to this subfield of neuroscience as it provides tools
to neuroanatomist for analyzing and processing histological data.Comment: 14 pages, 11 figure
Current Approaches for Image Fusion of Histological Data with Computed Tomography and Magnetic Resonance Imaging
Classical analysis of biological samples requires the destruction of the tissue’s integrity by cutting or grinding it down to thin slices for (Immuno)-histochemical staining and microscopic analysis. Despite high specificity, encoded in the stained 2D section of the whole tissue, the structural information, especially 3D information, is limited. Computed tomography (CT) or magnetic resonance imaging (MRI) scans performed prior to sectioning in combination with image registration algorithms provide an opportunity to regain access to morphological characteristics as well as to relate histological findings to the 3D structure of the local tissue environment. This review provides a summary of prevalent literature addressing the problem of multimodal coregistration of hard- and soft-tissue in microscopy and tomography. Grouped according to the complexity of the dimensions, including image-to-volume (2D ⟶ 3D), image-to-image (2D ⟶ 2D), and volume-to-volume (3D ⟶ 3D), selected currently applied approaches are investigated by comparing the method accuracy with respect to the limiting resolution of the tomography. Correlation of multimodal imaging could position itself as a useful tool allowing for precise histological diagnostic and allow the a priori planning of tissue extraction like biopsies
Image registration of ex-vivo MRI to sparsely sectioned histology of hippocampal and neocortical temporal lobe specimens.
Intractable or drug-resistant epilepsy occurs in up to 30% of epilepsy patients, with many of these patients undergoing surgical excision of the affected brain region to achieve seizure control. Recent magnetic resonance imaging (MRI) sequences and analysis techniques have the potential to detect abnormalities not identified with diagnostic MRI protocols. Prospective studies involving pre-operative imaging and collection of surgically-resected tissue provide a unique opportunity for verification and tuning of these image analysis techniques, since direct comparison can be made against histopathology, and can lead to better prediction of surgical outcomes and potentially less invasive procedures. To carry out MRI and histology comparison, spatial correspondence between the MR images and the histology images must be found. Towards this goal, a novel pipeline is presented here for bringing ex-vivo MRI of surgically-resected temporal lobe specimens and digital histology into spatial correspondence. The sparsely-sectioned histology images represent a challenge for 3D reconstruction which we address with a combined 3D and 2D registration algorithm that alternates between slice-based and volume-based registration with the ex-vivo MRI. We evaluated our registration method on specimens resected from patients undergoing anterior temporal lobectomy (N=7) and found our method to have a mean target registration error of 0.76±0.66 and 0.98±0.60 mm for hippocampal and neocortical specimens respectively. This work allows for the spatially-local comparison of histology with post-operative MRI and paves the way for eventual correlation with pre-operative MRI image analysis techniques
Joint registration and synthesis using a probabilistic model for alignment of MRI and histological sections
Nonlinear registration of 2D histological sections with corresponding slices
of MRI data is a critical step of 3D histology reconstruction. This task is
difficult due to the large differences in image contrast and resolution, as
well as the complex nonrigid distortions produced when sectioning the sample
and mounting it on the glass slide. It has been shown in brain MRI registration
that better spatial alignment across modalities can be obtained by synthesizing
one modality from the other and then using intra-modality registration metrics,
rather than by using mutual information (MI) as metric. However, such an
approach typically requires a database of aligned images from the two
modalities, which is very difficult to obtain for histology/MRI.
Here, we overcome this limitation with a probabilistic method that
simultaneously solves for registration and synthesis directly on the target
images, without any training data. In our model, the MRI slice is assumed to be
a contrast-warped, spatially deformed version of the histological section. We
use approximate Bayesian inference to iteratively refine the probabilistic
estimate of the synthesis and the registration, while accounting for each
other's uncertainty. Moreover, manually placed landmarks can be seamlessly
integrated in the framework for increased performance.
Experiments on a synthetic dataset show that, compared with MI, the proposed
method makes it possible to use a much more flexible deformation model in the
registration to improve its accuracy, without compromising robustness.
Moreover, our framework also exploits information in manually placed landmarks
more efficiently than MI, since landmarks inform both synthesis and
registration - as opposed to registration alone. Finally, we show qualitative
results on the public Allen atlas, in which the proposed method provides a
clear improvement over MI based registration
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