Demonstrating a multi-primary high dynamic range display system for vision experiments.

We describe the design, construction, calibration, and characterization of a multi-primary high dynamic range (MPHDR) display system for use in vision research. The MPHDR display is the first system to our knowledge to allowfor spatially controllable, high dynamic range stimulus generation using multiple primaries.We demonstrate the high luminance, high dynamic range, and wide color gamut output of the MPHDR display. During characterization, the MPHDR display achieved a maximum luminance of 3200 cd=m2, a maximum contrast range of 3; 240; 000 V 1, and an expanded color gamut tailored to dedicated vision research tasks that spans beyond traditional sRGB displays. We discuss how the MPHDR display could be optimized for psychophysical experiments with photoreceptor isolating stimuli achieved through the method of silent substitution. We present an example case of a range of metameric pairs of melanopsin isolating stimuli across different luminance levels, from an available melanopsin contrast of117%at 75 cd=m2 to a melanopsin contrast of23%at 2000 cd=m2

Using Digital Cameras as Quasi-Spectral Radiometers to Study Complex Fenestration Systems

This work discusses the use of digital cameras fitted with absorption filters as quasi-spectral radiometers. By filtering incident light into selected wavelength intervals, accurate estimates of radiances can be made for unknown spectra. This approach is being employed as part of a new video-projection goniophotometer to study the properties of angularly and spectrally selective complex fenestration systems. Complex fenestration systems are increasingly being used to distribute solar radiation purposefully in buildings. They can be utilized to optimize energy performance and enhance daylighting. Radiance estimates from calibrated digital cameras enable the assessment of quasi-spectral, bi-directional scattering distribution functions of total radiance transmitted or reflected by a fenestration system over desired wavelength intervals. A silicon and an indium gallium arsenide digital camera are used to enable measurements across a 380 to 1700 nm wavelength interval

System of System Integration for Hyperspectral Imaging Microscopy

Hyperspectral imaging (HSI) has become a leading tool in the medical field due to its capabilities for providing assessments of tissue pathology and separation of fluorescence signals. Acquisition speeds have been slow due to the need to acquire signal in many spectral bands and the light losses associated with technologies of spectral filtering. Traditional methods resulted in limited signal strength which placed limitations on time sensitive and photosensitive assays. For example, the distribution of cyclic adenosine monophosphate (cAMP) is largely undetermined because current microscope technologies lack the combination of speed, resolution, and spectral ability to accurately measure Forster resonance energy transfer (FRET). The work presented in this dissertation assesses the feasibility of integrating excitation-scanning hyperspectral imaging methods in widefield and confocal microscopy as a potential solution to improving acquisition speeds without compromising sensitivity and specificity. Our laboratory has previously proposed excitation-scanning approaches to improve signal-to-noise ratio (SNR) and showed that by using excitation-scanning, most-to-all emitted light at each excitation wavelength band can be detected which in turn, increases the SNR. This dissertation describes development and early feasibility studies for two novel prototype concepts as an alternative excitation-scanning HSI technology that may xvi increase acquisition speeds without compromising sensitivity or specificity. To achieve this, two new technologies for excitation-scanning HSI were conceptually designed: - LED-based spectral illumination for widefield microscopy - Supercontinuum-laser-based spectral illumination for spinning disk confocal microscopy. Next, design concepts were theoretically evaluated and optimized, leading to prototype testing. To evaluate the performance of each concept, prototype systems were integrated with other systems and subsystems, calibrated and feasibility assays were executed. This dissertation is divided into three main sections: 1) early development feasibility results of an excitation-scanning widefield system of systems prototype utilizing LED-based HSI, 2) Excitation-scanning HSI and image analysis methods used for endmember identification in fluorescence microscopy studies, and 3) early development feasibility of an excitation-scanning confocal SoS prototype utilizing a supercontinuum laser light source. Integration and testing results proved initial feasibility of both LED-based and broadband-based SoSs. The LED-based light source was successfully tested on a widefield microscope, while the broadband light source system was successfully tested on a confocal microscope. Feasibility for the LED-based system showed that further optical transmission optimization is needed to achieve high acquisition rates without compromising sensitivity or specificity. Early feasibility study results for the broadband-based system showed a successful proof of concept. Findings presented in this dissertation are expected to impact the fields of cellular physiology, medical sciences, and clinical diagnostics by providing the ability for high speed, high sensitivity microscopic imaging with spectroscopic discrimination

Comparison of the accuracy of various transformations from multi-band images to reflectance spectra

This report provides a comparative study of the spectral and colorimetric accuracy of various transformations from multi-band digital signals to spectral reflectance. The multiband channels were obtained by multi-channel visible-spectral imaging (MVSI) using a monochrome CCD and two different filtering systems. In the first system we used a liquid-crystal tunable filter (LCTF) capturing 31 narrow-band channels. We also used a filter wheel with a set of 6 glass filters imaging with and without an extra Wratten absorption filter giving a total of 12 channels. Four different mathematical methods were tested to derive reflectance spectra from digital signals: pseudo-inverse, eigenvector analysis, modified-discrete sine transformation (MDST) and non-negative least squares (NNLS). We also considered two different approaches to sampling the digital signals; in one approach we averaged the digital counts

Standardized spectral and radiometric calibration of consumer cameras

Consumer cameras, particularly onboard smartphones and UAVs, are now commonly used as scientific instruments. However, their data processing pipelines are not optimized for quantitative radiometry and their calibration is more complex than that of scientific cameras. The lack of a standardized calibration methodology limits the interoperability between devices and, in the ever-changing market, ultimately the lifespan of projects using them. We present a standardized methodology and database (SPECTACLE) for spectral and radiometric calibrations of consumer cameras, including linearity, bias variations, read-out noise, dark current, ISO speed and gain, flat-field, and RGB spectral response. This includes golden standard ground-truth methods and do-it-yourself methods suitable for non-experts. Applying this methodology to seven popular cameras, we found high linearity in RAW but not JPEG data, inter-pixel gain variations >400% correlated with large-scale bias and read-out noise patterns, non-trivial ISO speed normalization functions, flat-field correction factors varying by up to 2.79 over the field of view, and both similarities and differences in spectral response. Moreover, these results differed wildly between camera models, highlighting the importance of standardization and a centralized database

New methods for measuring spectral bi-directional transmission and reflection using digital cameras

Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Architecture, 2007.This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.Includes bibliographical references (p. 194-201).Advanced fenestration systems are increasingly being used to distribute solar radiation purposefully in buildings. Distribution of visible light and near infrared radiation can be optimized to enhance daylighting and reduce thermal loads. Light redirecting window systems are one of many innovative fenestration systems available for improving the daylighting and thermal performance of buildings. Many emerging and existing light redirecting systems have both spectrally and angularly selective optical properties. To study these properties, a device that measures the spectral, bi-directional transmission and reflection distribution functions of complex fenestration systems is being developed at the Massachusetts Institute of Technology. This device, a goniophotometer, will measure photometric and radiometric BT(R)DFs for radiation of 380 to 1700 nanometer wavelengths, encompassing much of the solar spectrum. The device incorporates spectroradiometrically calibrated digital cameras and absorption filters to gather quasi-spectral information about reflection and transmission by complex fenestration systems. It relies on a half-mirrored, aluminum coated acrylic hemi-ellipsoid to project reflected or transmitted light towards a digital camera.(cont.) The device will be able to characterize BT(R)DFs for a variety of fenestration system materials, assemblies, and building materials. The goal of this research is to support the development of innovative, spectrally and angularly selective window systems that can improve daylighting and comfort and/or reduce cooling and heating loads in buildings. This thesis focuses on calibrating digital cameras to measure radiances with unknown spectra, developing the hemi-ellipsoid for the new goniophotometer, and developing methods for constructing quasi-spectral BT(R)DFs using this new device. The calibrated cameras also have potential for use in other applications, for example, as radiometers and photometers in rooms with light of known spectra.by Nicholas Gayeski.S.M

MODIS. Volume 2: MODIS level 1 geolocation, characterization and calibration algorithm theoretical basis document, version 1

The EOS Moderate Resolution Imaging Spectrometer (MODIS) is being developed by NASA for flight on the Earth Observing System (EOS) series of satellites, the first of which (EOS-AM-1) is scheduled for launch in 1998. This document describes the algorithms and their theoretical basis for the MODIS Level 1B characterization, calibration, and geolocation algorithms which must produce radiometrically, spectrally, and spatially calibrated data with sufficient accuracy so that Global change research programs can detect minute changes in biogeophysical parameters. The document first describes the geolocation algorithm which determines geodetic latitude, longitude, and elevation of each MODIS pixel and the determination of geometric parameters for each observation (satellite zenith angle, satellite azimuth, range to the satellite, solar zenith angle, and solar azimuth). Next, the utilization of the MODIS onboard calibration sources, which consist of the Spectroradiometric Calibration Assembly (SRCA), Solar Diffuser (SD), Solar Diffuser Stability Monitor (SDSM), and the Blackbody (BB), is treated. Characterization of these sources and integration of measurements into the calibration process is described. Finally, the use of external sources, including the Moon, instrumented sites on the Earth (called vicarious calibration), and unsupervised normalization sites having invariant reflectance and emissive properties is treated. Finally, algorithms for generating utility masks needed for scene-based calibration are discussed. Eight appendices are provided, covering instrument design and additional algorithm details

Toward 1% Photometry: End-to-end Calibration of Astronomical Telescopes and Detectors

We review the systematic uncertainties that have plagued attempts to obtain high precision and high accuracy from ground-based photometric measurements using CCDs. We identify two main challenges in breaking through the 1% precision barrier: 1) fully characterizing atmospheric transmission, along the instrument's line of sight, and 2) properly identifying, measuring and removing instrumental artifacts. We discuss approximations and limitations inherent in the present methodology, and we estimate their contributions to systematic photometric uncertainties. We propose an alternative conceptual scheme for the relative calibration of astronomical apparatus: the availability of calibrated detectors whose relative spectral sensitivity is known to better than one part in $10^3$ opens up the possibility of in situ relative throughput measurements, normalized to a precision calibrated detector, using a stable but uncalibrated narrowband light source. An implementation scheme is outlined, which exploits the availability of tunable lasers to map out the relative wavelength response of an imaging system, using a flatfield screen and a calibrated reference photodiode. The merits and limitations of this scheme are discussed. In tandem with careful measurements of atmospheric transmission, this approach could potentially lead to reliable ground-based photometry with fractional uncertainties below the percent level.Comment: 25 pages, no figures. To be published in Ap

Digital Color Imaging

This paper surveys current technology and research in the area of digital color imaging. In order to establish the background and lay down terminology, fundamental concepts of color perception and measurement are first presented us-ing vector-space notation and terminology. Present-day color recording and reproduction systems are reviewed along with the common mathematical models used for representing these devices. Algorithms for processing color images for display and communication are surveyed, and a forecast of research trends is attempted. An extensive bibliography is provided