Construction of membrane-bound artificial cells using microfluidics: a new frontier in bottom-up synthetic biology

The quest to construct artificial cells from the bottom-up using simple building blocks has received much attention over recent decades and is one of the grand challenges in synthetic biology. Cell mimics that are encapsulated by lipid membranes are a particularly powerful class of artificial cells due to their biocompatibility and the ability to reconstitute biological machinery within them. One of the key obstacles in the field centres on the following: how can membrane-based artificial cells be generated in a controlled way and in high-throughput? In particular, how can they be constructed to have precisely defined parameters including size, biomolecular composition and spatial organization? Microfluidic generation strategies have proved instrumental in addressing these questions. This article will outline some of the major principles underpinning membrane-based artificial cells and their construction using microfluidics, and will detail some recent landmarks that have been achieved

Roadmap on semiconductor-cell biointerfaces.

This roadmap outlines the role semiconductor-based materials play in understanding the complex biophysical dynamics at multiple length scales, as well as the design and implementation of next-generation electronic, optoelectronic, and mechanical devices for biointerfaces. The roadmap emphasizes the advantages of semiconductor building blocks in interfacing, monitoring, and manipulating the activity of biological components, and discusses the possibility of using active semiconductor-cell interfaces for discovering new signaling processes in the biological world

UltraSwarm: A Further Step Towards a Flock of Miniature Helicopters

We describe further progress towards the development of a MAV (micro aerial vehicle) designed as an enabling tool to investigate aerial flocking. Our research focuses on the use of low cost off the shelf vehicles and sensors to enable fast prototyping and to reduce development costs. Details on the design of the embedded electronics and the modification of the chosen toy helicopter are presented, and the technique used for state estimation is described. The fusion of inertial data through an unscented Kalman filter is used to estimate the helicopter’s state, and this forms the main input to the control system. Since no detailed dynamic model of the helicopter in use is available, a method is proposed for automated system identification, and for subsequent controller design based on artificial evolution. Preliminary results obtained with a dynamic simulator of a helicopter are reported, along with some encouraging results for tackling the problem of flocking

Dynamical principles in neuroscience

Dynamical modeling of neural systems and brain functions has a history of success over the last half century. This includes, for example, the explanation and prediction of some features of neural rhythmic behaviors. Many interesting dynamical models of learning and memory based on physiological experiments have been suggested over the last two decades. Dynamical models even of consciousness now exist. Usually these models and results are based on traditional approaches and paradigms of nonlinear dynamics including dynamical chaos. Neural systems are, however, an unusual subject for nonlinear dynamics for several reasons: (i) Even the simplest neural network, with only a few neurons and synaptic connections, has an enormous number of variables and control parameters. These make neural systems adaptive and flexible, and are critical to their biological function. (ii) In contrast to traditional physical systems described by well-known basic principles, first principles governing the dynamics of neural systems are unknown. (iii) Many different neural systems exhibit similar dynamics despite having different architectures and different levels of complexity. (iv) The network architecture and connection strengths are usually not known in detail and therefore the dynamical analysis must, in some sense, be probabilistic. (v) Since nervous systems are able to organize behavior based on sensory inputs, the dynamical modeling of these systems has to explain the transformation of temporal information into combinatorial or combinatorial-temporal codes, and vice versa, for memory and recognition. In this review these problems are discussed in the context of addressing the stimulating questions: What can neuroscience learn from nonlinear dynamics, and what can nonlinear dynamics learn from neuroscience?This work was supported by NSF Grant No. NSF/EIA-0130708, and Grant No. PHY 0414174; NIH Grant No. 1 R01 NS50945 and Grant No. NS40110; MEC BFI2003-07276, and Fundación BBVA

Mathematical analysis of scheduling policies in peer-to-peer video streaming networks

Las redes de pares son comunidades virtuales autogestionadas, desarrolladas en la capa de aplicación sobre la infraestructura de Internet, donde los usuarios (denominados pares) comparten recursos (ancho de banda, memoria, procesamiento) para alcanzar un fin común. La distribución de video representa la aplicación más desafiante, dadas las limitaciones de ancho de banda. Existen básicamente tres servicios de video. El más simple es la descarga, donde un conjunto de servidores posee el contenido original, y los usuarios deben descargar completamente este contenido previo a su reproducción. Un segundo servicio se denomina video bajo demanda, donde los pares se unen a una red virtual siempre que inicien una solicitud de un contenido de video, e inician una descarga progresiva en línea. El último servicio es video en vivo, donde el contenido de video es generado, distribuido y visualizado simultáneamente. En esta tesis se estudian aspectos de diseño para la distribución de video en vivo y bajo demanda. Se presenta un análisis matemático de estabilidad y capacidad de arquitecturas de distribución bajo demanda híbridas, asistidas por pares. Los pares inician descargas concurrentes de múltiples contenidos, y se desconectan cuando lo desean. Se predice la evolución esperada del sistema asumiendo proceso Poisson de arribos y egresos exponenciales, mediante un modelo determinístico de fluidos. Un sub-modelo de descargas secuenciales (no simultáneas) es globalmente y estructuralmente estable, independientemente de los parámetros de la red. Mediante la Ley de Little se determina el tiempo medio de residencia de usuarios en un sistema bajo demanda secuencial estacionario. Se demuestra teóricamente que la filosofía híbrida de cooperación entre pares siempre desempeña mejor que la tecnología pura basada en cliente-servidor