Histopathological image analysis : a review

Over the past decade, dramatic increases in computational power and improvement in image analysis algorithms have allowed the development of powerful computer-assisted analytical approaches to radiological data. With the recent advent of whole slide digital scanners, tissue histopathology slides can now be digitized and stored in digital image form. Consequently, digitized tissue histopathology has now become amenable to the application of computerized image analysis and machine learning techniques. Analogous to the role of computer-assisted diagnosis (CAD) algorithms in medical imaging to complement the opinion of a radiologist, CAD algorithms have begun to be developed for disease detection, diagnosis, and prognosis prediction to complement the opinion of the pathologist. In this paper, we review the recent state of the art CAD technology for digitized histopathology. This paper also briefly describes the development and application of novel image analysis technology for a few specific histopathology related problems being pursued in the United States and Europe

Two-photon imaging and analysis of neural network dynamics

The glow of a starry night sky, the smell of a freshly brewed cup of coffee or the sound of ocean waves breaking on the beach are representations of the physical world that have been created by the dynamic interactions of thousands of neurons in our brains. How the brain mediates perceptions, creates thoughts, stores memories and initiates actions remains one of the most profound puzzles in biology, if not all of science. A key to a mechanistic understanding of how the nervous system works is the ability to analyze the dynamics of neuronal networks in the living organism in the context of sensory stimulation and behaviour. Dynamic brain properties have been fairly well characterized on the microscopic level of individual neurons and on the macroscopic level of whole brain areas largely with the help of various electrophysiological techniques. However, our understanding of the mesoscopic level comprising local populations of hundreds to thousands of neurons (so called 'microcircuits') remains comparably poor. In large parts, this has been due to the technical difficulties involved in recording from large networks of neurons with single-cell spatial resolution and near- millisecond temporal resolution in the brain of living animals. In recent years, two-photon microscopy has emerged as a technique which meets many of these requirements and thus has become the method of choice for the interrogation of local neural circuits. Here, we review the state-of-research in the field of two-photon imaging of neuronal populations, covering the topics of microscope technology, suitable fluorescent indicator dyes, staining techniques, and in particular analysis techniques for extracting relevant information from the fluorescence data. We expect that functional analysis of neural networks using two-photon imaging will help to decipher fundamental operational principles of neural microcircuits.Comment: 36 pages, 4 figures, accepted for publication in Reports on Progress in Physic

Techniques and software tool for 3D multimodality medical image segmentation

The era of noninvasive diagnostic radiology and image-guided radiotherapy has witnessed burgeoning interest in applying different imaging modalities to stage and localize complex diseases such as atherosclerosis or cancer. It has been observed that using complementary information from multimodality images often significantly improves the robustness and accuracy of target volume definitions in radiotherapy treatment of cancer. In this work, we present techniques and an interactive software tool to support this new framework for 3D multimodality medical image segmentation. To demonstrate this methodology, we have designed and developed a dedicated open source software tool for multimodality image analysis MIASYS. The software tool aims to provide a needed solution for 3D image segmentation by integrating automatic algorithms, manual contouring methods, image preprocessing filters, post-processing procedures, user interactive features and evaluation metrics. The presented methods and the accompanying software tool have been successfully evaluated for different radiation therapy and diagnostic radiology applications

A SURVEY ON IMAGE SEGMENTATION USING DECISION FUSION METHOD

Neonatal brain MRI segmentation is challenging due to the poor image quality. Existing population atlases used for guiding segmentation are usually constructed by averaging all images in a population with no preference. However, such approaches diminish the important local inter-subject structural variability. Tissue segmentation of neonatal brain MR images remains challenging because of the insufficient image quality due to the properties of developing tissues. Among various brain tissue segmentation algorithms, atlas-based brain image segmentation can potentially achieve good segmentation results on neonatal brain images. Atlas-based segmentation approaches have been widely used for guiding brain tissue segmentation. Existing brain atlases are usually constructed by equally averaging presegmented images in a population. However, such approaches diminish local inter-subject structural variability and thus lead to lower segmentation guidance capability. To deal with this problem, we propose a multi-region-multi-reference framework for atlas-based neonatal brain segmentation