Can't touch this: the first-person perspective provides privileged access to predictions of sensory action outcomes.

RCUK Open Access funded. ESRC ES/J019178/1Previous studies have shown that viewing others in pain activates cortical somatosensory processing areas and facilitates the detection of tactile targets. It has been suggested that such shared representations have evolved to enable us to better understand the actions and intentions of others. If this is the case, the effects of observing others in pain should be obtained from a range of viewing perspectives. Therefore, the current study examined the behavioral effects of observed grasps of painful and nonpainful objects from both a first- and third-person perspective. In the first-person perspective, a participant was faster to detect a tactile target delivered to their own hand when viewing painful grasping actions, compared with all nonpainful actions. However, this effect was not revealed in the third-person perspective. The combination of action and object information to predict the painful consequences of another person's actions when viewed from the first-person perspective, but not the third-person perspective, argues against a mechanism ostensibly evolved to understand the actions of others

Review: Do the Different Sensory Areas within the Cat Anterior Ectosylvian Sulcal Cortex Collectively Represent a Network Multisensory Hub?

Current theory supports that the numerous functional areas of the cerebral cortex are organized and function as a network. Using connectional databases and computational approaches, the cerebral network has been demonstrated to exhibit a hierarchical structure composed of areas, clusters and, ultimately, hubs. Hubs are highly connected, higher-order regions that also facilitate communication between different sensory modalities. One region computationally identified network hub is the visual area of the Anterior Ectosylvian Sulcal cortex (AESc) of the cat. The Anterior Ectosylvian Visual area (AEV) is but one component of the AESc that also includes the auditory (Field of the Anterior Ectosylvian Sulcus - FAES) and somatosensory (Fourth somatosensory representation - SIV). To better understand the nature of cortical network hubs, the present report reviews the biological features of the AESc. Within the AESc, each area has extensive external cortical connections as well as among one another. Each of these core representations is separated by a transition zone characterized by bimodal neurons that share sensory properties of both adjoining core areas. Finally, core and transition zones are underlain by a continuous sheet of layer 5 neurons that project to common output structures. Altogether, these shared properties suggest that the collective AESc region represents a multiple sensory/multisensory cortical network hub. Ultimately, such an interconnected, composite structure adds complexity and biological detail to the understanding of cortical network hubs and their function in cortical processing

Evaluation of behavior in transgenic mouse models to understand human congenital pain conditions

BACKGROUND: Containing a brain for signal processing and decision making, and a peripheral component for sensation and response, the nervous system provides higher organisms a powerful method of interacting with their environment. The specific neurons involved in pain sensation are known as nociceptors and are the source of normal nociceptive pain signaling to prompt appropriate responses. Though acute hypersensitization can be advantageous by encouraging an organism to allow an injured area to heal, chronic pain conditions can be pathological and can markedly reduce quality of life. While a variety of genes have been associated with congenital pain conditions, two rare cases examined in this study have not had their mutated genes identified. Potassium voltage-gated channel subfamily H member 8, or KCNH8, is involved in regulating action potential production and propagation, and has not been linked with pain processing of any kind to date. Here, a male patient evaluated at Boston Children’s Hospital contains a novel single-base KCNH8 mutation and possesses an extremely low sensitivity to cold temperatures and mechanical pain, but a higher sensitivity to warmer temperatures. A separate protein, intersectin-2, or ITSN2, normally functions in clathrin-mediated endocytosis and exocytosis. A second patient at Boston Children’s Hospital expresses a previously-unseen point mutation in ITSN2 and experiences erythromelalgia, characterized by episodes of intense pain and red, swollen limbs during ambient warm temperatures. Through the use of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 genome editing, this study will produce these specific genetic mutations in mouse lines to explore their effects on mammalian behavior. OBJECTIVES: This project employs two transgenic mouse models to study the behavioral phenotypes associated with rare potentially damaging mutations in KCNH8 and ITSN2 exhibited in the human patients. Through these experiments, a greater understanding of neural pain signaling and sensitivity changes can occur. METHODS: The differences in temperature preference of KCNH8 and ITSN2 mutant mice compared to wild type mice lacking these mutations was studied using thermal plates under cold and warm conditions. Direct application of acetone and von Frey filaments to mouse paws was used to study cold and mechanical sensitivity. Further testing of stamina, anxiety, coordination, and strength were also evaluated. RESULTS: A marked decrease in sensitivity to von Frey stimulation (p<0.01) and acetone administration (p<0.05) was observed in KCNH8 mutant mice. Thermal preference testing demonstrated a decreased preference for warmer temperatures as compared to wild type mice. In addition, anxiety levels were also observed to be slightly higher in these mutant KCNH8 mice (p<0.05). The mutant ITSN2 mice spent less time at cooler temperatures, though surprisingly they significantly preferred warmer conditions as compared to their wild type littermates. A full and partial reversal of these temperature preferences was demonstrated in cold and heat thermal conditions respectively after intraperitoneal gabapentin injection, which normalized the mice toward wild type behavior. CONCLUSIONS: Data from the KCNH8 mutant mouse model indicates an aversion to warmer temperatures and a decreased ability to detect cold or mechanical pressure, much like the human patient. The mutant ITSN2 mice were less likely to spend time at cooler temperatures, indicating heightened sensory sensitivity, but their preference for warmer temperatures suggests a possible desensitization of the affected nociceptors. These results often mirror the patient’s phenotype, but the preference for ambient warmer environments appears opposite to the patient. As the ITSN2 mice feel discomfort at cooler temperatures, a proposed desensitization at warmer temperatures would result in a more comfortable environment and could explain the observed preference. The trends toward normal neural firing rates achieved through gabapentin injection suggest that the aberrant responses in mutant ITSN2 mice is due to altered sensitization, but additional examination under these conditions with a larger group of mice is necessary to further unravel these signaling pathways. However, these extremely encouraging data introduce two new molecular targets for acute pain control

Engineering data compendium. Human perception and performance. User's guide

The concept underlying the Engineering Data Compendium was the product of a research and development program (Integrated Perceptual Information for Designers project) aimed at facilitating the application of basic research findings in human performance to the design and military crew systems. The principal objective was to develop a workable strategy for: (1) identifying and distilling information of potential value to system design from the existing research literature, and (2) presenting this technical information in a way that would aid its accessibility, interpretability, and applicability by systems designers. The present four volumes of the Engineering Data Compendium represent the first implementation of this strategy. This is the first volume, the User's Guide, containing a description of the program and instructions for its use

Sensory coding in supragranular cells of the vibrissal cortex in anesthetized and awake mice

Sensory perception entails reliable representation of the external stimuli as impulse activity of individual neurons (i.e. spikes) and neuronal populations in the sensory area. An ongoing challenge in neuroscience is to identify and characterize the features of the stimuli which are relevant to a specific sensory modality and neuronal strategies to effectively and efficiently encode those features. It is widely hypothesized that the neuronal populations employ “sparse coding” strategies to optimize the stimulus representations with a low energetic cost (i.e. low impulse activity). In the past two decades, a wealth of experimental evidence has supported this hypothesis by showing spatiotemporally sparse activity in sensory area. Despite numerous studies, the extent of sparse coding and its underlying mechanisms are not fully understood, especially in primary vibrissal somatosensory cortex (vS1), which is a key model system in sensory neuroscience. Importantly, it is not clear yet whether sparse activation of supragranular vS1 is due to insufficient synaptic input to the majority of the cells or the absence of effective stimulus features. In this thesis, first we asked how the choice of stimulus could affect the degree of sparseness and/or the overall fraction of the responsive vS1 neurons. We presented whisker deflections spanning a broad range of intensities, including “standard stimuli” and a high-velocity, “sharp” stimulus, which simulated the fast slip events that occur during whisker mediated object palpation. We used whole-cell and cell-attached recording and calcium imaging to characterize the neuronal responses to these stimuli. Consistent with previous literature, whole-cell recording revealed a sparse response to the standard range of velocities: although all recorded cells showed tuning to velocity in their postsynaptic potentials, only a small fraction produced stimulus-evoked spikes. In contrast, the sharp stimulus evoked reliable spiking in a large fraction of regular spiking neurons in the supragranular vS1. Spiking responses to the sharp stimulus were binary and precisely timed, with minimum trial-to-trial variability. Interestingly, we also observed that the sharp stimulus produced a consistent and significant reduction in action potential threshold. In the second step we asked whether the stimulus dependent sparse and dense activations we found in anesthetized condition would generalize to the awake condition. We employed cell-attached recordings in head-fixed awake mice to explore the degree of sparseness in awake cortex. Although, stimuli delivered by a piezo-electric actuator evoked significant response in a small fraction of regular spiking supragranular neurons (16%-29%), we observed that a majority of neurons (84%) were driven by manual probing of whiskers. Our results demonstrate that despite sparse activity, the majority of neurons in the superficial layers of vS1 contribute to coding by representing a specific feature of the tactile stimulus. Thesis outline: Chapter 1 provides a review of the current knowledge on sparse coding and an overview of the whisker-sensory pathway. Chapter 2 represents our published results regarding sparse and dense coding in vS1 of anesthetized mice (Ranjbar-Slamloo and Arabzadeh 2017). Chapter 3 represents our pending manuscript with results obtained with piezo and manual stimulation in awake mice. Finally, in Chapter 4 we discuss and conclude our findings in the context of the literature. The appendix provides unpublished results related to Chapter 2. This section is referenced in the final chapter for further discussion

Electroencephalographic Responses to Frictional Stimuli: Measurement Setup and Processing Pipeline

Tactility is a key sense in the human interaction with the environment. The understanding of tactile perception has become an exciting area in industrial, medical and scienti c research with an emphasis on the development of new haptic technologies. Surprisingly, the quanti cation of tactile perception has, compared to other senses, only recently become a eld of scienti c investigation. The overall goal of this emerging scienti c discipline is an understanding of the causal chain from the contact of the skin with materials to the brain dynamics representing recognition of and emotional reaction to the materials. Each link in this chain depends on individual and environmental factors ranging from the in uence of humidity on contact formation to the role of attention for the perception of touch. This thesis reports on the research of neural correlates to the frictional stimulation of the human ngertip. Event-related electroencephalographic potentials (ERPs) upon the change in ngertip friction are measured and studied, when pins of a programmable Braille-display were brought into skin contact. In order to contribute to the understanding of the causal chain mentioned above, this work combines two research areas which are usually not connected to each other, namely tribology and neuroscience. The goal of the study is to evaluate contributions of friction to the process of haptic perception. Key contributions of this thesis are: 1) Development of a setup to simultaneously record physical forces and ERPs upon tactile stimulation. 2) Implementation of a dedicated signal processing pipeline for the statistical analysis of ERP -amplitudes, -latencies and -instantaneous phases. 3) Interpretation of skin friction data and extraction of neural correlates with respect to varying friction intensities. The tactile stimulation of the ngertip upon raising and lowering of di erent lines of Braille-pins (one, three and ve) caused pronounced N50 and P100 components in the event-related ERPsequences, which is in line with the current literature. Friction between the ngertip and the Braille-system exhibited a characteristic temporal development which is attributed to viscoelastic skin relaxation. Although the force stimuli varied by a factor of two between the di erent Braillepatterns, no signi cant di erences were observed between the amplitudes and latencies of ERPs after standard across-trial averaging. Thus, for the rst time a phase measure for estimating singletrial interactions of somatosensory potentials is proposed. Results show that instantaneous phase coherency is evoked by friction, and that higher friction induces stronger and more time-localized phase coherencyDie Taktilität ist ein zentraler Sinn in der Interaktion mit unserer Umwelt. Das Bestreben, fundierte Erkenntnisse hinsichtlich der taktilenWahrnehmung zu gewinnen erhält groÿen Zuspruch in der industriellen, medizinischen und wissenschaftlichen Forschung, meist mit einem Fokus auf der Entwicklung von haptischen Technologien. Erstaunlicherweise ist jedoch die wissenschaftliche Quanti zierung der taktilen Wahrnehmung, verglichen mit anderen Sinnesmodalitäten, erst seit kurzem ein sich entwickelnder Forschungsbereich. Fokus dieser Disziplin ist es, die kognitive und emotionale Reaktion nach physischem Kontakt mit Materialien zu beschreiben, und die kausale Wirkungskette von der Berührung bis zur Reaktion zu verstehen. Dabei unterliegen die einzelnen Faktoren dieser Kette sowohl individuellen als auch externen Ein üssen, welche von der Luftfeuchtigkeit während des Kontaktes bis hin zur Rolle der Aufmerksamkeit für die Wahrnehmung reichen. Die vorliegende Arbeit beschäftigt sich mit der Untersuchung von neuronalen Korrelaten nach Reibungsstimulation des menschlichen Fingers. Dazu wurden Reibungsänderungen, welche durch den Kontakt der menschlichen Fingerspitze mit schaltbaren Stiften eines Braille-Display erzeugt wurden, untersucht und die entsprechenden neuronalen Korrelate aufgezeichnet. Um zu dem Verst ändnis der oben erwähnten Wirkungskette beizutragen, werden Ansätze aus zwei für gewöhnlich nicht zusammenhängenden Forschungsbereichen, nämlich der Tribologie und der Neurowissenschaft, kombiniert. Folgende Beiträge sind Hauptbestandteile dieser Arbeit: 1) Realisierung einer Messumgebung zur simultanen Ableitung von Kräften und ereigniskorrelierten Potentialen nach taktiler Stimulation der Fingerspitze. 2) Aufbau einer speziellen Signalverarbeitungskette zur statistischen Analyse von stimulationsabh ängigen EEG -Amplituden, -Latenzen und -instantanen Phasen. 3) Interpretation der erhobenen Reibungsdaten und Extraktion neuronaler Korrelate hinsichtlich variierender Stimulationsintensitäten. Unsere Resultate zeigen, dass die taktile Stimulation der Fingerspitze nach Anheben und Senken von Braille-Stiften zu signi kanten N50 und P100 Komponenten in den ereigniskorrelierten Potentialen führt, im Einklang mit der aktuellen Literatur. Die Reibung zwischen der Fingerspitze und dem Braille-System zeigte einen charakteristischen Signalverlauf, welcher auf viskoelastische Hautrelaxation zurückzuführen ist. Trotz der um einen Faktor zwei verschiedenen Intensit ätsunterschiede zwischen den Stimulationsmustern zeigten sich keine signi kanten Unterschiede zwischen den einfach gemittelten Amplituden der evozierten Potentialen. Erstmalig wurde ein Phasen-Maÿ zur Identi zierung von Unterschieden zwischen somatosensorischen "single-trial" Interaktionen angewandt. Diese Phasenanalyse zeigte, im Gegensatz zur Amplituden- und Latenzanalyse, deutlichere und signi kantere Unterschiede zwischen den Stimulationsparadigmen. Es wird gefolgert, dass Kohärenz zwischen den Momentanphasen durch Reibungsereignisse herbeigef ührt wird und dass durch stärkere Reibung diese Kohärenz, im zeitlichen Verlauf, stärker und lokalisierter wird

Perception of patterned vibratory stimulation: An evaluation of the tactile vision substitution system

Sensory substitution--The replacing of an impaired sensory channel by a properly functioning one--is possibly best manifested today in attempts to provide visual aids for the blind. The tactile vision substitution system (T.V.S.S.) is an example of one such visual aid. The system presents patterned tactile stimulation to the skin of the observer provided by the output of a closed-circuit television system. Research conducted with congenitally blind Ss in evaluation of the T.V.S.S. has provided useful information concerning the potentialities and limitations of the prototype systems, similarities and differences between tactile and visual perception, and the development of visual perception in the congenitally blind Investigation demonstrated that the congenitally blind Ss can learn to make valid judgements of three-dimensional displays with the T.V.S.S. Such judgements are made on the basis of properties contained in the proximal stimulation properties analogous to the monocular clues of depth presence in vision, such as linear-perspective, apparent elevation in the visual field, size change as a function of distance, occlusion, and texural gradients. Similarities have been noted between judgements made by sighted Ss using vision and by blind Ss using the T.V.S.S. on comparable tasks. A display consisting of two slightly displaced alternating lights is perceived in both situations as a single spot of light moving back-and-forth between two display boundaries. A rotating drum made up of alternate black and white stripes is, when stopped, perceived as briefly moving in the opposite direction. External localization of the source of stimulation also occurs with both sensory inputs. The major differences between the visual and tactile inputs that have been noted have occurred in form recognition tacks. Although blind Ss using the patterned tactile stimulation are able to identify both geometric forms and abstract patterns, accuracy is consistently lower than that of sighted Ss using vision, and the latencies for the blind Ss are significantly longer. It is hypothesized that the longer latencies for the blind Ss using the T.V.S.S. can be accounted for primarily by the need to hand-position the television camera during scanning. A major factor in the lower accuracy for the tactile group is the noted difficulty in detecting and identifying display features located within a mass of stimulation. This difficulty with internal display detail may be a function of sensory inhibition and/or masking. The research findings support a concept of sensory substitution as well as a theory of perception which stresses the modality of many qualities contained in visible displays. Further research is needed to determine the significance of sensor movement--either eye movements or camera manipulation--in the perceptual process